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| 1 | Immunohistochemical expression of thymidylate synthase and prognosis in gastric cancer patients submitted to fluoropyrimidine-based chemotherapy显示文摘Objective: Adjuvant chemotherapy with 5-fluorouracil(5-FU) has been widely used in gastric cancer(GC)patients to prevent relapse after curative resection. 5-FU acts by inhibiting thymidylate synthase(TS), and high levels of TS correlate with resistance to treatment with fluoropyrimidines. The aim of this study was to evaluate the expression of TS in GC patients, and its relation with clinicopathological characteristics and prognosis in adjuvant chemotherapy with 5-FU.Methods: We retrospectively evaluated 285 patients who underwent D2-gastrectomy with curative intent. TS expression was determined by immunohistochemistry(IHC) in tumor cells by tissue microarray(TMA). TS level was evaluated according to the intensity and percentage of cells marked by a score system. Patients were divided in three groups according to their TS-score: negative, low and high.Results: TS expression was positive in 92.3% of GC. TS-high, TS-low and TS-negative were observed in46.3%, 46.0% and 7.7% of patients, respectively. High-TS GC were associated with older age(P=0.007), high neutrophil/lymphocyte ratio(P=0.048), well/moderately differentiated histology(P=0.001), intestinal Lauren type(P<0.001) and absence of perineural invasion(P=0.003). Among 285 patients, 133 stage Ⅱ/Ⅲ patients(46.7%)received chemotherapy with 5-FU. In survival analysis, TS-high was associated with worse disease-free survival(DFS) in stage Ⅲ GC patients who received 5-FU-based chemotherapy(P=0.007). Multivariate analysis revealed that total gastrectomy, poorly differentiated tumors and high TS-score were associated with worse DFS in stage Ⅲ GC patients.Conclusions: High TS-score in stage Ⅲ GC was associated with poor DFS in patients treated with fluoropyrimidine-based chemotherapy. | Marina Alessandra Pereira Marcus Fernando Kodama Pertille Ramos ANDre Roncon Dias Sheila Friedrich Faraj Cinthya dos Santos Cirqueira EvANDro Sobroza de Mello Bruno Zilberstein Venancio Avancini Ferreira Alves Ulysses Ribeiro Jr | 2018 | Chinese Journal of Cancer Research2018，30，5： | 3 |
| 2 | Thermogravimetric studies on the effect of support in sorbent-materials applied to flue gas desulfurization 显示文摘 | ROBERTO F ARTURO R CINTHYA P YULIANA M XOCHITL M LAURA G | 2008 | Fuel2008，87，： | 1 |
| 3 | Challenges and lessons learned from NOTES cholecystectomy initial experience: a stepwise approach from the laboratory to clinical application显示文摘 | Mitsuhiro Asakuma Silvana Perretta Pierre Allemann Ronan Cahill Sergio A. Con Cinthya Solano Shanker Pasupathy Didier Mutter Bernard Dallemagne Jacques Marescaux | 2009 | Journal of Hepato - Biliary - Pancreatic Surgery2009，，3： | 1 |
| 4 | Noisy Texture Classfifcation :A Higher- Order Statistics Approach显示文摘 | Vittorio M Cinthya O Sergio P | 1998 | Pattern Recognition1998，31，4： | 1 |
| 5 | Programmed cell deaths显示文摘 | Cinthya Assuncao Guimaraes Rafael Linden | 2004 | Eur J Biochem2004，271，： | 1 |
| 6 | Noisy texture classification: a higher-order statistics approach显示文摘 | Vittorio M Cinthya O Sergio P | 1998 | Pattern recognition1998，31，4： | 1 |
| 7 | Endotoxin-induced acute lung injury is dependent upon oxidative response显示文摘 | Eduardo Tavares Lima Trajano Cinthya Sternberg Maurí cio Caetano Marco Auré lio Santos Silva Luí s Cristó v? o Porto Juliana Carvalho Santos Marcelo Lima Ribeiro Clarissa Bichara Magalh? es Walter Araú jo Zin Clá udia Farias Benjamim Samuel Santos Valen? | 2011 | Inhalation Toxicology2011，，： | 1 |
| 8 | Relationship between oxidative stress and mitochondrial function in the post-conditioned heart显示文摘 | Francisco Correa Noemí García Cinthya Robles Eduardo Martínez-Abundis Cecilia Zazueta | 2008 | Journal of Bioenergetics and Biomembranes2008，，6： | 1 |
| 9 | Immunohistochemistry panel segregates molecular types of hepatocellular carcinoma in Brazilian autopsy cases显示文摘AIM: To assess the distribution of proteins coded by genes reported as relevant for the molecular classification of hepatocellular carcinoma(HCC).METHODS: In this retrospective cross-sectional study, the following clinicopathological data were analyzed in 80 autopsied HCC patients: sex, age, ethnicity, alcohol intake, infection with hepatitis B and/or C virus, infection with human immunodeficiency virus, prior treatment, basic and immediate causes of death, liver weight, presence of cirrhosis, number and size of nodules, gross pattern, histological grade and variants, architectural pattern, invasion of large veins, and presence and location of extrahepatic metastases. The protein products of genes known to be involved in molecular pathogenesis of HCC, including epidermal growth factor receptor(EGFR), MET, keratin 19(K19), vimentin, beta-catenin, mechanistic target of rapamycin(m TOR), extracellular signaling-relatedkinase(ERK)1, ERK2, Ki67, cyclin D1, caspase 3 and p53, were detected by immunohistochemistry on tissue microarrays. The expression levels were scored and statistically assessed for correlation with HCC parameters. RESULTS: Infection with hepatitis C virus was identified in 49% of the 80 autopsy patients, cirrhosis in 90%, advanced tumors in 95%, and extrahepatic metastases in 38%. Expression of K19, p53 and ERK1 correlated to high-grade lesions. Expression of ERK1, nuclear beta-catenin, cyclin D1 and ERK2 correlated to higher rates of cell proliferation as determined by Ki67. Expression of MET, EGFR(> 0) and caspase 3 correlated with lower histological grades. Expression o f E G F R c o r r e l a t e d t o t h a t o f c a s p a s e 3, a n d overexpression of EGFR(≥ 200/300) was observed in low-grade tumors more frequently(grades 1 and 2: 67% vs grade 3: 27% and grade 4: 30%). Expression of ERK1 was associated with that of K19 and vimentin, whereas expression of ERK2 was associated with that of cyclin D1, MET and membrane beta-catenin. Expression of vimentin was strongly correlated with that of K19. CONCLUSION: Expression of K19, p53, ERK1, ERK2, vimentin and nuclear beta-catenin was related to highergrade markers, as opposed to expression/overexpression of EGFR, MET and caspase 3. | Aloísio Felipe-Silva Alda Wakamatsu Cinthya dos Santos Cirqueira Venancio Avancini Ferreira Alves | 2016 | World Journal of Gastroenterology2016，22，27： | 0 |
| 10 | Study of the Action Mode of Wickerhamomyces anomalus against Colletotrichum gloeosporioides显示文摘 | Luis Femando Zepeda-Giraud Dario Rafael Olicon-Hemandez Cinthya Martinez-Lopez Guadalupe Guerra-Sanchez | 2016 | Journal of Agricultural Science and Technology(B)2016，6，5： | 0 |
| 11 | Gastric cancer molecular classification based on immunohistochemistry and in situ hybridization:Analysis in western patients after curative-intent surgery显示文摘BACKGROUND Gastric cancer(GC)is a highly heterogeneous disease,and the identification of molecular subtyping of gastric adenocarcinoma emerged as a promising option to define therapeutic strategies and prognostic subgroups.However,the costs and technical complexity of molecular methodologies remains an obstacle to its adoption,and their clinical significance by other approaches needs further evidence.AIM To evaluate the clinicopathological characteristics and long-term survival of GC based on the subgroups of molecular classification by immunohistochemistry(IHC)and in situ hybridization(ISH).METHODS We retrospectively evaluated all patients who underwent D2-gastrectomy between 2009 and 2016 in a Western cohort of GC patients treated with curative intent.Microsatellite instability(MSI)status,E-cadherin,and p53 expression were analyzed by IHC,and Epstein-Barr virus(EBV)by ISH.Tissue microarrays were constructed for analysis.Clinicopathological characteristics and survival of GC were evaluated according to subtypes defined by The Cancer Genome Atlas(TCGA)Research Network Group and Asian Cancer Research Group(ACRG)classification systems.RESULTS A total of 287 GC patients were included.Based on IHC and ISH analysis,five profiles were defined as follows:E-cadherin aberrant(9.1%),MSI(20.9%),p53 aberrant(36.6%),EBV positivity(10.5%),and p53 normal(31%),which corresponded to tumors that showed no alteration in another profile.A flowchart according to the TCGA and ACRG classifications were used to define the subtypes,where clinical and pathological characteristics associated with GC subtypes were evidenced.Proximal location(P<0.001),total gastrectomy(P=0.001),and intense inflammatory infiltrate(P<0.001)were characteristics related to EBV subtype.MSI subtype was predominantly associated with advanced age(P=0.017)and the presence of comorbidities(P=0.011).While Laurén diffuse type(P<0.001)and advanced stage(P=0.029)were related to genomically stable(GS)subtype.GS tumors and microsatellite stable/epithelial to mesenchymal transition phenotype subtype had worse disease-free survival(DFS)and overall survival(OS)than other subtypes.Conversely,MSI subtype of GC had better survival in both classifications.Type of gastrectomy,pT and the TCGA subtypes were independent factors associated to DFS and OS.CONCLUSION The IHC/ISH analysis was able to distinguish immunophenotypic groups of GC with distinct characteristics and prognosis,resembling the subtypes of the molecular classifications.Accordingly,this method of classification may represent a viable option for use in a clinical setting. | Marcus Fernando Kodama Pertille Ramos Marina Alessandra Pereira Evandro Sobroza de Mello Cinthya dos Santos Cirqueira Bruno Zilberstein Venancio Avancini Ferreira Alves Ulysses Ribeiro-Junior Ivan Cecconello | 2021 | World Journal of Clinical Oncology2021，12，8： | 0 |
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