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| 1 | Hepatogenic differentiation of human mesenchymal stem cells from adipose tissue in comparison with bone marrow mesenchymal stem cells显示文摘AIM: To investigate and compare the hepatogenic transdifferentiation of adipose tissue-derived stem cells (ADSC) and bone marrow-derived mesenchymal stem cells (BMSC) in vitro. Transdifferentiation of BMSC into hepatic cells in vivo has been described. Adipose tissue represents an accessible source of ADSC, with similar characteristics to BMSC.METHODS: BMSCs were obtained from patients under-going total hip arthroplasty and ADSC from human adi-pose tissue obtained from lipectomy. Cells were grown in medium containing 15% human serum. Cultures were serum deprived for 2 d before cultivating under similar pro-hepatogenic conditions to those of liver develop-ment using a 2-step protocol with sequential addition of growth factors, cytokines and hormones. Hepatic differ-entiation was RT-PCR-assessed and liver-marker genes were immunohistochemically analysed. RESULTS: BMSC and ADSC exhibited a fibroblastic mor-phology that changed to a polygonal shape when cells differentiated. Expression of stem cell marker Thy1 de-creased in differentiated ADSC and BMSC. However, the expression of the hepatic markers, albumin and CYPs increased to a similar extent in differentiated BMSC and ADSC. Hepatic gene activation could be attributed to in-creased liver-enriched transcription factors (C/EBPβ and HNF4α), as demonstrated by adenoviral expression vec-tors.CONCLUSION: Mesenchymal stem cells can be induced to hepatogenic transdifferentiation in vitro. ADSCs have a similar hepatogenic differentiation potential to BMSC, but a longer culture period and higher proliferation ca-pacity. Therefore, adipose tissue may be an ideal source of large amounts of autologous stem cells, and may become an alternative for hepatocyte regeneration, liver cell transplantation or preclinical drug testing. | Raquel Taléns-Visconti Ana Bonora Ramiro Jover Vicente Mirabet Francisco Carbonell José Vicente Castell María José Gómez-Lechón | 2006 | World Journal of Gastroenterology2006，12，36： | 35 |
| 2 | Hepatocyte transplantation program:Lessons learned andfuture strategies显示文摘This review aims to share the lessons we learned over time during the setting of the hepatocyte transplantation(HT) program at the Hepatic Cell Therapy Unit at Hospital La Fe in Valencia. New sources of liver tissue for hepatocyte isolation have been explored. The hepatocyte isolation and cryopreservation procedures have been optimized and quality criteria for assessment of functionality of hepatocyte preparations and suitability for HT have been established. The results indicate that:(1) Only highly viable and functional hepatocytes allow to recover those functions lacking in the native liver;(2) Organs with steatosis(≥ 40%) and from elderly donors are declined since low hepatocyte yields, viability and cell survival after cryopreservation, are obtained;(3) Neonatal hepatocytes are cryopreserved without significant loss of viability or function representing high-quality cells to improve human HT;(4) Cryopreservation has the advantage of providing hepatocytes constantly available and of allowing the quality evaluation and suitability for transplantation; and(5) Our results from 5 adults with acute liver failure and 4 from children with inborn metabolic diseases, indicate that HT could be a veryuseful and safe cell therapy, as long as viable and metabolically functional human hepatocytes are used. | Eugenia Pareja Ibars Miriam Cortes Laia Tolosa Maria JoséGómez-Lechón Slivia López JoséVicente Castell JoséMir | 2016 | World Journal of Gastroenterology2016，22，2： | 3 |
| 3 | Cardiac alterations in cirrhosis: reversibility after liver transplantation显示文摘 | Mireia Torregrosa Santi Aguadé Laura Dos Rosa Segura Antonio Gónzalez Artur Evangelista Joan Castell Carlos Margarit Rafael Esteban Jaume Guardia Joan Genescà | 2004 | Journal of Hepatology2004，，1： | 3 |
| 4 | Effects of purified dies containing different combinations of arachidonic and docosahexaenoic acid on survival, growth and fatty acid composition of juvenile turbot (Scophthalmus maximus) 显示文摘 | Castell J D Bell J G Tocher D R | 1994 | Aquaculture1994，1，： | 2 |
| 5 | Mechanical behavior model of corroded reinforced concrete 显示文摘 | Castel A Franqois R Arliguie G | 2002 | Comptes Rendus de 1'' Acad6mie des Sciences-S6rie IIb/Me- chanics2002，，330： | 1 |
| 6 | Operational Energy in the Life Cycle of Residential Dwellings: The Experience of Spain and Colombia 显示文摘 | Ortiz O Castells F Sonnemann G | 2010 | Applied Energy2010，，87： | 1 |
| 7 | Effects of purified diets containing different combinations of arachidonic and docosahexaenoic acid on survival,growth and fatty acid composition of juvenile turbot (Scophthalmus maximus )显示文摘 | Castell J D Bell J G Tocher D R | 1994 | Aquaculture1994，128，： | 1 |
| 8 | Discrimination of pathogenic clinical isolates and laboratorystrains of Bacillus cereus by NMR-based metabolomicsprofiling 显示文摘 | BUNDY J G WILLEY T L CASTELL R S | 2005 | FEMS Microbiol Lett2005，242，1： | 1 |
| 9 | Culture of human hepatocytes from small surgical liver biopsies. Biochemical characterization and comparison with in vivo显示文摘 | M. José Gómez-Lechón Pilar López Teresa Donato Angel Montoya Amparo Larrauri Patricia Giménez Ramón Trullenque Ricardo Fabra José V. Castell | 1990 | In Vitro Cellular & Developmental Biology1990，，1： | 1 |
| 10 | Thiamphenicol disposition in pigs 显示文摘 | Castells G Prats C El Korchi G | 1999 | Res Vet Sci1999，66，03： | 1 |
| 11 | Performance,carcass and pork characteristics of castrates and gills self-fed diets differing in protein content and lysine:energy ratio显示文摘 | CASTELL A G CIIPLET R L POSTE-FLYNN L M | 1994 | Can J Anim Sci1994，74，3： | 1 |
| 12 | Influence of the novel inotropic agent levosimendan on isometric tension and calcium cycling in failing human myocardium显示文摘 | HASENFUSS G PIESKE B CASTELL M | 1998 | Circulation1998，98，20： | 1 |
| 13 | Sustainability in the construction industry:A review of recent developments based on LCA显示文摘 | O Ortiz F Castells G Sonnemann | | 0，，： | 1 |
| 14 | Enhanced steatosis by nuclear receptor ligands: a study in cultured human hepatocytes and hepatoma cells with a characterized nuclear receptor expression profile显示文摘 | Moya M Gómez-Lechón MJ Castell JV Jover R | 2010 | Chem Biol Interact2010，184，3： | 1 |
| 15 | Influence if the novel inotropic a- gent levosimendan on isometric tension and calcium cycling in failing hu- man myocardium 显示文摘 | Hasenfuss G Pieske B Castell M | 1998 | Circulation1998，98，20： | 1 |
| 16 | Utilization of the energy in ocean waves 显示文摘 | Isaacs J D Castel D Wick G L | 1976 | Ocean Engineering1976，3，4： | 1 |
| 17 | Effect of loading on carbonation penetration in reinforced concrete elements显示文摘 | Castel A Francccois R Arliguie G | 1999 | Cement and Concrete Research1999，29，4： | 1 |
| 18 | Effect of loading on carbonation penetration in reinforced concrete elements显示文摘 | CASTEL A FRANCOIS R ARLIGUIE G | 1999 | Cement and Concrete Research1999，29，4： | 1 |
| 19 | Sysetemic lupus erythematosus 显示文摘 | Ruiz-lrastorza G Khamashta MA Castel lino | 2001 | Lancet2001，357，9261： | 1 |
| 20 | Effect of loading on carbonation penetration in reinforced concrete elements显示文摘 | CASTEL A FRANCOIS R ARLIGUIE G | | 0，，04： | 1 |
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