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| 1 | 黑曲霉葡萄糖氧化酶基因的克隆及其在酵母中的高效表达显示文摘将黑曲霉葡萄糖氧化酶 (GOD)基因重组进大肠杆菌 酵母穿梭质粒pPIC9,转化甲基营养酵母Pichiapasto risGS115 ,构建出GOD的高产酵母工程菌株。在酵母α Factor及AOX1基因启动子和终止信号的调控下 ,黑曲霉GOD在甲基酵母中大量表达并分泌至胞外 ,经甲醇诱导 3~ 4d ,发酵液中的GOD活力可达 30~ 40u mL。SDS PAGE证实GOD在培养物上清中的含量显著高于其它杂蛋白 ,约占胞外蛋白总量的 6 0 %~ 70 % ,经QSepharoseTM FastFlow离子交换柱一步纯化即达电泳纯。重组酵母GOD比活达 42 6 6 3u mg蛋白 ,是商品黑曲霉GOD的 1 6倍。动力学性质分析表明 ,重组酵母GOD的Km 和kcat分别为 38 2 5mmol L和 34 92 6 6s- 1 ,与商品黑曲霉GOD相比 ,具有更高的催化效率。重组酵母GOD的高活力特性可有效提高葡萄糖传感器的线性检测范围。 | 周亚凤 张先恩 刘虹 张成刚 Anthony E G Cass | 2001 | 生物工程学报2001，17，4： | 25 |
| 2 | Organic molecular tracers for particulate air pollution sources 显示文摘 | 1998 | 1998 | Trends in Analytical Chemistry1998，17，6： | 1 |
| 3 | Organic molecular tracers for particulate air pollution sources 显示文摘 | Cass G R | 1998 | Trends in Analytical Chemistry1998，17，6： | 1 |
| 4 | Ferrocene-mediated enzyme electrode for amperometric determination of glucose显示文摘 | Cass A Davis G Francis G | 1984 | Anal Chem1984，56，4： | 1 |
| 5 | Organic molecular tracers for particulate air pollution sources显示文摘 | Cass G R | 1998 | Trends Analytical Chemistry1998，17，6： | 1 |
| 6 | Source apportionment of wintertime gas-phase and particle-phase air pollutants using organic compounds as tracers 显示文摘 | Schauer J J Cass G R | 2000 | Environ Sci Technol2000，34，： | 1 |
| 7 | Highly polar organic compounds present in meat smoke显示文摘 | Nolte C G Schauer J J Cass G R | 1999 | Environment Science Technology1999，33，19： | 1 |
| 8 | Measurement of aerosol radiative, physical and chemical properties in Beijing during June, 1999 显示文摘 | Beigin M H Cass G R Xu J | 2001 | Journal of Geophysics Research2001，106，17： | 1 |
| 9 | Effects ofsubconjunctival bevacizumab on comeal neovascu-larization :results of a prospective study 显示文摘 | Benayoun Y Adenis JP Casse G | 2012 | Cornea2012，31，8： | 1 |
| 10 | Gas-phase and particle-phase organic compounds emitted from motor vehicle traffic in a Los Angeles roadway tunnel 显示文摘 | Fraser M P Cass G R Simoneit B R T | 1998 | Environmental Science and Technology1998，32，14： | 1 |
| 11 | Chemical characterization of fine particle emissions from fireplace combustion of woods grown in the northeastern United States显示文摘 | Fine P M Cass G R Simoneit B R T 2001 | 2001 | Environ Sci Technol2001，35，13： | 1 |
| 12 | Chemical composition of emissions from urban sources of fine aerosols 显示文摘 | Markowski G R Cass G R 1991 | 1991 | Environ Sci Technol1991，25，： | 1 |
| 13 | Chemical characterization of fine particle emissions from the fireplace combustion of woods grown in the southern United States显示文摘 | Fine P M Cass G R Simoneit B R T 2002 | 2002 | Environ Sci Technol2002，36，7： | 1 |
| 14 | Measurements of particle deposition rates inside southern California museums显示文摘 | Ligocki M P Liu H I H Cass G R | 1990 | Aerosol Science and Technology1990，13，1： | 1 |
| 15 | Chemical composition of emissions from urban sources of fine organic aerosol 显示文摘 | Hildemann L M Markowski G R Cass G R | 1991 | Environ Sci Technol1991，25，4： | 1 |
| 16 | Gas 2 phase and particle two-phase organic compounds emitted from motor vehicle traffic in a Los Angeles roadway tunnel显示文摘 | FRASER M CASS G SIMONEIT B | 1998 | Environmental Science & Technology1998，，32： | 1 |
| 17 | Source Apportionment of Wintertime GasPhase and Particle-Phase Air Pollutants Using Organic Compounds as Tracers 显示文摘 | Schauer J J Cass G R | 2000 | Environmental Science and Technology2000，34，： | 1 |
| 18 | Dye sensitization of nanocrystalline titanium dioxide with osmium and ruthenium polypyridyl complexes显示文摘 | SAUV EG CASS M E COIA G | 2000 | J Phys Chem B2000，104，29： | 1 |
| 19 | Source apportionment of wintertime gasphase and particle-phase air pollutants using organic compounds as tracers显示文摘 | Schauer J J Cass G R 2000 | 2000 | Environ Sci Technol2000，34，9： | 1 |
| 20 | Mathematical modelling of indoor aerosol dynamics显示文摘 | Nazaroff W W Cass G R | 1989 | Environmental Science and Technology1989，23，2： | 1 |
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