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| 1 | Bruggeman,and Cornelis Vink,The Epstein-Barr Virus BILF1 Gene Encodes a G Protein-Coupled Receptor That Inhibits Phosphorylation of RNA-Dependent Protein Kinase显示文摘 | Patrick S.Beisser Dennis Verzijl Yvonne K.Gruijthuijsen Erik Beuken Martine J.Smit.Rob Leurs Cathrien A | | 0，，： | 1 |
| 2 | Prevalence and resistance of commensal Staphylococcus aureus , including meticillin-resistant S aureus , in nine European countries: a cross-sectional study显示文摘 | Casper DJ den Heijer Evelien ME van Bijnen W John Paget Mike Pringle Herman Goossens Cathrien A Bruggeman Fran?ois G Schellevis Ellen E Stobberingh | 2013 | The Lancet Infectious Diseases2013，，5： | 1 |
| 3 | Tuf m RNA rather than 16S r RNA is associated with culturable Staphylococcus aureus显示文摘AIM: To study the presence of various nucleic acids targets of Staphylococcus aureus(S. aureus) during bacterial growth and antibiotic induced killing in relation to viability.METHODS: S. aureus was cultured to log phase and spiked in Todd Hewitt(TH) broth and whole blood of healthy human volunteers. Viability of S. aureus after flucloxacillin treatment(0, 1, 3 and 6 d) was assessed by culture on bloodagar plates. DNA and RNA were isolated from 200 μL. c DNA synthesis was performed by using random primers. The presence of S. aureus DNA, r RNA, and m RNA were determined by real-time polymerase chain reaction of the 16 S r DNA and tuf gene(elongation factor Tu).RESULTS: S. aureus spiked in TH broth without antibiotics grew from day 0-6 and DNA(tuf and 16S), and 16 S r RNA remained detectable during this whole period. During flucloxacillin treatment S. aureus lost viability from day 3 onwards, while the 16 S r RNA-gene and its RNA transcripts remained detectable. DNA andr RNA can be detected in flucloxacillin treated S. aureus cultures that do not further contain culturable bacteria.However, tuf m RNA became undetectable from day 3onwards. Tuf m RNA can only be detected from samples with culturable bacteria. When spiking S. aureus in whole blood instead of broth no bacterial growth was seen, neither in the absence nor in the presence of flucloxacillin. Accordingly, no increase in DNA and RNA levels of both 16 S r DNA and the tuf gene were detected. CONCLUSION: Tuf m RNA expression is associated with culturable S. aureus and might be used to monitor antibiotic effects. | Anne JM Loonen Petra FG Wolffs Maikel de Bresser Maurice Habraken Cathrien A Bruggeman Mirjam HA Hermans Adriaan JC van den Brule | 2015 | World Journal of Clinical Infectious Diseases2015，5，4： | 0 |
| 4 | 永远都是茶显示文摘Michelle,30岁,是个英国女孩,问她最喜欢的饮料,她回答“Tea forever(永远都是茶)”。和Michelle去郊游,下午3点她们准时拿出野餐篮喝茶,手里端着的杯碟都是一次性纸杯,至于曾经喝茶的那些昂贵易碎的骨瓷餐具,她们的妈妈早已将之摆在家里明亮的展示柜中了。 | Cathrien 小牧(图) | 2007 | 世界2007，，8： | 0 |
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