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您的检索式：作者名="Altaf Ahmed SIMAIR"
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| 1 | 人端粒酶RNA CR4/5结构域的X射线小角散射分析显示文摘端粒酶是一类独特的核糖核蛋白复合物,能够维持真核生物染色体末端端粒的完整性。目前,人端粒酶全酶分子组装机制及其结构与功能的关系知之甚少,人端粒酶RNA(human telomerase RNA,hTR)的结构研究也仅限于二级结构。通过对hTR CR4/5结构域进行体外转录、纯化,利用X射线小角散射技术(small-angle x-ray scattering,SAXS),获得其在溶液中的聚集信息,并预测分析了hTR CR4/5的构象。这将对hTR CR4/5结构域及hTR三维结构的解析提供有利的数据支持,为了解hTR在端粒酶逆转录过程中的功能提供实验数据。 | 杨卓茹 张敏敏 蔡汝洁 张含笑 管俊豪 Altaf Ahmed Simair 陆昌瑞 | 2018 | 生物学杂志2018，35，4： | 0 |
| 2 | Prediction of the Structure of Full-length Human Telomer-ase RNA with SHAPE(Selective 2'-hydroxyl Acylation An-alyzed by Primer Extension)显示文摘Telomerase is a unique ribonucleoprotein complex that protects chromosomes from degradation and recombination.The defects in telomerase activity caused by the mutations in telomerase RNA(hTER) is associated with several human genetic diseases,so the molecular biology and structural biology of hTER are of great significance for the treatment of related diseases and the development of corresponding drugs.In this study,hTER was obtained by in vitro transcription,and then modified with N-methylisatoic anhydride(NMIA) by SHAPE(selective 2'-hydroxyl acylation analyzed by primer extension);finally,the cDNA fragment was obtained by reverse transcription to analyze the free nucleotides and dynamic structure of hTER,which may provide experimental basis for analyzing three-dimensional structure of hTER obtained in vitro.In addition,the SHAPE parameters for long RNA fragment(> 300 nt) were optimized.The results showed that 1μmol/L hTER and 3.9 mmol/L NMIA were optimal,and 49 ℃ was the best temperature for primer extension. | Xiaoqi QI Yunlong ZHANG Ting CHEN Altaf Ahmed SIMAIR Minmin ZHANG Zhuoru YANG Changrui LU | 2017 | Agricultural Biotechnology2017，6，1： | 0 |
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