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| 1 | Osteogenic growth peptide enhances the proliferation of bone marrow mesenchymal stem cells from osteoprotegerin-deficient mice by CDK2/cyclin A显示文摘支持骨头形成是在骨质疏松症治疗的基本策略之一,破裂修理。作为骨头形成上的激发器之一, osteogenic 生长肽(OGP ) 在 vitro 并且在 vivo, osteoprotegerin (OPG ) 在被建议了被包含增加造骨细胞的增长和区别。在这研究,我们在骨头髓上评估了 OGP 的效果从在由 3-(4,5-dimethylthiazol-2-yl ) 的 vitro 的 OPG 缺乏的老鼠的间充质的干细胞(MSC )-2,5-diphenyltetrazolium 溴化物试金,碱的磷酸酶(高山) 活动试金,即时聚合酶链反应,和西方的污点分析。结果证明 OGP 刺激了 MSC 增长并且在 mRNA 和蛋白质层次两个都在 MSC 增加了 CDK2 和 cyclin A 的表示。然而, OGP 的 differentiative 效果都没作为高山活动和 Runx2 的表示层次被显示出, Osterix 没被 OGP 显著地增加。我们的学习建议 OGP 可以由刺激 MSC 增长而非区别在 OPG 缺乏的老鼠增加骨头形成,并且可能由触发 CDK2/cyclin 一条小径。 | Qinming Fei Changjun Guo Xiaoya Xu Jianjun Gao Jian Zhang Tongyi Chen Dafu Cui | 2010 | Acta Biochimica et Biophysica Sinica2010，42，11： | 11 |
| 2 | Hyaluronic acid facilitates bone repair effects of calcium phosphate cement by accelerating osteogenic expression显示文摘Calcium phosphate cements(CPC)are widely anticipated to be an optimum bone repair substitute due to its satisfied biocompatibility and degradability,suitable to be used in minimally invasive treatment of bone defects.However the clinical application of CPC is still not satisfied by its poor cohesiveness and mechanical properties,in particular its osteoinductivity.Hyaluronic acid reinforced calcium phosphate cements(HA/CPC)showed extroadinary potential not only enhancing the compressive strength of the cements but also significantly increasing its osteoinductivity.In our study,the compressive strength of HA/CPC increased significantly when the cement was added 1%hyaluronic acid(denoted as 1-HA/CPC).In the meantime,hyaluronic acid obviously promoted ALP activity,osteogenic related protein and mRNA expression of hBMSCs(human bone marrow mesenchymal stem cells)in vitro,cement group of HA/CPC with 4%hyaluronic acid adding(denoted as 4-HA/CPC)showed optimal enhancement in hBMSCs differentiation.After being implanted in rat tibial defects,4-HA/CPC group exhibited better bone repair ability and bone growth promoting factors,comparing to pure CPC and 1-HA/CPC groups.The underlying biological mechanism of this stimulation for HA/CPC may be on account of higher osteogenic promoting factors secretion and osteogenic genes expression with hyaluronic acid incorporation.These results indicate that hyaluronic acid is a highly anticipated additive to improve physicochemical properties and osteoinductivity performance of CPCs for minimally invasive healing of bone defects. | Xu Cui Chengcheng Huang Zhizhen Chen Meng Zhang Chunyu Liu Kun Su Jianyun Wang Li Li Renxian Wang Bing Li Dafu Chen Changshun Ruan Deping Wang William W.Lu Haobo Pan | 2021 | Bioactive Materials2021，6，11： | 6 |
| 3 | Design and optimization of DBR in 980 nm bottom-emitting VCSEL显示文摘According to the theory of DBR,with the P-type DBR as an example,the electrical characteristics and optical reflection of the DBR are analyzed by studying the energy band structure with various graded region widths and doping densities. The width and doping density of graded region are decided through a comparative study. The P-type DBR of 980 nm VCSELs is designed with Al0.9Ga0.1As and Al0.1Ga0.9As selected as the high and low refractive index material for the DBR. The 980 nm bottom VCSELs,which consists of 30 pairs P-type DBR and 28 pairs N-type DBR,are then fabricated. In P-type DBR,the width of graded region is 0.02 μm and the uniformity doping concentration is 2.5×1018cm-3. Its reflectivity is 99.9%. In N-type DBR,the width of graded region is also 0.02 μm and the uniformity doping concen-tration is 2×1018cm-3. Its reflectivity is 99.3%. The I-V curve shows that the series resistance of the device is about 0.05 Ω. According to the theory of DBR,with the P-type DBR as an example,the electri-cal characteristics and optical reflection of the DBR are analyzed by studying the energy band structure with various graded region widths and doping densities. The width and doping density of graded re-gion are decided through a comparative study. The P-type DBR of 980 nm VCSELs is designed,with Al0.9Ga0.1As and Al0.1Ga0.9As selected as the high and low refractive index material for the DBR. The 980 nm bottom VCSELs,which consist of 30 pairs P-type DBR and 28 pairs N-type DBR,are then fabri-cated. In P-type DBR,the width of graded region is 0.02 μm and the uniformity doping concentration is 2.5×1018cm-3. Its reflectivity is 99.9%. In N-type DBR,the width of graded region is also 0.02 μm and the uniformity doping concentration is 2×1018cm-3. Its reflectivity is 99.3%. The I-V curve shows that the series resistance of the device is about 0.05 Ω. | LI Te NING YongQiang HAO ErJuan CUI JinJiang ZHANG Yan LIU GuangYu QIN Li LIU Yun WANG LiJun CUI DaFu XU ZuYan | 2009 | Science in China(Series F)2009，52，7： | 4 |
| 4 | Strontium modulates osteogenic activity of bone cement composed of bioactive borosilicate glass particles by activating Wnt/β-catenin signaling pathway显示文摘There is a need for synthetic grafts to reconstruct large bone defects using minimal invasive surgery.Our previous study showed that incorporation of Sr into bioactive borate glass cement enhanced the osteogenic capacity in vivo.However,the amount of Sr in the cement to provide an optimal combination of physicochemical properties and capacity to stimulate bone regeneration and the underlying molecular mechanism of this stimulation is yet to be determined.In this study,bone cements composed of bioactive borosilicate glass particles substituted with varying amounts of Sr(0 mol%to 12 mol%SrO)were created and evaluated in vitro and in vivo.The setting time of the cement increased with Sr substitution of the glass.Upon immersion in PBS,the cement degraded and converted more slowly to HA(hydroxyapatite)with increasing Sr substitution.The released Sr2+modulated the proliferation,differentiation,and mineralization of hBMSCs(human bone marrow mesenchymal stem cells)in vitro.Osteogenic characteristics were optimally enhanced with cement(designated BG6Sr)composed of particles substituted with 6mol%SrO.When implanted in rabbit femoral condyle defects,BG6Sr cement supported better peri-implant bone formation and bone-implant contact,comparing to cements substituted with 0mol%or 9mol%SrO.The underlying mechanism is involved in the activation of Wnt/β-catenin signaling pathway in osteogenic differentiation of hBMSCs.These results indicate that BG6Sr cement has a promising combination of physicochemical properties and biological performance for minimally invasive healing of bone defects. | Xu Cui Yadong Zhang Jianyun Wang Chengcheng Huang Yudong Wang Hongsheng Yang Wenlong Liu Ting Wang Deping Wang Guocheng Wang Changshun Ruan Dafu Chen William W.Lu Wenhai Huang Mohamed N.Rahaman Haobo Pan | 2020 | Bioactive Materials2020，5，2： | 3 |
| 5 | Beneficial effects of C-peptide on renal morphology in diabetic rats显示文摘学习被承担在 glomerular 上检验 C 肽的效果在有差或中等的 glycemic 的糖尿病的老鼠控制的 streptozotocin (STZ ) 的体积(VGLOM ) , mesangial 矩阵合成,和降级。(差的 glycemic 控制) 系列 1 包括了一些健康老鼠, hyperglycemic 老鼠,糖尿病的对待胰岛素的老鼠和糖尿病的 C-peptide-treated 老鼠。(中等 glycemic 控制) 系列 2 包括了一些健康老鼠,糖尿病的对待胰岛素的老鼠,糖尿病的 insulin-peptide-treated 和 C-peptide-treated 老鼠。在 8 个星期以后,左肾经由轻显微镜学为 VGLOM 和 mesangial 矩阵区域的评估被切除。Mesangial 房间是有教养的因为 48 h 和类型 IV 骨胶原表示和矩阵 metalloproteinase (MMP )-2 表示被 ELISA 和 RTPCR 测量。结果显示在系列 1, C 肽管理在 VGLOM 和 mesangial 矩阵区域压制了导致糖尿病的增加。在系列 2, C 肽管理在 mesangial 矩阵区域在 VGLOM 和更大的减少导致了类似的减少什么时候与胰岛素治疗相比独自一个。而且, C 肽(300 nM ) 完全在在 30 公里葡萄糖媒介有教养的 mesangial 房间禁止了骨胶原 IV mRNA 表示和蛋白质集中的导致葡萄糖的增加。MMP-2 mRNA 表示没被 C 肽影响。在结论,到为 8 个星期的 STZ 糖尿病的老鼠的 C 肽管理导致 mesangial 矩阵的导致糖尿病的扩大的抑制。这效果独立于 glycemic 控制的水平,从 mesangial 的导致糖尿病的过多的形成的抑制的结果打 IV 骨胶原。 | Wei Sun Xin Gao Xiaolong Zhao Dafu Cui Qichang Xia | 2010 | Acta Biochimica et Biophysica Sinica2010，42，12： | 3 |
| 6 | STUDY OF CAPILLARY ELECTROPHORESIS ON MICROCHIP BASED ON MEMS显示文摘Using a standard photolithographical procedure, chemical wet etching and thermal diffusion bonding technology, a chemical analysis device for Capillary Electrophoresis(CE) has been microfabricated on a planar glass substrate with a cross-column geometry. The channels on the microchip substrate are about 50μm deep and 150μm wide. By employing amino acids derived from 2,4-DiNitroFluoroBenzen (DNFB) on CE chip channels, the sample manipulating system is studied based on the principle of electrodynamics. | Wang Ming Li Wei Han Jinghong Cui Dafu (State Key Lab. of Transducer Tech., Inst. of Electron., Chinese Academy of Sci., Beijing 100080) | 2002 | Journal of Electronics(China)2002，19，4： | 1 |
| 7 | Fabrication and Charac- terization of A Double-Heater Based MEMS Thermal Flow Sensor 显示文摘 | Sun Jianhai Cui Dafu Zhang Lulu | 2013 | Sensors and Actuators A2013，193，： | 1 |
| 8 | Phase transition behavior of BaTiO3 thin films using high-temperature X-ray diffraction显示文摘 | Li Chunling Chen Zhenghao Cui Dafu | 1999 | Journal of Applied Physics1999，86，： | 1 |
| 9 | Large X^(3) determined by the degenerate four- wave mixing in a GaAs/AlGaAs step multiple quantum well显示文摘 | MA Jianwei CHEN Zhenghao CUI Dafu | 1996 | Opt Commun1996，124，： | 1 |
| 10 | Development of Surface Plasmon Resonance-Based Biosensor Immunoassay for Sulfamethoxazole显示文摘The illegal use of veterinary drugs has led to the occurrence of residues in food of animal origin.This is a hazard to people's health.In this paper,a sensitive and fast assay was developed to determine sulfamethoxazole (SMOZ) in PBS with a homemade surface plasmon resonance (SPR)-based biosensor which had a single flow channel and two references.The derivative of SMOZ was covalently immobilized to a carboxymethyldextran modified gold film.A recently developed monoclonal antibody against sulfamethoxazole was used to develop an inhibitive immunobiosensor assay in which the antibody was mixed with SMOZ of different concentrations prepared with PBS to construct a standard curve with a low limit of detection(LOD).The effect of matrix in milk was also studied.Calculating of the apparent concentration showed the cross-reactivity with other sulfonamides. | Hui Li Dafu Cui Yujie Wang Hao-yuan Cai Junbo Wang Zipan Zheng | 2006 | 稀有金属材料与工程2006，35，A03： | 1 |
| 11 | Raman spectroscopy of laser ablated BaTiO3/ YBa2Cu3O7-x thin films bilayer structure 显示文摘 | CUI Dafu LI Chunling MA Kun | 1996 | Appl Phys Lett1996，68，6： | 1 |
| 12 | Monolithically integrated PCR biochip for DNA amplification 显示文摘 | Cui Zheng Cui Dafu | 2003 | Sens Actuat A2003，108，： | 1 |
| 13 | SIN Beam Resonant PressureSensors with a Novel Structure显示文摘 | Chen Deyong Cui Dafu Wang Li | 2002 | IEEE2002，，： | 1 |
| 14 | 4350<ce:hsp sp='0.25'/>W quasi-continuous-wave operation of a diode face-pumped ceramic Nd: YAG slab laser显示文摘 | Junchi Chen Jiang Li Jialin Xu Wenbin Liu Yong Bo Xiqi Feng Yiting Xu Dongliang Jiang Zhongzheng Chen Yubai Pan Yading Guo Biao Yan Chang Guo Lei Yuan Hongtao Yuan Yanyong Lin Yunsheng Xiao Qinjun Peng Wenqiang Lei Dafu Cui Zuyan Xu | 2014 | Optics and Laser Technology2014，，： | 1 |
| 15 | Insulin analogs with B24 or B25 phenylalanine replaced by biphenylalanine显示文摘B24 和 B25 本氨基丙酸(Phe ) 在胰岛素结构和功能起重要作用。有联本基丙氨酸(Bip ) 代替的 B24 Phe 或 B25 Phe 的胰岛素类似物被酶的半合成准备。生物活动被受体绑定试金并且在 vivo 鼠标骚动试金决定。结果证明那 B25 Bip 胰岛素举办 139% 受体绑定活动,建议而, B24 Bip 胰岛素与本国的胰岛素相比是不活跃的,那 B24 Phe 为胰岛素活动是关键的并且 50% 在 vivo 生物活动。在答案的结构被圆形的二色性和 fluoremetry 学习,并且我们的结果建议有低活动的胰岛素类似物趋于是紧包装的更多。协会性质被尺寸排除层析学习。在 deshexapeptide 胰岛素的 B24 Phe 或在 despentapeptide 胰岛素的 B25 Phe 的 Bip 酰胺代替将引起 monomeric B24 Phe 酰胺 deshexapeptide 胰岛素或 B25 Phe 酰胺 despentapeptide 胰岛素联系并且形成暗淡 ers,而在胰岛素的 B24 Phe 的变化将使胰岛素成为暗淡 ers 分裂进胰岛素单体。 | Haijuan Du Jiahao Shi Dafu Cui Youshang Zhang | 2008 | Acta Biochimica et Biophysica Sinica2008，40，2： | 1 |
| 16 | STUDY OF THE PROPERTIES OF INFRARED INTERSUBBAND TRANSITION IN DOPED GaAs/A1_(x)Ga_(1-x)As MULTIPLE QUANTUM WELLS显示文摘The properties of intersubband transition of GaAs/A1_(x)Ga_(1-x)As multiple quantum wells with various well widths and doped-well concentrations have been studied.Both theoretical and experimental results are in good agreement.For the appropriate well width and higher doping concentration,we directly observed two intersubband absorption peaks from E_(1)→E_(2) and E_(2)→E_(3) transitions in well.The experimental results and theoretical analysis are given. | CHEN Zhenghao CUI Dafu ZHOU Junming PAN Shaohua HUANG Qi ZHOU Yueliang LÜHuibin XIE Yuanlin FENG Simin YANG Guozhen | 1990 | Chinese Physics Letters1990，7，7： | 0 |
| 17 | Isolation of plasma from whole blood using a microfludic chip in a continuous cross-flow显示文摘A novel microfluidic chip is developed for crossflow filtration plasma from the whole blood which is carried out in a continuous manner. This microfluidic chip was made of a silicon substrate sealed with a compound cover. The silicon substrate fabricated by micro-electro-mechanical system (MEMS) technology consisted of microposts array, microchannels and reservoirs. Then the silicon substrate was characterized by Scaning Electron Microscopy (SEM). The performance of the microfluidic chip was valued by the experiments of plasma isolation. During more than one hour of continuous blood infusion through the chip, there were no problems of jamming or clogging, and the plasma selectivity of 97.78% was achieved. Due to the chip’s simple structure and control mechanism with a continuous, real time operating manner, this microfluidic chip is easily expected to be integrated into micro total analytical system (μTAS) which will create a microanalysis system for point-of-care diagnostics. | CHEN Xing CUI DaFu ZHANG LuLu | 2009 | Chinese Science Bulletin2009，54，2： | 0 |
| 18 | YBa_(2)Cu_(3)O_(7-x) Superconducting Thin Films Grown on CaNdA1O_(4)(100) Substrates显示文摘We report the fabrication of high temperature superconducting YBa_(2)Cu_(3)O_(7-x) thin films on CaNdA1O_(4)(100) substrate by pulsed laser deposition.Films have excellent features.X-ray diffraction patterns show that thin films are epitaxial with c-axis orientation normal to the surface of su bstrate.The four-terminal measurements indicate the zero resistance temperature of 92.1 K,and transition width of 0.7K,the critical current density of 3×10^(6)A/cm^(2)at 77K and under zero magnetic field. | XU Shifa ZHOU Yueliang LÜHuibin CUI Dafu CHEN Zhenghao YANG Guozhen TIAN Yongjun LIU Junzheng LI Lin CHANG Yingchuan HOU Desen FAN Hui ZHANG Zuren | 1994 | Chinese Physics Letters1994，11，5： | 0 |
| 19 | Biosensors and Biochips Based on BioMEMS and Nanotechnology显示文摘In the past two decades,the biological and medical fields have seen great advances in the development of biosensors and bioehips capable of characterizing and quantifying biomolecules.This lecture is meant to discuss the development and applications of advanced electroanalysis,biophotonics,nanotechnology,MEMS- based biosensors and biochips for biomedical diagnostics and physical performances of athlete. | Xinxia Cai Dafu Cui | 2006 | 稀有金属材料与工程2006，35，A03： | 0 |
| 20 | Transverse Electric and Transverse Magnetic Active Intersubband Transitions in CaAs/AlGaAs Step Quantum Well显示文摘We have observed transverse electric(TE)and transverse magnetic(TM)intersubband infrared absorption peaks in GaAs/AlGaAs step multiquantum wells(SMQW).The energy splitting between TE and TM is 13meV.The high conversion efficiency of second harmonic signal due to intersubband transition in the GaAs/AlGaAs SMQW with a waveguiding surface-emitting structure have been obtained.The second harmonic generation(SffG)obeys wave vector conservation and propagates perpendicular to the waveguide surface which shows that the nonlinear polarization field for SHG may be TE as well as TM active.The existence of strong surface-emitting second harmonic signal also offers solid evidence of TE polarization active intersubband transition in GaAs/AlGaAs SMQW structure. | CUI Dafu CHEN Zhenghao YUAN Zhenyu ZHOU Yueliang LÜ Huibin YANG Guozhen | 1994 | Chinese Physics Letters1994，11，12： | 0 |
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