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| 1 | Effect of anti-fibrosis compound on collagen expression of hepatic cells in experimental liver fibrosis of rats显示文摘INTRODUCTIONLiver fibrosis is mainly characterized by theexcessive synthesis and decreased degradation ofextracellular matrix(ECM),especially the synthesisand deposition of collagen.Almost all kinds of cellsin the liver have participated in the production ofcollagen.The most important ones are hepaticstellate cells(HSC)and hepatocytes.We | Wang LT Zhang B Chen JJ | 2000 | World Journal of Gastroenterology2000，6，6： | 31 |
| 2 | Enhancement of insulin-producing cell differentiation from embryonic stem cells using pax4-nucleofection method显示文摘AIM: To enhance the differentiation of insulin producing cell (IPC) ability from embryonic stem (ES) cells in vitro. METHODS: Four-day embryoid body (EB)-formatted ES cells were dissociated as single cells for the followed plasmid DNA delivery. The use of NucleofectorTM electroporator (Amaxa biosystems, Germany) in combination with medium-contained G418 provided a high efficiency of gene delivery for advanced selection. Neucleofected cells were plated on the top of fibronectincoated Petri dishes. Addition of Ly294002 and raised the glucose in medium at 24 h before examination.The differentiation status of these cells was monitored by semi-quantitative PCR (SQ-PCR) detection of the expression of relative genes, such as oct-4, sox-17, foxa2, mixl1, pdx-1, insulin 1, glucagons and somatostatin. The percentage of IPC population on d 18 of the experiment was investigated by immunohistochemistry (IHC), and the content/secretion of insulin was estimated by ELISA assay. The mice with severe combined immunodeficiency disease (SCID) pretreated with streptozotocin (STZ) were used to eliminate plasma glucose restoration after pax4+ ES implantation. RESULTS: A high efficiency of gene delivery was demonstrated when neucleofection was used in the present study; approximately 70% cells showed DsRed expression 2 d after neucleofection. By selection of medium-contained G418, the percentage of DsRed expressing cells kept high till the end of study. The pancreatic differentiation seemed to be accelerated by pax4 nucleofection. When compared to the group of cells with mock control, foxa2, mixl1, pdx1, higher insulin and somatostatin levels were detected by SQ-PCR 4 d after nucleofection in the group of pax4 expressing plasmid delivery. Approximately 55% of neucleofected cells showed insulin expression 18 d after neucleofection, and only 18% of cells showed insulin expression in mock control. The disturbance was shown by nucleofected pax4 RNAi vector; only 8% of cells expressed insulin 18 d after nucleofection. A higher IPC population was also detected in the insulin content by ELISA assay, and the glucose dependency was demonstrated in insulin secretion level. In the animal model, improvement of average plasma glucose concentration was observed in the group of pax-4 expressed ES of SCID mice pretreated with STZ, but no significant difference was observed in the group of STZ-pretreated SCID mice who were transplanted ES with mock plasmid. CONCLUSION: Enhancement of IPC differentiation from EB-dissociated ES cells can be revealed by simply using pax4 expressing plasmid delivery. Not only more IPCs but also pancreatic differentiation-related genes can be detected by SQ-PCR. Expression of relative genes, such as foxa 2, mixl 1, pdx-1, insulin 1 and somatostatin after nucleofection, suggests that pax4 accelerates the whole differentiation progress. The higher insulin production with glucose dependent modulation suggests that pax4 expression can drive more mature IPCs. Although further determination of the entire mechanism is required, thepotential of pax-4-nucleofected cells in medical treatment is promising. | Han-Tso Lin Chung-Lan Kao Kun-Hsiung Lee Yuh-Lih Chang Shih-Hwa Chiou Fu-Ting Tsai Tung-Hu Tsai Dey-Chyi Sheu Larry LT Ho Hung-Hai Ku | 2007 | World Journal of Gastroenterology2007，13，11： | 10 |
| 3 | 急性脊髓损伤患者治疗的临床操作指南:关于MRI基线在临床治疗决策与结果预测中作用的几点建议(英文)显示文摘The objective of this guideline is to outline the role of magnetic resonance imaging(MRI) in clinical decision making and outcome prediction in patients with traumatic spinal cord injury(SCI).Methods A systematic review of the literature was conducted to address key questions related to the use of MRI in patients with traumatic SCI.This review focused on longitudinal studies that controlled for baseline neurologic status.A multidisciplinary Guideline Development Group(GDG) used this information,their clinical expertise,and patient input to develop recommendations on the use of MRI for SCI patients.Based on GRADE(Grading of Recommendation,Assessment,Development and Evaluation),a strong recommendation is worded as ' we recommend,' whereas a weaker recommendation is indicated by 'we suggest.' Results Based on the limited available evidence and the clinical expertise of the GDG,our recommendations were:(1) 'We suggest that MRI be performed in adult patients with acute SCI prior to surgical intervention,when feasible,to facilitate improved clinical decision-making'(quality of evidence,very low) and(2) 'We suggest that MRI should be performed in adult patients in the acute period following SCI,before or after surgical intervention,to improve prediction of neurologic outcome '(quality of evidence,low).Conclusions These guidelines should be implemented into clinical practice to improve outcomes and prognostication for patients with SCI. | Fehlings MG Martin AR Tetreault LA Aarabi B Anderson P Arnold PM Brodke D Burns AS Chiba K Dettori JR Furlan JC Hawryluk G Holly LT Howley S Jeji T Kalsi-Ryan S Kotter M Kurpad S Kwon BK Marino RJ Massicotte E Merli G Middleton JW Nakashima H Nagoshi N Palmieri K Singh A Skelly AC Tsai EC Vaccaro A Wilson JR Yee A Harrop JS | 2017 | 中华神经外科疾病研究杂志2017，16，6： | 8 |
| 4 | FOUR NEW GLYCOSIDES, LIGUROBUSTOSIDES A, B, C AND D FROM LIGUSTRUM ROBUSTUM显示文摘FOURNEWGLYCOSIDES,LIGUROBUSTOSIDESA,B,CANDDFROMLIGUSTRUMROBUSTUM￥JunTIAN;HongJieZHANGandHanDongSUN.(Laboratoryofphytochemistr... | Tian, J Zhang, HJ Sun, HD Pan, LT Yao, P Chen, DY | 1996 | Chinese Chemical Letters1996，7，4： | 3 |
| 5 | Awareness of eye complications and prevalence of retinopathy in the first visit to eye clinic among type 2 diabetic patients显示文摘AIM:To assess the awareness of eye complications and the prevalence of retinopathy,in the first visit to eye clinic,among type 2 diabetic patients attending a tertiary medical centre in Kuala Lumpur,Malaysia.METHODS:An investigator-administered questionnaire was given to 137 patients with diabetes undergoing first time eye screening in the eye clinic.This was followed by a detailed fundus examination by a senior ophthalmologist to assess for presence of retinopathy.RESULTS:Almost 86% of respondents were aware of diabetic eye complications,especially in patients who had achieved tertiary educational level(96.3%).The majority of the patients(78.8%) were referred by their physicians and only 20.4% came on their own initiative.Many of the patients(43.8%) did not know how frequent they should go for an eye check-up and 72.3% did not know what treatments were available.Lack of understanding on diabetic eye diseases(68.6%) was the main barrier for most patients for not coming for eye screening earlier.Despite a high level of awareness,only 21.9% had recorded HbA1c level of <6.5% while 31.4% were under the erroneous assumption of having a good blood sugar control.A total of 29.2% had diabetic retinopathy in their first visit eye testing.CONCLUSION:In the present study,29.2% of type 2 diabetic patients had retinopathy in their first time eye testing.Although the awareness of diabetic eye complications was high among first time eye screening patients,the appropriate eye care-seeking behavior was comparatively less and should be rectified to prevent the rise of this sight threatening eye disease. | I Tajunisah PS Wong LT Tan P Rokiah SC Reddy | 2011 | International Journal of Ophthalmology(English edition)2011，4，5： | 3 |
| 6 | 透析前后血压水平与全因死亡风险的关系:一项剂量-效应关系的荟萃分析显示文摘血液透析人群的血压管理是一个巨大挑战。该研究旨在通过剂量-效应关系的荟萃分析,评估血液透析人群中透析前后血压水平与全因死亡风险之间关联的定量特征和潜在阈值。研究人员搜索了2017年3月18日之前发表的、关于透析前后血压水平与全因死亡率风险关联的所有前瞻性队列研究资料,共纳入来自8项研究的229688例普通血液透析患者。 | 刘莉 叶鹏 Han YC Tu Y Zhou LT Pan MM Wang B Liu H Tang RN Liu BC | 2018 | 中华高血压杂志2018，26，11： | 2 |
| 7 | Astrocytes produced and released interleukin-1, interleukin-6,tumor necrosis factor alpha and interferongamma under traumatic and metabolic injuries显示文摘 | Lau LT Yu CH | 2001 | J Neurotrauma2001，18，： | 2 |
| 8 | Classification of subtype of acute ischemic显示文摘 | Adame H Bendixen BH Kapelle LT | 1993 | Stroke1993，24，1： | 2 |
| 9 | 含蜡原油流动特性在管道设计中的应用(下)显示文摘 | Ward.,LT 常大海 | 1992 | 国外油气储运1992，10，6： | 2 |
| 10 | Visual contrast response functions in Parkinson's disease:Evidence from electroretinograms, visually evoked potentials and psychophysics显示文摘 | Langheinrich T Elst LT Lagreze WA | 2000 | Clin Neurophysiol2000，111，： | 2 |
| 11 | Response phase of the flicker electroretinogram (ERG) is influenced by cone excitation strength显示文摘 | Usui T Kremers J Sharpe LT | 1998 | Vision Res1998，38，： | 2 |
| 12 | Focal adhesions and associated proteins in medullary thyroid carcinoma cells显示文摘 | Kim LT Fleming JB Lopez-Guzman C | 2003 | J Surg Res2003，111，2： | 2 |
| 13 | A new concept of the sympathetic pathways to the eye显示文摘 | Palumbo LT | 1976 | Ann Ophthalmol1976，8，8： | 1 |
| 14 | Role and mechanisms of interleukin-1 in the modulation of neurotoxicity 显示文摘 | Ma XC Gottschall PE Chen LT | 2003 | Neuroimmunomodulation2003，10，4： | 1 |
| 15 | Level and value of circulating endothelial progenitor cells in patients after acute ischemic stroke显示文摘 | Yip HK Chang LT Chang WN | 2008 | Stroke2008，39，1： | 1 |
| 16 | Escitalopram, the S ( + ) enantiomer of citalopram,is a selective serotonin reuptake inhibitor with potent effects in animal models predictive of antidepressant and anxiolytie activities 显示文摘 | Sanchez C Bergqvist PB Brennum LT | 2003 | Psychopharmacology ( Bed )2003，167，： | 1 |
| 17 | Preoperative assessment of corneal and refractive stability in soft contact lens wearing photorefractive candidates显示文摘 | Ng LT Lee EM Nguyen AL | 2007 | Optom Vis Sci2007，84，5： | 1 |
| 18 | Timedependent behavior of interstitial fluid pressure in solid tumors : implications for drug delivery显示文摘 | Netti PA Baxter LT Boucher Y | 1995 | Cancer Research1995，55，： | 1 |
| 19 | Increasing prevalence of gastroschisia: population-based study in California 显示文摘 | Vu LT Nobuhara KK Laurent C | 2008 | J Pediatr2008，152，6： | 1 |
| 20 | Frequent somatic mutations and homozygous of the P16(MTS1) gene in pancreatic adenocarcinoma显示文摘 | Hahn SA da Costa LT | 1994 | Nat Genet1994，8，： | 1 |
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