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| 1 | Multispecies probiotic protects gut barrier function in experimental models显示文摘AIM:To investigate the effect of the probiotic combination Lactibiane Tolerance?(LT)on epithelial barrier function in vitro and in vivo.METHODS:The effect of the multispecies probiotic LT was assessed on several models of epithelial barrier function both in vitro(in basal and inflammatory conditions)and in vivo[visceral hypersensitivity induced by chronic stress or by colonic perfusion of a fecal supernatant(FSN)from patients with irritable bowel syndrome(IBS)].In vitro,we measured the permeability of confluent T84 cell monolayers incubated with or without LT by evaluating the paracellular flux of macromolecules,in basal conditions and after stimulation with lipopolysaccharide(LPS)or with conditioned medium of colonic biopsies from IBS patients(IBS-CM).In vivo,male C57/Bl6 mice received orally NaCl or LT for 15 d and were submitted to water avoidance stress(WAS)before evaluating visceral sensitivity by measuring the myoelectrical activity of the abdominal muscle and the paracellular permeability with 51Cr-EDTA.Permeability and sensitivity were also measured after colonic instillation of FSN.Tight-junctions were assessed by immunoblotting and TLR-4 expression was evaluated by immunohistochemistry RESULTS:Incubation of T84 cell monolayers with LT in basal conditions had no significant effect on permeability(P>0.05 vs culture medium).By contrast,addition of LT bacterial bodies(LT)completely prevented the LPS-induced increase in paracellular permeability(P<0.01 vs LPS 10 ng/mL(LPS 10);P<0.01 vs LPS 100ng/mL(LPS 100),P>0.05 vs culture medium).The effect was dose dependent as addition of 109 LT bacterial bodies induced a stronger decrease in absorbance than 106 LT(109 LT+LPS 10:-20.1%±13.4,P<0.01vs LPS 10;106 LT+LPS 10:-11.6%±6.2,P<0.01 vs LPS 10;109 LT+LPS 100:-14.4%±5.5,P<0.01 vs LPS 100;106 LT+LPS 100:-11.6%±7.3,P<0.05 vs LPS 100).Moreover,the increase in paracellular permeability induced by culturing T84 cells with conditioned medium of colonic biopsies from IBS patients(IBS-CM)was completely inhibited in the presence of 109 LT(P<0.01 vs IBS-CM).LT also significantly prevented the epithelial disruption induced by intracolonic infusion of fecal supernatant from IBS patients(P<0.01 vs IBS FSN)or water avoidance stress P<0.01 vs WAS)in C57/Bl6 mice and increased the expression of occludin in vitro and in vivo,as assessed by immnunoblotting.The WAS-induced effect on visceral sensitivity was prevented by LT treatment since values obtained for all steps of colorectal distension were significantly(P<0.01)different from the WAS group.Finally,LT downregulated the response mediated through TLR-4 in vitro(decrease in tumor necrosis factorαsecretion in response to LPS:-65.8%for 109 LT and-52.5%for 106LT,P<0.01 vs LPS)and in vivo(inhibition of WAS induced an increase in TLR-4 expression in the LT treated mice colon,P<0.01 vs WAS).CONCLUSION:The probiotic LT mix prevented the disruption to the epithelial barrier induced by LPS,stress or colonic soluble factors from IBS patients and prevented visceral hypersensitivity. | Mylene Nébot-Vivinus Cherryl Harkat Hanene Bzioueche Christel Cartier Raffaella Plichon-Dainese Lara Moussa Helene Eutamene Dorsa Pishvaie Sophie Holowacz Christian Seyrig Thierry Piche Vassilia Theodorou | 2014 | World Journal of Gastroenterology2014，20，22： | 12 |
| 2 | Addition of statins to the standard treatment in patients with cirrhosis:Safety and efficacy显示文摘This review summarizes the safety and efficacy of statins in patients with cirrhosis.Due to concerns about the safety of statins in patients with impaired liver function,they have recently been investigated as a potential treatment option in cirrhosis.The most clinically significant adverse event is statin-related myopathy,and this may be related to the high serum statin concentrations in the setting of severely impaired liver function.Rhabdomyolysis is the most serious and potentially life-threatening manifestation.It has recently been demonstrated that the recommended dose of simvastatin in patients with decompensated cirrhosis would be 20 mg/d because higher values,such as 40 mg/d,are associated with many adverse events,especially muscle injury.Likewise,simvastatin should not be administered to patients with Model for End-stage Liver Disease score>12 and/or Child-Pugh class C because of the high risk of severe muscle injury.Due to the pleiotropic effects,the focus on statins has shifted from being considered harmful to something useful.Through these effects,statins could prevent liver-related morbidity and mortality in cirrhotic patients.Observational studies in large populations of patients with cirrhosis have shown that treatment with statins to decrease high cholesterol levels was associated with a reduced risk of hepatic decompensation,hepatocellular carcinoma development and death.The few randomized controlled trials in patients with cirrhosis and portal hypertension showed that statins lower portal pressure,quite likely through a reduction in hepatic resistance.Another large randomized controlled trial in patients with variceal bleeding showed that simvastatin in addition to standard of care did not prevent rebleeding but improved survival rate.Despite these encouraging outcomes,the quality of the evidence regarding the use of statins is low or very low due to the observational characteristics of most of the studies involved.Therefore,it is advisable to perform further randomized controlled trials on a large series of patients with hard clinical endpoints,using different statin types and varying doses.The objectives would be to prevent liver-related morbidity and mortality rather than treating cirrhosis complications to take additional information that makes it possible to add statins to the standard of care of these patients. | Alberto E Muñoz Florencia D Pollarsky Mónica Marino Mariano Cartier Horacio Vázquez Pablo Salgado Gustavo Romero | 2021 | World Journal of Gastroenterology2021，27，28： | 4 |
| 3 | Prevention of gut leakiness by a probiotic treatment leads to attenuated HPA response to an acute psychological stress in rats显示文摘 | Afifa Ait-Belgnaoui Henri Durand Christel Cartier Gilles Chaumaz Hélène Eutamene Laurent Ferrier Eric Houdeau Jean Fioramonti Lionel Bueno Vassilia Theodorou | 2012 | Psychoneuroendocrinology2012，，11： | 2 |
| 4 | Origin of the threshold voltage instability in SiO2/HfO2 dual layer gate dielectrics 显示文摘 | KERBER A CARTIER E PANTISANO L | 2003 | IEEE EDL2003，24，2： | 1 |
| 5 | Kinetics of the in situ polymerization and in situ compatibilization of poly(propylene) and polyamide 6 blends显示文摘 | Guo-Hua Hu Herve Cartier Lian-Fang Feng | 2004 | J Appl Polym Sci2004，91，： | 1 |
| 6 | Systematic off-pump coronary artery revascularization: experience of 275 cases 显示文摘 | CARTIER R | 1999 | Ann Thorac Surg1999，68，4： | 1 |
| 7 | Urinary leukotriene F4 levels during early and late asthmatic responses 显示文摘 | Manning PJ Rokach J Malo JL Ethier D Cartier A Girard Y Charleson S O'Byrne PM | 1990 | J Allergy Clin Immunol1990，86，2： | 1 |
| 8 | Styrene-assisted melt free radical grafting of glycidyl methacrylate onto polypropylene显示文摘 | Cartier H Hu Guo-hua | 1997 | J Polym Sci A:Polym Chem1997，36，： | 1 |
| 9 | The use of antiplatelet therapy in the outpatient setting: Canadian Cardiovascular Society guidelines 显示文摘 | Bell AD Roussin A Cartier R | 2011 | Can J Cardiol2011，27，： | 1 |
| 10 | Systematic review:the Trousseau syndrome revisited:should we screen extensively for cancer in patients with venous thromboembolism?显示文摘 | Cartier M Le Gai G Wells PS | 2008 | Ann Intern Med2008，149，： | 1 |
| 11 | Nosiheptide,a sulfur-containing peptide antibiotic isolated from Strep40037显示文摘 | BANAZET F CARTIER M FLORENT J | 1980 | Experientia1980，36，： | 1 |
| 12 | Styrene- Assisted Free Radical Grafting of Glycidyl Methacrylate onto Polyethylene in the Melt显示文摘 | H CARTIER G H HU | 1998 | J Polym Sci:Part A:Polym Chem1998，36，： | 1 |
| 13 | Age-related differences in inflammatory markers in men:contribution of visceral adiposity显示文摘 | Cartier A Cote M | 2009 | Metabolism2009，58，10： | 1 |
| 14 | Age-related differences in inflammatory markers in men: contribution of visceral adiposity显示文摘 | Cartier A CSt 6 M Lemieux I | 2009 | Metabolism2009，58，10： | 1 |
| 15 | Styrene-assisted melt free radical grafting of glycidyl methacrylate onto polypropylene显示文摘 | Hu G H | 1998 | Journal of Polymer Science:Part A:Polymer Chemistry1998，36，： | 1 |
| 16 | Quercintrin 3'-sulphate from leaves of Leea guinensis显示文摘 | Beck P D D B Dijoux M G Cartier G | 1998 | Phytochemistry1998，47，6： | 1 |
| 17 | Epidemiological futures of HTLV - I and adult T cell leukemia显示文摘 | Tajima K Cartier L | 1995 | Intervirology1995，38，3： | 1 |
| 18 | Colonic luminal proteases activate colonocyte proteinase‐activated receptor‐2 and regulate paracellular permeability in mice显示文摘 | R.Róka J.Demaude N.Cenac L.Ferrier C.Salvador‐cartier R.Garcia‐villar J.Fioramonti L.Bueno | 2006 | Neurogastroenterology & Motility2006，，1： | 1 |
| 19 | The Us3 protein kinase of herpes simplex virus 1 blocks apoptosis and induces phosporylation of the Bc1-2 family member Bad显示文摘 | Cartier A Komai T Masucci M G | | 0，，01： | 1 |
| 20 | Prevention of gut leakiness by a probiotic treatment leads to attenuated HPA response to an acute psychological stress in rats显示文摘 | Ait-Belgnaoui A Durand H Cartier C | | 0，，： | 1 |
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