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| 1 | Bone-conditioned medium contributes to initiation and progression of osteogenesis by exhibiting synergistic TGF-β1/BMP-2 activity显示文摘Guided bone regeneration (GBR) often utilizes a combination of autologous bone grafts, deproteinized bovine bone mineral(DBBM), and collagen membranes. DBBM and collagen membranes pre-coated with bone-conditioned medium (BCM) extracted from locally harvested autologous bone chips have shown great regenerative potential in GBR. However, the underlying molecular mechanism remains largely unknown. Here, we investigated the composition of BCM and its activity on the osteogenic potential of mesenchymal stromal cells. We detected a fast and significant (P <0.001) release of transforming growth factor-β1 (TGF-β1) from autologous bone within 10 min versus a delayed bone morphogenetic protein-2 (BMP-2) release from 40 min onwards. BCMs harvested within short time periods (10, 20, or 40 min), corresponding to the time of a typical surgical procedure, significantly increased the proliferative activity and collagen matrix production of BCM-treated cells. Long-term (1, 3, or 6 days)-extracted BCMs promoted the later stages of osteoblast differentiation and maturation. Short-term-extracted BCMs, in which TGF-β1 but no BMP-2was detected, reduced the expression of the late differentiation marker osteocalcin. However, when both growth factors were present simultaneously in the BCM, no inhibitory effects on osteoblast differentiation were observed, suggesting a synergistic TGF-β1/BMP-2 activity. Consequently, in cells that were co-stimulated with recombinant TGF-β1 and BMP-2, we showed a significant stimulatory and dose-dependent effect of TGF-β1 on BMP-2-induced osteoblast differentiation due to prolonged BMP signaling and reduced expression of the BMP-2 antagonist noggin. Altogether, our data provide new insights into the molecular mechanisms underlying the favorable outcome from GBR procedures using BCM, derived from autologous bone grafts. | Maria B.Asparuhova Jordi Caballé-Serrano Daniel Buser Vivianne Chappuis | 2018 | International Journal of Oral Science2018，10，4： | 17 |
| 2 | Hepatic Expression of CXC Chemokines Predicts Portal Hypertension and Survival in Patients With Alcoholic Hepatitis显示文摘 | Marlene Dominguez Rosa Miquel Jordi Colmenero Montserrat Moreno Joan–Carles García–Pagán Jaime Bosch Vicente Arroyo Pere Ginès Juan Caballería Ramón Bataller | 2009 | Gastroenterology2009，，5： | 3 |
| 3 | Avascular necrosis of the femoral head after femoral neck fracture显示文摘 | Caballer AP Portal L F | 2002 | Clin Orthop2002，39，9： | 1 |
| 4 | Solid-phase enzymelinked immunosorbent assay for hepatitis E virus IgG and IgM antibodles utilizing recombinant antigens and synthetic peptides 显示文摘 | Dawson GJ Chau KH Cabal CM | 1992 | J Virol Methods1992，38，1： | 1 |
| 5 | Avascalar necrosis of the femoral head after femoral neck fracture显示文摘 | Bachiller FG Caballer AP Portal LF | 2002 | Clin Orthop2002，，399： | 1 |
| 6 | A vascalar necrosis of the femoral head after femoral neck fracture显示文摘 | Caballer AP Portal LF | 2002 | Clin Orthop2002，，399： | 1 |
| 7 | Kinetics of naphthalene adsorption on an activated carbon: comparison between aqueous and organic media 显示文摘 | Cabal B Ania C O Parra J B | 2009 | Chemosphere2009，74，4： | 1 |
| 8 | Farm tourism in Spain: a gender perspective显示文摘 | Caballe A | 1999 | Geojour- nal1999，48，3： | 1 |
| 9 | Burst-fractures and Cementoplasty显示文摘 | H uet H Cabal P Gadan R | 2005 | J Neuroradiol2005，32，1： | 1 |
| 10 | Adaptive digital MPPT control for photovohaic applications 显示文摘 | Cabal C Alonso C Cid-Pastor A | 2007 | IEEE International Symposium on Industrial Electronics2007，，： | 1 |
| 11 | Monte Carlo based geometrical model for efficiency calculation of an n-type HPGe detector显示文摘 | Fatima Padilla Cabal | 2010 | Applied Radiation and Isotopes2010，68，： | 1 |
| 12 | Prevalence and mechanisms of hyperhomocysteinemia in chronic alcoholics显示文摘 | Blasco C Caballería J Deulofeu R | 2005 | Alcohol Clin Exp Res2005，29，6： | 1 |
| 13 | Avascular necrosis of the femoral head after femoral neck 显示文摘 | Bachiller FG Caballer AP Portal L F | 2002 | Clin Orthop2002，39，9： | 1 |
| 14 | Avascular necrosis of the femoral head after femoral neck fracture显示文摘 | Bachiller FG Caballer AP Portal LF | 2002 | Clin Orthop Relat Res2002，6，399： | 1 |
| 15 | Effects of S-adenosylmethionine on lipid peroxidation and liver fibrogenesis in carbon tetrachloride-induced cirrhosis显示文摘 | Marta Gassó Mireia Rubio Gregorio Varela María Cabré Joan Caballería Elena Alonso Ramón Deulofem Jordi Camps América Giménez María Pajares Albert Parés José M. Mato Joan Rodés | 1996 | Journal of Hepatology1996，，2： | 1 |
| 16 | Hyperhomocysteinemia in Liver Cirrhosis: Mechanisms and Role in Vascular and Hepatic Fibrosis显示文摘 | Elena Ruiz García-Tevijano Carmen Berasain José Antonio Rodríguez Fernando José Corrales Roberto Arias Antonio Martín-Duce Juan Caballería José María Mato Matías Antonio Avila | 2001 | Hypertension: Journal of the American Heart Association2001，，5： | 1 |
| 17 | Avascular necrosis of the femoral head after femoral neck fracture显示文摘 | Caballer AP Portal LF | 2002 | Clin Orthop2002，，399： | 1 |
| 18 | Plasma insulin level and incidence of hypertension in African and Wites 显示文摘 | Klag M J Caballer B Appel I J | 1999 | Arch Intern Med1999，159，5： | 1 |
| 19 | Hepatitis C,porphyria cutanea tarda and liver iron:an update显示文摘 | Ryan Caballes F Sendi H Bonkovsky HL | 2012 | Liver Int2012，32，6： | 1 |
| 20 | Avaseular necrosis of the fem- oral head after femoral neck fracture显示文摘 | Bachiller F Caballer AP Portal LF | 2002 | Clin Orthop Relat Res2002，399，： | 1 |
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