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| 1 | 利用水稻功能基因SSR标记鉴定水稻种质资源显示文摘利用 16对水稻功能基因的SSR引物研究了 2 3份世界 5个国家不同来源的水稻种质资源的遗传多样性 ,共检测出 78个等位基因变异 ,每对引物可检测 2~ 10个等位基因变异 ,平均为 5 .2个 ,品种间遗传相似系数在0 .13~ 0 .6 4之间。UPGMA聚类分析表明 ,在相似系数 0 .13处可以将材料分为 2大类 ,来自巴西、日本和中国的粳稻全部聚为第Ⅰ类 ,巴西陆稻和原产科特迪瓦的陆稻聚在第Ⅰ类的第三小群 ;第Ⅱ类全为籼稻 ,来源于巴基斯坦的籼稻和 1个来源于韩国的籼稻分布在第Ⅱ类 ,说明水稻功能基因在不同亚种、不同产地来源和不同生态类型的水稻之间存在差异 ,也进一步证实水稻功能基因的SSR标记是研究水稻种质资源分类、地理分布、生态类型和系谱分析的有效工具。 | 赵勇 杨凯 Akbar Ali Cheema 翁跃进 | 2002 | 中国农业科学2002，35，4： | 57 |
| 2 | 脑源性神经生长因子促进脑梗死后小鼠内源性神经干细胞的增殖及向神经元分化显示文摘目的:探讨脑源性神经生长因子是否能促进脑梗死后小鼠内源性神经干细胞的活跃,能否刺激其分化成神经元。方法:实验于2004-07/2005-02在墨尔本大学完成。选取10周龄纯种C57BL/6J小鼠24只,分为盐水对照组、脑源性神经生长因子治疗组,每组雌雄各6只。①两组均采用结扎左侧大脑中动脉远心端法建立脑梗死模型,同时采用Matsushita描述的方法监测大脑中动脉供血区的血流量,血流量降低至少75%为有效。②脑源性神经生长因子治疗组于梗死后24h给予脑起源神经营养因子治疗,用药方式采用ALZET锇药物泵缓慢释放。将500μg/kg脑起源神经营养因子溶解于生理盐水中,加入到ALZET泵中,可在28d内持续缓慢释放药物。盐水对照组于梗死后24h给予等量生理盐水。③造模前后对两组小鼠运动功能进行Rotarod测试,记录小鼠在Rotarod上的停留时间。采用双重免疫荧光组化及共焦计数系统检测方法,对两组小鼠内源性神经干细胞的数量、密度及其分化方向进行评估。结果:①运动功能测试结果:与梗死前比较,两组小鼠在梗死后第1周均表现出明显的运动功能下降。但梗死后第2,3,4周,脑起源神经营养因子治疗组小鼠在Rotarod上的停留时间延长,运动功能的恢复明显优于盐水对照组,差异有显著性意义(P<0.01)。②组织切片双重免疫荧光染色结果:两组小鼠脑内均出现免疫荧光阳性表达的内源性神经干细胞,主要分布在病灶周围,在损伤侧的对侧也出现表达。脑起源神经营养因子治疗组的表达数量明显高于盐水对照组。③内源性神经干细胞的激活情况:梗死4周后,两组小鼠脑内都出现内源性神经干细胞的再表达,脑起源神经营养因子治疗组内源性神经干细胞数较盐水对照组明显增加,约4.2倍。同时脑起源神经营养因子治疗组分化为神经元的比例明显高于盐水对照组(36%,15%),分化为星形胶质细胞的比例低于盐水对照组(54%,77%),分化为少突胶质细胞的比例二者基本相似(10%,8%)。结论:脑起源神经营养因子可能是基于内源性神经干细胞治疗脑卒中的一种有效的方法.通过调控内源性神经干细胞的增殖分化来治疗神经系统疾病在未来将是一种新的干细胞治疗方法. | 臧大维 刘娟 左宪华 Surindar Cheema | 2006 | 中国临床康复2006，10，33： | 9 |
| 3 | 温和提取法评估老化前后土壤中菲的生物有效性显示文摘采用温和溶剂提取方法对不同老化时间土壤中的菲进行提取,研究了正丁醇、二氯甲烷、2-羟丙基-β-环糊精(2-Hydroxypropyl-beta cyclodextrin,HPCD)提取量与生物吸收之间的相关性。结果表明,温和有机溶剂(二氯甲烷、正丁醇)提取与温和HPCD提取法对菲的提取量与蚯蚓蓄积量有很好的相关性(p<0.05)。与其他温和试剂提取相比,温和正丁醇提取可以更好地预测菲在土壤中的生物有效性。 | 张聪恺 Muhammad Imran Khan Sardar Alam Cheema 沈超峰 唐先进 申开丽 陈蕾 陈新才 施积炎 陈英旭 | 2011 | 土壤学报2011，48，1： | 5 |
| 4 | 脊髓损伤后神经营养因子及干细胞治疗对轴突再生的影响:国外基础和临床研究新进展(英文)显示文摘背景:近几年国外学者在脊髓损伤的病理机制、损伤后神经元的保护、少突胶质细胞的再生及神经干细胞的移植治疗等研究方面取得了实质性地进展。介绍国外近10年来对脊髓损伤的新认识,最新研究成果及未来的科研和治疗方向。资料来源:应6用计算机检索Medline数据库1987-01/2006-10脊髓损伤的相关文章,限定文章语言种类为English,检索词为“脊髓损伤;神经干细胞;轴突;神经营养因子;动物模型”,进行不同组合,选出相关文章。资料选择:对资料进行初审,选择脊髓研究中的与神经干细胞及神经营养因子有关的研究文献查找全文。纳入标准:①脊髓损伤中以探讨其机制及新治疗方法的文章。②探讨脊髓损伤后轴突再生,生长锥作用,引导再生方向的靶点,突触再形成及功能重建的文章。③神经营养因子和内源性神经干细胞治疗的文章。排除标准:①未被SCI收录的文章,相类似的研究。②无英文摘要的文章。资料提炼:共收集到相关文献1166篇,按上述标准纳入101篇,实际采用61篇,脊髓损伤机制相关文献12篇,轴突再生相关文献14篇,增长锥作用相关文献8篇,少突胶质细胞相关文献8篇,神经干细胞相关文献7篇,神经生长因子相关文献12篇。其余文献均被排除。资料综合:①脊髓损伤功能恢复的基础:损伤的轴突再生及增长;轴突穿透损伤瘢痕区的能力;轴突朝着正确的靶区方向再生;轴突增长到一定程度后停止,终端形成突触,与神经元相接;神经传递功能重建及运动功能重新恢复。②脊髓损伤的神经病理分析:脊髓损伤后的原发性损害、继发性损害。③脊髓损伤的分子生物学机制包括3个方面:对于成年人中枢神经系统损伤后的神经元的发展、再生,神经元通路的建立起着重要的作用轴突增长锥;对轴突的再生起到抑制作用中枢神经系统髓鞘蛋白;细胞膜和细胞内信号传递。④脊髓损伤中起重要作用的细胞和因子:少突胶质细胞,白血病抑制因子和Minocycline,内源性神经干细胞。⑤脊髓损伤动物模型:最常使用的模型是全部离断、部分离断模型和挫伤模型。⑥脊髓损伤研究的前景:已经开始把动物实验中神经营养因子和神经干细胞治疗发现用于临床,如白血病抑制因子在国外已经开始临床Ⅳ期实验,对内源性神经干细胞的诱导调控增殖研究也已经越来越受到重视。结论:神经营养因子干预治疗及神经干细胞治疗使脊髓损伤后的功能恢复成为可能。进一步探讨神经营养因子引起轴突再生的机制,将是脊髓损伤研究领域的未来方向,了解引导调控神经干细胞的增殖和分化方向,将在修复脊髓损伤方面发挥巨大的作用。 | 臧大维 刘娟 Surindar Cheema | 2007 | 中国组织工程研究与临床康复2007，11，14： | 5 |
| 5 | Clostridium paraputrificum septicemia and liver abscess显示文摘We report the first case of a healthy 23-year-old female who underwent an interventional radiology-guided embolization of a hepatic adenoma, which resulted in a gas forming hepatic liver abscess and septicemia by Clostridium paraputrificum. A retrospective review of Clostridial liver abscesses was performed using a Pub Med literature search, and we found 57 clostridial hepatic abscess cases. The two most commonly reported clostridial species are C. perfringens and C. septicum(64.9% and 17.5% respectively). C. perfringens cases carried a mortality of 67.6% with median survival of 11 h, and 70.2% of the C. perfringens cases experienced hemolysis. All C. septicum cases were found to have underlying liver malignancy at the time of the presentation with a mortality of only 30%. The remaining cases were caused by various Clostridium species, and this cohort's clinical course was significantly milder when compared to the above C. perfringens and C. septicum cohorts. | Yong K Kwon Faiqa A Cheema Bejon T Maneckshana Caroline Rochon Patricia A Sheiner | 2018 | World Journal of Hepatology2018，10，3： | 4 |
| 6 | Brain-derived neurotrophic factors increase the proliferation and differentiation of endogenous neural stem cells in mouse models of cerebral infarction显示文摘BACKGROUND: It has been confirmed that brain-derived neurotrophic factor (BDNF) can promote the proliferation of neural stem cells (NSCs) and protect neuron-like cells in vitro. However, its effect on endogenous NSCs in vivo is still unclear. OBJECTIVE: To evaluate whether BDNF can induce the endogenous NSCs to proliferate and differentiate into the neurons in the mice model of cerebral infarction. DESIGN: A synchronal controlled observation. SETTINGS: Department of Neurology, Microbiology Division of the Department of Laboratory, Tianjin First Central Hospital; Howard Florey Institute, Medical College, the University of Melbourne. MATERIALS: Twenty-four pure breed C57BL/6J mice at the age of 10 weeks old (12 males and 12 females) were divided into saline control group and BDNF-treated group, 6 males and 6 females in each group. METHODS: The experiments were performed at the University of Melbourne from July 2004 to February 2005. ① The left middle cerebral artery (MCA) was ligated in both groups to establish models of cerebral infarction and the Matsushita measuring method was used to monitor the blood flow of the lesioned region supplied by MCA. 75% reduction of blood flow should be reached in the lesioned region. ② At 24 hours after infarction, mice in the BDNF-treated group were administrated with BDNF, which was slowly delivered using an ALZET osmium pump design. BDNF was dissolved in saline at the dosage of 500 mg/kg and injected into the pump, which could release the solution consistently in the following 28 days. The mice in the saline control group accepted the same volume of saline at 24 hours after infarction. ③ The Rotarod function test began at 1 week preoperatively, the time stayed on Rotarod was recorded. The mice were tested once a day till the end of the experiment. At 4 weeks post cerebral infarction, double labeling of Nestin and GFAP, BIII tubulin and CNPase immunostaining was performed to observe the differentiation directions of the re-expressed endogenous NSCs, and the percentages of the cells differentiated into astrocytes, neurons and oligodendrocytes were calculated. MAIN OUTCOME MEASURES: ① The differentiation directions of the re-expressed endogenous NSCs, and the percentage of the cells differentiated into astrocytes, neurons and oligodendrocytes. ② Comparison of motor function between the two groups. RESULTS: All the 24 pure C57BL/6J mice were involved in the analysis of results. ① Positively expressed endogenous NSCs appeared in the mice of both groups, and they mainly distributed around the focus of lesion, as well as the contralateral side. The expressed cells in the BDNF-treated group were obviously more than those in the saline control group. ②Activations of endogenous NSCs: At 4 weeks after infarction, re-expressions of endogenous NSCs appeared in both groups. The number of the re-expressed cells in the BDNF-treated group was about 4.2 times higher than that in the saline control group. The percentage of the cells differentiated into neurons in the BDNF-treated group was significantly higher than that in the saline control group (36%, 15%), the percentage of the cells differentiated into astrocytes was lower than that in the saline control group (54%, 77%), whereas the percentage of the cells differentiated into oligodendrocytes was similar to that in the saline control group (10%, 8%). ③ Results of motor functional test: Compared with before cerebral infarction, the mice in both groups manifested as obvious decrease in motor function at 1week after infarction, whereas the recovery of motor function in the BDNF-treated group was significantly superior to that in the saline control group at 2, 3 and 4 weeks (P < 0.01). CONCLUSION: BDNF can promote the proliferation of endogenous NSCs in the brain of mice with cerebral infarction, it can decrease the differentiation rate of astrocytes, and increase the differentiation rate of neurons. BDNF has small influence on the differentiation of endogenous NSCs into oligodendrocytes, which was not benefit for the recovery of neural axon. Endogenous NSCs may improve the motor function of mice through the above pathways. | Dawei Zang Juan Liu Xianhua Zuo Surindar Cheema | 2007 | Neural Regeneration Research2007，2，3： | 4 |
| 7 | Utilization of metabolomics to identify serum biomarkers for hepatocellular carcinoma in patients with liver cirrhosis显示文摘 | Habtom W. Ressom Jun Feng Xiao Leepika Tuli Rency S. Varghese Bin Zhou Tsung-Heng Tsai Mohammad R. Nezami Ranjbar Yi Zhao Jinlian Wang Cristina Di Poto Amrita K. Cheema Mahlet G. Tadesse Radoslav Goldman Kirti Shetty | 2012 | Analytica Chimica Acta2012，，： | 2 |
| 8 | Rice In Vivo RNA Structurome Reveals RNA Secondary Structure Conservation and Divergence in Plants显示文摘RNA 第二等的结构在基因规定起一个关键作用。瑞斯(Oryza sativa ) 是在世界上的最重要的食物庄稼之一。然而,在米饭的 RNA 结构几乎没被学习。这里,我们成功地产生了在 ? 在米饭的 vivo Structure-seq 图书馆。我们发现 mRNAs 的结构的灵活性可能与生物功能的动力学联系。更高的 N 6-methyladenosine (m 6 一) 修正趋于在 3 UTR 有更少的 RNA 结构,而 GC 内容显著地不影响在 ? 维持象翻译那样的有效生物过程的 vivo mRNA 结构。在米饭和 Arabidopsis 之间的 RNA structurome 的比较分析揭示了内容不导致的那更高的 GC 更强壮的结构和更少 RNA 结构的灵活性。而且,我们发现了在顺序和在米饭和 Arabidopsis 之间的 orthologs 的结构保存之间的弱关联。顺序并且在的保存和分叉 ? vivo RNA 结构对应于多样、特定的生物过程。我们的结果显示那 RNA 第二等的结构可能把选择的分开的层提供给在 monocot 和 dicot 之间的顺序。因此,我们的学习暗示那 RNA 结构在各种各样的生物进程不同地演变在被子植物进化期间在开发和 adaptational 灵活性维持坚韧性。 | Hongjing Deng Jitender Cheema Hang Zhang Hugh Woolfenden Matthew Norris Zhenshan Liu Qi Liu Xiaofei Yang Minglei Yang Xian Deng Xiaofeng Cao Yiliang Ding | 2018 | Molecular Plant2018，11，4： | 2 |
| 9 | An overview on biochar production, its implications, and mechanisms of biochar-induced amelioration of soil and plant characteristics显示文摘The degradation of soil fertility and quality due to rapid industrialization and human activities has stimulated interest in the rehabilitation of low-fertility soils to sustainably improve crop yield. In this regard, biochar has emerged as an effective multi-beneficial additive that can be used as a medium for the amelioration of soil properties and plant growth. The current review highlights the methods and conditions for biochar production and the effects of pyrolysis temperature, feedstock type, and retention time on the physicochemical properties of biochar. We also discuss the impact of biochar as a soil amendment with respect to enhancing soil physical (e.g., surface area, porosity, ion exchange, and water-holding capacity) and chemical (e.g., pH, nutrient exchange,functional groups, and carbon sequestration) properties, improving the soil microbiome for increased plant nutrient uptake and growth, reducing greenhouse gas emissions, minimizing infectious diseases in plants, and facilitating the remediation of heavy metal-contaminated soils. The possible mechanisms for biochar-induced amelioration of soil and plant characteristics are also described, and we consider the challenges associated with biochar utilization. The findings discussed in this review support the feasibility of expending the application of biochar to improve degraded soils in industrial and saline-alkali regions, thereby increasing the usable amount of cultivated soil. Future research should include long-term field experiments and studies on biochar production and environmental risk management to optimize biochar performance for specific soil remediation purposes. | Fasih Ullah HAIDER Jeffrey ACOULTER Liqun CAI Saddam HUSSAIN Sardar Alam CHEEMA Jun WU Renzhi ZHANG | 2022 | Pedosphere2022，32，1： | 2 |
| 10 | Efficacy of natural biocide on control of microbial induced corrosion in oil pipelines mediated by Desulfovibrio vulgaris and Desulfovibrio gigas显示文摘We compared the efficacy of a natural biocide with four chemical tetrakishydroxymethyl phosphonium sulfonate,benzyl trimethyl ammonium chloride,and formaldehyde,glutaraldehyde,to control microbial induced corrosion in oil pipelines.The efficacy of biocides were monitored against Desulfovibrio vulgaris and Desulfovibrio gigas in experimental pipes by measuring cell counts,H 2 S production,Fe(Ⅱ) production,production of extracellular polymeric substances and structure of biofilm.The treatment with cow urine hadminimum planktonic cell counts of 3×10 2 CFU/mL as well as biofilm cell counts of 9×10 1 CFU/mL as compared with tetrakishydroxyl methyl phosphonium sulfonate,benzyl trimethyl ammonium chloride,formaldehyde and glutaraldehyde.Sulfide production was the lowest with cow urine (0.08 mmol/L),followed by tetrakishydroxymethyl phosphonium sulfonate 0.72 mmol/L.On day 90 of treatment,Fe(Ⅱ) production was also found to be the lowest with cow urine.The scanning electron microscopic studies indicated that the biofilm bacteria were killed by cow urine.These results demonstrate the cow urine mediated control of microbially induced corrosion,and this is indicative of its potential as a viable substitute of toxic biocides.To the best of our knowledge,this seems to be the first report which screens possible biocidal activity by cow urine as compared to the most common biocides which oil industry is currently using. | Meeta Lavania Priyangshu M.Sarma Ajoy K.Mandal Simrita Cheema Banwari Lal | 2011 | Journal of Environmental Sciences2011，23，8： | 2 |
| 11 | 白血病抑制因子对转基因SOD1小鼠内源性神经干细胞分化的调控显示文摘背景:肌萎缩侧索硬化后运动神经元存在神经变性改变,但其确切机制不明,内源性神经干细胞被认为是未来能有效解决此病的治疗方法之一。目的:探讨白血病抑制因子对肌萎缩侧索硬化转基因小鼠脑干内源性神经干细胞的激活及分化方向的影响。设计、时间及地点:重复观察测量,动物功能学与细胞免疫组化学实验,主要于2006-03/2008-04在天津市第一中心医院完成,部分实验于2004-01/05在澳大利亚墨尔本大学医学院完成。材料:由美国Jackson实验室提供的起源于B6SJL-TgN(SOD1-G93A)、表达突变人类超氧化物歧化酶1基因的转基因小鼠108只,随机均分为正常对照组、肌萎缩侧索硬化组、治疗组,每组动物分别在出生后60,90,120d各取12只用于实验,雌雄各半。方法:各组小鼠均于出生后57d起开始进行运动功能测试,记录小鼠在Rotarod上的停留时间,最大测定时间为180s,每天测试1次,直到实验结束。于出生后90,120d取材的小鼠,从出生后60d开始接受药物干预,治疗组腹腔内注射含25μg/kg白血病抑制因子的生理盐水,正常对照组与肌萎缩侧索硬化组等量注射单纯的生理盐水,1次/d。分别于出生后60,90,120d终止相应实验,分离小鼠脑干,采用双重免疫荧光组化法标记内源性神经干细胞。主要观察指标:运动功能,内源性神经干细胞的激活,激活后细胞分化方向。结果:出生后60d,各组动物均能在Rotarod上停留达到最大测试时间180s,脑干均未发现明显的内源性神经干细胞激活。出生后90,120d,正常对照组仍能达到Rotarod最大测试时间180s,仍没有明显的内源性神经干细胞激活;肌萎缩侧索硬化组、治疗组小鼠在Rotarod上的停留时间均明显降低,但后者降低程度明显小于前者(P<0.01);肌萎缩侧索硬化组、治疗组均出现明显的内源性神经干细胞激活,且后者出现激活的细胞数量明显高于前者(P<0.05;P<0.01)。出生120d时与肌萎缩侧索硬化组比较,治疗组内源性神经干细胞分化为星形胶质细胞的比例明显下降(P<0.01),分化成神经元及少突胶质细胞的比例明显上升(P<0.01;P<0.05)。结论:白血病抑制因子是一种能提升内源性神经干细胞的激活、调控其朝向神经元及少突胶质细胞分化的神经营养因子。 | 臧大维 刘娟 Cheema SS | 2008 | 中国组织工程研究与临床康复2008，12，34： | 2 |
| 12 | Leukemia inhibilory factor rescues motoneurones fxom axotomy-induced cell death显示文摘 | Cheema SS RichardsLJ MurphyM Bartlett PF | 1994 | Neurore-port1994，5，： | 1 |
| 13 | Progressive resistance training in breast cancer:a systematic review of clinical trials显示文摘 | Cheema B Gaul CA Lane K | 2008 | Breast Cancer Res Treat2008，109，1： | 1 |
| 14 | 显示文摘 | Cheema SK Agellon LB | 1999 | J Nutr1999，129，： | 1 |
| 15 | Increasing the efficiency of sorghum water extract by mixing with lower doses of isoproturon to control weeds in wheat 显示文摘 | Jamil M Cheema Z A Khaliq A | 2005 | International Journal of Agriculture & Biology2005，7，5： | 1 |
| 16 | Role of combined cataract surgery and intravitreal bevaeizumab injection in preventing progression of diabetic patients 显示文摘 | Cheema RA A1-Mubarak MM Amin YM | 2009 | J Cataract Refract Surg2009，35，： | 1 |
| 17 | Feasibility of tidal volume-guided ventilation in newborn infants : a randomized, crossover trial using the volume guarantee modality 显示文摘 | Cheema IU Ahluwalia JS | 2001 | Pediatrics2001，107，： | 1 |
| 18 | Triamicinolone acetonide as an aid to visualization of the vitreous and posterior hyaloid during pars plana vitrectomy显示文摘 | Peyman GA Cheema R Conmay MD | 2000 | Retina2000，20，： | 1 |
| 19 | Effects of morphine anal- gesia on diagnostic accuracy in Emergency Department patients with abdominal pain: a prospective, randomized trial 显示文摘 | Thomas SH SilenW Cheema F | 2003 | J Am Coll Surg2003，196，1： | 1 |
| 20 | Degenerative and regenerative mechanisms governing spinal cord injury显示文摘 | Profyris C Cheema SS Zang D | 2004 | Neurobiol Dis2004，15，3： | 1 |
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