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| 1 | dentification of TRA2B-DNAH5 fusion as a novel oncogenic driver in human lung squamous cell carcinoma显示文摘 | Fei Li Zhaoyuan Fang Jian Zhang Chen Li Hongyan Liu Jufeng Xia Hongwen Zhu Chenchen auo Zhen Qin Fuming Li Xiangkun Han Yuetong Wang Yan Feng Ye Wang Wenjing Zhang Zuoyun Wang Yujuan Jin Yihua Sun Wenyi Wei Rong Zeng Haiquan Chen Hongbin Ji | 2016 | Cell Research2016，26，10： | 9 |
| 2 | PI3K/Akt/mTOR signaling orchestrates the phenotypic transition and chemo-resistance of small cell lung cancer显示文摘Small cell lung cancer(SCLC)is a phenotypically heterogeneous disease with an extremely poor prognosis,which is mainly attributed to the rapid development of resistance to chemotherapy.However,the relation between the growth phenotypes and chemo-resistance of SCLC remains largely unclear.Through comprehensive bioinformatic analyses,we found that the heterogeneity of SCLC phenotype was significantly associated with different sensitivity to chemotherapy.Adherent or semiadherent SCLC cells were enriched with activation of the PI3K/Akt/mTOR pathway and were highly chemoresistant.Mechanistically,activation of the PI3K/Akt/mTOR pathway promotes the phenotypic transition from suspension to adhesion growth pattern and confers SCLC cells with chemo-resistance.Such chemo-resistance could be largely overcome by combining chemotherapy with PI3K/Akt/mTOR pathway inhibitors.Our findings support that the PI3K/Akt/mTOR pathway plays an important role in SCLC phenotype transition and chemo-resistance,which holds important clinical implications for improving SCLC treatment. | Xuefeng Li Cheng Li Chenchen Guo Qiqi Zhao Jiayu Cao Hsin-Yi Huang Meiting Yue Yun Xue Yujuan Jin Liang Hu Hongbin Ji | 2021 | Journal of Genetics and Genomics2021，48，7： | 5 |
| 3 | The RNA-binding protein Musashi2 governs osteoblast-adipocyte lineage commitment by suppressing PPARγsignaling显示文摘Osteoporosis caused by aging is characterized by reduced bone mass and accumulated adipocytes in the bone marrow cavity. How the balance between osteoblastogenesis and adipogenesis from bone marrow mesenchymal stem cells(BMSCs) is lost upon aging is still unclear. Here, we found that the RNA-binding protein Musashi2(Msi2) regulates BMSC lineage commitment. Msi2 is commonly enriched in stem cells and tumor cells. We found that its expression was downregulated during adipogenic differentiation and upregulated during osteogenic differentiation of BMSCs. Msi2 knockout mice exhibited decreased bone mass with substantial accumulation of marrow adipocytes, similar to aging-induced osteoporosis. Depletion of Msi2 in BMSCs led to increased adipocyte commitment. Transcriptional profiling analysis revealed that Msi2 deficiency led to increased PPARγ signaling.RNA-interacting protein immunoprecipitation assays demonstrated that Msi2 could inhibit the translation of the key adipogenic factor Cebpα, thereby inhibiting PPAR signaling. Furthermore, the expression of Msi2 decreased significantly during the aging process of mice, indicating that decreased Msi2 function during aging contributes to abnormal accumulation of adipocytes in bone marrow and osteoporosis. Thus, our results provide a putative biochemical mechanism for aging-related osteoporosis, suggesting that modulating Msi2 function may benefit the treatment of bone aging. | Jinlong Suo Sihai Zou Jinghui Wang Yujiao Han Lingli Zhang Chenchen Lv Bo Jiang Qian Ren Long Chen Lele Yang Ping Ji Xianyou Zheng Ping Hu Weiguo Zou | 2022 | Bone Research2022，10，3： | 5 |
| 4 | Large chromosomal segment deletions by CRISPR/LbCpf1-mediated multiplex gene editing in soybean显示文摘The creation of new soybean varieties has been limited by genomic duplication and redundancy.Efficient multiplex gene editing and large chromosomal segment deletion through clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein(Cas)systems are promising strategies for overcoming these obstacles.CRISPR/Cpf1 is a robust tool for multiplex gene editing.However,large chromosomal excision mediated by CRISPR/Cpf1 has been reported in only a few non-plant species.Here,we report on CRISPR/LbCpf1-induced large chromosomal segment deletions in soybean using multiplex gene targeting.The CRISPR/LbCpf1 system was optimized for direct repeat and guide RNA lengths in crispr RNA(crRNA)array.The editing efficiency was evaluated using LbCpf1 driven by the CaMV35S and soybean ubiquitin promoter.The optimized system exhibited editing efficiencies of up to 91.7%.Our results showed eight gene targets could be edited simultaneously in one step when a single eight-gRNA-target crRNA array was employed,with an efficiency of up to 17.1%.We successfully employed CRISPR/LbCpf1 to produce small fragments(<1 Kb)and large chromosomal segment deletions(10 Kb-1 Mb)involving four different gene clusters in soybean.Together,these data demonstrate the power of the CRISPR/LbCpf1 platform for multiplex gene editing and chromosomal segment deletion in soybean,supporting the use of this technology in both basic research and agricultural applications. | Kaixuan Duan Yuanyuan Cheng Jing Ji Chenchen Wang Yongshu Wei Yuanchao Wang | 2021 | Journal of Integrative Plant Biology2021，63，9： | 5 |
| 5 | Association of systemic inflammation and body mass index with survival in patients with resectable gastric or gastroesophageal junction adenocarcinomas显示文摘Objective:The systemic inflammation index and body mass index(BMI)are easily accessible markers that can predict mortality.However,the prognostic value of the combined use of these two markers remains unclear.The goal of this study was therefore to evaluate the association of these markers with outcomes based on a large cohort of patients with gastric cancer.Methods:A total of 2,542 consecutive patients undergoing radical surgery for gastric or gastroesophageal junction adenocarcinoma between 2009 and 2014 were included.Systemic inflammation was quantified by the preoperative neutrophil-to-lymphocyte ratio(NLR).High systemic inflammation was defined as NLR≥3,and underweight was defined as BMI<18.5 kg/m2.Results:Among 2,542 patients,NLR≥3 and underweight were common[627(25%)and 349(14%),respectively].In the entire cohort,NLR≥3 or underweight independently predicted overall survival(OS)[hazard ratio(HR):1.236,95%confidence interval(95%CI):1.069–1.430;and HR:1.600,95%CI:1.350–1.897,respectively]and recurrence-free survival(RFS)(HR:1.230,95%CI:1.054–1.434;and HR:1.658,95%CI:1.389–1.979,respectively).Patients with both NLR≥3 and underweight(vs.neither)had much worse OS(HR:2.445,95%CI:1.853–3.225)and RFS(HR:2.405,95%CI:1.802–3.209).Furthermore,we observed similar results in subgroup analyses according to pathological stage,age,and postoperative chemotherapy.Conclusions:Our results showed that preoperative elevated NLR and decreased BMI had a significant negative effect on survival.Underweight combined with severe inflammation could enhance prognostication.Taking active therapeutic measures to reduce inflammation and increase nutrition may help improve outcomes. | Xianchun Gao Yanan Pan Weili Han Caie Hu Chenchen Wang Ling Chen Yong Guo Yupeng Shi Yan Pan Huahong Xie Liping Yao Jianjun Yang Jianyong Zheng Xiaohua Li Xiaonan Liu Liu Hong Jipeng Li Mengbin Li Gang Ji Zengshan Li Jielai Xia Qingchuan Zhao Daiming Fan Kaichun Wu Yongzhan Nie | 2021 | Cancer Biology & Medicine2021，18，1： | 2 |
| 6 | Amplification and expression of c-MET correlate with poor prognosis of patients with gastric cancer and upregulate the expression of PDL1显示文摘The prognostic significance of c-MET in gastric cancer(GC)remains uncertain.In the present study,we examined the amplification,expression,and the prognostic value of c-MET,human epidermal growth factor receptor 2(HER2),and programmed cell death 1 ligand 1(PDL1),together with the correlations among them in a large cohort of Chinese samples.A total of 444 patients were included.The immunohistochemistry(IHC)and the dual-color silver in situ hybridization(SISH)were performed to examine their expression and amplification.Univariate and multivariate analyses were performed by the Cox proportional hazard regression model,and survival curves were estimated by the Kaplan-Meier method.The positivity determined by IHC of c-MET was 24.8%,and the MET amplification rate was 2.3%.The positivity rates of HER2 and PDL1 were 8%and 34.7%,respectively.PDL1 expression had a significantly positive association with c-MET expression.c-MET positivity played a significant prognostic role in disease-free survival(DFS)(P=0.032).Patients with mesenchymal-epithelial transition(MET)amplification had significantly poorer prognosis on both DFS and overall survival(OS).Subgroup analysis showed that in HER2-negative patients,but not in HER2-positive patients,MET-positive patients had significantly worse DFS(P=0.000)and OS(P=0.006).c-MET regulated the expression of PDL1 through an AKT-dependent pathway.c-MET inhibitor enhanced the T-cell killing ability and increased the efficacy of PD1 antibody.c-MET was found to be an independent prognostic factor for DFS of GC patients.A combination of c-MET inhibitors and PD1 antibodies could enhance the killing capacity of T cells,providing a preliminary basis for the clinical research on the same combination in GC treatment. | Ya’nan Yang Chenchen Wang Congqi Dai Xinyang Liu Wenhua Li Mingzhu Huang Xiaoying Zhao Dongmei Ji Jin Li Weijian Guo | 2021 | Acta Biochimica et Biophysica Sinica2021，53，5： | 2 |
| 7 | Identification of TAZ as the essential molecular switch in orchestrating SCLC phenotypic transition and metastasis显示文摘Small-cell lung cancer(SCLC)is a recalcitrant cancer characterized by high metastasis.However,the exact cell type contributing to metastasis remains elusive.Using a Rb1^(L/L)/Trp53^(L/L) mouse model,we identify the NCAM^(hi)CD44^(lo/–) subpopulation as the SCLC metastasizing cell(SMC),which is progressively transitioned from the non-metastasizing NCAM^(lo)CD44^(hi) cell(non-SMC).Integrative chromatin accessibility and gene expression profiling studies reveal the important role of the SWI/SNF complex,and knockout of its central component,Brg1,significantly inhibits such phenotypic transition and metastasis.Mechanistically,TAZ is silenced by the SWI/SNF complex during SCLC malignant progression,and its knockdown promotes SMC transition and metastasis.Importantly,ectopic TAZ expression reversely drives SMC-to-non-SMC transition and alleviates metastasis.Single-cell RNA-sequencing analyses identify SMC as the dominant subpopulation in human SCLC metastasis,and immunostaining data show a positive correlation between TAZ and patient prognosis.These data uncover high SCLC plasticity and identify TAZ as the key molecular switch in orchestrating SCLC phenotypic transition and metastasis. | Yujuan Jin Qiqi Zhao Weikang Zhu Yan Feng Tian Xiao Peng Zhang Liyan Jiang Yingyong Hou Chenchen Guo Hsinyi Huang Yabin Chen Xinyuan Tong Jiayu Cao Fei Li Xueliang Zhu Jun Qin Dong Gao Xin-Yuan Liu Hua Zhang Luonan Chen Roman KThomas Kwok-Kin Wong Lei Zhang Yong Wang Liang Hu Hongbin Ji | 2022 | National Science Review2022，9，7： | 0 |
| 8 | An integrated portable bio-monitoring system based on tough hydrogels for comprehensive detection of physiological activities显示文摘Advanced soft ion-conducting hydrogels have been developed rapidly in the integrated portable health monitoring equipment due to their higher sensitivity,sensory traits,tunable conductivity,and stretchability for physiological activities and personal healthcare detection.However,traditional hydrogel conductors are normally susceptible to large deformation and strong mechanical stress,which leads to inferior electro-mechanical stability for real application scenarios.Herein,a strong ionically conductive hydrogel(poly(vinyl alcohol)-boric acid-glycerol/sodium alginate-calcium chloride/electrolyte ions(PBG/SC/EI))was designed by engineering the covalently and ionically crosslinked networks followed by the salting-out effect to further enhance the mechanical strength and ionic conductivity of the hydrogel.Owing to the collective effects of the energy-dissipation mechanism and salting-out effect,the designed PBG/SC/EI with excellent structural integrity and robustness exhibits exceptional mechanical properties(elongation at break for 559.1%and tensile strength of 869.4 kPa)and high ionic conductivity(1.618 S·m^(-1)).As such,the PBG/SC/EI strain sensor features high sensitivity(gauge factor=2.29),which can effectively monitor various kinds of human motions(joint motions,facial micro-expression,faint respiration,and voice recognition).Meanwhile,the hydrogel-based Zn||MnO_(2)battery delivers a high capacity of 267.2 mAh·g^(-1)and a maximal energy density of 356.8 Wh·kg^(-1)associated with good cycle performance of 71.8%capacity retention after 8000 cycles.Additionally,an integrated bio-monitoring system with the sensor and Zn||MnO_(2)battery can accurately identify diverse physiological activities in a real-time and non-invasive way.This work presents a feasible strategy for designing high-performance conductive hydrogels for highly-reliable integrated bio-monitoring systems with excellent practicability. | Congcong Yang Chenchen Ji Fengjiao Guo Chunjiang Jin Hongyu Mi Zhongchang Wang | 2024 | Nano Research2024，17，1： | 0 |
| 9 | Correction to:Identification of TAZ as the essential molecular switch in orchestrating SCLC phenotypic transition and metastasis显示文摘This is a correction to:Yujuan Jin,Qiqi Zhao,Weikang Zhu,Yan Feng,Tian Xiao,Peng Zhang,Liyan Jiang,Yingyong Hou,Chenchen Guo,Hsinyi Huang,Yabin Chen,Xinyuan Tong,Jiayu Cao,Fei Li,Xueliang Zhu,Jun Qin,Dong Gao,Xin-Yuan Liu,Hua Zhang,Luonan Chen,Roman K Thomas,Kwok-Kin Wong,Lei Zhang,Yong Wang,Liang Hu,Hongbin Ji,Identification of TAZ as the essential molecular switch in orchestrating SCLC phenotypic transition and metastasis,National Science Review,Volume 9,Issue 7,July 2022,nwab232,http://gffzzd3cc09b8251d45dfsx96cf90bonvc6vof.ffgz.tsg.suse.edu.cn/10.1093/nsr/nwab232. | Yujuan Jin Qiqi Zhao Weikang Zhu Yan Feng Tian Xiao Peng Zhang Liyan Jiang Yingyong Hou Chenchen Guo Hsinyi Huang Yabin Chen Xinyuan Tong Jiayu Cao Fei Li Xueliang Zhu Jun Qin Dong Gao Xin-Yuan Liu Hua Zhang Luonan Chen Roman KThomas Kwok-Kin Wong Lei Zhang Yong Wang Liang Hu Hongbin Ji | 2023 | National Science Review2023，10，12： | 0 |
| 10 | Polyanionic hydrogel electrolyte enables reversible and durable Zn anode for efficient Zn-based energy storage显示文摘Aqueous Zn-ion energy storage systems,which are expected to be integrated into intelligent electronics as a secure power supply,suffer poor reversibility of Zn anodes,predominantly associated with dendritic growth and side reactions.This study introduces a polyanionic strategy to address these formidable issues by developing a hydrogel electrolyte(PACXHE)with carboxyl groups.Notably,the carboxyl groups within the hydrogel structure establish favorable channels to promote the transport of Zn^(2+)ions.They also expedite the desolvation of hydrated Zn^(2+)ions,leading to enhanced deposition kinetics.Additionally,these functional groups confine interfacial planar diffusion and promote preferential deposition along the(002)plane of Zn,enabling a smooth surface texture of the Zn anode.This multifaceted regulation successfully achieves the suppression of Zn dendrites and side reactions,thereby enhancing the electrochemical reversibility and service life during plating/stripping cycles.Therefore,such an electrolyte demonstrates a high average Coulombic efficiency of 97.7%for 500 cycles in the Zn‖Cu cell and exceptional cyclability with a duration of 480 h at 1 mA cm^(-2)/1 mA h cm^(-2)in the Zn‖Zn cell.Beyond that,the Zn-ion hybrid micro-capacitor employing PACXHE exhibits satisfactory cycling stability,energy density,and practicality for energy storage in flexible,intelligent electronics.The present polyanionic-based hydrogel strategy and the development of PACXHE represent significant advancements in the design of hydrogel electrolytes,paving the way for a more sustainable and efficient future in the energy storage field. | Chunjiang Jin Congcong Yang Hongyu Mi Chenchen Ji Fengjiao Guo Chengzhe Liu Ziqiang Liu Nianjun Yang | 2023 | Journal of Energy Chemistry2023，，11： | 0 |
| 11 | Short-term prediction of on-street parking occupancy using multivariate variable based on deep learning显示文摘Short-term prediction of on-street parking occupancy is essential to the ITS system,which can guide drivers in finding vacant parking spaces.And the spatial dependencies and exogenous dependencies need to be considered simultaneously,which makes short-term prediction of on-street parking occupancy challenging.Therefore,this paper proposes a deep learning model for predicting block-level parking occupancy.First,the importance of multiple points of interest(POI)in different buffers is sorted by Boruta,used for feature selection.The results show that different types of POI data should consider different buffer radii.Then based on the real on-street parking data,long short-term memory(LSTM)that can address the time dependencies is applied to predict the parking occupancy.The results demonstrate that LSTM considering POI data after Boruta selection(LSTM(+BORUTA))outperforms other baseline methods,including LSTM,with an average testing MAPE of 11.78%.The selection process of POI data helps LSTM reduce training time and slightly improve the prediction performance,which indicates that complex correlations among the same type of POI data in different buffer zones will also affect the prediction accuracy of LSTM.When there are more restaurants on both sides of the street,the prediction performance of LSTM(+BORUTA)is significantly better than that of LSTM. | Mengqi Lyu Yanjie Ji Chenchen Kuai Shuichao Zhang | 2024 | Journal of Traffic and Transportation Engineering(English Edition)2024，11，1： | 0 |
| 12 | Distribution and Functional Characteristics of Voltage-Gated Sodium Channels in Immature Cochlear Hair Cells显示文摘Voltage-gated sodium channels(VGSCs)are transiently expressed in cochlear hair cells before hearing onset and play an indispensable role in shaping spontaneous activity.In this study,we showed that Na^+currents shaped the spontaneous action potentials in developing mouse inner hair cells(IHCs)by decreasing the time required for the membrane potential to reach the action-potential threshold.In immature IHCs,we identified 9 known VGSC subtypes(Navl.la-l.9ot),among which Navl.7a was the most highly expressed subtype and the main contributor to Na+currents in developing hair cells.Electrophysiological recordings of two cochlea-specific Navi.7 variants(CbmNavl.7a and CbmNavl.7b)revealed a novel loss-of-function mutation(C934R)at the extracellular linker between segments 5 and 6 of domain II.In addition,post-transcriptional modification events,such as alternative splicing and RNA editing,amended the gating properties and kinetic features of CbmNavl.7a(C934).These results provide molecular and functional characteristics of VGSCs in mammalian IHCs and their contributions to spontaneous physiological activity during cochlear maturation. | You Zhou Chenchen Xia Manli Yi Xueling Wang Hao Wu Yonghua Ji | 2020 | Neuroscience Bulletin2020，36，1： | 0 |
| 13 | DHA induces adipocyte lipolysis through endoplasmic reticulum stress and the cAMP/PKA signaling pathway in grass carp(Ctenopharyngodon idella)显示文摘Docosahexaenoic acid(DHA)is a biologically active fatty acid that reduces the accumulation of lipids.However,the molecular mechanism underlying this process,particularly in fish,is not well understood.Recent studies show that endoplasmic reticulum(ER)stress triggers the activation of the unfolded protein response,which has been revealed to play an essential role in lipid metabolism.In this study,we explored the effect of DHA on ER stress and investigated the potential molecular mechanisms underlying DHA-induced adipocyte lipolysis in grass carp(Ctenopharyngodon idella)both in vivo and in vitro.We found that DHA remarkably reduced the triglyceride content,increased the secretion of glycerol,pro-moted lipolysis in adipocytes and evoked ER stress,whereas inhibiting ER stress using 4-phenyl butyric acid(4-PBA)inhibited the effects of DHA(P<0.05).These results implied that ER stress potentially participates in DHA-induced adipocyte lipolysis.Additionally,STF-083010,a specific inositol-requiring enzyme 1a(IRE1a)-inhibitor,attenuated the effects of DHA on lipolysis,demonstrating that IRE1a and X-box binding protein 1 potentially participate in DHA-induced lipolysis.DHA also activated the cyclic adenosine monophosphate(cAMP)-dependent protein kinase A(PKA)pathway by increasing the level of cAMP and activating the PKA enzyme(P<0.05).Nevertheless,H89,a PKA inhibitor,weakened DHA-induced lipolysis by inhibiting the cAMP/PKA signaling pathway.Furthermore,inhibiting ER stress us-ing 4-PBA also inhibited lipolysis and alleviated DHA-induced activation of the cAMP/PKA signaling pathway,suggesting that ER stress may participate in DHA-induced lipolysis through the activation of the cAMP/PKA signaling pathway.Our data illustrate that DHA supplementation can be a promising nutritional strategy for ameliorating lipid accumulation in grass carp.The present study elucidated the molecular mechanism for DHA-induced lipolysis in grass carp adipocytes and emphasized the impor-tance of ER stress and the cAMP/PKA pathway in DHA-induced lipolysis.These results deepen our un-derstanding of ameliorating lipids deposition in freshwater fish by targeting DHA. | Xiaocheng Huang Chenchen Bian Hong Ji Shanghong Ji Jian Sun | 2023 | Animal Nutrition2023，，2： | 0 |
| 14 | Image Reconstruction for ECT under Compressed Sensing Framework Based on an Overcomplete Dictionary显示文摘Electrical capacitance tomography(ECT)has great application potential inmultiphase processmonitoring,and its visualization results are of great significance for studying the changes in two-phase flow in closed environments.In this paper,compressed sensing(CS)theory based on dictionary learning is introduced to the inverse problem of ECT,and the K-SVD algorithm is used to learn the overcomplete dictionary to establish a nonlinear mapping between observed capacitance and sparse space.Because the trained overcomplete dictionary has the property to match few features of interest in the reconstructed image of ECT,it is not necessary to rely on the sparsity of coefficient vector to solve the nonlinear mapping as most algorithms based on CS theory.Two-phase flow distribution in a cylindrical pipe was modeled and simulated,and three variations without sparse constraint based on Landweber,Tikhonov,and Newton-Raphson algorithms were used to rapidly reconstruct a 2-D image. | Xuebin Qin Yutong Shen Jiachen Hu Mingqiao Li Peijiao Yang Chenchen Ji Xinlong Zhu | 2022 | Computer Modeling in Engineering & Sciences2022，，3： | 0 |
| 15 | Palladium-Catalyzed Intramolecular Dehydrogenative Arylboration of Alkenes显示文摘The palladium-catalyzed borylative cyclization via C—H activation has been developed.By this chemistry,the indole-fused dihydro-pyrrole motif,which is a kind of important unit in natural products and bio-active molecules,could be constructed and installed with a boric ester group.Furthermore,the utilities of products have been illustrated by the study of further transformations.Importantly,by using chiral ligand,the enantioselectivity of this borylative cyclization reaction could be controlled.Moreover,the borylative mechanism,which should proceed through a Pd(II)/Pd(IV)catalytic cycle,has been proposed based on the DFT calculations. | Chenchen Zou Hongli Wu Yuqi Ji Penglin Zhang Huanhuan Cui Genping Huang Chun Zhang | 2022 | Chinese Journal of Chemistry2022，40，20： | 0 |
| 16 | Understanding SCLC heterogeneity and plasticity in cancer metastasis and chemotherapy resistance显示文摘Small cell lung cancer(SCLC)accounts for approximately 15%of all lung cancer cases and features a strong predilection for early metastasis and extremely poor prognosis.Despite being highly sensitive to chemotherapy and/or radiotherapy initially,most SCLC patients develop therapeutic resistance within one year and die of distant metastases.Multiple studies have revealed the high heterogeneity and strong plasticity of SCLC associated with frequent metastases and early development of therapeutic resistance as well as poor clinical outcome.Importantly,different SCLC subtypes are associated with different therapeutic vulnerabilities,and the inflamed subtype tends to have the best response to immunotherapy,which highlights the importance of precision medicine in the clinic.Here,we review recent advances in ScLc heterogeneity and plasticity and their link to distant metastases and chemotherapy resistance.We hope that a better understanding of the molecular mechanisms underlying SCLC malignant progression will help to develop better intervention strategies for this deadly disease. | Yujuan Jin Yanxu Chen Zhen Qin Liang Hu Chenchen Guo Hongbin Ji | 2023 | Acta Biochimica et Biophysica Sinica2023，55，6： | 0 |
| 17 | A mesenchymal-like subpopulation in non-neuroendocrine SCLC contributes to metastasis显示文摘Small cell lung cancer(SCLC)is the most aggressive lung cancer with high heterogeneity.Mouse SCLC cells derived from the Rb1L/L/Trp53L/L(RP)autochthonous mouse model grew as adhesion or suspension in cell culture,and the adhesion cells are defined as non-neuroendocrine(non-NE)SCLC cells.Here,we uncover the heterogenous subpopulations within the non-NE cells and referred to them as mesenchymallike(Mes)and epithelial-like(Epi)SCLC cells.The Mes cells have increased capability to form colonies in soft agar and harbored stronger metastatic capability in vivo when compared with the Epi cells.Gene Set Enrichment Analysis reveals that the transforming growth factor(TGF)-βsignaling is enriched in the Mes cells.Importantly,inhibition of the TGF-βsignaling through ectopic expression of dominant-negative Tgfbr2(Tgfbr2-DN)or treatment with Tgfbr1 inhibitor SD-208 consistently abrogates tumor metastasis in nude mouse allograft assays.Moreover,genetic deletion of Tgfbr2 or Smad4,the key components of the TGF-βsignaling pathway,dramatically attenuates SCLC metastasis in the RP autochthonous mouse model.Collectively,our results uncover the high heterogeneity in non-NE SCLC cells and highlight an important role of TGF-βsignaling in promoting SCLC metastasis. | Yujuan Jin Tian Xiao Yan Feng Jinhua Yang Chenchen Guo Liang Hu Hongbin Ji | 2021 | Journal of Genetics and Genomics2021，48，7： | 0 |
| 18 | Dynamic Analysis and Parametric Optimization of Telescopic Tubular Mast Applied on Solar Sail显示文摘Large-scale solar sails can provide power to spacecraft for deep space exploration.A new type of telescopic tubular mast(TTM)driven by a bistable carbon fiber-reinforced polymer tube was designed in this study to solve the problem of contact between the sail membrane and the spacecraft under light pressure.Compared with the traditional TTM,it has a small size,light weight,high extension ratio,and simple structure.The anti-blossoming and self-unlocking structure of the proposed TTM was described.We aimed to simplify the TTM with a complex structure into a beam model with equal linear mass density,and the simulation results showed good consistency.The dynamic equation was derived based on the equivalent model,and the effects of different factors on the vibration characteristics of the TTM were analyzed.The performance parameters were optimized based on a multiobjective genetic algorithm,and prototype production and load experiments were conducted.The results show that the advantages of the new TTM can complete the deployment of large-scale solar sails,which is valuable for future deep space exploration. | Chenyang Ji Jinguo Liu Chenchen Wu Pengyuan Zhao Keli Chen | 2023 | Chinese Journal of Mechanical Engineering2023，36，2： | 0 |
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