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| 1 | Transcription analysis of peloric mutants of Phalaenopsis orchids derived from tissue culture显示文摘Tissue culture has been widely used for mass propagation of Phalaenopsis. However, somaclonal variation occurred during micropropagation process posed a severe problem by affecting product quality. In this study, wild type and peloric flower buds of Phalaenopsis hybrids derived from flower stalk nodal culture were used for cDNA-RAPD and cDNA suppression subtractive hybridization analyses in order to study their genetic difference in terms of expressed sequence tags. A total of 209 ESTs from normal flower buds and 230 from mutants were sequenced. These ESTs sequences can be grouped into several functional categories involved in different cellular processes including metabolism, signal transduction, transcription, cell growth and division, protein synthesis, and protein localization, and into a subcat- egory of proteins with unknown function. Cymbidium mosaic virus transcript was surprisingly found expressed fre- quently in the peloric mutant of P. Little Mary. Real-time RT-PCR analysis on selected ESTs showed that in mutant flower buds, a bZIP transcription factor (TGA1a-like protein) was down-regulated, while up-regulated genes include auxin-regulated protein kinase, cyclophilin, and TCP-like genes. A retroelement clone was also preferentially expressed in the peloric mutant flowers. On the other hand, ESTs involved in DNA methylation, chromatin remodeling and post- transcriptional regulation, such as DNA methyltransferase, histone acetyltransferase, ERECTA, and DEAD/DEAH RNA helicase, were enriched in normal flower buds than the mutants. The enriched transcripts in the wild type indicate the down regulation of these transcripts in the mutants, and vice versa. The potential roles of the analyzed transcripts in the development of Phalaenopsis flowers are discussed. | Ya Huei CHEN Yi Jung TSAI Jian Zhi HUANG Fure Chyi CHEN | 2005 | Cell Research2005,15,8: | 21 |
| 2 | Species delimitation within the Glaucidium brodiei owlet complex using bioacoustic tools显示文摘Background:The taxonomy of the Collared Owlet(Glaucidium brodiei)species complex is confused owing to great individual variation in plumage colouration seemingly unrelated to their distribution.Although generally recognised as a single species,vocal differences among the subspecies have been noted by field recordists.However,there is no study assessing the vocal differences among these four subspecies.Methods:We obtained 76 sound recordings of the G.brodiei species complex comprising all four subspecies.We conducted bioacoustic examinations using principal component analysis and the Isler criterion to quantitatively test species boundaries within the G.brodiei complex.In addition,we compared plumage colouration among 13 specimens of the G.brodiei complex deposited at the Natural History Museum at Tring,UK and the Lee Kong China Natural History Museum,Singapore to ascertain the presence of plumage differences across taxa.Results:We found the Bornean and Sumatran populations vocally similar to each other,but distinctly different from the mainland and Taiwan populations.The vocal pattern seems to corroborate plumage distinctions in the colouration of neck collars:the Bornean and Sumatran taxa share a white neck collar,whereas the continental and Taiwan taxa share a rufous neck collar.Conclusions:We propose the taxonomic elevation of the Sumatran and Bornean populations to species level as Sunda Owlet G.sylvaticum,with one subspecies on Sumatra(G.s.sylvaticum)and Borneo(G.s.borneense)each.Our study corroborates the importance of bioacoustics in ascertaining species boundaries in non-passerines,and emphasises the significance of incorporating multiple species delimitation approaches when making taxonomic decisions. | Chyi Yin Gwee James AEaton Elize YXNg Frank ERheindt | 2019 | Avian Research2019,10,4: | 3 |
| 3 | 黑麂和费氏麂四种卫星DNA的克隆特征和染色体定位(英文)显示文摘近年来,分子细胞遗传学研究已基本证实了染色体的串联融合(端粒-着丝粒融合)是麂属动物核型演化的主要重排方式。尽管染色体串联融合的分子机制还不清楚,但通过染色体的非同源重组,着丝粒区域的卫星DNA被认为可能介导了染色体的融合。以前的研究发现在赤麂和小麂染色体的大部分假定的串联融合位点处存在着非随机分布的卫星DNA。然而在麂属的其他物种中,这些卫星DNA的组成以及在基因组中的分布情况尚未被研究。本研究从黑麂和费氏麂基因组中成功地克隆了4种卫星DNA(BMC5、BM700、BM1.1k和FM700),并分析了这些卫星克隆的特征以及在小麂、黑麂、贡山麂和费氏麂染色体上的定位情况。结果表明,卫星I和IIDNA(BMC5,BM700和FM700)的信号除了分布在这些麂属动物染色体的着丝粒区域外,也间隔地分布在这些物种的染色体臂上。其研究结果为黑麂、费氏麂和贡山麂的染色体核型也是从一个2n=70的共同祖先核型通过一系列的串联融合进化而来的假说提供了直接的证据。 | 刘妍 佴文惠 黄玲 王金焕 苏伟婷 LIN Chyi Chyang 杨凤堂 | 2008 | Zoological Research2008,29,3: | 2 |
| 4 | Resectoscopic treatment combined with sonohysterographic evaluation of women with postmenstrual bleeding as a result of previous cesarean delivery scar defects显示文摘 | Yu Chang Eing Mei Tsai Cheng Yu Long Chyi Long Lee Nari Kay | 2009 | American Journal of Obstetrics and Gynecology2009,,4: | 2 |
| 5 | Wave-Breaking Features of Blocking over Central Siberia and Its Impacts on the Precipitation Trend over Southeastern Lake Baikal显示文摘Precipitation over southeastern Lake Baikal features a significant decreasing trend in July and August over 1979–2018 and is closely related to blocking occurrence over central Siberia(45°–70°N,75°–115°E).This study investigates the formation and maintenance of anticyclonic and cyclonic wave-breaking(AWB and CWB)blocking events and their climate impacts on precipitation in the southeastern Lake Baikal area.Both AWB and CWB blocking events are characterized by a cold trough deepening from the sub-Arctic region and a ridge amplifying toward its north over central Siberia,as well as an evident Rossby wave train over midlatitude Eurasia.For AWB blocking events,the ridge and trough pair tilts clockwise and the wave train exhibits a zonal distribution.In contrast,ridge and trough pair associated with CWB blocking events leans anticlockwise with larger-scale,meridional,and more anisotropic signatures.Moreover,the incoming Rossby wave energy associated with CWB blocking events is more evident than for AWB blocking events.Therefore,CWB blocking events are more persistent.AWB blocking events produce more extensive and persistent precipitation over the southeastern Lake Baikal area than CWB blocking events,in which moderate above-normal rainfall is seen in the decaying periods of blockings.A significant decreasing trend is found in terms of AWB blocking occurrence over central Siberia,which may contribute to the downward trend of precipitation over southeastern Lake Baikal. | Dorina CHYI Zuowei XIE Ning SHI Pinwen GUO Huijun WANG | 2020 | Advances in Atmospheric Sciences2020,37,1: | 2 |
| 6 | Willingness to Pay for Online News: An Empirical Study on the Viability of the Subscription Model显示文摘 | Hsiang Iris Chyi | 2005 | Journal of Media Economics2005,,2: | 1 |
| 7 | Enhancement of the global convergence of using iterative dynamic programming to solve optimal control problems显示文摘 | LIN JengShiaw HWANG Chyi | 1998 | Indu & Eng Chem Res1998,37,6: | 1 |
| 8 | 显示文摘 | YIH- LUAN CHYI YEE- MAN LAI WEB- HSIEN LIU | 2012 | Research Policy2012,,41: | 1 |
| 9 | Variations of soil radon and thoron concentrations in a fault zone and prospective earthquakes in SW Taiwan显示文摘 | Yang T F Walia V Chyi L L | 2005 | Radiation Measurements2005,40,: | 1 |
| 10 | Indrajit ray chaudhary显示文摘 | XIAO HU SHAW CHYI TAN | 1997 | Journal of Materials Processing Technology1997,67,: | 1 |
| 11 | The effects of tuition reforms on school enrollment in rural China显示文摘 | Chyi H Zhou B | 2014 | Economics of Education Review2014,38,2: | 1 |
| 12 | Accurate estimation of surface roughness from texture features of the surface image using an adaptive neurofuzzy inference system 显示文摘 | Lee Kuang- chyi Hoa Shinn- jang Hob Shinn- ying | 2005 | Precision Engineering2005,,29: | 1 |
| 13 | 查看详情显示文摘 | Kishino K Unlti M S Chyi J I Reed J Arsenault L Morkoc H | | 0,,: | 1 |
| 14 | Amplification of DNA markers from evolutionarily diverse genomes using single primers of simple-sequence repeats显示文摘 | Gupta M Chyi Y S Romero-Severson J | 1994 | Theor Appl Genet1994,89,: | 1 |
| 15 | Reaction mechanism of isomaltooligosaccharides synthesis by alpha-glucosidase from Aspergillus carbonarious显示文摘 | Kow Jen Duan Dey Chyi Sheu Ming Tse Lin | 1994 | Biotechnology Letters1994,16,11: | 1 |
| 16 | Manganiferous cherts of theFranciscan assemblage, General geology, ancient and modem analogues, and implications for hydrothermal convection at oceanic spreadingcenters显示文摘 | Crerar D A Namson J Chyi M S | 1982 | Economic Geology1982,77,3: | 1 |
| 17 | Knowledge value chain显示文摘 | LEE CHING CHYI YANG JIE | 2000 | Journal of Management Development2000,19,9: | 1 |
| 18 | The distribution of gold and platinum in bituminous coal 显示文摘 | CHYI L L | 1982 | Econ Geol1982,77,6: | 1 |
| 19 | School outcomes of late preterm infants; special needs and challenges for infants born at 32 to 36 weeks gestation 显示文摘 | Chyi LJ Lee HC Hintz SR | 2008 | J Pediatr2008,153,: | 1 |
| 20 | A new approach to mixed H2/H∞ optimal PI/PID controller design显示文摘 | Hwang Chyi Hsiao Chun-Yen | 2002 | Ind Eng Chem Res2002,41,: | 1 |