|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | A small natural molecule promotes mitochondrial fusion through inhibition of the deubiquitinase USP30显示文摘Mitochondrial 熔化是为正常 mitochondrial 功能和 mitochondrial DNA 继承混合并且统一 mitochondrial 分隔空间的一个高度协调的过程。Dysregulated mitochondrial 熔化原因 mitochondrial 破碎,反常 mitochondrial 生理学和继承,并且有原因地与很多 neuronal 疾病被连接了。这里,我们识别了 potently 导致了 mitochondrial 熔化在在 Mfn1 或 Mfn2 是缺乏的房间恢复 mitochondrial 网络和氧化呼吸的 diterpenoid 衍生物 15-oxospiramilactone (S3 ) 。线粒体局部性的 deubiquitinase USP30 是 S3 的一个目标。由 S3 的 USP30 的抑制导致 Mfn1/2 的 non-degradative ubiquitination 的增加,它提高 Mfn1 和 Mfn2 活动并且支持 mitochondrial 熔化。通过 USP30 的一个禁止者的使用,因此,我们的学习揭开在支持 mitochondrial 的 Mfns 的 non-degradative ubiquitination 的异乎寻常的功能熔化。 | Wen Yue Ziheng Chen HaiYang Li Chen Yan Ming Chen Du Feng Chaojun Yan Hao Wu Lei Du Yueying Wang Jinhua Liu Xiaohu Huang Laixin Xia Lei Liu Xiaohui Wang Haijing Jin Jun Wang Zhiyin Song Xiaojiang Hao Quan Chen | 2014 | Cell Research2014，24，4： | 18 |
| 2 | A New Indicator for Global Food Security Assessment: Harvested Area Rather Than Cropland Area显示文摘Cropland area has long been used as a key indicator of food security.However,grain yield is not solely controlled by the area of the cropland.Therefore,we proposed a new indicator to assess food security.Results show that from 1992 to 2004,the global cropland area increased by 840200 km^(2)(99.4%),but the grain yield increased only by 310 million t(29.1%);and from 2004 to 2015,the cropland area decreased by 39000 km^(2)(4.64%),but the grain yield increased by 370 million t(70.84%).This result showed that grain yield was not linearly correlated with cropland area,and delimiting the threshold of cropland protection may not guarantee food security.Combined with further correlation analysis,we found that the increase in the global grain yield was more closely related to the harvested area(R^(2)=0.94),which indicated that the harvested area is a more scientific and accurate indicator than cropland area in terms of guaranteeing food security.Therefore,if governments want to ensure the food security,they should choose a new and more accurate indicator:harvested area rather than cropland area. | SONG Fengjiao WANG Shijie BAI Xiaoyong WU Luhua WANG Jinfeng LI Chaojun CHEN Huan LUO Xuling XI Huipeng ZHANG Sirui LUO Guofeng YAN Mengqi ZHEN Qingqing | 2022 | Chinese Geographical Science2022，32，2： | 2 |
| 3 | Enzyme-linked immunosorbent assay of changes in serum levels of growth hormone(cGH)in common carps(Cyprinus carpio)显示文摘The aim of the present study was to purify the common native carp growth hormone (ncGH), produce monoclonal antibodies (mAbs) to common native carp growth hormone (ncGH), and further enhance the sensitivity of enzyme-linked immunosorbent assays (ELISA) for ncGH. Additionally, we investigated changes in serum ncGH levels in carps raised in different environmental conditions. The recombinant grass carp (Ctenopharyngodon idella) growth hormone was purified and used as antigen to immunize the rabbit. The natural ncGH was isolated from the pituitaries of common carp. SDS-PAGE and Western blot utilizing the polyclonal anti-rgcGH antibody confirmed the purification of ncGH from pituitaries. Purified ncGH was then used as an immunogen in the B lymphocyte hybridoma technique. A total of 14 hybridoma cell lines (FMU-cGH 1-14) were established that were able to stably secrete mAbs against ncGH. Among them, eight clones (FMU-cGH1-6, 12 and 13) were successfully used for Western blot while nine clones (FMU-cGH 1-7, 9 and 10) were used in fluorescent staining and immunohistochemistry. Epitope mapping by competitive ELISA demonstrated that these mAbs recognized five different epitopes. A sensitive sandwich ELISA for detection of ncGH was developed using FMU-cGH12 as the coating mAb and FMU-cGH6 as the enzyme labeled mAb. This detection system was found to be highly stable and sensitive, with detection levels of 70 pg/mL. Additionally, we found that serum ncGH levels in restricted food group and in the net cage group increased 6.9-and 5.8-fold, respectively, when compared to controls, demonstrating differences in the GH stress response in common carp under different living conditions. | WU Gang CHEN LiHua ZHONG Shan LI Qi SONG ChaoJun JIN BoQuan ZHU ZuoYan | 2008 | Science China(Life Sciences)2008，51，2： | 1 |
| 4 | A carbon-neutrality-capactiy index for evaluating carbon sink contributions显示文摘The accurate determination of the carbon-neutrality capacity(CNC)of a region is crucial for developing policies related to emissions and climate change.However,a systematic diagnostic method for determining the CNC that considers the rock chemical weathering carbon sink(RCS)is lacking.Moreover,it is challenging but indispensable to establish a fast and practical index model to determine the CNC.Here,we selected Guizhou as the study area,used the methods for different types of carbon sinks,and constructed a CNC index(CNCI)model.We found that:(1)the carbonate rock chemical weathering carbon sink flux was 30.3 t CO_(2)km^(-2)yr^(-1).Guizhou accounted for 1.8%of the land area and contributed 5.4%of the carbonate chemical weathering carbon sink;(2)the silicate rock chemical weathering carbon sink and its flux were 1.44×10^(3)t CO_(2)and 2.43 t CO_(2)km^(-2)yr^(-1),respectively;(3)the vegetation-soil ecosystem carbon sink and its flux were 1.37×10^(8)t CO_(2)and 831.70 t CO_(2)km^(-2)yr^(-1),respectively;(4)the carbon emissions(CEs)were 280 Tg CO_(2),about 2.8%of the total for China;and(5)the total carbon sinks in Guizhou were 160 Tg CO_(2),with a CNCI of 57%,which is 4.8 times of China and 2.1 times of the world.In summary,we conducted a systematic diagnosis of the CNC considering the RCS and established a CNCI model.The results of this study have a strong implication and significance for national and global CNC determination and gap analysis. | Xiaoyong Bai Sirui Zhang Chaojun Li Lian Xiong Fengjiao Song Chaochao Du Minghui Li Qing Luo Yingying Xue Shijie Wang | 2023 | Environmental Science and Ecotechnology2023，，3： | 1 |
| 5 | Membrane-targeting amphiphilic AIE photosensitizer for broad-spectrum bacteria imaging and photodynamic killing of bacteria显示文摘An amphiphilic AIE photosensitizer has been successfully developed,which allows for easily inserting into the bacterial membranes.Binding experiments with phospholipid preliminary demonstrates its membrane specificity.As expected,it is proved to possess a broad-spectrum bacterial staining performance and photodynamic antibacterial activity toward S.aureus and E.coli. | Haining Zhang Chaojun He Limin Shen Wenjun Tao Jianhui Zhu Jinzhao Song Ziyong Li Jun Yin | 2023 | Chinese Chemical Letters2023，34，9： | 0 |
| 6 | Storage, form, and influencing factors of karst inorganic carbon in a carbonate area in China显示文摘Carbonate rock chemical weathering carbon sinks reduce the rate of increase of the atmospheric CO_(2) concentration and global warming. However, uncertainty still exists in the estimation results of carbonate rock chemical weathering carbon sink fluxes(CCSF), and the contributions of climate change and ecological restoration to the CCSF are not clear. To this end, we compiled published site data on ion concentrations in different watersheds in China and used a classical thermodynamic dissolution model to reassess the potential and spatial and temporal patterns of the CCSF in China from 1991 to 2020. We quantified the contributions of temperature(MAT), precipitation(MAP), evapotranspiration(ET), soil water(SM), and the normalized difference vegetation index(NDVI) to the CCSF. The results revealed that(1) China's CCSF was 22.76 t CO_(2)km^(-2)yr^(-1), which was higher than the global average(15.77 t CO_(2)km^(-2)yr^(-1)). The total carbonate rock chemical weathering carbon sink(CCS) was 4772.67×10^(4)t CO_(2), contributing 14.91% of the global CCS through a carbonate rock area of 252.98×10^(4)km^(-2).(2) China's CCSF decreased gradually from southeast to northwest, with values of 33.14, 12.93, and7.27 t CO_(2)km^(-2)yr^(-1)in the southern karst, Qinghai-Tibetan karst, and northern karst regions, respectively.(3) The overall CCSF in China exhibited an increasing trend from 1991 to 2020, with a rate of increase of 0.16 t CO_(2)km^(-2)yr^(-1).(4) The contributions of the MAP, MAT, ET, SM, and NDVI to the CCSF were 63.3%, 3.02%, 27.5%, 3.1%, and 3.05%, respectively. Among them, the increase in precipitation was the main contributor to the increase in the CCSF in China over the last 30 years, while the enhancement of ET offset part of the positive contribution of the increase in precipitation to the CCSF. In conclusion, the results of this study provide a systematic quantification of the magnitude, the patterns, and the influencing factors of CCS over a long time series in China. The results are of great significance and provide a reference for the diagnosis and gap analysis of the national and global carbon neutrality capacities. | Chaochao DU Xiaoyong BAI Yangbing LI Qiu TAN Cuiwei ZHAO Guangjie LUO Luhua WU Fei CHEN Chaojun LI Chen RAN Sirui ZHANG Lian XIONG Fengjiao SONG Biqin XIAO Zilin LI Yingying XUE Mingkang LONG Qing LUO Xiaoyun ZHANG Minghui LI Xiaoqian SHEN Shu YANG | 2024 | Science China Earth Sciences2024，67，3： | 0 |
| 7 | Establishment of NaLuF_(4):15%Tb-based low dose X-PDT agent and its application on efficient antitumor therapy显示文摘X-ray excited photodynamic therapy(X-PDT)is the bravo answer of photodynamic therapy(PDT)for deep-seated tumors,as it employs X-ray as the irradiation source to overcome the limitation of light penetration depth.However,high X-ray irradiation dose caused organ lesions and side effects became the major barrier to X-PDT application.To address this issue,this work employed a classic-al co-precipitation reaction to synthesize NaLuF_(4):15%Tb^(3+)(NLF)with an average particle size of(23.48±0.91)nm,which was then coupled with the photosensitizer merocyanine 540(MC540)to form the X-PDT system NLF-MC540 with high production of singlet oxygen.The system could induce antitumor efficacy to about 24%in relative low dose X-ray irradiation range(0.1-0.3 Gy).In vivo,when NLF-MC540 irradiated by 0.1 Gy X-ray,the tumor inhibition percentage reached 89.5%±5.7%.The therapeutic mechanism of low dose X-PDT was found.A significant increase of neutrophils in serum was found on the third day after X-PDT.By immunohistochemical staining of tumor sections,the Ly6G^(+),CD8^(+),and CD11c^(+)cells infiltrated in the tumor microenvironment were studied.Utilizing the bilat-eral tumor model,the NLF-MC540 with 0.1 Gy X-ray irradiation could inhibit both the primary tumor and the distant tumor growth.De-tected by enzyme linked immunosorbent assay(ELISA),two cytokines IFN-γand TNF-αin serum were upregulated 7 and 6 times than negative control,respectively.Detected by enzyme linked immune spot assay(ELISPOT),the number of immune cells attributable to the IFN-γand TNF-αlevels in the group of low dose X-PDT were 14 and 6 times greater than that in the negative control group,respectively.Thus,it conclude that low dose X-PDT system could successfully upregulate the levels of immune cells,stimulate the secretion of cy-tokines(especially IFN-γand TNF-α),activate antitumor immunity,and finally inhibit colon tumor growth. | Yi Tian Zhiguang Fu Xiaosheng Zhu Chunjing Zhan Jinwei Hu Li Fan Chaojun Song Qian Yang Yu Wang Mei Shi | 2024 | International Journal of Minerals,Metallurgy and Materials2024，31，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员