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| 1 | Fucoidan enhances intestinal barrier function by upregulating the expression of claudin-1显示文摘AIM:To evaluate the protective effects of fucoidan on oxidative stress-induced barrier disruption in human intestinal epithelial cells.METHODS:In Caco-2 cell monolayer models,the disruption of barrier function by oxidative stress is mediated by H2O2.The integrity of polarized Caco-2 cell monolayers was determined by measuring the transepithelial resistance(TER)and permeability was estimated by measuring the paracellular transport of FITC-labeled4-kDa dextran(FD4).The protective effects of fucoidan on epithelial barrier functions on polarized Caco-2 cell monolayers were evaluated by TER and FD4 flux.The expression of tight junction(TJ)proteins was assessed using reverse-transcription polymerase chain reaction(RT-PCR)and immunofluorescence staining.RESULTS:Without H2O2treatment,fucoidan significantly increased the TER compared to control(P<0.05),indicating a direct enhancement of intestinal epithelial barrier function.Next,H2O2disrupted the epithelial barrier function in a time-dependent manner.Fucoidan prevented the H2O2-induced destruction in a dosedependent manner.Fucoidan significantly decreased H2O2-induced FD4 flux(P<0.01),indicating the prevention of disruption in paracellular permeability.RTPCR showed that Caco-2 cells endogenously expressed claudin-1 and-2,and occludin and that H2O2reduced the mRNA expression of these TJ proteins.Treatment with fucoidan attenuated the reduction in the expressions of claudin-1 and claudin-2 but not occludin.Immunofluorescence staining revealed that the expression of claudin-1 was intact and high on the cell surface.H2O2disrupted the integrity of claudin-1.Treatment with fucoidan dramatically attenuated the expression of claudin-1.CONCLUSION:Fucoidan enhanced intestinal epithelial barrier function by upregulating the expression of claudin-1.Thus,fucoidan may be an appropriate therapy for the treatment of inflammatory bowel diseases. | Atsushi Iraha Hiroshi Chinen Akira Hokama Takumi Yonashiro Tetsu Kinjo Kazuto Kishimoto Manabu Nakamoto Tetsuo Hirata Nagisa Kinjo Futoshi Higa Masao Tateyama Fukunori Kinjo Jiro Fujita | 2013 | World Journal of Gastroenterology2013，19，33： | 15 |
| 2 | Comparison of endoscopic stenting for malignant biliary obstruction: A single-center study显示文摘AIM: To evaluate the efficacy and safety of single-step endoscopic placement of self-expandable metallic stents(SEMS) for treatment of obstructive jaundice.METHODS: A retrospective study was performed among 90 patients who underwent transpapillary biliary metallic stent placement for malignant biliary obstruction(MBO) between April 2005 and October 2012. The diagnosis of primary disease and MBO was based on abdominal ultrasound, computed tomography, magnetic resonance imaging, endoscopic ultrasound, endoscopic retrograde cholangiopancreatography with brush cytology, biopsy, and/or a combination of these modalities. The type of SEMS(covered or non-covered, 8 mm or 10 mm in diameter) was determined by the endoscopist. Ninety patients were divided into two groups: group 1(49 patients) who underwent a singlestep SEMS placement and group 2(41 patients) who underwent a two-step SEMS placement. The technical success rate, complication rate, stent patency, and patient survival rate were compared between the groups. In addition, to identify the clinical prognostic factors associated with patient survival, the following variables were evaluated in Cox-regression analysis: gender, age, etiology of MBO(pancreatic cancer or nonpancreatic cancer), clinical stage(Ⅳb; with distant metastases or Ⅳa >; without distant metastases), chemotherapy(with or without), patency of the stent, and the use of single-step or two-step SEMS. RESULTS: Immediate technical success was achieved in 93.9%(46/49) in group 1 and in 95.1%(39/41) in group 2, with no significant difference(P = 1.0). Similarly, there was no difference in the complication rates between the groups(group 1, 4.1% and group 2, 4.9%; P = 0.62). Stent failure was observed in 10 cases in group 1(20.4%) and in 16 cases in group 2(39.0%). The patency of stent and patient survival revealed no difference between the two groups with Kaplan-Meier analysis, with a mean patency of 111 ± 17 d in group 1 and 137 ± 19 d in group 2(P = 0.91), and a mean survival of 178 ± 35 d in group 1 and 222 ± 23 d in group 2(P = 0.57). On the contrary, the number of days of hospitalization associated with first-time SEMS placement in group 1 was shorter when compared with that number in group 2(28 vs 39 d; P < 0.05). Multivariate analysis revealed that a clinical stage of Ⅳa >(P = 0.0055), chemotherapy(P = 0.0048), and no patency of the stent(P = 0.011) were independent prognostic factors associated with patient survival.CONCLUSION: Our results showed that single-step endoscopic metal stent placement was safe and effective for treating obstructive jaundice secondary to various inoperable malignancies. | Ryuichi Yamamoto Masatomo Takahashi Yasuyo Osafune Katsuya Chinen Shingo Kato Sumiko Nagoshi Koji Yakabi | 2015 | World Journal of Gastrointestinal Endoscopy2015，7，9： | 12 |
| 3 | Diagnostic utility of endoscopic ultrasound-guided fineneedle aspiration biopsy for glomus tumor of the stomach显示文摘A 52-year-old man was referred for further investigation of a gastric submucosal tumor on the greater curvature of the antrum. Endoscopic ultrasonography demonstrated a hypoechoic solid mass, which was primarily connected to the muscular layer of the stomach. We performed endoscopic ultrasoundguided fine-needle aspiration biopsy. The pathological examination showed proliferation of oval-shaped cells with nest formation, which stained strongly positive for muscle actin, and negative for c-kit, CD34, CD56,desmin, S-100, chromogranin, and neuron-specific enolase. Therefore, we performed laparoscopy and endoscopy cooperative surgery based on the preoperative diagnosis of glomus tumor of the stomach. The final histological diagnosis confirmed the preoperative diagnosis. Although preoperative diagnosis of glomus tumor of the stomach is difficult with conventional images and endoscopic biopsy, endoscopic ultrasoundguided fine-needle aspiration biopsy is an essential tool to gain histological evidence of glomus tumor of the stomach for early diagnosis. | Shin Kato Kaoru Kikuchi Kenji Chinen Takahiro Murakami Fumihito Kunishima | 2015 | World Journal of Gastroenterology2015，21，22： | 7 |
| 4 | The influence of transforming growth factor‐α, cyclooxygenase‐2, matrix metalloproteinase (MMP)‐7, MMP‐9 and CXCR4 proteins involved in epithelial–mesenchymal transition on overall survival of patients with gastric cancer显示文摘 | Marcello Ferretti Fanelli Ludmilla T Domingos Chinen Maria Dirlei Begnami Wilson Luis Costa José Humberto T Fregnami Fernando A Soares André Luis Montagnini | 2012 | Histopathology2012，，2： | 2 |
| 5 | A novel mutation (N32K) of GLUT2 gene in a Japanese patient with Fanconi - Bickel syndrome 显示文摘 | Matsuura T T Tamura Y Chinen | 2002 | Clin Genet2002，62，3： | 1 |
| 6 | Scripta Materialia显示文摘 | Hibiki Chinen | 2007 | 56:1412007，56，： | 1 |
| 7 | Meningeal earcinomatosis in solid tumors显示文摘 | Martins S J Azevedo CR Chinen LT | 2011 | Arq Neuropsiquiatr2011，69，6： | 1 |
| 8 | JPEDAV显示文摘 | Hibiki Chinen | 2009 | 30(6): 5872009，30，6： | 1 |
| 9 | Meningeal carcinomatosis in solid tumors显示文摘 | Martins SJ Azevedo CR Chinen LT | | 0，，： | 1 |
| 10 | Vascular lipotoxicity : endothelial dysfunction via fatty-acid-induced reactive oxygen species over production in obese Zucker diabetic fatty rats 显示文摘 | Chinen I Shimabukuro M Yamakawa K | 2007 | Endocrinology2007，148，1： | 1 |
| 11 | Outcomes of patients with severe combined immunodeficiency trcated with hematopoietie stem cell transplantation with and without preconditioning显示文摘 | Patel NC Chinen J Rosenblatt HM | 2009 | J Allergy Clin Immunol2009，124，5： | 1 |
| 12 | Multiple sclerosis: autoim- mune disease 显示文摘 | CHINEN A YOKOYAMA M | 1971 | Hawaii Med J1971，30，： | 1 |
| 13 | 2016年基础及临床免疫学进展显示文摘2016年基础免疫学研究进展包括进一步阐明了不同蛋白成分在不同效应T细胞和效应B细胞中所起的作用,如肌动蛋白细胞骨架中涉及的细胞因子和蛋白质。通过对家族性噬血细胞综合征的研究进一步阐明了细胞毒性细胞中颗粒物质形成和分泌的规律。确定了甲羟戊酸激酶缺乏导致的炎症反应的患者中异戊烯化的作用。同时也回顾了临床免疫研究进展,以及全基因组测序新方法和新报道的与免疫缺陷相关基因,如T细胞活化连接蛋白基因(LAT); B细胞淋巴瘤/白血病相关基因11B (BCL11B);具有RGD序列、富含亮氨酸重复序列、原肌球蛋白调节蛋白结构域、富含脯氨酸结构域的蛋白(RLTPR);膜突蛋白; JAK激酶1 (JAK1)。在原发性免疫缺陷进行造血干细胞移植和基因治疗方面取得相对意义上的成功;证实了自体病毒特异性细胞毒性T细胞使用的有效性。正在探索新的药物用法,例如吡格列酮在慢性肉芽肿患者中对增强呼吸爆发方面所起的作用。在HIV感染疫苗研究方面的持续进展为抗高突变率病毒免疫应答提供思路。 | Javier Chinen Yousef R.Badran Raif S.Geha Janet S.Chou Ari J.Fried 李俊达(翻译) 孙英(审校) | 2018 | 中华临床免疫和变态反应杂志2018，12，2： | 1 |
| 14 | Effects of pore size onseparation mechanisms of microfiltration of oily water, using porousglass tubular membrane 显示文摘 | OHYA H KIM J J CHINEN A | 1998 | J Membr Sci1998，145，： | 1 |
| 15 | Involvement of the spliceosomal U4 small nuclear RNA in heterochromatic gene silencing at fission yeast centromeres显示文摘 | Chinen M Morita M Fukumura K | 2010 | J Biol Chem2010，285，8： | 1 |
| 16 | Trichosporon pullulans infection in patients with chronic granulomatous disease:an emerging pathogen and review of the literature显示文摘 | Moylett EH Chinen J Shearer WT | 2003 | J Allergy Clin Immunol2003，111，6： | 1 |
| 17 | Studies on isoflavones and their attracting activity to Aphanomyces euteiches zoospore显示文摘 | Sekizaki H Yokosawa R Chinen C | 1993 | Biol Pharm Bull1993，16，7： | 1 |
| 18 | Improved agar diffusion method for detecting residual antimicrobial agents显示文摘 | Chinen Tsai Fusao Kondo | 2001 | Journal of food protection2001，64，3： | 1 |
| 19 | Vascular lipotoxicity: endothelial dysfunction via fatty-acid-induced reactive oxygen species overproduction in obese Zucker di- abetic fatty rats显示文摘 | Chinen I Shimabukuro M Yamakawa K | 2007 | Endocrinology2007，148，： | 1 |
| 20 | Suppressor of cytokine signaling-1 regulates inflammatory bowel dis- ease in which both IFNgamma and IL-4 are involved 显示文摘 | Chinen T Kobayashi T Ogata H | 2006 | Gastroenterology2006，30，2： | 1 |
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