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| 1 | Computed tomography-guided percutaneous core needle biopsy in pancreatic tumor diagnosis显示文摘AIM: To evaluate the techniques, results, and complications related to computed tomography(CT)-guided percutaneous core needle biopsies of solid pancreatic lesions.METHODS: CT-guided percutaneous biopsies of solid pancreatic lesions performed at a cancer reference center between January 2012 and September 2013 were retrospectively analyzed. Biopsy material was collected with a 16-20 G Tru-Core needle(10-15 cm; Angiotech, Vancouver, CA) using a coaxial system and automatic biopsy gun. When direct access to the lesion was not possible, indirect(transgastric or transhepatic) access or hydrodissection and/or pneumodissection maneuvers were used. Characteristics of the patients, lesions, procedures, and histologic results were recorded using a standardized form. RESULTS: A total of 103 procedures included in the study were performed on patients with a mean age of 64.8 year(range: 39-94 year). The mean size of the pancreatic lesions was 45.5 mm(range: 15-195 mm). Most(75/103, 72.8%) procedures were performed via direct access, though hydrodissection and/or pneumodissection were used in 22.2%(23/103) of cases and indirect transhepatic or transgastric access was used in 4.8%(5/103) of cases. Histologic analysis was performed on all biopsies, and diagnoses were conclusive in 98.1%(101/103) of cases, confirming3.9%(4/103) of tumors were benign and 94.2%(97/103) were malignant; results were atypical in 1.9%(2/103) of cases, requiring a repeat biopsy to diagnose a neuroendocrine tumor, and surgical resection to confirm a primary adenocarcinoma. Only mild/moderate complications were observed in 9/103 patients(8.7%),and they were more commonly associated with biopsies of lesions located in the head/uncinate process(n =8), than of those located in the body/tail(n = 1) of the pancreas, but this difference was not significant.CONCLUSION: CT-guided biopsy of a pancreatic lesion is a safe procedure with a high success rate, and is an excellent option for minimally invasive diagnosis. | Chiang J Tyng Maria Fernanda A Almeida Paula NV Barbosa Almir GV Bitencourt José Augusto AG Berg Macello S Maciel Felipe JF Coimbra Luiz Henrique O Schiavon Maria Dirlei Begnami Marcos D Guimares Charles E Zurstrassen Rubens Chojniak | 2015 | World Journal of Gastroenterology2015，21，12： | 14 |
| 2 | Nutritional evaluation of integral cassava root silages for growing pigs显示文摘The experiment aimed at determining the nutritional value of integral cassava root silages with yogurt as inoculant or wastewater(manipueira) by liquid addition ensiling process. Eighteen crossbred piglets(Large White x Landrace), castrated males with an initial average weight of 50 kg were allocated in metabolism cages throughout 11-day trial duration(6 days for animal's adaptation to the cages and to the experimental diets, and 5 days of urine and faeces collection). The experimental design was a randomized block arrangement with 3 treatments and 6 replicates: basal diet(100%); mixture composed of75% basal diet and 25% integral cassava root silage with yogurt as inoculant; a mixture composed of 75%of basal diet and 25% integral cassava root silage with wastewater. The silages with wastewater and yogurt presented the following values of apparent digestibility of dry matter 89.96% and 90.01%,apparent digestibility of crude protein of 60.67% and 66.43%, apparent digestibility of gross energy of90.43% and 91.48%, gross energy metabolizability coefficients of 87.88% and 88.93%, digestible energy values of 3,705 and 3,783 kcal/kg on a dry matter(DM) basis, and metabolizable energy values of 3,600 and 3,676 kcal/kg DM, respectively. The results have demonstrated that integral cassava root silages with wastewater or yogurt have a high nutritional value and can be used as an alternative energy source in growing pig's diets. | Diego D.Araújo Alessandro B.Amorim Mayra A.D.Saleh Felipe Curcelli Pedro L.Perdigón Silvio J.Bicudo Dirlei A.Berto | 2016 | Animal Nutrition2016，，3： | 5 |
| 3 | The influence of transforming growth factor‐α, cyclooxygenase‐2, matrix metalloproteinase (MMP)‐7, MMP‐9 and CXCR4 proteins involved in epithelial–mesenchymal transition on overall survival of patients with gastric cancer显示文摘 | Marcello Ferretti Fanelli Ludmilla T Domingos Chinen Maria Dirlei Begnami Wilson Luis Costa José Humberto T Fregnami Fernando A Soares André Luis Montagnini | 2012 | Histopathology2012，，2： | 2 |
| 4 | Digestible lysine levels in diets for pigs from 24 to 50 kg under sanitary segregation 显示文摘 | ERIKA ROSENDO DE SENA GANDRA MESSIAS ALVES DA TRINDADE NETO DIRLEI ANTONIO BERTO | 2012 | Revista Brasileira de Zootecnla2012，41，20： | 1 |
| 5 | The ultra-sonication of minerals in swine feed显示文摘A sample preparation method based on ultrasound assisted-extraction(UAE) of Ca,Mg and P from swine feed has been described.The experiment was performed to cover the variables influencing the sonication process and,the method validation using standard reference material.Final solutions obtained upon sonication were analyzed by flame atomic absorption spectrometry(for Ca and Mg) and by UV-vis spectrophotometry(for P).The best conditions for metal extraction were as follows:sample mass:100 mg in 20 ml_ 0.10 mol/L HCI,a particle size:<60 urn,sonication time:5 cycles of 10 s and ultrasound power:102 W.The UAE method was applied in digestibility assays in different piglet feeds and their results showed that it is highly comparable(P > 0.05) to the other methods used for such purposes,as block digestion,and offered a Ca,Mg and P method of quantification limit of 10.6,12.4 and 14 mg/kg,respectively.The major advantages of the UAE method compared to other methods are the high treatment rate,low reagent usage in the extracts and,it does not generate toxic residues that might negatively affect human health and the environment,accompanied by good precision and accuracy. | Mayra A.D.Saleh Pedro M.Padilha Lucelia Hauptli Dirlei A.Berto | 2015 | Journal of Animal Science and Biotechnology2015，6，4： | 1 |
| 6 | Sludge wash-out as strategy for anammox process start-up显示文摘 | Dirlei DK Valeria R Willibaldo S | 2007 | Process Biochemistry2007，42，： | 1 |
| 7 | Sludge wash-out as strategy for Anammox process start-up 显示文摘 | DIRLEI D K VALE 1t R WILLIBALDO S | 2007 | Process Biochemistry2007，42，12： | 1 |
| 8 | Dietary glutamine,glutamic acid and nucleotide supplementation accelerate carbon turnover(δ^(13)C)on stomach of weaned piglets显示文摘The use of stable isotope analysis as a tool for characterization of carbon turnover(δ^(13)C) in piglet's tissues by tracing its feeding system has drawn attention. Thus, this study aimed at evaluating the influence of dietary glutamine, glutamic acid and nucleotides supplementation on carbon turnover in fundic-stomach region of weaned piglets at an average age of 21 days. The diets consisted of additive-free diet — control(C); 1% glutamine(G); 1% glutamic acid(GA) and 1% nucleotides(Nu). At weaning day(day 0: baseline), 3 piglets were slaughtered to quantify the δ^(13)C of stomach. The remaining 120 piglets were blocked by weight and sex, randomly assigned to pens with 3 piglets slaughtered per treatment at days 1,2,4, 5,7,9,13.20.27 and 49 after weaning in order to verify the fundic-stomach isotopic composition by treatments.Samples were analyzed in terms of ^(13)C/^(12)C ratio by mass spectrometry and converted to relative isotopic enrichment values(δ^(13)C ‰) used to plot the first order exponential curves over time using OriginPro 8.0 software. The inclusion of glutamine, glutamate and nucleotides in piglet's diets has accelerated the carbon turnover in stomach during the post-weaning period, demonstrating also that glutamate has guaranteed fastest 13 C incorporation rate on fundic-stomach region and pH-lowering. Besides that, stable isotopes technique(δ^(13)C) has proved to be an important methodology to determine the time-scales at which piglets shift among diets with different isotopic values, characterizing the trophic effects of additives and the phenotypic flexibility of stomach. | Amanda D.Assoni Alessandro B.Amorim Mayra A.D.Saleh Marcos L.P.Tsea Dirlei A.Berto | 2017 | Animal Nutrition2017，，3： | 0 |
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