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您的检索式：作者名="Altaf SIMAIR"
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| 1 | 人端粒酶RNA CR4/5结构域的X射线小角散射分析显示文摘端粒酶是一类独特的核糖核蛋白复合物,能够维持真核生物染色体末端端粒的完整性。目前,人端粒酶全酶分子组装机制及其结构与功能的关系知之甚少,人端粒酶RNA(human telomerase RNA,hTR)的结构研究也仅限于二级结构。通过对hTR CR4/5结构域进行体外转录、纯化,利用X射线小角散射技术(small-angle x-ray scattering,SAXS),获得其在溶液中的聚集信息,并预测分析了hTR CR4/5的构象。这将对hTR CR4/5结构域及hTR三维结构的解析提供有利的数据支持,为了解hTR在端粒酶逆转录过程中的功能提供实验数据。 | 杨卓茹 张敏敏 蔡汝洁 张含笑 管俊豪 Altaf Ahmed Simair 陆昌瑞 | 2018 | 生物学杂志2018，35，4： | 0 |
| 2 | 人源CBX7功能结构域的重组表达与纯化显示文摘CBX7(chromobox 7)在细胞寿命延长和癌症发生中起着重要的调控作用。然而,获取足量的全长CBX7蛋白并用于结构研究极为艰难。实验阐述了CBX7功能结构域CBX7(aa 145~227)重组表达载体构建的详细过程。同时,通过Ni-NTA亲和层析对融合目的蛋白进行了纯化,并利用SDS-PAGE及Western-blot进行了鉴定分析。纯化后的融合蛋白样品通过离子交换和分子筛层析进一步分离剩余杂质。本实验得到的高浓度CBX7蛋白可用于结晶,为后续CBX7的结构和功能研究奠定了基础。 | 胡温温 ALTAF Simair 吕缜一 陈婷 张云龙 陆昌瑞 | 2019 | 生命科学研究2019，23，2： | 0 |
| 3 | Optimization of RNA Secondary Structure Prediction with SHAPE(Selective 2'-hydroxyl Acylation Analyzed by Primer Extension)Chemistry显示文摘SHAPE(selective 2'-hydroxyl acylation analyzed by primer extension) chemistry has been widely used in the prediction oi RJNA secondary structure at single-nucleotide solution.This study was designed to determine the optimal concentrations of RNA and modifying reagent in SHAPE analysis to improve the accuracy of structure prediction.The results showed that at the ratio of N-methylisatoic anhydride(NMIA)(mmol/L) to RNA(μmol/L) of 13:20,the resulting concentration of full-length RNA was the highest,and the sequencing signal was the strongest.So,this ratio is optimal for the analysis and prediction of RNA secondary structure in SHAPE chemistry. | Jun ZHENG Rujie CAI Altaf SIMAIR Ting CHEN Yunlong ZHANG Changrui LU | 2017 | Agricultural Biotechnology2017，6，1： | 0 |
| 4 | Prediction of the Structure of Full-length Human Telomer-ase RNA with SHAPE(Selective 2'-hydroxyl Acylation An-alyzed by Primer Extension)显示文摘Telomerase is a unique ribonucleoprotein complex that protects chromosomes from degradation and recombination.The defects in telomerase activity caused by the mutations in telomerase RNA(hTER) is associated with several human genetic diseases,so the molecular biology and structural biology of hTER are of great significance for the treatment of related diseases and the development of corresponding drugs.In this study,hTER was obtained by in vitro transcription,and then modified with N-methylisatoic anhydride(NMIA) by SHAPE(selective 2'-hydroxyl acylation analyzed by primer extension);finally,the cDNA fragment was obtained by reverse transcription to analyze the free nucleotides and dynamic structure of hTER,which may provide experimental basis for analyzing three-dimensional structure of hTER obtained in vitro.In addition,the SHAPE parameters for long RNA fragment(> 300 nt) were optimized.The results showed that 1μmol/L hTER and 3.9 mmol/L NMIA were optimal,and 49 ℃ was the best temperature for primer extension. | Xiaoqi QI Yunlong ZHANG Ting CHEN Altaf Ahmed SIMAIR Minmin ZHANG Zhuoru YANG Changrui LU | 2017 | Agricultural Biotechnology2017，6，1： | 0 |
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