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| 1 | Liver fat deposition and mitochondrial dysfunction in morbid obesity:An approach combining metabolomics with liver imaging and histology显示文摘AIM: To explore the usefulness of magnetic resonance imaging(MRI) and spectroscopy(MRS) for assessment of non-alcoholic fat liver disease(NAFLD) as compared with liver histological and metabolomics findings. METHODS: Patients undergoing bariatric surgery following procedures involved in laparoscopic sleeve gastrectomy were recruited as a model of obesityinduced NAFLD in an observational, prospective, singlesite, cross-sectional study with a pre-set duration of 1 year. Relevant data were obtained prospectively and surrogates for inflammation, oxidative stress and lipid and glucose metabolism were obtained through standard laboratory measurements. To provide reliable data from MRI and MRS, novel procedures were designed to limit sampling variability and other sources of error using a 1.5T Signa HDx scanner and protocols acquired from the 3D or 2D Fat SAT FIESTA prescription manager. We used our previously described 1H NMRbased metabolomics assays. Data were obtained immediately before surgery and after a 12-mo period including histology of the liver and measurement of metabolites. Values from 1H NMR spectra obtained after surgery were omitted due to technical limitations.RESULTS: MRI data showed excellent correlation with the concentration of liver triglycerides, other hepatic lipid components and the histological assessment, w h i c h e xc l u d e d t h e p r e s e n c e o f n o n-a l c o h o l i c steatohepatitis(NASH). MRI was sufficient to follow up NAFLD in obese patients undergoing bariatric surgery and data suggest usefulness in other clinical situations. The information provided by MRS replicated that obtained by MRI using the-CH3 peak(0.9 ppm), the-CH2- peak(1.3 ppm, mostly triglyceride) and the-CH=CH- peak(2.2 ppm). No patient depicted NASH. After surgery all patients significantly decreased their body weight and steatosis was virtually absent even in patients with previous severe disease. Improvement was also observed in the serum concentrations of selected variables. The most relevant findings using metabolomics indicate increased levels of triglyceride and monounsaturated fatty acids in severe steatosis but those results were accompanied by a significant depletion of diglycerides, polyunsaturated fatty acids, glucose-6-phosphate and the ATP/AMP ratio. Combined data indicated the coordinated action on mitochondrial fat oxidation and glucose transport activity and may support the consideration of NAFLD as a likely mitochondrial disease. This concept may helpto explain the dissociation between excess lipid storage in adipose tissue and NAFLD and may direct the search for plasma biomarkers and novel therapeutic strategies. A limitation of our study is that data were obtained in a relatively low number of patients.CONCLUSION: MRI is sufficient to stage NAFLD in obese patients and to assess the improvement after bariatric surgery. Other data were superfluous for this purpose. | Nahum Calvo Raúl Beltrán-Debón Esther Rodríguez-Gallego Anna Hernández-Aguilera Maria Guirro Roger Mariné-Casadó Lidón Millá Josep M Alegret Fàtima Sabench Daniel del Castillo María Vinaixa Miguelàngel Rodríguez Xavier Correig Roberto García-álvarez Javier A Menendez Jordi Camps Jorge Joven | 2015 | World Journal of Gastroenterology2015，21，24： | 2 |
| 2 | Herbicide resistant fertile transgenic wheat plant obtained by microprojectile bombardment of regenerable embryogenic callus显示文摘 | Vasil V Castillo A M Fromm M E Vasil I K | 1992 | Bio/Tech1992，10，： | 2 |
| 3 | Genotypic variation in nitrogen use efficiency in medium- and long-duration rice显示文摘 | U Singh J.K Ladha E.G Castillo G Punzalan A Tirol-Padre M Duqueza | 1998 | Field Crops Research1998，，1： | 2 |
| 4 | Aqueous extract of Ocimum tenuiflorum decreases levels of blood glucose in induced hyperglycemic tilapia(Oreochromis niloticus)显示文摘Objective:To evaluate,in hyperglycemic tilapia[Oreochromis niloticus(O.niloticus)].the effect of this aqueous extract on blood glucose levels.Methods:The hyperglycemia in O.niloticus was induced by adding glucose to fish pond water.An aqueous extract of Ocimum tenuiflorum (O.tenuiflorum) was prepared by boiling fresh leaves and the doses of 0,40,80,200 and 400 mg per liter of pond water were tested.Results:The blood sugar concentration for tilapia with hyperglycemic induced was an average of 50%higher than the control group.The blood glucose levels in tilapia after the induction of hyperglycemia were higher than the control group for 90 min after the treatment.The treatment with the aqueous extract of O.tenuiflorum dropped the serum glucose level of hyperglycemic tilapia until it was similar to that of the control group and was dose dependent.Conclusions:The results indicated that O.tenuiflorum was endowed with anti-hyperglycemic activity.To our knowledge,this is the first report on the use of fish as a diabetes model to test natural extracts from plants. | Amilcar Arenal Leonardo Martín Nestor M Castillo Dainier de la Torre Ubaldo Torres Reinaldo Gonzlez | 2012 | Asian Pacific Journal of Tropical Medicine2012，5，8： | 2 |
| 5 | Decontamination of beef carcass surface tissue by steam vacuuming alone and combined wit h hot water and Iactic acid sprays显示文摘 | Castillo A L ucia L M Goodson K J | 1999 | J Food Prot1999，62，： | 1 |
| 6 | An Assessment of Ad- vanced Mobile Services Acceptance: Contributions from TAM and Diffusion Theory Models 显示文摘 | Nicolas C L Castillo F J M Bouwman H | 2008 | Information & Management2008，45，6： | 1 |
| 7 | Clinical applications of proton MR spectroscopy显示文摘 | Castillo M Kwock L Mukherji SK | 1996 | AJNR1996，17，1： | 1 |
| 8 | The release of tumor necrosis factor-alpha is associated with ische mic tolerance in human stroke显示文摘 | Castillo J Moro MA Blanco M | 2003 | Ann Neurol2003，54，6： | 1 |
| 9 | The release of tumor necrosis factor-alpha is associated with ischemic tolerance in human stroke显示文摘 | Castillo J Moro MA Blanco M | 2003 | Ann Neurol2003，54，6： | 1 |
| 10 | Apparent diffusion coefficients in the evaluation of high-grade cerebral gliomas显示文摘 | Castillo M Smith JK Kwock L | 2001 | AJNR Am J Neuroradiol2001，22，1： | 1 |
| 11 | Paraoxonase 2 polymorphisms are associated with nephropathy in Type Ⅱ diabetes显示文摘 | Pinizzotto M Castillo E Temler E | 2001 | Diabetologia2001，44，： | 1 |
| 12 | Somatic embryogenesis and plant regenerarion from barley cuhivars grown in spain 显示文摘 | Castillo A Egana B Sanz J M | 1995 | Plant Cell Rep1995，17，： | 1 |
| 13 | Setup ad- justment of multiple lots using a sequential monte carlo meth- od显示文摘 | LIAN Z COLSIMO B M DEL CASTILLO E | 2006 | Technometries2006，48，3： | 1 |
| 14 | Bayesian modelingand optimization of functional responses affected by noise factors显示文摘 | Del Castillo E Colosimo B M Alshraideh H | 2012 | Journal of Quality Technology2012，44，2： | 1 |
| 15 | Correlation of Myoinositol Levels and Grading of Cerebral Astroeytomas显示文摘 | Castillo M Smith JK Kwoch I | 2000 | AJNR2000，21，11： | 1 |
| 16 | A blast of gas in the latest Paleocene: simulation first-order effects of massive dissociation of oceanic methane hydrate显示文摘 | Castillo M M Walker J G C | 1997 | Geology1997，25，： | 1 |
| 17 | A Bayesian approach for multiple response surface optimization in the presence of noise variables显示文摘 | Castillo E D Peterson J J | 2004 | J Applied Statistics2004，31，3： | 1 |
| 18 | Impact of hypercalcemia and parathyroid hormone level on the sensitivity of preoperative sestamibi scanning for primary hyperparathyroidism显示文摘 | Parikshak M Castillo E D Conrad M F | 2003 | Am Surg2003，69，5： | 1 |
| 19 | Solid-phase microcxtraction as a methodology in the identification of irradiated ground beef显示文摘 | Caja M M Castillo M L R Blanch G P | 2008 | Food Chemistry2008，110，2： | 1 |
| 20 | Isolation and characterization of PRA1,a trypsin -like protease from the biocontrol agent Trichoderma har-zianum CECT 2413 displaying nematicidal activity显示文摘 | SUAREZ B REY M CASTILLO P | 2004 | Appl Microbiol Bio-technol2004，，： | 1 |
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