|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Protective action of glutamine in rats with severe acute liver failure显示文摘BACKGROUND Severe acute liver failure(SALF) is a rare, but high-mortality, rapidly evolving syndrome that leads to hepatocyte degeneration with impaired liver function.Thioacetamide(TAA) is a known xenobiotic, which promotes the increase of the formation of reactive oxygen species. Erythroid 2-related factor 2(Nrf2) activates the antioxidant protection of cells. Studies have evidenced the involvement of inflammatory mediators in conditions of oxidative stress.AIM To evaluate the antioxidant effects of glutamine on Nrf2 activation and NFκBmediated inflammation in rats with TAA-induced IHAG.METHODS Male Wistar rats(n = 28) were divided into four groups: control,control+glutamine, TAA, and TAA + glutamine. Two TAA doses(400 mg/kg)were administered intraperitoneally, 8 h apart. Glutamine(25 mg/kg) was administered at 30 min, 24 h, and 36 h. At 48 h, blood was collected for liver integrity analysis [aspartate aminotransferase(AST), alanine aminotransferase(ALT), and alkaline phosphatase(ALP)]. The liver was harvested for histology and assessment of oxidative stress [thiobarbituric acid-reactive substances(TBARS), catalase(CAT), glutathione peroxidase(GPx), glutathione S-transferase(GST), glutathione(GSH), Nrf2, Kelch-like ECH-associated protein 1(Keap1),NADPH quinone oxidoreductase1(NQO1), superoxide dismutase(SOD)] and inflammatory process.RESULTS TAA caused disruption of the hepatic parenchyma, with inflammatoryinfiltration, massive necrosis, and ballooning degeneration. Glutamine mitigated this tissue damage, with visible regeneration of hepatic parenchyma; decreased TBARS(P < 0.001), GSH(P < 0.01), IL-1β, IL6, and TNFα levels(P <0.01) in hepatic tissue; and decreased blood levels of AST, ALT, and ALP(P <0.05). In addition, CAT, GPx, and GST activities were restored in the glutamine group(P<0.01, P <0.01, and P <0.001, respectively vs TAA alone). Glutamine increased expression of Nrf2(P < 0.05), NQO1, and SOD(P < 0.01), as well as levels of IL-10(P <0.001), while decreasing expression of Keap1, TLR4, NFκB(P < 0.001), COX-2 and iNOS,(P < 0.01), and reducing NO_2 and NO_3 levels(P < 0.05).CONCLUSION In the TAA experimental model of IHAG, glutamine activated the Nrf2 pathway,thus promoting antioxidant protection, and blunted the NFκB-mediated pathway, reducing inflammation. | Elizangela G Schemitt Renata M Hartmann Josieli R Colares Francielli Licks Jéferson O Salvi Cláudio A Marroni Norma P Marroni | 2019 | World Journal of Hepatology2019，11，3： | 5 |
| 2 | Antioxidant and anti-inflammatory action of melatonin in an experimental model of secondary biliary cirrhosis induced by bile duct ligation显示文摘AIM To evaluate the effects of melatonin(Mel) on oxidative stress in an experimental model of bile duct ligation(BDL).METHODS Male Wistar rats(n = 32, weight ± 300 g) were allocated across four groups: CO(sham BDL), BDL(BDL surgery), CO + Mel(sham BDL and Mel administration) and BDL + Mel(BDL surgery and Mel administration). Mel was administered intraperitoneally for 2 wk, starting on postoperative day 15, at a dose of 20 mg/kg.RESULTS Mel was effective at the different standards, reestablishing normal liver enzyme levels, reducing the hepatosomatic and splenosomatic indices, restoring lipoperoxidation and antioxidant enzyme concentrations, reducing fibrosis and inflammation, and thereby reducing liver tissue injury in the treated animals.CONCLUSION The results of this study suggest a protective effect of Mel when administered to rats with secondary biliary cirrhosis induced by BDL. | Josieli Raskopf Colares Elizângela Goncalves Schemitt Renata Minuzzo Hartmann Francielli Licks Mariana do Couto Soares Adriane Dal Bosco Norma Possa Marroni | 2016 | World Journal of Gastroenterology2016，22，40： | 3 |
| 3 | N-acetylcysteine modulates angiogenesis and vasodilation in stomach such as DNA damage in blood of portal hypertensive rats显示文摘AIM: To evaluate the antioxidant effect of N-acetylcysteine(NAC) on the stomach of rats with portal hypertension.METHODS: Twenty-four male Wistar rats weighing ± 250 g were divided into four experimental groups(n =6 each): Sham-operated(SO),SO + NAC,partial portal vein ligation(PPVL),and PPVL + NAC. Treatment with NAC in a dose of 10 mg/kg(i.p.) diluted in 0.6 m L of saline solution was administered daily for 7 d starting 8 d after the surgery. Animals from the PPVL and SO group received saline solution(0.6 m L) for the same period of time as the PPVL + NAC and SO + NAC group. On the 15 th day the animals were anesthetized and we evaluated portal pressure by cannulating mesenteric artery. After,we removed the stomach for further analysis. We performed immunohistochemical analysis for endothelial nitric oxide synthase(e NOS),vascular endothelial growth factor(VEGF),and nitrotirosine(NTT) proteins in stomach. We also evaluated e NOS and VEGF by Western blot analysis and assessed DNA damage in blood samples by the comet assay.RESULTS: The portal hypertension group exhibited increases in portal pressure when compared to SO group(29.8 ± 1.8 vs 12.0 ± 0.3 mm Hg)(P < 0.001). The same was observed when we compared the e NOS(56.8 ± 3.7 vs 13.46 ± 2.8 pixels)(P < 0.001),VEGF(34.9 ± 4.7 vs 17.46 ± 2.6 pixels)(P < 0.05),and NTT(39.01 ± 4.0 vs 12.77 ± 2.3 pixels)(P < 0.05) expression by immunohistochemistry of the PPVL animals with the SO group. The expression of e NOS(0.39 ± 0.03 vs 0.25 ± 0.03 a.μ)(P < 0.01) and VEGF(0.38 ± 0.04 vs 0.26 ± 0.04 a.μ)(P < 0.01) were also evaluated by Western blot analysis,and we observed an increase of both proteins on PPVL animals. We also evaluated the DNA damage by comet assay,and observed an increase on damage index and damage frequency on those animals. NAC decreased portal pressure values in PPVL + NAC animals(16.46 ± 2 vs 29.8 ± 1.8 mm Hg)(P < 0.001) when compared to PPVL. The expression of e NOS(14.60 ± 4.1 vs 56.8 ± 3.7 pixels)(P < 0.001),VEGF(19.53 ± 3.2 vs 34.9 ± 4.7 pixels)(P < 0.05) and NTT(21.84 ± 0.7 vs 39.01 ± 4.0 pixels)(P < 0.05) evaluated by immunohistochemistry were also reduced in PPVL + NAC animals. Also,when evaluated by Western blot e NOS expression(0.32 ± 0.03 vs 0.39 ± 0.03 a.μ)(P < 0.05) and VEGF expression(0.31 ± 0.09 vs 0.38 ± 0.04 a.μ)(P < 0.01). Furthermore,NAC modulated DNA damage in PPVL + NAC animals.CONCLUSION: In view of these results,we believe NAC is able to protect the stomach from the alterations induced by the PPVL procedure. | Francielli Licks Renata Minuzzo Hartmann Camila Marques Elizangela Schemitt Josieli Raskopf Colares Mariana do Couto Soares Juliana Reys Camila Fisher Juliana da Silva Norma Possa Marroni | 2015 | World Journal of Gastroenterology2015，21，43： | 2 |
| 4 | Content levels of various fruit metabolites in the 'Conference' pear response to branch ben- ding显示文摘 | COLARIC M STAMPAR F HUDINA M | 2007 | Scientia Horticulturae2007，113，3： | 1 |
| 5 | Behavior of natural fiber in stone matrix asphalt mixtures using two design methods显示文摘 | VALE D COLARES A CASAGRANDE M et a| | 2014 | Journal of Materials in Civil Engineering2014，26，3： | 1 |
| 6 | A gap theorem for complete scalar curvature hypersurfaces in the de Sitter space显示文摘 | Colares A G Palmas O | 2001 | J Geom Physics2001，37，： | 1 |
| 7 | Traditional walnut liqueur – cocktail of phenolics 显示文摘 | Stampar F Solar A Hudina M Veberic R Colaric M | 2006 | Food Chem2006，95，4： | 1 |
| 8 | Factors associated with uncooperativebehavior by Brazilian preschool children in the dental office显示文摘 | Colares V Richman LS | 2002 | ASDC J Dent Child2002，69，1： | 1 |
| 9 | A further characterization of ellipsoids显示文摘 | Alias L J Colares A G | 2005 | Results in Mathematics2005，48，： | 1 |
| 10 | Stability of hypersurfaces with constant r-mean curvature显示文摘 | Barbosa J L M Colares A G | 1997 | Annals of Global Analysis and Geometry1997，15，： | 1 |
| 11 | Factors associated with uncooperativebehavior by brazilian preschool children in the dental office 显示文摘 | Colares Y Richman L | 2002 | ASDC J Dent Child2002，69，1： | 1 |
| 12 | Pleiotropic effects of statins on the treatment of chronic periodontitis – a systematic review显示文摘 | Ilanna Mara Gomes Estanislau Icrólio Ribeiro Colares Terceiro Mario Roberto Pontes Lisboa Patrícia de Barros Teles Rosimary de Sousa Carvalho Ricardo Souza Martins Maria M?nica Studart Mendes Moreira | 2015 | Br J Clin Pharmacol2015，，： | 1 |
| 13 | Bending affects pheno-lic content of William pear leaves显示文摘 | COLARIC M STAMPAR F HUDINA M | 2007 | Plant Soil Science2007，57，2： | 1 |
| 14 | Behavior of Natural Fiber in Stone Matrix Asphalt MixturesUsing Two Design Methods 显示文摘 | Aline Colares do Vale Michele Dal Toe Casagrande Jorge Barbosa Soares | 2014 | American Society of Civil Engineers2014，26，： | 1 |
| 15 | A brief history of Brazilian biofuel legislation 显示文摘 | JUSCELINO F COLARES | 2008 | Syracuse journal of international law and commerce2008，，6： | 1 |
| 16 | A gap theorem for complete scalar curvature hypersurfaces in the de Sitter space显示文摘 | Colares A G Palmas O | 2001 | J Geom Physics2001，37，： | 1 |
| 17 | Seasonal variations of se- lected flavonoids, phenolic acids and quinones in annual shoots of com- mon walnut (Juglans regia L)显示文摘 | SOLAR A COLARIC M USENIK V | 2006 | Plant Sci2006，170，3： | 1 |
| 18 | Synergistic antibiotic activity of volatile compounds from the essential oil of Lippia sidoides and thymol显示文摘 | Helenicy N.H. Veras Fabíola F.G. Rodrigues Aracélio V. Colares Irwin R.A. Menezes Henrique D.M. Coutinho Marco A. Botelho José G.M. Costa | 2011 | Fitoterapia2011，，3： | 1 |
| 19 | Cross-sectional study to determine viral hepatitis knowledge in different urban populations in Brazil显示文摘AIM To evaluate viral hepatitis knowledge among individuals from different resource areas and health conditions to identify possible gaps.METHODS A cross-sectional, descriptive study was carried out among 447 individuals from five distinct populations in Brazil: Southeast Viral Hepatitis Ambulatory(n = 100), South(n = 89) and Northeast(n = 114) Health Center, Southeast(n = 77) and Northeast(n = 67) low resource areas. All individuals answered a questionnaire assessing sociodemographic characteristics and viral hepatitis awareness. The perception was scored based on the average number of correct answers of all participants and categorized as 'low'(0-28 correct answers) or 'desirable'(29-46 correct answers). Associations between sociodemographic characteristics and perception were also evaluated.RESULTS A low level of knowledge was observed in individuals from Northeast Health Center, Northeast and Southeast low resource areas while desirable knowledge was observed in individuals from Viral Hepatitis Ambulatory and South Health Center. According to sociodemographic characteristics, desirable scores were more common among those with secondary education(47.1%), those who declared themselves as white(46.3%), and those who lived in houses with three individuals(25.5%). Multivariate analysis showed an association between viral hepatitis perception and type of population. CONCLUSION The results demonstrated high level of knowledge among study participants from health clinics from the Southeast region of Brazil and the importance of education programs in increasing the level of knowledge in low resource areas. | Helena Medina Cruz Jakeline Ribeiro Barbosa Jeová Keny Baima Colares Antonio Henrique Almeida de Moraes Neto Maria de Fátima Leal Alencar Francisco Inácio Bastos Jurema Corrêa da Mota Filipe Aníbal Carvalho-Costa Claudia Alexandra Pontes Ivantes Lia Laura Lewis-Ximenez Livia Melo Villar | 2018 | World Journal of Hepatology2018，10，11： | 1 |
| 20 | A further characterization of ellipsoids显示文摘 | ALiAS L J COLARES A G | 2005 | Results Math2005，48，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员