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| 1 | Varicella-zoster virus as a causative agent of acute retinal necrosis in younger patients显示文摘Background: Herpes virus is considered to be the pathogen of acute retinal necrosis (ARN) infection. Previous studies have that patients with ARN caused by the varicella-zoster virus (VZV) are often older, and patients with herpes simplex virus (HSV) induced ARN are considerably younger. However, in our clinical work, we find that VZV is also a pathogen in younger ARN patients. We, therefore, aimed to analyze the common etiology of younger ARN patients. Methods: A retrospective analysis was made of 20 eyes (18 patients) diagnosed as having ARN in the Department of Ophthalmology of Peking Union Medical College Hospital from 2014 to 2016. All patients were reviewed for demographic data, clinical course, clinical manifestations, time from onset to initial physician visit, duration of follow-up, visual acuity at both presentation and final visit, and treatment strategies. A paired t test was used to compare visual acuity between the presenting vision and those of final follow-up. Vitreous or aqueous specimens from 18 eyes of 18 patients were analyzed with multiplex polymerase chain reaction (mPCR)/quantitative PCR (qPCR) and xTAG-liquid chip technology (xTAG-LCT) to determine the causative virus of ARN. Results: Final best visual acuity (BCVA) improved significantly from 1.36±0.95 (median 20/400) to 0.95±0.82 (median 20/100)￠=2.714, P = 0.015) after systemic and intravitreal antiviral treatment combined with or without pars plana vitrectomy. PCR and xTAG-LCT results showed four of the five samples in the younger group (32.2±5.2 years) and 12 of the 13 samples in the senior group (53.6±4.9 years) were positive for VZV, and two of the five samples in the younger group were positive for HSV-1. Conclusions: This study demonstrates that VZV is also a common causative virus for ARN in younger patients. Considering this finding, a systemic antiviral treatment protocol should be immediately changed to intravenous ganciclovir when the patient does not respond to acyclovir before determining the causative virus, especially in younger patients. | Hai-Yan Xu Meng-Da Li Jun-Jie Ye Chan Zhao Yun-Tao Hu Yu Di | 2019 | Chinese Medical Journal2019，，6： | 5 |
| 2 | Oncogene GAEC1 regulates CAPN10 expression which predicts survival in esophageal squamous cell carcinoma显示文摘AIM: To identify the downstream regulated genes of GAEC1 oncogene in esophageal squamous cell carcinoma and their clinicopathological significance. METHODS: The anti-proliferative effect of knocking down the expression of GAEC1 oncogene was stud-ied by using the RNA interference (RNAi) approach through transfecting the GAEC1 -overexpressed esophageal carcinoma cell line KYSE150 with the pSilencer vector cloned with a GAEC1 -targeted sequence, followed by MTS cell proliferation assay and cell cycle analysis using flow cytometry. RNA was then extracted from the parental, pSilencer-GAEC1 -targeted sequence transfected and pSilencer negative control vector transfected KYSE150 cells for further analysis of different patterns in gene expression. Genes differentially expressed with suppressed GAEC1 expression were then determined using Human Genome U133 Plus 2.0 cDNA microarray analysis by comparing with the parental cells and normalized with the pSilencer negative control vector transfected cells. The most prominently regulated genes were then studied by immunohistochemical staining using tissue microarrays to determine their clinicopathological correlations in esophageal squamous cell carcinoma by statistical analyses. RESULTS: The RNAi approach of knocking down gene expression showed the effective suppression of GAEC1 expression in esophageal squamous cell carcinoma cell line KYSE150 that resulted in the inhibition of cell proliferation and increase of apoptotic population. cDNA microarray analysis for identifying differentially expressed genes detected the greatest levels of downregulation of calpain 10 (CAPN10 ) and upregulation of trinucleotide repeat containing 6C (TNRC6C ) transcripts when GAEC1 expression was suppressed. At the tissue level, the high level expression of calpain 10 protein was significantly associated with longer patient survival (month) of esophageal squamous cell carcinoma compared to the patients with low level of calpain 10 expression (37.73 ± 16.33 vs 12.62 ± 12.44, P = 0.032). No significant correction was observed among the TNRC6C protein expression level and the clinocopathologcial features of esophageal squamous cell carcinoma. CONCLUSION: GAEC1 regulates the expression of CAPN10 and TNRC6C downstream. Calpain 10 expression is a potential prognostic marker in patients with esophageal squamous cell carcinoma. | Dessy Chan Miriam Yuen-Tung Tsoi Christina Di Liu SauHing Chan Simon Ying-Kit Law Kwok-Wah Chan Yuen-Piu Chan Vinod Gopalan Alfred King-Yin Lam Johnny Cheuk-On Tang | 2013 | World Journal of Gastroenterology2013，19，18： | 2 |
| 3 | PIWIL1 governs the crosstalk of cancer cell metabolism and immunosuppressive microenvironment in hepatocellular carcinoma显示文摘Altered energy metabolism of cancer cells shapes the immune cell response in the tumor microenvironment that faclitates tumorprogression.Herein,we reported the novel of tumor cell-expressed Piwi Like RNA-Mediated Gene Silencing 1(PWL1)in mediatingthe crosstlk of fatty acid metabolism and immune response of human hepatocellular carcinoma(HCC).PlWIL1 expression in HCCwas increased compared to normal hepatic tissues and was positively correlated with the proliferation rate of HCC cell ines.PIWL1overexpression accelerated in vitro proliferation and in vivo growth of HCC tumors,while PIWL1 knockdown showed opositeeffects.PIWL1 increased oxygen consumption and energy production via fatty acid metabolism without altering aerobic glycolysis.lnhibition of fatty acid metablism abolished PWIL1-induced HCC prolferation and growth.RNA-seq analysis revealed that immunesystem regulation might be involved,which was echoed by the experimental observation that PIWL1-overexpressing HCC cellsattracted myeloid-derived suppressor cells(MDSCs)into the tumor microenvironment.MDSCs depletion reduced the prolferationand growth of PlWIL1-overexpressing HCC tumors.Complement C3,whose secretion was induced by PIWL1 in HCC cells,mediatesthe interaction of HCC cells with MDSCs by activated p38 MAPK signaling in MDSCs,which in turn initiated expression of immunosuppressive cytokine L10.Neutralizing lL10 secretion reduced the immunosuppressive activity of MDSCs in the microenvironment of PIWL1-overexpressing HCC.Taken together,our study unraveled the critical role of PIWL1 in initiating theinteraction of cancer cell metabolism and immune cell response in HCC.Tumor cell-expressed PlIWL1 may be a potential target forthe devellopment of novel HCC treatment. | Ning Wang Hor-Yue Tan Yuanjun Lu Yau-Tuen Chan Di Wang Wei Guo Yu Xu Cheng Zhang Feiyu Chen Guoyi Tang Yibin Feng | 2021 | Signal Transduction and Targeted Therapy2021，6，3： | 2 |
| 4 | Regulators of alternative polyadenylation operate at the transition from mitosis to meiosis显示文摘In the sexually reproductive organisms, gametes are produced by meiosis following a limited mitotic amplification. However, the intrinsic program switching cells from mitotic to meiotic cycle is unclear.Alternative polyadenylation(APA) is a highly conserved means of gene regulation and is achieved by the RNA 30-processing machinery to generate diverse 30 UTR profiles. In Drosophila spermatogenesis, we observed distinct profiles of transcriptome-wide 30 UTR between mitotic and meiotic cells. In mutant germ cells stuck in mitosis, 30 UTRs of hundreds of genes were consistently shifted. Remarkably, altering the levels of multiple 30-processing factors disrupted germline's progression to meiosis, indicative of APA's active role in this transition. An RNA-binding protein(RBP) Tut could directly bind 30 UTRs of 30-processing factors whose expressions were repressed in the presence of Tut-containing complex. Further,we demonstrated that this RBP complex could execute the repression post-transcriptionally by recruiting CCR4/Twin of deadenylation complex. Thus, we propose that an RBP complex regulates the dynamic APA profile to promote the mitosis-to-meiosis transition. | Lingjuan Shan Chan Wu Di Chen Lei Hou Xin Li Lixia Wang Xiao Chu Yifeng Hou Zhaohui Wang | 2017 | Journal of Genetics and Genomics2017，44，2： | 1 |
| 5 | Benefits of a pediatric an-timicrobial stewardship program at a children′s hospital显示文摘 | Di Pentima MC Chan S Hossain J | 2011 | Pediatrics2011，128，6： | 1 |
| 6 | Character ization and management of mandibular fractures,lesson learned form iraq and afghanistan显示文摘 | Tucker DI Zachar MR Chan R K | 2013 | Atlas Oral Maxillofar Surg Clin North Am2013，21，1： | 1 |
| 7 | Characterization of the Peri-Infarct Zone by Contrast-Enhanced Cardiac Magnetic Resonance Imaging Is a Powerful Predictor of Post–Myocardial Infarction Mortality显示文摘 | Andrew T. Yan Adolphe J. Shayne Kenneth A. Brown Sandeep N. Gupta Carmen W. Chan Tuan M. Luu Marcelo F. Di Carli H Glenn Reynolds William G. Stevenson Raymond Y. Kwong | 2006 | Circulation2006，，1： | 1 |
| 8 | Screening for Barrettes esophagus显示文摘 | di Pietro M Chan D Fitzgerald RC | 2015 | Gastroenterology2015，148，5： | 1 |
| 9 | Clinical pharmacogenetics and potential application in personalized medicine显示文摘 | ZHOU SF DI YM CHAN E | 2008 | Curr Drug Metab2008，9，8： | 1 |
| 10 | HER-2/neu as apredictive marker in a population of advanced breastcancer patients randomly treated either with single-agentdoxorubicin or single-agent docetaxel显示文摘 | Di Leo A Chan S Paesmans M | 2004 | Breast CancerRes Treat2004，86，3： | 1 |
| 11 | Tryptophan- and argi- nine-rich antimicrobial peptides: structures and mechanisms of action显示文摘 | Chan DI Prenner EJ Vogel HJ | 2006 | Biochim Biophys Acta2006，1758，9： | 1 |
| 12 | Induction of E-cadherin in lung cancer and interaction with growth suppression by histone deacetylase inhibition显示文摘 | Kakihana M Ohira T Chan Di | 2009 | J Thorac Oncol2009，4，12： | 1 |
| 13 | Clinical pharmacogenetics and potential application in personalized medicine显示文摘 | Zhou S F Di Y M Chan E | 2008 | Current Drug Metabolism2008，9，8： | 1 |
| 14 | Tryptophan- and arginine-rich antimicrobial peptides: Structures and mechanisms of action 显示文摘 | Chan DI Prenner EJ Vogel HJ | 2006 | BBA Biomembranes2006，1758，： | 1 |
| 15 | HER-2/neu as a predictive marker in a population of advanced breast cancer patients randomly treated either with single-agent doxorubicin or single-agent docetaxed显示文摘 | DI LEO A CHAN S PACSMANS M | 2004 | Breast Cancer Retreat2004，86，3： | 1 |
| 16 | Numerical simulation of atmospheric pollutant dispersion in an urban street canyon: Comparison between RANS and LES显示文摘 | Salim Mohamed Salim Riccardo Buccolieri Andrew Chan Silvana Di Sabatino | 2010 | Journal of Wind Engineering & Industrial Aerodynamics2010，，2： | 1 |
| 17 | Novel epigallocatechin gallate analogs as potential anticancer agents: a patent review (2009 – present)显示文摘 | Kristin Landis-Piwowar Di Chen Robert Foldes Tak-Hang Chan Qing Ping Dou | 2013 | Expert Opinion on Therapeutic Patents2013，，2： | 1 |
| 18 | Design and synthesis of delivery system based on SBA 15 with magnetic particles formed in situ and thermo- sensitive PNIPA as controlled switch显示文摘 | Zhu Shenmin Zhou Zhengyang Zhang Di Jin Chan Li Zhiqiang | 2007 | Microporous and Mesoporous Materials2007，106，123： | 1 |
| 19 | Fingerprint profiling of acidhydrolyzates of polysaccharides extracted from the fruiting bodies and spores of Lingzhi byhigh-performance thin-layer chromatography显示文摘 | Di X Chan K K Leungh W | 2003 | Journal of Chromatography A2003，1018，1： | 1 |
| 20 | Human macro-phage inflammatory protein 3alpha:protein and peptide nuclear magnetic resonance solution structures,dimerization,dynamics,and anti-infective properties显示文摘 | Chan DI Hunter HN Tack BF | 2008 | Antimicrob Agents Chemother2008，52，3： | 1 |
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