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| 1 | Integrative single-cell analysis of transcriptome,DNA methylome and chromatin accessibility in mouse oocytes显示文摘Oocyte growth is a key step in forming mature eggs that are ready to be fertilized.The states and modifications of chromatin represent critical sources of information for this process.However,the dynamics and interrelations of these chromatin characteristics remain elusive.In this study,we developed an improved scCOOL-seq technique (iscCOOL-seq),which is a multiomics,single-cell and single-base resolution method with high mapping rates,and explored the chromatin accessibility landscape and its relationship to DNA methylation in growing mouse oocytes.The most dramatic change in chromatin accessibility occurs during oocyte growth initiation,accompanied with prominent transcriptome alterations and an elevated variation in DNA methylation levels among individual oocytes.Unlike CpG islands (CGIs),partially methylated domains (PMDs)are associated with a low density of nucleosome-depleted regions (NDRs)during the whole maturation period.Surprisingly,highly expressed genes are usually associated with NDRs at their transcriptional end sites (TESs).In addition,genes with de novo methylated gene bodies during oocyte maturation are already open at their promoters before oocyte growth initiation.Furthermore,epigenetic and transcription factors that might be involved in oocyte maturation are identified.Our work paves the way for dissecting the complex, yet highly coordinated,epigenetic alterations during mouse oocyte growth and the establishment of totipotency. | Chan Gu Shanling Liu Qihong Wu Lin Zhang Fan Guo | 2019 | Cell Research2019，29，2： | 14 |
| 2 | A Model of Hydrothermal Dolomite Reservoir Facies in Precambrian Dolomite, Central Sichuan Basin, SW China and its Geochemical Characteristics显示文摘Hydrothermal mineral assemblages and related hydrothermally enhanced fracturing are common in the Precambrian Dengying Formation of Central Sichuan Basin. Petrographic and geochemical analyses of core samples show that the hydrothermal dolomite reservoirs of Dengying Formation consist of four main types of pores in the reservoir facies. These include: 1) hydrothermal dissolution vug(or pore), 2) intercrystalline pore, 3) residual inter-breccia vug(or pore), and 4) enlarged dissolved-fracture. There are three different fabrics dolomite in hydrothermal dolomite reservoirs, namely, saddle dolomite, fine-medium dolomite and micritic dolomite. Micritic dolomite is the original lithology of host rock. Saddle dolomite with curved or irregular crystal faces was directly crystallized from hydrothermal fluids(average temperature 192°C). Fine-medium dolomites are the products of recrystallization of micritic dolomite, resulting in abnormal geochemical characteristics, such as slight depletion of δ^(18)O, significant enrichment of Mn-Fe and ^(87)Sr/^(86)Sr, and positive Eu anomaly. A model for the distribution of various hydrothermal dolomite reservoir facies is proposed here, which incorporates three fundamental geological controls: 1) extensional tectonics and tectono-hydrothermal events(i.e., the Xingkai Taphrogenesis of Late Sinian-Early Cambrian, and Emei Taphrogenesis of Late Permian), 2) hydrothermal fluid storage in clastic rocks with large thickness(e.g., Nanhua System of Chengjiang Formation and part of Doushantuo Formation), and 3) confining bed for hydrothermal fluids(such as, the shale in Qiongzhusi Formation). The supply of hydrothermal fluid is critical. Large basement-rooted faults and associated grid-like fracture system may function as the channels for upward migration of hydrothermal fluid flow. The intersection of the above-mentioned faults(including the conversion fault), especially transtensional sags above negative flower structures on wrench faults can serve as a key target for future hydrocarbon exploration. | GU Yifan ZHOU Lu JIANG Yuqiang JIANG Chan LUO Mingsheng ZHU Xun | 2019 | Acta Geologica Sinica(English Edition)2019，93，1： | 5 |
| 3 | Gas sensing capabilities of TiO_(2) porous nanoceramics prepared through premature sintering显示文摘Pure and noble metal(Pt,Pd,and Au)doped TiO_(2)nanoceramics have been prepared from TiO_(2)nanoparticles through traditional pressing and sintering.For those samples sintered at 550℃,a typical premature sintering occurred,which led to the formation of a highly porous microstructure with a Brunauer-Emmett-Teller(BET)specific surface area of 23 m^(2)/g.At room temperature,only Pt-doped samples showed obvious response to hydrogen,with sensitivities as high as~500 for 1000 ppm H_(2)in N_(2);at 300℃,all samples showed obvious responses to CO,while the responses of noble metal doped samples were much higher than that of the undoped ones.The mechanism for the observed sensing capabilities has been discussed,in which the catalytic effect of Pt for hydrogen is believed responsible for the room-temperature hydrogen sensing capabilities,and the absence of glass frit as commonly used in commercial thick-film metal oxide gas sensors is related to the high sensitivities.It is proposed that much attention should be paid to metal oxide porous nanoceramics in developing gas sensors with high sensitivities and low working temperatures. | Yao XIONG Zilong TANG Yu WANG Yongming HU Haoshuang GU Yuanzhi LI Helen Lai Wah CHAN Wanping CHEN | 2015 | Journal of Advanced Ceramics2015，4，2： | 3 |
| 4 | Modified Zhibai Dihuang pill,a traditional Chinese medicine formula,on steroid withdrawal in systemic lupus erythematosus:A systematic review and meta-analysis显示文摘Background:Zhibai Dihuang pill(ZBDH),a Chinese herbal formula,has been widely used as an adjunctive therapy to help reduce the patient’s steroid dose and maintain low disease activity in systemic lupus erythematosus(SLE).Objective:This systematic review evaluates the therapeutic effect of modified ZBDH in reducing steroid use in patients with SLE.Search strategy:A systematic literature search was carried out using seven databases,including PubMed,Embase,Cochrane Central Register of Controlled Trials,Chinese Biomedical Literature Database,Chinese National Knowledge Infrastructure,Chinese VIP Information and Wanfang Database,from their inception to June 1 st,2019.The search terms included‘‘systemic lupus erythematosus,'‘‘Chinese medicine'and‘‘clinical trial,'and their synonyms.Subject headings matching the above terms were also used.Inclusion criteria:This meta-analysis included randomized controlled trials that evaluated the reduction of steroid dose in patients with SLE.Traditional Chinese medicine(TCM)formulas in experimental group should be prescribed based on ZBDH and used as adjunctive therapy and the comparator should contain steroids.Data extraction and analysis:Two authors independently conducted database search,study selection,data extraction and quality assessment.The extracted information contained study design,sample size,recruitment mode,diagnostic criteria,inclusion and exclusion criteria,participant characteristics,TCM patterns,TCM formulas and treatment outcomes.The primary outcome was the change of steroid dose.Secondary outcomes included SLE Disease Activity Index(SLEDAI),biomarkers of disease activity and clinical response rate.STATA 15.0 was used to analyze the pooled effects reported as weighted mean difference(WMD)or odds ratio,with a 95%confidence interval(CI).Results:In total,20 trials involving 1470 SLE patients were included.The pooled result showed that modified ZBDH taken in combination with standard care led to a larger reduction in steroid dose,compared to standard care alone(WMD:3.79;95%CI:2.58–5.01;P<0.001).Favorable outcomes were also seen in secondary outcome criteria,such as SLEDAI and complement 3.The modified ZBDH treatments were well tolerated without increasing adverse effects.Conclusion:The systematic review provided preliminary evidence supporting the use of ZBDH as a cotherapy to aid steroid dose reduction in patients with SLE.However,more rigorous studies should be conducted to validate these findings,and explore the mechanisms of ZBDH’s relevant bioactive constituents. | Liang Dai Ka Kei Chan Jian-chun Mao Yu Tianc Jun-hua Gu Jun Zhou Linda L.D.Zhong | 2020 | Journal of Integrative Medicine2020，18，6： | 3 |
| 5 | S6B-2 Matrine Inhibits Itching by Lowering the Activity of Calcium Channel显示文摘Sophorae Flavescentis Radix(SFR)is a medicinal herb with many functions that are involved in anti-inflammation,antinociception,and anticancer.SFR is also used to treat a variety of itching diseases.Matrine(MT)is one of the main constituents in SFR and also has the effect of relieving itching,but the antipruritic mechanism is still unclear.Here,we investigated the effect of MT on antipruritus.In acute and chronic itch models,MT significantly inhibited the scratching behavior not only in acute itching induced by histamine(His),chloroquine(CQ)and compound 48/80 with a dose-depended manner,but also in the chronic pruritus models of Atopic dermatitis(AD)and Acetone-ether-water(AEW)in mice.Furthermore,MT can be detected in the blood after intraperitoneal injection(i.p.)and subcutaneous injection(s.c.).Finally,electrophysiological and calcium image results show that MT inhibits the excitatory synaptic transmission from dorsal root ganglion(DRG)to the dorsal horn of the spinal cord by suppressing presynaptic N-type calcium channels.Taken together,we believe that MT is a novel drug candidate in treating pruritus diseases,especially for histamine-independent and chronic pruritus,which might be attributed to inhibition of presynaptic N-type calcium channels. | GENG Xiao SHI Hao YE Fan DU Han QIAN Lin-nan GU Le-ying WU Guan-yi ZHU Chan YANG Yan WANG Chang-ming ZHOU Yuan YU Guang LIU Qin DONG Xin-zhong YU Lei 唐宗湘 | 2018 | 神经药理学报2018，8，4： | 2 |
| 6 | Structure of Apolipoprotein B-100 of Human Low Density Lipoproteins显示文摘 | Chao-yuh Yang Zi-Wei Gu Shi-ai Weng Tae W. Kim San-Hwan Chen Henry J. Pownall Paul M. Sharp Shyan-Woei Liu Wen-Hsiung Li Antonio M. Gotto Lawrence Chan | 1989 | Arteriosclerosis, Thrombosis, and Vascular Biology1989，，1： | 2 |
| 7 | Cloning of a cDNA encoding a pu- tative molt-inhibiting hormone from the eyestalk of the sand shrimp Metapenaeus ensis 显示文摘 | Gu P L Chan S M | 1998 | Mol Marine Bio- tech1998，7，3： | 1 |
| 8 | Quantitative assessment of diffusion-weighted MR imaging in patients with primary rectal cancer: correlation with FDG-PET/CT显示文摘 | Gu J Khong PL Wang S Chan Q Law W Zhang J | 2011 | Mol Imaging Biol2011，13，： | 1 |
| 9 | Justice-based Service Recovery Expectations:Measurement and Antecedents显示文摘 | Yim C K Gu F F Chan K W Tse D K | | 0，，16： | 1 |
| 10 | Exact tangent stiffness for imperfect beam-column members显示文摘 | Chan S L Gu J X | 2000 | Journal of Structural Engineering(ASCE)2000，126，： | 1 |
| 11 | Iron overload accelerates devel-opment of leukaemia:evidence from mouse model显示文摘 | Chan LSA Gu LC Rauh MJ | 2010 | Blood2010，116，21： | 1 |
| 12 | Bacterial expression of the shrimp molt-inhibiting hormone (MIH): anti- body production, immunocytochemical study and bio- logical assay显示文摘 | Gu P L Chu K H Chan S M | 2001 | Cell and tissue research2001，303，1： | 1 |
| 13 | Comparison of bioleaehing of heavy metals from sewage sludge using iron-and sulfur-oxidizing bacteria显示文摘 | Chan L C Gu X Y Wong J W C | 2003 | Adv Environ Res2003，7，： | 1 |
| 14 | Product modeling using STEP 显示文摘 | GU P CHAN K | 1995 | Computer-Aided Design1995，27，3： | 1 |
| 15 | Oral silibinin inhibits lung tumor growth in athymic nude mice and forms a novel chemocombination with doxorubicin targeting nuclear factor kappaB-mediated inducible chemoresistance显示文摘 | Singh RP Mallikarjuna GU Sharma G Dhanalakshmi S Tyagi AK Chan DC AgarwalC Agarwal R | | 0，，： | 1 |
| 16 | Interdecadal unstationary relationship between NAO and east China’s summer precipitation patterns显示文摘 | Gu Wei Li Chongyin Li Weijing Zhou Wen L. Chan Johnny C | 2009 | Geophysical Research Letters2009，，13： | 1 |
| 17 | Functions of Mulberry in the Development of Ecological Animal Husbandry and Development Ideas显示文摘Ecological animal husbandry is an animal husbandry industry system integrating ecological benefit,economic benefit and social benefit.Breeding cycle is one of the main patterns of ecological animal husbandry development,but breeding cycle does not form a relatively closed cycle of endogenous resources.In recent years,forage value and ecological value of mulberry have made some progresses in different research areas and angles,but the thought and practice of both forage and ecological value of mulberry has not been systematically interpreted and researched.Based on the short board of animal husbandry development and the advantage of mulberry,animal husbandry and mulberry were complemented and fused,and relatively closed planting and raising ecological recycling pattern with mulberry as the axis was raised.Phased scientific research and production test data were obtained,and the problems and development suggestions were put forward in the following aspects: selection of mulberry varieties with both forage and ecological value,cost control of mulberry with both forage and ecological value,quality and safety control system of forage mulberry,orientation of feeding value of forage mulberry. | Zeng Xiu Wang Jieping Zhou Chan Lv Jinfeng Wang Haiyan Gu Shanlin Xiong Dingkui | 2017 | Animal Husbandry and Feed Science2017，9，6： | 1 |
| 18 | Nanoparticles in medicine:therapeutic applications and developments显示文摘 | Zhang L Gu F X Chan J M | 2008 | Clin Pharmacol Ther2008，83，5： | 1 |
| 19 | Comparison of bioleaching of heavy metals from sewage sludge using iron-and suffur-oxidizing bacteria显示文摘 | Chan L C Gu x Y Wang J W C | 2003 | Advances in Environmental Research2003，7，： | 1 |
| 20 | Molecular characterization of an additional shrimp hyperglycemic hormone: cDNA cloning, gene organization, expres sion and biological assay of recombinant proteins显示文摘 | Gu Peili Yu Keili Chan S M | 2000 | FEBS Letters2000，472，1： | 1 |
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