|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Biogenesis of Plant Prevacuolar Multivesicular Bodies显示文摘植物 prevacuolar 分隔空间(PVC ) ,或 multivesicular 身体(MVB ) ,是在调停起重要作用的单个膜界限细胞器蛋白质 trafficking 到在能分泌的小径的液泡。PVC/MVB 也在植物在 endocytic 小径用作迟了的内涵体。因为植物 PVC 超过 10 年以前作为 MVB 被识别,大进步在植物向 PVC/MVB 功能和生物的续生说的理解被取得了。在这评论,我们首先总结以前的研究进植物 PVCs/MVBs 的鉴定和描述,然后在位于 intraluminal 泡形成和植物 PVCs/MVBs 的成熟下面的机制上加亮最近的进展。另外,我们讨论看起来在植物在 autophagy 期间发生在 PVCs/MVBs 和 autophagosomes 之间的可能的串音。最后,我们在这块地里列出一些待研究的问题和现在的未来观点。 | Yong Cui Jinbo Shen Caiji Gao Xiaohong Zhuang Junqi Wang Liwen Jiang | 2016 | Molecular Plant2016，9，6： | 7 |
| 2 | AGC1.5 Kinase Phosphorylates RopGEFs to Control Pollen Tube Growth显示文摘在被子植物的两倍授精通过花粉试管要求无法移动的精子的指向的交货到卵。成长尖端的花粉试管的极性与顶端的血浆膜通过植物(ROP-GTP ) 的活跃 Rho GTPases 的动态协会被维持。为 ROP (RopGEFs ) 的 Guanine 核苷酸交换因素催化 ROP 的激活并且从而影响空间与时间的 ROP 发信号。而 RopGEFs 被发现了是 phosphorylated 蛋白质,为他们的 phosphorylation 在负责的 kinases ? vivo 和在花粉试管生长的 RopGEF phosphorylation 的生物后果仍然保持不清楚。我们这里报导细胞质的 kinases 的 Arabidopsis AGC1.5 亚科在花粉试管生长期间为 ROP-GTP 的限制本地化是批评的。AGC1.5 和 AGC1.7 功能的损失在花粉试管发信号的 ROP 下游地导致了活跃 ROP 和有缺点的事件的错误目标。AGC1.5 在容易的领域的保存重量的单位残余经由他们的催化容易的领域和 phosphorylates RopGEFs 与 RopGEFs 交往。AGC1.5 和 AGC1.7 功能的损失在花粉试管导致了 RopGEFs 的错误目标,类似于由 AGC1.5 显示 RopGEFs non-phosphorylatable 的变化引起的显型。一起,我们的结果在花粉试管的极的生长期间提供机械学的卓见进 ROP 的空间与时间的激活。 | En Li Yong Cui Fu-Rong Ge Sen Chai Wei-Tong Zhang Qiang-Nan Feng Liwen Jiang Sha Li Yan Zhang | 2018 | Molecular Plant2018，11，9： | 5 |
| 3 | Fast-Suppressor Screening for New Components in Protein Trafficking,Organelle Biogenesis and Silencing Pathway in Arabidopsis thaliana Using DEX-Inducible FREE1-RNAi Plants显示文摘Membrane trafficking is essential for plant growth and responses to external signals.The plant unique FYVE domain-containing protein FREE1 is a component of the ESCRT complex(endosomal sorting complex required for transport).FREE1 plays multiple roles in regulating protein trafficking and organelle biogenesis including the formation of intraluminal vesicles of multivesicular body(MVB),vacuolar protein transport and vacuole biogenesis,and autophagic degradation.FREE1 knockout plants show defective MVB formation,abnormal vacuolar transport,fragmented vacuoles,accumulated autophagosomes,and seedling lethality.To further uncover the underlying mechanisms of FREE1 function in plants,we performed a forward genetic screen for mutants that suppressed the seedling lethal phenotype of FREE1-RNAi transgenic plants.The obtained mutants are termed as suppressors of free1(sof).To date,229 putative sof mutants have been identified.Barely detecting of FREE1 protein with M3plants further identified 84 FREE1-related suppressors.Also145 mutants showing no reduction of FREE1 protein were termed as RNAi-related mutants.Through next-generation sequencing(NGS)of bulked DNA from F2mapping population of two RNAi-related sof mutants,FREE1-RNAi T-DNA inserted on chromosome 1 was identified and the causal mutation of putative sof mutant is being identified similarly.These FREE1-and RNAi-related sof mutants will be useful tools and resources for illustrating the underlying mechanisms of FREE1 function in intracellular trafficking and organelle biogenesis,as well as for uncovering the new components involved in the regulation of silencing pathways in plants. | Qiong Zhao Caiji Gao PoShing Lee Lin Liu Shaofang Li Tangjin Hu Jinbo Shen Shuying Pan Hao Ye Yunru Chen Wenhan Cao Yong Cui Peng Zeng Sheng Yu Yangbin Gao Liang Chen Beixin Mo Xin Liu Shi Xiao Yunde Zhao Silin Zhong Xuemei Chen Liwen Jiang | 2015 | Journal of Genetics and Genomics2015，42，6： | 4 |
| 4 | Differentiation of neuron-like cells from mouse parthenogenetic embryonic stem cells显示文摘Parthenogenetic embryonic stem cells have pluripotent differentiation potentials,akin to fertilized embryo-derived embryonic stem cells.The aim of this study was to compare the neuronal differentiation potential of parthenogenetic and fertilized embryo-derived embryonic stem cells.Before differentiation,karyotype analysis was performed,with normal karyotypes detected in both parthenogenetic and fertilized embryo-derived embryonic stem cells.Sex chromosomes were identified as XX.Immunocytochemistry and quantitative real-time PCR detected high expression of the pluripotent gene,Oct4,at both the mRNA and protein levels,indicating pluripotent differentiation potential of the two embryonic stem cell subtypes.Embryonic stem cells were induced with retinoic acid to form embryoid bodies,and then dispersed into single cells.Single cells were differentiated in N2 differentiation medium for 9 days.Immunocytochemistry showed parthenogenetic and fertilized embryo-derived embryonic stem cells both express the neuronal cell markers nestin,βIII-tubulin and myelin basic protein.Quantitative real-time PCR found expression of neurogenesis related genes(Sox-1,Nestin,GABA,Pax6,Zic5 and Pitx1) in both types of embryonic stem cells,and Oct4 expression was significantly decreased.Nestin and Pax6 expression in parthenogenetic embryonic stem cells was significantly higher than that in fertilized embryo-derived embryonic stem cells.Thus,our experimental findings indicate that parthenogenetic embryonic stem cells have stronger neuronal differentiation potential than fertilized embryo-derived embryonic stem cells. | Xingrong Yan Yanhong Yang Wei Liu Wenxin Geng Huichong Du Jihong Cui Xin Xie Jinlian Hua Shumin Yu Liwen Li Fulin Chen | 2013 | Neural Regeneration Research2013，8，4： | 4 |
| 5 | Atacamite and Nantokite in Kaerqueka Copper Deposit of Qimantag Area:Evidence for Cenozoic Climate Evolution of the Qaidam Basin显示文摘Using a mineralogy method to reflect climate evolution is a new approach to research Cenozoic environmental progression of the Qaidam Basin. In this paper, we present the chemical composition and crystallographic parameter of atacamite, nantokite, and tenorite in cryptocrystalline aggregates from the Kaerqueka copper deposit of Qimantag metallogenic belt by means of electron microprobe and in-situ X-ray diffraction analyses. Atacamite and nantokite occur in an intimate intergrowth as the secondary precipitation of chalcopyrite and bornite filling in the interstitial space of andradite in the Kaerqueka copper deposit, with an average composition of Cl: 12.38 wt.%, Cu: 63.76 wt.%, O: 21.46 wt.%. X-ray microdiffraction shows that the intergrowth contains nantokite with a cubic unit cell a=5.403(2) ? and atacamite with an orthorhombic unit cell a=6.030(3), b=6.883(2), c=9.114(1) ?. XRD quantitative calculation shows that the nanometric aggregate contains 36.07 wt.% tenorite, 18.41 wt.% atacamite, and 45.52 wt.% nantokite. The presence of nantokite and atacamite requires saline solutions for their formation and hyper-arid climate conditions for their preservation. Combined with the data of salt lakes and the pollen sequence of western China, we suggest that during the uplift of the Tibetan Plateau and the retreat of the Paratethys, saline water was forced to the surface through a basal fracture zone. In the hyper-arid climate of the Qaidam Basin, the recharge of groundwater by direct precipitation is negligible, and groundwater is derived from inflow from the salt lakes. Thus, atacamite is preserved. In addition, spertiniite in the edge and fractures of atacamite and nantokite may represent wetter climate after the formation of atacamite and nantokite. | Liwen Yi Xiangping Gu Anhuai Lu Xiangyu Li Dexian Zhang Zhiling Wang Jianping Liu Shuai Li Zhengxiang Shu Cui Yu Hongyan Zuo Can Shen | 2017 | Journal of Earth Science2017，28，3： | 3 |
| 6 | Major and Trace Elements of Magnetite from the Qimantag Metallogenic Belt: Insights into Evolution of Ore–forming Fluids显示文摘Magnetite, as a genetic indicator of ores, has been studied in various deposits in the world. In this paper, we present textural and compositional data of magnetite from the Qimantag metallogenic belt of the Kunlun Orogenic Belt in China, to provide a better understanding of the formation mechanism and genesis of the metallogenic belt and to shed light on analytical protocols for the in situ chemical analysis of magnetite. Magnetite samples from various occurrences, including the ore–related granitoid pluton, mineralised endoskarn and vein–type iron ores hosted in marine carbonate intruded by the pluton, were examined using scanning electron microscopy and analysed for major and trace elements using electron microprobe and laser ablation–inductively coupled plasma–mass spectrometry. The field and microscope observation reveals that early–stage magnetite from the Hutouya and Kendekeke deposits occurs as massive or banded assemblages, whereas late–stage magnetite is disseminated or scattered in the ores. Early–stage magnetite contains high contents of Ti, V, Ga, Al and low in Mg and Mn. In contrast, late–stage magnetite is high in Mg, Mn and low in Ti, V, Ga, Al. Most magnetite grains from the Qimantag metallogenic belt deposits except the Kendekeke deposit plot in the ' Skarn ' field in the Ca+Al+Mn vs Ti+V diagram, far from typical magmatic Fe deposits such as the Damiao and Panzhihua deposits. According to the(Mg O+Mn O)–Ti O2–Al2O3 diagram, magnetite grains from the Kaerqueka and Galingge deposits and the No.7 ore body of the Hutouya deposit show typical characteristics of skarn magnetite, whereas magnetite grains from the Kendekeke deposit and the No.2 ore body of the Hutouya deposit show continuous elemental variation from magmatic type to skarn type. This compositional contrast indicates that chemical composition of magnetite is largely controlled by the compositions of magmatic fluids and host rocks of the ores that have reacted with the fluids. Moreover, a combination of petrography and magnetite geochemistry indicates that the formation of those ore deposits in the Qimantag metallogenic belt involved a magmatic–hydrothermal process. | YI Liwen GU Xiangping LU Anhuai LIU Jianping LEI Hao WANG Zhiling CUI Yu ZUO Hongyan SHEN Can | 2015 | Acta Geologica Sinica(English Edition)2015，89，4： | 2 |
| 7 | A Ce-Cu-Ti oxidecatalyst for the selective catalytic reduction of NO with NH3显示文摘 | Gao Xiang Du Xuesen Cui Liwen | 2010 | Catalysis Communications2010，12，： | 1 |
| 8 | The roles of endomembrane trafficking in plant abiotic stress responses显示文摘Endomembrane trafficking is a fundamental cellular process in all eukaryotic cells and its regulatory mechanisms have been extensively studied.In plants,the endomembrane trafficking system needs to be constantly adjusted to adapt to the ever-changing environment.Evidence has accumulated supporting the idea that endomembrane trafficking is tightly linked to stress signaling pathways to meet the demands of rapid changes in cellular processes and to ensure the correct delivery of stress-related cargo molecules.However,the underlying mechanisms remain unknown.In this review,we summarize the recent findings on the functional roles of both secretory trafficking and endocytic trafficking in different types of abiotic stresses.We also highlight and discuss the unique properties of specific regulatory molecules beyond their conventional functions in endosomal trafficking during plant growth under stress conditions. | Xiangfeng Wang Min Xu Caiji Gao Yonglun Zeng Yong Cui Wenjin Shen Liwen Jiang | 2020 | Journal of Integrative Plant Biology2020，62，1： | 1 |
| 9 | Investigation of the effect of Cu addition on the SO2-resistance of a Ce-Ti oxide catalyst for selective catalytic reduction of NO with NH3 显示文摘 | Du Xuesen Gao Xiang Cui Liwen | 2012 | Fuel2012，92，： | 1 |
| 10 | Analysis on Green Development Strategy of Xinjiang Cotton显示文摘Through on-the-spot investigation and survey in Xinjiang cotton region and relevant data( containing statistical data,online information and government documents,etc.),the characteristics of water resource,wind-sand disaster,soil salinization,cotton diseases,insect pests and weeds in Xinjiang are studied. It is proposed reasons and specific programmes of green development strategy of Xinjiang cotton,and specific strategies contain ecological water supply,land desertification control,soil improvement,and ecological control of diseases,insect pests and weeds,thereby providing the support for sustainable development of Xinjiang cotton. | Tian Liwen Tian Conghua Cui Jianping Lin Tao | 2019 | Meteorological and Environmental Research2019，10，1： | 1 |
| 11 | Single nucleotide polymorphisms in the D-loop region of mitochondrial DNA and age-at-onset of patients with chronic kidney disease显示文摘 | Bai Yaling Guo Zhanjun Xu Jinsheng Zhang Junxia Cui Liwen Zhang Huiran Zhang Shenglei Ai Xiaolu | 2014 | Chinese Medical Journal2014，，17： | 1 |
| 12 | The research on cable force measurement based on degenerated element with frequency method显示文摘 | CUI Xin JIANG Yong FU Liwen | 2013 | Applied Mechanics and Mate- rials2013，，405408： | 1 |
| 13 | A Ce-Cu-Ti oxide catalyst for the selective catalytic reduction of NO with NH3 显示文摘 | Gao Xiang Du Xuesen Cui Liwen | 2010 | Catalysis Communications2010，12，4： | 1 |
| 14 | Exploring the salt-and drought-tolerant genes of alfalfa through expression library screening strategy显示文摘Salinity and drought stress are major abiotic stresses negatively affecting the growth and productivity of alfalfa(Medicago sativa L.).Exploration of genes exhibiting superior tolerance to salinity and drought stress in alfalfa will help aid target molecular breeding and developing tolerant forages.In this study,we adopted a high-efficient yeast FOX hunting system for the identification of salinity and drought tolerant genes in alfalfa.Based on the Gateway-compatible vector system,a high-quality expression library was constructed,containing 1.3×10^(7) clones with an average size of 1.44 kb.Through heterologous transformation of mixed library plasmid into salt or drought sensitive yeast mutants,monoclonal resistant strains were screened and tolerant genes were captured.Eighteen salinity-tolerance genes were obtained which were involved in several pathways,containing GRAS and zinc finger transcriptional factors,PP2A interaction module,ERVT vesicle transporter and LETM transmembrane protein.Twelve drought tolerance genes were separated,including ERF and SCL transcriptional factors,CIPK and BSK protein kinases,TGL-type ligase,cPGM and cPDL protease.The mRNA transcription levels of these tolerant genes were inducible or suppressible for response to salt or drought stress conditions following quantitative PCR detection,respectively.Furthermore,heterologous transformation of ERVT and CIPK11 can improve the salt and drought stress tolerance in Arabidopsis,which indicates the conservative function of the screening gene in yeast and Arabidopsis.Obtaining these candidate genes can provide new insights for future research with respect to plant salt and drought tolerance. | Zhihua Li Yue Zou Yanpeng Li Cancan Sun Yu Liu Liwen Cui Jun Liu Zhimin Yang Yu Chen | 2021 | Grass Research2021，1，1： | 0 |
| 15 | Over-expression of Gene FaASR Promotes Strawberry Fruit Coloring显示文摘Fruit development and ripening is a complicate process. Although much progress has been made on the ripenig process, the molecular mechamism of fruit development is not yet clear. In this study, we used ‘Sweet Charlie' strawberry as test materials, based on cloning the strawberries ASR homologous gene, we carried out the bioinformatics and temporal expression analysis of Fa ASR, by manipulating ASR gene expression level in strawberry fruit, we tested the changes of physiological indicators, including sugar, ABA, pigments content, and fruit firmness, as well as phenotypic changes. In addition, we measured the expression changes of some anthocyanin-related gene, such as CHS and UFGT, by which we revealed the regulation mechanisms of ASR gene over strawberry fruit ripening. Strawberry ASR contained a typical domain of ABA/WDS that was related to fruit ripening and stress-resistance, and ASR gene over-expression could promote strawberry fruit coloring, endogenous ABA and sucrose accumulation, fruit softening, and induced the transcription levels of anthocyanin-related genes CHS and UFGT. The present study will further reveal the molecular mechanisms of information transmission in fruit development, and will also play an important foundation for future molecular improvement of strawberries breeding. | LIU Zhongjie ZHAO Pengcheng CUI Liwen FANG Jinggui JIA Haifeng | 2015 | Horticultural Plant Journal2015，1，3： | 0 |
| 16 | Mining candidate genes of grape berry cracking based on high density genetic map显示文摘Fruit cracking is a phenomenon in which the peel cracks during grape berry development,which seriously affects the yield and quality of the fruit.However,there are few studies on the mining of candidate genes related to berry cracking.In order to better understand the genetic basis of berry cracking,we used the results of previous quantitative trait locus(QTL)mapping,combined with field surveys of berry-cracking types and the berry-cracking rate,to mine candidate berry-cracking genes.The results showed that three identical QTL loci were detected in two years(2019 and 2020);and three candidate genes were annotated in the QTL interval.In mature berries,the expressions of the candidate genes were more abundant in the cracking-susceptible parent(‘Crimson Seedless’)than in the cracking-resistant parent(‘Muscat Hamburg’).Grape berry cracking is a complex trait controlled by multiple genes,mainly including genes encoding cellulose synthase–like protein H1,glucan endo-1,3-beta-glucosidase 12,and brassinosteroid insensitive 1-associated receptor kinase 1.The high expression of the candidate berry-cracking genes may promote the occurrence of berry cracking.This study helps elucidate the genetic mechanism of grape berry cracking. | Chuan Zhang Liwen Cui Chonghuai Liu Xiucai Fan Jinggui Fang | 2023 | Horticultural Plant Journal2023，9，4： | 0 |
| 17 | Novel biomarkers identifying hypertrophic cardiomyopathy and its obstructive variant based on targeted amino acid metabolomics显示文摘Background:Hypertrophic cardiomyopathy(HCM)is an underdiagnosed genetic heart disease worldwide.The management and prognosis of obstructive HCM(HOCM)and non-obstructive HCM(HNCM)are quite different,but it also remains challenging to discriminate these two subtypes.HCM is characterized by dysmetabolism,and myocardial amino acid(AA)metabolism is robustly changed.The present study aimed to delineate plasma AA and derivatives profiles,and identify potential biomarkers for HCM.Methods:Plasma samples from 166 participants,including 57 cases of HOCM,52 cases of HNCM,and 57 normal controls(NCs),who first visited the International Cooperation Center for HCM,Xijing Hospital between December 2019 and September 2020,were collected and analyzed by high-performance liquid chromatography-mass spectrometry based on targeted AA metabolomics.Three separate classification algorithms,including random forest,support vector machine,and logistic regression,were applied for the identification of specific AA and derivatives compositions for HCM and the development of screening models to discriminate HCM from NC as well as HOCM from HNCM.Results:The univariate analysis showed that the serine,glycine,proline,citrulline,glutamine,cystine,creatinine,cysteine,choline,and aminoadipic acid levels in the HCM group were significantly different from those in the NC group.Four AAs and derivatives(Panel A;proline,glycine,cysteine,and choline)were screened out by multiple feature selection algorithms for discriminating HCM patients from NCs.The receiver operating characteristic(ROC)analysis in Panel A yielded an area under the ROC curve(AUC)of 0.83(0.75-0.91)in the training set and 0.79(0.65-0.94)in the validation set.Moreover,among 10 AAs and derivatives(arginine,phenylalanine,tyrosine,proline,alanine,asparagine,creatine,tryptophan,ornithine,and choline)with statistical significance between HOCM and HNCM,3 AAs(Panel B;arginine,proline,and ornithine)were selected to differentiate the two subgroups.The AUC values in the training and validation sets for Panel B were 0.83(0.74-0.93)and 0.82(0.66-0.98),respectively.Conclusions:The plasma AA and derivatives profiles were distinct between the HCM and NC groups.Based on the differential profiles,the two established screening models have potential value in assisting HCM screening and identifying whether it is obstructive. | Lanyan Guo Bo Wang Fuyang Zhang Chao Gao Guangyu Hu Mengyao Zhou Rutao Wang Hang Zhao Wenjun Yan Ling Zhang Zhiling Ma Weiping Yang Xiong Guo Chong Huang Zhe Cui Fangfang Sun Dandan Song Liwen Liu Ling Tao | 2022 | Chinese Medical Journal2022，135，16： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员