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| 1 | Isolation and diagnosis of Helicobacter pylori by a new method: Microcapillary culture显示文摘AIM:To investigate the performance of the microcapillary culture method(MCM) in Helicobacter pylori(H.pylori) isolation and diagnosis.METHODS:Microcapillary culture(MC),classical culture(CC),rapid urease(CLO) test,and histopathologic examination(HE) were performed with biopsy samples.Homogenized biopsy samples were loaded into capillary tubes and incubated for 48 h at 37 ℃ without providing a microaerophilic environment.Additionally,three or four loops of the homogenized sample were inoculated in a ready-to-use selective medium(Becton Dickinson,Helicobacter Agar,Modified) specific for the isolation of H.pylori and incubated at 37 ℃ in a microaerophilic atmosphere provided by Campy Gen(Becton Dickinson,Gas Pack).Bacteria reproducing in microcapillary tubes were evaluated in an inverted microscope and also were evaluated after performing a CC with the content.Results obtained by CC,CLO test,and HE were compared with those of MC.The diagnostic performances of the methods used in this study were evaluated for specificity,sensitivity,positive predictive value(PPV),negative predictive value(NPV),and CI.RESULTS:H.pylori was found positive by CLO test +HE and/or CC culture in 26 patient antrum and corpus biopsy samples.In 25(25/26) patient biopsy samples,H.pylori was isolated by MCM,whereas in only 14(14/26) patient biopsy samples,H.pylori was isolatedby CC.CLO test and HE were found positive in 17(17/26) patient biopsy samples.Comparing the results of the isolation of H.pylori by MCM,CC,CLO test,and HE,the sensitivity of the MCM was found as 96%,the specificity as 80%,the PPV as 83%,the NPV as 95%,and the 95%CI as 0.76(χ2 =31.51,P < 0.01) whereas the sensitivity of the CC was found as 54%(χ2 =19.15,P < 0.01),and the sensitivity of the CLO test and HE were found as 65%(χ2 =25.26,P < 0.01).CONCLUSION:This new microcapillary cultivation method for H.pylori has high diagnostic sensitivity compared with CC,HE,and CLO tests. | Adil M Allahverdiyev Melahat Bagirova Reyhan Caliskan Hrisi Bahar Tokman Hayat Aliyeva Gokce Unal Olga Nehir Oztel Emrah Sefik Abamor Hilal Toptas Pelin Yuksel Fatma Kalayci Mustafa Aslan Yusuf Erzin Kadir Bal Bekir S Kocazeybek | 2015 | World Journal of Gastroenterology2015，21，9： | 2 |
| 2 | The influence of circadian blood pressure changes on aortic distensibility and left ventricular diastolic function in hypertensive individuals显示文摘 | Erdogan D Gullu H Caliskan M etal | 2006 | Int J Cardiovasc Imaging2006，22，2： | 1 |
| 3 | Effects of atrovastatin on coronary flow reserve in patients with slow coronary flow显示文摘 | Caliskan M Erdogan D Gullu H | 2007 | Clin Cardiol2007，30，9： | 1 |
| 4 | Energy absorption and damage propagation in 2D triaxially braided carbon fiber composites: Effects of in situ matrix properties显示文摘 | Salvi A G Waas A M Caliskan A | 2008 | Journal of Materials Science2008，43，： | 1 |
| 5 | Effects of atrovastatin on coro- nary flow reserve in patients with slow coronary flow 显示文摘 | Caliskan M Erdogan D Gullu H | 2007 | Clin Cardiol2007，30，9： | 1 |
| 6 | The influence of circadi- an blood pressure changes on aortic distensibility and left ventric- ular diastolic function in hypertensive individuals显示文摘 | Erdogan D Gullu H Caliskan M | 2006 | Int J Cardio- vasc Imaging2006，22，2： | 1 |
| 7 | Relationship of serum uric to measures of endothelial function and athero- sclerosis in healthy adults显示文摘 | Erdogan D Gullu H Caliskan M | 2005 | Int J Clin Pract2005，59，11： | 1 |
| 8 | Coronary flow reserve and coronary microvascular functions are strongly related to serum uric acid concentrations in healthy adults显示文摘 | Erdogan D Gullu H Caliskan M | 2006 | Coron Artery Dis2006，17，： | 1 |
| 9 | Coronary flow reserve is impaired in patients with slow coronary flow 显示文摘 | Erdogan D Caliskan M Gullu H | 2007 | Atheroselerosis2007，191，1： | 1 |
| 10 | Role of prophylactic ipsilateral central compartment lymph node dissection in papillary thyroidmicrocarcinoma显示文摘 | CALISKAN M PARK J H JEONG J S | 2012 | Endocr J2012，59，： | 1 |
| 11 | Rhinovirus wheezing illness and genetic risk of childhood-onset asthma显示文摘 | Caliskan M Bochkov YA Kreiner-Mφller E | | 0，，15： | 1 |
| 12 | Significant correlation between uric acid levels and flow - mediated dilatation in patients with masked hypertension显示文摘 | Sincer II Kurtoglu E Caliskan M | 2014 | Clin Exp Hypertens2014，36，5： | 1 |
| 13 | Rhinovirus wheezing illness and genetic risk of childhood-onset asthma显示文摘 | Caliskan M Bochkov YA Kreiner-Mller E | 2013 | N Engl J Med2013，368，15： | 1 |
| 14 | Metformin therapy improves ovulatory rates, cervical scores, and pregnancy rates in clomiphene citrate-resistant women with polycystic ovary syndrome显示文摘 | Müberra Kocak Eray Caliskan Coskun Simsir Ali Haberal | 2002 | Fertility and Sterility2002，，1： | 1 |
| 15 | Experimental observation of left - handed transmission in a bilayer metamaterial under normal - to - plane propagation 显示文摘 | K Guven M D Caliskan and E Ozbay | 2006 | Optical Express2006，14，19： | 1 |
| 16 | Coronary flow reserve is impaired in patients with slow coronary flow 显示文摘 | Erdogan D Caliskan M Gullu H | 2007 | Atherosclerosis2007，191，1： | 1 |
| 17 | Congenital muscu- lar torticollis : evaluation and classification 显示文摘 | Tatli B Aydinli N Caliskan M | 2006 | Pediatr Neuro12006，34，1： | 1 |
| 18 | Reversible EEC changes during rotavirus gastroenteritis显示文摘 | Isik U Caliskan M | | 0，，01： | 1 |
| 19 | Metformin therapy improves ovu-latory rates,cervical scores,and pregnancy rates in clomiphene cit-rate-resistant women with polycystic ovary syndrome 显示文摘 | Kocak M Caliskan E Simsir C | 2002 | Fertility andSterility2002，77，1： | 1 |
| 20 | Uncontrolled generator operation of interior PM synchronous machines following high-speed inverter shutdown显示文摘 | Jahns T M Caliskan V | 1999 | IEEE Trans Ind Appl1999，35，6： | 1 |
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