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| 1 | Isolation and characterisation of human gingival margin-derived STRO-1/MACS^+ and MACS^-cell populations显示文摘Recently,gingival margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting(MACS) showed remarkable periodontal regenerative potential in vivo.As a second-stage investigation,the present study's aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive(MACS^+) and STRO-1-negative(MACS^-) cell populations from the human free gingival margin.Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies.Subsequently,the MACS^+ and MACS^- cell fractions were characterized by flow cytometry for expression of CD14,CD34,CD45,CD73,CD90,CD105,CD146/MUC18 and STRO-1.Colony-forming unit(CFU) and multilineage differentiation potential were assayed for both cell fractions.Mineralisation marker expression was examined using real-time polymerase chain reaction(PCR).MACS^+ and MACS- cell fractions showed plastic adherence.MACS^+ cells,in contrast to MACS- cells,showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs(P<0.01).More than 95%of MACS^+ cells expressed CD105,CD90 and CD73;lacked the haematopoietic markers CD45,CD34 and CD14,and expressed STRO-1 and CD146/MUC18.MACS- cells showed a different surface marker expression profile,with almost no expression of CD14 or STRO-1,and more than 95%of these cells expressed CD73,CD90 and CD146/MUC18,as well as the haematopoietic markers CD34 and CD45 and CD105.MACS^+ cells could be differentiated along osteoblastic,adipocytic and chondroblastic lineages.In contrast,MACS- cells demonstrated slight osteogenic potential.Unstimulated MACS^+ cells showed significantly higher expression of collagen I(P<0.05) and collagen III(P<0.01),whereas MACS^- cells demonstrated higher expression of osteonectin(P<0.05;MannWhitney).The present study is the first to compare gingival MACS^+ and MACS- cell populations demonstrating that MACS^+ cells,in contrast to MACS- cells,harbour stem/progenitor cell characteristics.This study also validates the effectiveness of the STRO-l/MACS^+technique for the isolation of gingival stem/progenitor cells.Human free gingival margin-derived STRO-1/MACS^+ cells are a unique renewable source of multipotent stem/progenitor cells. | Karim M Fawzy El-Sayed Sebastian Paris Christian Graetz Neemat Kassem Mohamed Mekhemar Hendrick Ungefroren Fred Fndrich Christof Drfer | 2015 | International Journal of Oral Science2015，7，2： | 6 |
| 2 | Influence of heart rate on vessel visibility in noninvasive coronary angiography using new multislice computed tomography显示文摘 | Stephen Schroeder Andreas F Kopp Axel Kuettner Christof Burgstahler Christian Herdeg Martin Heuschmid Andreas Baumbach Claus D Claussen Karl R Karsch Ludger Seipel | 2002 | Clinical Imaging2002，，2： | 2 |
| 3 | Hypertonic hyperoncotic solutions reduce the release of cardiac troponin Ⅰ and S-100 after successful cardiopulmonary resuscitation in pigs 显示文摘 | Heiner K Christof D Christoph J | 2002 | Anesth Analg2002，95，： | 1 |
| 4 | Oil the or- igin of the extracellular action potential waveform: A modeling study显示文摘 | GOLD C HENZE D A CHRISTOF K | 2006 | Journal of Neurophysiology2006，95，5： | 1 |
| 5 | Dual antibiotic loaded bone cement in patients at high infection risks in arthroplasty: Rationale of use for prophylaxis and scientific evidence显示文摘In view of the demographic changes and projected increase of arthroplasty procedures worldwide,the number of prosthetic joint infection cases will naturally grow.Therefore,in order to counteract this trend more rigid rules and a stricter implementation of effective preventive strategies is of highest importance.In the absence of a'miracle weapon'priorities should lie in evidence-based measures including preoperative optimization of patients at higher infection risks,the fulfilment of strict hygiene rules in the operating theatre and an effective antibiotic prophylaxis regimen.Instead of a'one size fits all'philosophy,it has been proposed to adjust the antibiotic prophylaxis protocol to major infection risks taking into account important patient-and procedure-related risk factors.A stronger focus on the local application mode via use of high dose dual antibioticloaded bone cement in such risk situations may have its advantages and is easy to apply in the theatre.The more potent antimicrobial growth inhibition in vitro and the strong reduction of the prosthetic joint infection rate in risk for infection patients with aid of dual antibiotic-loaded bone cement in clinical studies align with this hypothesis. | Christof Ernst Berberich Jérome Josse Frédéric Laurent Tristan Ferry | 2021 | World Journal of Orthopedics2021，12，3： | 1 |
| 6 | Image Signature: Highlighting Sparse Salient Regions 显示文摘 | Hou X D Jonathan H Christof K | 2012 | IEEE Trans Pattern Anal2012，34，1： | 1 |
| 7 | Image Signature: Highlighting Sparse Salient Regions 显示文摘 | Hou X D Jonathan H Christof K | 2012 | IEEE Transactions on Pattern Analysis and Machine Intelligence2012，34，1： | 1 |
| 8 | Erythropoietin mRNA expression in human fetal and neonatal tissue 显示文摘 | Christof D Hubert F Patricia F | 1998 | Blood1998，92，： | 1 |
| 9 | Image signature:highlighting sparse salient regions显示文摘 | HOU X D JONATHAN Harel CHRISTOF Koch | 2012 | IEEE TPAMI2012，34，1： | 1 |
| 10 | Operational modal analysis in the presence of harmonic excitations by the use of transmissibility measurements 显示文摘 | CHRISTOF D GERT D S STEVE V | 2009 | Mechanical Systems and Signal Processing2009，23，： | 1 |
| 11 | Bridging the Adoption Gap Developing a Roadmap for Trading in Grids显示文摘 | Neumann D Christof Weinhardt J S | 2008 | Electronic Markets2008，18，1： | 1 |
| 12 | Monitoring resonant frequencies and damping values of an offshore wind turbine in parked conditions 显示文摘 | Christof D Wout W Mahmoud E | 2014 | IET Renewable Power Generation2014，8，4： | 1 |
| 13 | Influence of heart rate on vessel visibility in noninvasive coronary angiography using new multislice computed tomography显示文摘 | Stephen Schroeder Andreas F Kopp Axel Kuettner Christof Burgstahler Christian Herdeg Martin Heuschmid Andreas Baumbach Claus D Claussen Karl R Karsch Ludger Seipel | 2002 | Clinical Imaging2002，，2： | 1 |
| 14 | Capsid protein- mediated recruitment of host DnaJ-Like proteins is required forPotato virus Y infection in Tobacco plants显示文摘 | Daniel H Annette T M Christof D | 2007 | Journal of Virology2007，81，11： | 1 |
| 15 | Wideningand Reconstruction of the Kennedy-bridge in BonnGermany显示文摘 | CHRISTOF D UWE H BERND T | 2011 | Stahlbau2011，80，3： | 1 |
| 16 | Model Predictive Pulse PatternControl for the Five-level Active Neutral-point-clampedInverter显示文摘 | Nikolaos Oikonomou Christof Gutscher Petros Karaman-akos Frederick D | 2013 | IEEE Trans on Industrial Applications2013，28，6： | 1 |
| 17 | Hypertonic hyperoncotic solations reduce the release of cardiac troponin Ⅰ and S - 100 after successful cardiopulmonary resuscitation in pigs 显示文摘 | Heiner K Christof D Christoph J | 2002 | Anesth Analg2002，95，： | 1 |
| 18 | Dealing with periodical loads and harmonics in operational modal analysis using time-varying transmissibility functions显示文摘 | Wout W John L Christof D | 2014 | Mechanical Systems and Signal Processing2014，，49： | 1 |
| 19 | Polymerse chain reaction in the detection of micrometastasises显示文摘 | CHRISTOF J ROTERN D | 2000 | J Urol2000，6，1： | 1 |
| 20 | Erythropoietin mRNA expression in human fetal and neonatal tissue 显示文摘 | Christof D Hubert F Patricia F | 1998 | Blood1998，92，： | 1 |
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