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| 1 | 北欧湖泊的重金属调查:浓度、地理模式及与临界界限的关系显示文摘1995年秋天,北欧各国同时对本国的湖泊进行了调查,包括俄罗斯西北的科拉半岛。在大约3000个湖泊中,被调查的11种金属(铅、镉、砷、锌、铜、镍、钴、铁、锰、铬、钒)一般浓度较低,并具有明确的地理模式。对于铅、镉、锌和一定程度上钴的分布,远程迁移的空气污染带来的直接和间接影响是最重要的因素。湖中总有机碳(TOC)浓度对于铁和锰,并在一定程度上对于砷、铬和钒是重要的。对于铜和镍,基岩地质概况是最重要的控制因素,但在科拉半岛很多冶炼厂周围地区是个例外,在那里由于当地空气传播的污染,湖泊中铜和镍的浓度很高。基岩和地表的地质概况对于控制砷、钴、铬和钒的浓度也是重要的因素。调查结果表明在北欧国家湖泊中重金属污染在区域尺度上是一个较轻微的生态问题。 | Brit Lisa Skjelkvle Tom Andersen Eirik Fjeld Jaakko Mannio Anders Wilander Kjell Johansson Jens Peder Jensen Tatyana Moiseenko 孙学飞 | 2001 | AMBIO-人类环境杂志2001，30，1： | 11 |
| 2 | Molecular determinants of the antitumor effects of trichostatin A in pancreatic cancer cells显示文摘AIM:To gain molecular insights into the action of the histone deacetylase inhibitor(HDACI) trichostatin-A(TSA) in pancreatic cancer(PC) cells.METHODS:Three PC cell lines,BxPC-3,AsPC-1 and CAPAN-1,were treated with various concentrations of TSA for def ined periods of time.DNA synthesis was assessed by measuring the incorporation of 5-bromo-2'deoxyuridine.Gene expression at the level of mRNA was quantif ied by real-time polymerase chain reaction.Expression and phosphorylation of proteins was monitored by immunoblotting,applying an infrared imaging technology.To study the role of p38 MAP kinase,the specif ic enzyme inhibitor SB202190 and an inactive control substance,SB202474,were employed.RESULTS:TSA most eff iciently inhibited BrdU incorporation in BxPC-3 cells,while CAPAN-1 cells displayed the lowest and AsPC-1 cells an intermediate sensitivity.The biological response of the cell lines correlated with the increase of histone H3 acetylation after TSA application.In BxPC-3 cells(which are wild-type for KRAS),TSA strongly inhibited phosphorylation of ERK 1/2 and AKT.In contrast,activities of ERK and AKT in AsPC-1 and CAPAN-1 cells(both expressing oncogenic KRAS) were not or were only modestly affected by TSA treatment.In all three cell lines,but most pronounced in BxPC-3 cells,TSA exposure induced an activation of the MAP kinase p38.Inhibition of p38 by SB202190 slightly but signif icantly diminished the antiproliferative effect of TSA in BxPC-3 cells.Interestingly,only BxPC-3 cells responded to TSA treatment by a signif icant increase of the mRNA levels of bax,a pro-apoptotic member of the BCL gene family.Finally,in BxPC-3 and AsPC-1 cells,but not in the cell line CAPAN-1,signif icantly higher levels of the cell cycle inhibitor protein p21Waf1 were observed after TSA application.CONCLUSION:The biological effect of TSA in PC cells correlates with the increase of acetyl-H3,p21Waf1,phospho-p38 and bax levels,and the decrease of phosphoERK 1/2 and phospho-AKT. | Elisabeth Emonds Brit Fitzner Robert Jaster | 2010 | World Journal of Gastroenterology2010，16，16： | 5 |
| 3 | Impact of hyperglycemia on autoimmune pancreatitis and regulatory T-cells显示文摘AIM To evaluate the influence of hyperglycemia on the progression of autoimmune pancreatitis.METHODS We induced hyperglycemia by repetitive intraperitoneal(ip) injection of 50 mg/kg streptozotocin in MRL/Mp J mice, which develop autoimmune pancreatitis due to a genetic predisposition. We compared the extent of inflammation(histological score, CD3^+ lymphocytes, CD8^+ T-cells, CD4^+ T-cells, Foxp3^+ T-helper cells) in the pancreas of hyperglycemic and normoglycemic mice. We also analyzed the number of leukocytes, lymphocytes, granulocytes and monocytes in the blood. In addition, we determined the percentage of CD3^+ lymphocytes, CD8^+ T-cells, CD4^+ T-cells, Foxp3^+ T-helper cells, Foxp3^+ CD25^+ T-helper and Foxp3^+T-helper cells in the spleen by flow cytometry.RESULTS Treatment with streptozotocin caused a strong induction of hyperglycemia and a reduction in body weight(P < 0.001). Severe hyperglycemia did not, however, lead to an aggravation, but rather to a slight attenuation of autoimmune pancreatitis. In the pancreas, both the histological score of the pancreas as well as the number of CD3+ lymphocytes(P < 0.053) were decreased by hyperglycemia. No major changes in the percentage of CD8^+ T-cells, CD4^+ T-cells, Foxp3^+ T-helper cells were observed between hyperglycemic and normoglycemic mice. Hyperglycemia increased the numbers of leukocytes(P < 0.001), lymphocytes(P = 0.016), granulocytes and monocytes(P = 0.001) in the blood. Hyperglycemia also moderately reduced the percentage of CD3^+ lymphocytes(P = 0.057), significantly increased the percentage of Foxp3^+ T-helper cells(P = 0.018) and Foxp3^+ CD25^+ T-helper cells(P = 0.021) and reduced the percentage of Foxp3^+T-helper cells(P = 0.034) in the spleen. CONCLUSION Hyperglycemia does not aggravate but moderately attenuates autoimmune pancreatitis, possibly by increasing the percentage of regulatory T-cells in the spleen. | Franz-Tassilo Müller-Graff Brit Fitzner Robert Jaster Brigitte Vollmar Dietmar Zechner | 2018 | World Journal of Gastroenterology2018，24，28： | 3 |
| 4 | CSF total and oligomeric α-Synuclein along with TNF-α as risk biomarkers for Parkinson’s disease: a study in LRRK2 mutation carriers显示文摘Background Asymptomatic carriers of leucine-rich repeat kinase 2(LRRK2)gene mutations constitute an ideal population for discovering prodromal biomarkers of Parkinson’s disease(PD).In this study,we aim to identify CSF candidate risk biomarkers of PD in individuals with LRRK2 mutation carriers.Methods We measured the levels of CSF total-(t-),oligomeric(o-)and phosphorylated S129(pS129-)α-syn,total-tau(tTau),phosphorylated threonine 181 tau(pTau),amyloid-beta 40(Aβ-40),amyloid-beta-42(Aβ-42)and 40 inflammatory chemokines in symptomatic(n=23)and asymptomatic(n=51)LRRK2 mutation carriers,subjects with a clinical diagnosis of PD(n=60)and age-matched healthy controls(n=34).General linear models corrected for age and gender were performed to assess differences in CSF biomarkers between the groups.Markers that varied significantly between the groups were then analyzed using backward-elimination logistic regression analysis to identify an ideal biomarkers panel of prodromal PD.Results Discriminant function analysis revealed low levels of CSF t-α-syn,high levels of CSF o-α-syn and TNF-αbest discriminated asymptomatic LRRK2 mutation carriers from both symptomatic PD and healthy controls.Assessing the discriminative power using receiver operating curve analysis,an area under the curve>0.80 was generated.Conclusions The current study suggests that CSF t-,o-α-syn and TNF-αare candidate risk biomarkers for the detection of PD at the prodromal stage.Our findings also highlight the dynamic interrelationships between CSF proteins and the importance of using a biomarkers’panel approach for an accurate and timely diagnosis of PD. | Nour K.Majbour Jan O.Aasly Eldbjørg Hustad Mercy A.Thomas Nishant N.Vaikath Naser Elkum Wilma D.Jvan de Berg Takahiko Tokuda Brit Mollenhauer Henk W.Berendse Omar M.A.El-Agnaf | 2020 | Translational Neurodegeneration2020，9，2： | 2 |
| 5 | Distinct antifibrogenic effects of erlotinib,sunitinib and sorafenib on rat pancreatic stellate cells显示文摘AIM:To study if three clinically available small molecule kinase inhibitors(SMI),erlotinib,sunitinib and sorafenib,exert antifibrogenic effects on pancreatic stellate cells(PSC)and analyze the basis of their action.METHODS:Cultured rat PSC were exposed to SMI.Cell proliferation and viability were assessed employing 5-bromo-2’-deoxyuridine incorporation assay and flow cytometry,respectively.2-Deoxy-2-[18F]fluoroglucose(18F-FDG)uptake was measured to study metabolic activity.Exhibition of the myofibroblastic PSC phenotype was monitored by immunofluorescence analysis ofα-smooth muscle actin(α-SMA)expression.Levels of mRNA were determined by real-time PCR,while protein expression and phosphorylation were analyzed by immunoblotting.Transforming growth factor-β1 (TGF-β1)levels in culture supernatants were quantified by ELISA.RESULTS:All three SMI inhibited cell proliferation and18F-FDG uptake in a dose-dependent manner and without significant cytotoxic effects.Furthermore,additive effects of the drugs were observed.Immunoblot analysis showed that sorafenib and sunitib,but not erlotinib,efficiently blocked activation of the AKT pathway,while all three drugs displayed little effect on phosphorylation of ERK1/2.Cells treated with sorafenib or sunitinib expressed less interleukin-6 mRNA as well as less collagen type 1 mRNA and protein.Sorafenib was the only drug that also upregulated the expression of matrix metalloproteinase-2 and reduced the secretion of TGF-β1 protein.All three drugs showed insignificant or discordant effects on the mRNA and protein levels ofα-SMA.CONCLUSION:The tested SMI,especially sorafenib,exert inhibitory effects on activated PSC,which should be further evaluated in preclinical studies. | Anne Elsner Falko Lange Brit Fitzner Martin Heuschkel Bernd Joachim Krause Robert Jaster | 2014 | World Journal of Gastroenterology2014，20，24： | 2 |
| 6 | Expression of estrogen receptor-alpha and -beta and progesterone receptor-A and -B in a large cohort of patients with endometrioid endome- trial cancer 显示文摘 | Jongen V Brit J de Jong R | 2009 | Gynecol Oncol2009，12，3： | 1 |
| 7 | Atherosclerotic lesions in lymphoma survivors treated with radiotherapy显示文摘 | Torgeir Wethal B?rd Nedregaard Rune Andersen Alexander Foss? May Brit Lund Anne Günther Stein Kval?y Sophie D. Foss? John Kjekshus | 2013 | Radiotherapy and Oncology2013，，： | 1 |
| 8 | Risk factors for postoperative pneumonia 显示文摘 | Garibaldi RA Brit MR Coleman ML | 1981 | Am J Surg1981，70，： | 1 |
| 9 | Photolysis of uranyl oxalate system 3:photochemical behavior,kinetics- and mechanism in aqueous-solution显示文摘 | Brits A G Vaneldik R Vandenberg J A | 1977 | J Inorg Nucl Chem1977，39，7： | 1 |
| 10 | Photolysis of uranyl formic acid formate system in acidic aqueous solution显示文摘 | Brits A G Vaneldik R Vandenberg J A | 1978 | Inorg Chim Acta1978，30，1： | 1 |
| 11 | Procoagulant and profibrinolytie activities of cryopreserved human monocytes 显示文摘 | Liv T NOsnes Ase Brit Westvik Peter Kierulf | 1994 | Thrombosis Research1994，76，4： | 1 |
| 12 | Nanage ment of duodenal injuries显示文摘 | ASENSIO J A FELICIANO D V BRIT L D | 1993 | Our Prob In Surg1993，30，： | 1 |
| 13 | CSF amyloid-β-peptides in Alzheimer's disease,dementia with Lewy bodies and Parkinson's disease dementia显示文摘 | Brit M Hermann E | 2006 | Brain2006，129，： | 1 |
| 14 | The temporalis muscle flap for head and neck reconstruction 显示文摘 | Brit BD Antonyshyn O Gruss JS | 1987 | J Otolaryngol1987，16，3： | 1 |
| 15 | Polymer显示文摘 | Wright P V Brit | 1975 | (7):3191975，，7： | 1 |
| 16 | Reversion of multidrug resistance with polyalkylcyanoacrylate nanoparticles: Towards a mechanism of action显示文摘 | de Verdi~re A Brit | 1997 | British Journal of Cancer1997，76，2： | 1 |
| 17 | Locating multiple optima using particle swarm optimization显示文摘 | Brits R Engelbrecht A P van den Bergh F | 2007 | Appl Math Comput2007，189，2： | 1 |
| 18 | A quantification of short-term macroaggregate dynamics: influences of wheat residue input and texture显示文摘 | Steven De Gryze Johan Six Cynthia Brits Roel Merckx | 2004 | Soil Biology and Biochemistry2004，，1： | 1 |
| 19 | A local composition mode1 for the excess Gibbs energy of aqueous electrolyte systems Part I:single Solvent,single completely dissociated electrolyte system显示文摘 | CHEN C C BRIT H I BOSTON J F | 1982 | AIChE J1982，28，4： | 1 |
| 20 | Locating multiple optima using particle swarm optimization显示文摘 | Brits R Engelbrecht A P van den Bergh F | 2007 | Applied Mathematics and Computation2007，189，： | 1 |
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