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| 1 | Fiber-tip polymer clamped-beam probe for high-sensitivity nanoforce measurements显示文摘Micromanipulation and biological,material science,and medical applications often require to control or measure the forces asserted on small objects.Here,we demonstrate for the first time the microprinting of a novel fiber-tip-polymer clamped-beam probe micro-force sensor for the examination of biological samples.The proposed sensor consists of two bases,a clamped beam,and a force-sensing probe,which were developed using a femtosecond-laser-induced two-photon polymerization(TPP)technique.Based on the finite element method(FEM),the static performance of the structure was simulated to provide the basis for the structural design.A miniature all-fiber micro-force sensor of this type exhibited an ultrahigh force sensitivity of 1.51 nmμN−1,a detection limit of 54.9 nN,and an unambiguous sensor measurement range of~2.9 mN.The Young’s modulus of polydimethylsiloxane,a butterfly feeler,and human hair were successfully measured with the proposed sensor.To the best of our knowledge,this fiber sensor has the smallest force-detection limit in direct contact mode reported to date,comparable to that of an atomic force microscope(AFM).This approach opens new avenues towards the realization of small-footprint AFMs that could be easily adapted for use in outside specialized laboratories.As such,we believe that this device will be beneficial for high-precision biomedical and material science examination,and the proposed fabrication method provides a new route for the next generation of research on complex fiber-integrated polymer devices. | Mengqiang Zou Changrui Liao Shen Liu Cong Xiong Cong Zhao Jinlai Zhao Zongsong Gan Yanping Chen Kaiming Yang Dan Liu Ying Wang Yiping Wang | 2021 | Light(Science & Applications)2021,10,9: | 3 |
| 2 | 3D printed fiber-optic nanomechanical bioprobe显示文摘Ultrasensitive nanomechanical instruments,e.g.atomic force microscopy(AFM),can be used to perform delicate biomechanical measurements and reveal the complex mechanical environment of biological processes.However,these instruments are limited because of their size and complex feedback system.In this study,we demonstrate a miniature fiber optical nanomechanical probe(FONP)that can be used to detect the mechanical properties of single cells and in vivo tissue measurements.A FONP that can operate in air and in liquids was developed by programming a microcantilever probe on the end face of a single-mode fiber using femtosecond laser two-photon polymerization nanolithography.To realize stiffness matching of the FONP and sample,a strategy of customizing the microcantilever’s spring constant according to the sample was proposed based on structure-correlated mechanics.As a proof-of concept,three FONPs with spring constants varying from 0.421 N m^(−1)to 52.6 N m^(−1)by more than two orders of magnitude were prepared.The highest microforce sensitivity was 54.5 nmμN^(−1)and the detection limit was 2.1 nN.The Young’s modulus of heterogeneous soft materials,such as polydimethylsiloxane,muscle tissue of living mice,onion cells,and MCF-7 cells,were successfully measured,which validating the broad applicability of this method.Our strategy provides a universal protocol for directly programming fiber-optic AFMs.Moreover,this method has no special requirements for the size and shape of living biological samples,which is infeasible when using commercial AFMs.FONP has made substantial progress in realizing basic biological discoveries,which may create new biomedical applications that cannot be realized by current AFMs. | Mengqiang Zou Changrui Liao Yanping Chen Lei Xu Shuo Tang Gaixia Xu Ke Ma Jiangtao Zhou Zhihao Cai Bozhe Li Cong Zhao Zhourui Xu Yuanyuan Shen Shen Liu Ying Wang Zongsong Gan Hao Wang Xuming Zhang Sandor Kasas Yiping Wang | 2023 | International Journal of Extreme Manufacturing2023,5,1: | 1 |
| 3 | Effects of SiC interphase by chemical vapor deposition on the properties of C/ZrC composite prepared via precursor infiltration and pyrolysis route显示文摘 | Si’an Chen Yudi Zhang Changrui Zhang Dan Zhao Haifeng Hu Zhibin Zhang | 2013 | Materials and Design2013,,: | 1 |
| 4 | Recent progress in transition-metal-oxide-based electrocatalysts for the oxygen evolution reaction in natural seawater splitting: A critical review显示文摘Direct electrolytic splitting of seawater for the production of H2 using ocean energy is a promising technology that can help achieve carbon neutrality.However,owing to the high concentrations of chlorine ions in seawater,the chlorine evolution reaction always competes with the oxygen evolution reaction(OER)at the anode,and chloride corrosion occurs on both the anode and cathode.Thus,effective electrocatalysts with high selectivity toward the OER and excellent resistance to chloride corrosion should be developed.In this critical review,we focus on the prospects of state-of-the-art metal-oxide electrocatalysts,including noble metal oxides,non-noble metal oxides and their compounds,and spinel-and perovskite-type oxides,for seawater splitting.We elucidate their chemical properties,excellent OER selectivity,outstanding anti-chlorine-corrosion performance,and reaction mechanisms.In particular,we review metal oxides that operate at high current densities,near industrial application levels,based on special catalyst design strategies. | Meng Chen Nutthaphak Kitiphatpiboon Changrui Feng Abuliti Abudula Yufei Ma Guoqing Guan | 2023 | eScience2023,3,2: | 1 |
| 5 | Effects of TaC amount on the properties of 2D C/SiC–TaC composites prepared via precursor infiltration and pyrolysis显示文摘 | Si’an Chen Haifeng Hu Yudi Zhang Changrui Zhang Qikun Wang | 2013 | Materials and Design2013,,: | 1 |
| 6 | A Probe-Shaped Sensor With FBG and Fiber-Tip Bubble for Pressure and Temperature Sensing显示文摘A probe-shaped sensor for simultaneous temperature and pressure measurement was reported in this article.The effective length of the sensor was〜2 mm,consisting of a fiber Bragg grating(FBG)and a Fabry-Perot interferometer(FPI)with a nano silica diaphragm.The response sensitivities of the sensor for pressure and temperature were measured as-0.98 nm/MPa and 11.10pm/℃,respectively.This sensor had an extremely low cross-sensitivity between pressure and temperature,which provided a significant potential in dual-parameter sensing. | Bonan LIU Junxian LUO Shen LIU Yanping CHEN Bo HUANG Changrui LIAO Yiping WANG | 2021 | Photonic Sensors2021,11,4: | 1 |
| 7 | Turning gas hydrate nucleation with oxygen-containing groups on size-selected graphene oxide flakes显示文摘Gas hydrate is a promising alternative for gas capture and storage due to its high gas storage capacity achieved with only structured water molecules.Nucleation is the critical controlling step in gas hydrate formation.Adding an alien solid surface is an effective approach to regulate gas hydrate nucleation.However,how the solid surface compositions control the gas hydrate nucleation remains unclear.Benefiting from the fact that the surface compositions of graphene oxide(GO)can be finely tuned,we report the effect of functional groups of size-selected GO flakes on methane hydrate nucleation.The carbonyl and carboxyl of GO flakes showed a more prominent promotion for methane hydrate nucleation than the hydroxyl of GO flakes.Surface energy,zeta potential,Raman spectra,and molecular dynamics simulation analysis were used to reveal the regulation mechanism of the functional groups of size-selected GO flakes on methane hydrate nucleation.The GO flakes with abundant carbonyl and carboxyl exhibited higher charge density than those enriched in hydroxyl.The negatively charged GO flakes can induce water molecules to form an ordered hydrogen-bonded arrangement via charge-dipole interactions.Therefore,the water molecules surrounding the carboxyl and carbonyl showed a more ordered hydrogen-bonded structure than those around the hydroxyl of GO flakes.The ordered water arrangement,similar to methane hydrate cages,significantly accelerated methane hydrate nucleation.Our study shows how the surface chemistry of solids control gas hydrate nucleation and sheds light on the design of effective heterogeneous nucleators for gas hydrate. | Huiquan Liu Changrui Shi Zherui Chen Shuai Wang Mingjun Yang Jiafei Zhao Cong Chen Yongchen Song Zheng Ling | 2023 | Journal of Energy Chemistry2023,,12: | 0 |
| 8 | Electrocatalytic seawater splitting for hydrogen production:Recent progress and future prospects显示文摘Earth-abundant seawater resource has become an attractive candidate to produce hydrogen from electrolysis,which is of great significance to realize hydrogen economy and carbon neutrality.Nonetheless,developing highly active and stable electrocatalysts to meet the needs of highly effective seawater splitting is still challenging for the sluggish oxygen evolution dynamics and the existed competitive reaction of chlorine evolution reaction(CER).To this end,some newly-developed electrocatalysts with superior performance,such as noble metals,alloy,transition metals,oxides,carbides,nitrides,phosphides,and so on,have been synthesized for the seawater splitting in recent years.This review starts from the historical background and fundamental mechanisms,and summarizes the most recent progress in the development of seawater electrolysis technologies.Some existing issues in the process of seawater electrolysis are enumerated and the corresponded solutions are presented.The future of hydrogen production from seawater electrolysis,especially the design and synthesis of novel catalysts for seawater electrolysis,is prospected. | Changrui Feng Meng Chen Ziyuan Yang Zhengkun Xie Xiumin Li Shasha Li Abuliti Abudula Guoqing Guan | 2023 | Journal of Materials Science & Technology2023,,31: | 0 |
| 9 | Optimization of RNA Secondary Structure Prediction with SHAPE(Selective 2'-hydroxyl Acylation Analyzed by Primer Extension)Chemistry显示文摘SHAPE(selective 2'-hydroxyl acylation analyzed by primer extension) chemistry has been widely used in the prediction oi RJNA secondary structure at single-nucleotide solution.This study was designed to determine the optimal concentrations of RNA and modifying reagent in SHAPE analysis to improve the accuracy of structure prediction.The results showed that at the ratio of N-methylisatoic anhydride(NMIA)(mmol/L) to RNA(μmol/L) of 13:20,the resulting concentration of full-length RNA was the highest,and the sequencing signal was the strongest.So,this ratio is optimal for the analysis and prediction of RNA secondary structure in SHAPE chemistry. | Jun ZHENG Rujie CAI Altaf SIMAIR Ting CHEN Yunlong ZHANG Changrui LU | 2017 | Agricultural Biotechnology2017,6,1: | 0 |
| 10 | Prediction of the Structure of Full-length Human Telomer-ase RNA with SHAPE(Selective 2'-hydroxyl Acylation An-alyzed by Primer Extension)显示文摘Telomerase is a unique ribonucleoprotein complex that protects chromosomes from degradation and recombination.The defects in telomerase activity caused by the mutations in telomerase RNA(hTER) is associated with several human genetic diseases,so the molecular biology and structural biology of hTER are of great significance for the treatment of related diseases and the development of corresponding drugs.In this study,hTER was obtained by in vitro transcription,and then modified with N-methylisatoic anhydride(NMIA) by SHAPE(selective 2'-hydroxyl acylation analyzed by primer extension);finally,the cDNA fragment was obtained by reverse transcription to analyze the free nucleotides and dynamic structure of hTER,which may provide experimental basis for analyzing three-dimensional structure of hTER obtained in vitro.In addition,the SHAPE parameters for long RNA fragment(> 300 nt) were optimized.The results showed that 1μmol/L hTER and 3.9 mmol/L NMIA were optimal,and 49 ℃ was the best temperature for primer extension. | Xiaoqi QI Yunlong ZHANG Ting CHEN Altaf Ahmed SIMAIR Minmin ZHANG Zhuoru YANG Changrui LU | 2017 | Agricultural Biotechnology2017,6,1: | 0 |
| 11 | Micro powder injection molding of boron carbide components with SiC-Al_(2)O_(3)-Y_(2)O_(3)sintering additives显示文摘Sintering additives and micro-powder injection molding offer an effective method to densify boron carbide(B_(4)C)and make B_(4)C components with complex shapes.By adjusting the proportion of three kinds of powders(SiC,Al_(2)O_(3)and Y_(2)O_(3)),four kinds of sintering additives were prepared.The feedstock uniformity,debinding behavior,phase composition and microstructure of micro injection molded B_(4)C components with different sintering additives were studied.The results showed that the defects such as lattice distortion and vacancy were introduced into ball milling,which increase the surface energy and benefit subsequent sintering densification.The feedstock had good uniformity and rheology that met the requirements of micro powder injection molding.After debinding,B_(4)C components had enough strength and showed certain sintering characteristics.The addition of sintering additives was beneficial to densification and sintering temperature reduction.The addition of sintering additives formed a second phase in the B_(4)C crystal and at the grain boundary of B_(4)C crystal,which changed the fracture mode from transgranular fracture to mixed fracture mechanism with transgranular fracture. | Changrui WANG Zhiyou LI Wei TIAN Yuxian LI Lina TANG Qilong PANG Minghe CHEN | 2022 | Chinese Journal of Aeronautics2022,35,5: | 0 |
| 12 | Micropore engineering on hollow nanospheres for ultra-stable sodium-selenium batteries显示文摘Attracted by high energy density and considerable conductivity of selenium(Se),Na-Se batteries have been deemed promising energy-storage systems.But,it still suffers from sluggish kinetic behaviors and similar“shuttling effect”to S-electrodes.Herein,utilizing uniform hollow carbon spheres as precursors,Se-material is effectively loaded through vapor-infiltration method.Owing to the distribution of optimized pores,the content of microspores could be up to~60%(<2 nm),serving important roles for the physical confinement effect.Meanwhile,the rich oxygen-containing groups and N-elements could be noted,inducing the evolution of electron-moving behaviors.More significantly,assisted by the interfacial C-Se bonds and tiny Se distributions,Se electrodes are activated during cycling.Used as cathodes for Na-Se systems,the as-resulted samples display a capacity of 593.9 mA h g^(-1)after 100 cycles at the current density of 0.1 C.Even after 6000 cycles,the capacity could be still kept at about 225 mA h g^(-1)at 5.0 C.Supported by the detailed kinetic analysis,the designed microspores size induces the increasing redox reaction of nano Se,whilst the surface traits further render the enhancement of pseudo-capacitive contributions.Moreover,after cycling,the product Sex(x<4)in pores serves as the primary active material.Given this,the work is anticipated to provide an effective strategy for advanced electrodes for Na-Se systems. | Gongke Wang Yumeng Chen Yu Han Lixue Yang Wenqing Zhao Changrui Chen Zihao Zeng Shuya Lei Shaohui Yuan Peng Ge | 2023 | Journal of Energy Chemistry2023,,5: | 0 |
| 13 | Expression and Purification of Zinc Finger Domain and Central Domain of MDMD2显示文摘[Objective] This study aimed to construct the His-tagged prokaryotic expression vectors harboring zinc finger domain(ZF) and central domain(acidic domain & zinc finger domain,CT) of MDM2,respectively,and preliminarily identified biologic activity of the purified fusion proteins.[Method]ZF and CT coding regions were amplified from MDM2 c DNA library by PCR and separately inserted into prokaryotic expression vector p ET28 b to construct the recombinant plasmids.After verification by enzyme digestion,the recombinant plasmids were separately transformed into E.coli DH5α competent cells.The expressed recombinant plasmids were purified using Ni-NTA magnetic beads and identified by SDS-PAGE and Western blotting.[Result]The molecular weight of His-ZF and His-CT fusion proteins was 21 and 31 k D,respectively.[Conclusion] Recombinant fusion proteins containing ZF and CT of MDM2 were obtained successfully,which laid the foundation for subsequent protein crystallization and three-dimensional structure analysis. | Xiaoling REN Ting CHEN Yunlong ZHANG Changrui LU Minmin ZHANG Jun ZHENG Xiaoqi QI | 2016 | Agricultural Biotechnology2016,5,2: | 0 |