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| 1 | The Isogene 1-Deoxy-D-Xylulose 5-Phosphate Synthase 2 Controls Isoprenoid Profiles, Precursor Pathway Allocation, and Density of Tomato Trichomes显示文摘植物 isoprenoids 从先锋被形成由 mevalonate (MVA ) 综合了在 cytosol 或由 methyl-D-erythritol 4 磷酸盐(MEP ) 的小径在质体的小径。尽管先锋的一些交换发生, cytosolic sesquiterpenes 被假定主要源于 MVA,当 plastidial monoterpenes 从 MEP 先锋优先地被生产时。另外的复杂性在 MEP 小径的第一步产生,它被二分叉的 1-deoxy-D-xylulose 5 磷酸盐 synthase isoforms 典型地催化(DXS1, DXS2 ) 。在西红柿(茄属 lycopersicum ) , SlDXS1 基因无所不在地在而 SlDXS2 抄本充满仅仅很少纸巾,成熟的水果期间与高水平被表示,包括年轻叶子,花瓣,和孤立的毛状体。SlDXS2 表示的特定的下面规定被 RNA 干扰在转基因的植物执行调查反馈机制。SlDXS2 下面规定在在二 sesquiterpenes 的 monoterpene -phellandrene 和毛状体的增加导致了减少。而且,进剩余 monoterpenes 并且进 sesquiterpenes 的导出 MVA 的先锋的加入是由 13C 的比较决定了到 12C 天赋同位素比率被提高。SlDXS1 的一条补偿起来规定没被观察。也展出的下面调整的线增加了毛状体密度并且由喂叶的 Spodoptera littoralis 毛虫显示出更少的损坏。结果在 isoprenoid 先锋分配在 modulating isoprenoid 新陈代谢和显著粘性揭示 DXS2 的新奇、非冗余的角色。 | Heike Paetzold Stefan Garms Stefan Bartram Jenny Wieczorek Eva-Maria Uros-Gracia Manuel Rodriguez-Concepcion Wilhelm Boland Dieter Strack Bettina Hause Michael H. Walter | 2010 | Molecular Plant2010，3，5： | 11 |
| 2 | Mutation of the Arabidopsis Calmodulin-Like Protein CML37 Deregulates the Jasmonate Pathway and Enhances Susceptibility to Herbivory显示文摘在整个他们的生活,植物被各种各样的不能生活、关於生命的压力因素质问。在那些之中是从食草的昆虫的攻击。位于草食动物和随后的信号 transduction 的察觉下面的分子的机制很好没被理解。作为第二个送信人,在细胞内部的 Ca 2+ 层次的流动在调停起一个关键作用压力反应小径。Ca 2+ 信号被象象 calmodulin 一样蛋白质(电流型逻辑) 那样的 Ca 2+ 传感器蛋白质译码。这里,我们证明 recombinant CML37 在 vitro 象一个 Ca 2+ 传感器一样表现并且在 Arabidopsis, AtCML37 被机械伤害以及由群袭与通晓各方面知识经验丰富的人的幼虫导致鳞翅类的草食动物 Spodoptera littoralis。CML37 的功能的损失导致了建议 CML37 是一个积极防卫管理者的幼虫的更好的喂表演。尽管在 jasmonates 的累积的重要减小被观察,在象 glucosinolates 或 flavonoids 那样的第二等的代谢物的导致 herbivory 的变化都没在 cml37 植物被检测,由于 JAR1 mRNA 和细胞的酶活动的减少的表示。因而, jasmonate 应答的基因的表示也被减少。总结,我们的结果建议 Ca 2+ 传感器蛋白质, CML37,在在 herbivory,连接 Ca 2+ 并且 jasmonate 发信号期间发信号的 Ca 2+ 作为一个积极管理者工作。 | Sandra S. Scholz Jyothilakshmi Vadassery Monika Heyer Michael Reichelt Kyle W. Bender Wayne A. Snedden Wilhelm Boland Axel Mithofer | 2014 | Molecular Plant2014，7，12： | 11 |
| 3 | 乳腺癌发生模型MCF10各细胞系中APC基因启动子区甲基化及mRNA表达检测显示文摘目的探讨乳腺癌发生模型MCF10中抑癌基因APC启动子区甲基化状态及其对mRNA表达的影响。方法应用甲基化特异性聚合酶链反应(MSP)及双亚硫酸钠基因测序技术检测MCF10模型的乳腺增生细胞系MCF10A、癌前细胞系MCF10AT、导管内癌细胞系MCF10DC IS.com、浸润癌细胞系MCF10CA1 a、MCF10CA1d、MCF10CA1h及经典乳腺癌细胞系MCF-7和正常乳腺组织中APC基因启动子1A甲基化状态,然后用逆转录聚合酶链反应(RT-PCR)和实时PCR技术检测上述样品的mRNA表达水平。结果在MCF10模型的增生细胞系、癌前细胞系、导管内癌细胞系、浸润癌细胞系中,APC基因启动子1A处于低甲基化状态;与正常乳腺组织相比,各细胞系APC基因mRNA表达无明显减少,MCF10AT、MCF10CA1d、MCF10CA1h、MCF10DC IS.com的mRNA表达分别减少0.27、0.96、1.78、2.70、2.03倍,MCF10A和MCF-7分别增加0.02和0.33倍)。结论MCF10模型中乳腺癌的发生发展过程与APC基因启动子区异常甲基化无关。 | 杨举伦 David Klinkebiel Michael J Boland Lin Tang Judith K Christman | 2006 | 中华病理学杂志2006，35，1： | 7 |
| 4 | Nonalcoholic steatohepatitis severity is defined by a failure in compensatory antioxidant capacity in the setting of mitochondrial dysfunction显示文摘AIM To comprehensively evaluate mitochondrial(dys) function in preclinical models of nonalcoholic steatohepatitis(NASH).METHODS We utilized two readily available mouse models of nonalcoholic fatty liver disease(NAFLD) with or without progressive fibrosis: Lep^(ob)/Lep^(ob)(ob/ob) and FATZO mice on high trans-fat, high fructose and high cholesterol(AMLN) diet. Presence of NASH was assessed using immunohistochemical and pathological techniques, and gene expression profiling. Morphological features of mitochondria were assessed via transmission electron microscopy and immunofluorescence, and function was assessed by measuring oxidative capacity in primary hepatocytes, and respiratory control and proton leak in isolated mitochondria. Oxidative stress was measured by assessing activity and/or expression levels of Nrf1, Sod1, Sod2, catalase and 8-OHdG. RESULTS When challenged with AMLN diet for 12 wk, ob/ob and FATZO mice developed steatohepatitis in the presence of obesity and hyperinsulinemia. NASH development was associated with hepatic mitochondrial abnormalities, similar to those previously observed in humans, including mitochondrial accumulation and increased proton leak. AMLN diet also resulted in increased numbers of fragmented mitochondria in both strains of mice. Despite similar mitochondrial phenotypes, we found that ob/ob mice developed more advanced hepatic fibrosis. Activity of superoxide dismutase(SOD) was increased in ob/ob AMLN mice, whereas FATZO mice displayed increased catalase activity, irrespective of diet. Furthermore, 8-OHd G, a marker of oxidative DNA damage, was significantly increased in ob/ob AMLN mice compared to FATZO AMLN mice. Therefore, antioxidant capacity reflected as the ratio of catalase:SOD activity was similar between FATZO and C57 BL6 J control mice, but significantly perturbed in ob/ob mice. CONCLUSION Oxidative stress, and/or the capacity to compensate for increased oxidative stress, in the setting of mitochondrial dysfunction, is a key factor for development of hepatic injury and fibrosis in these mouse models. | Michelle L Boland Stephanie Oldham Brandon B Boland Sarah Will Jean-Martin Lapointe Silvia Guionaud Christopher J Rhodes James L Trevaskis | 2018 | World Journal of Gastroenterology2018，24，16： | 7 |
| 5 | 乳腺癌发生模型MCF10各阶段细胞系中TIMP3基因启动子区甲基化分析显示文摘目的探讨抑癌基因TIMP3失活与乳腺癌发生和进展的关系。方法用甲基化特异性PCR技术和亚硫酸盐测序技术检测乳腺癌发生模型MCF10的增生细胞系MCF10A、癌前细胞系MCF10AT、导管内癌细胞系MCF10DCIS.com、浸润癌细胞系MCF10CA1a和转移癌细胞系MCF10CA1d、MCF10CA1h中TIMP3启动子区甲基化状态。结果甲基化特异性PCR分析显示,在上述各细胞系中,TIMP3启动子区均呈高度甲基化状态。亚硫酸盐测序显示,在上述各细胞系中,测序区内的68个CG位点几乎全部发生了甲基化,且甲基化累及了绝大部分等位基因。结论TIMP3基因启动子区甲基化在乳腺癌的发生和进展中起重要作用,可能成为早期诊断乳腺癌和判断乳腺癌预后的分子生物学标记。 | 杨举伦 David Klinkebiel Michael J Boland Lin Tang Judith K Christman | 2006 | 临床与实验病理学杂志2006，22，1： | 7 |
| 6 | MicroRNA-200c modulates epithelial-to-mesenchymal transition (EMT) in human colorectal cancer metastasis显示文摘 | Keun Hur Yuji Toiyama Masanobu Takahashi Francesc Balaguer Takeshi Nagasaka Junichi Koike Hiromichi Hemmi Minoru Koi C Richard Boland Ajay Goel | 2013 | Gut2013，，9： | 6 |
| 7 | 乳腺癌发生过程中NOEY2基因启动子区甲基化及mRNA表达显示文摘目的探讨乳腺癌发生过程中抑癌基因NOEY2启动子区甲基化状态及其对mRNA表达的影响。方法应用甲基化特异性PCR及双亚硫酸钠基因测序技术检测MCF10模型中乳腺增生细胞系MCF10A、癌前细胞系MCF10AT、导管内癌细胞系MCF10DC IS.com、浸润癌细胞系MCF10CA1 a、MCF10CA1d、MCF10CA1h及正常乳腺组织中NOEY2基因启动子区CpG岛I甲基化状态,然后用RT-PCR和实时PCR技术检测上述样品的mRNA表达水平。结果MCF10模型的增生细胞系、癌前细胞系、导管内癌细胞系、浸润癌细胞系均发生该基因启动子区CpG岛I高度甲基化;与正常乳腺组织相比,上述细胞系mRNA表达显著减少。结论NOEY2基因启动子区高度甲基化及相应的mRNA表达减少是乳腺癌发生过程中的早期事件,与乳腺癌发生有关,可能成为早期诊断乳腺癌的潜在分子生物学标记。 | 杨举伦 David Klinkebiel Michael J Boland Lin Tang Judith K Christman | 2005 | 临床与实验病理学杂志2005，21，5： | 6 |
| 8 | Involvement of sweet pepper Ca LOX2 in jasmonate-dependent induced defence against Western flower thrips显示文摘Insect herbivory can seriously hinder plant performance and reduce crop yield. Thrips are minute cell-content-feeding insects that are important vectors of viral plant pathogens, and are serious crop pests. We investigated the role of a sweet pepper (Capsicum annuum) lipoxygenase gene, CaLOX2, in the defense of pepper plants against Western flower thrips (Frankliniella occidentalis). This was done through a combination of in-silico, transcrip-tional, behavioral andchemicalanalyses.Our datashowthat CaLOX2 is involved in jasmonic acid (JA) biosynthesis and mediates plant resistance. Expression of the JA-related marker genes, CaLOX2 and CaPIN II, was induced by thrips feeding. Silencing of CaLOX2 in pepper plants through virus-induced gene silencing (VIGS) resulted in low levels of CaLOX2 transcripts, as well as significant reduction in the accumulation of JA, and its derivatives, upon thrips feeding compared to control plants. CaLOX2-silenced pepper plants exhibited enhanced susceptibility to thrips. This indicates that CaLOX2 mediates JA-dependent signaling, resulting in defenseagainstthrips. Furthermore,exogenous application of JA to pepper plants increased plant resistance to thrips, constrained thrips population development and made plants less attractive to thrips. Thus, a multidisciplinary approach shows that an intact lipoxygenase pathway mediates various components of sweet pepper defense against F. occidentalis. | Sandeep J Sarde Klaas Bouwmeester Jhon Venegas-Molina Anja David Wilhelm Boland Marcel Dicke | 2019 | Journal of Integrative Plant Biology2019，61，10： | 6 |
| 9 | 手机:医院不该忽略的战略工具显示文摘其实很多时候,往往是日常细节的改变,常用工具的重新定位,就实现了医院管理中的理念和战略,并对医院管理产生了根本性的,甚至革命性的影响所以说,平时小的,常见的事物里并非不能产生管理创新。 | Peter Boland 符策慧(编译) | 2006 | 中国医疗前沿2006，，7： | 4 |
| 10 | The Virulence Plasmid of Yersinia, an Antihost Genome 显示文摘 | Cornelis GR Boland A Boyd AP | 1998 | Microbiology and Molecular Biology Reviews1998，62，： | 4 |
| 11 | Chemical and biological transformations for detoxification of trichothecene mycotoxins in human and animal food chains: a review显示文摘 | Jianwei He Ting Zhou J. Christopher Young Greg J. Boland Peter M. Scott | 2009 | Trends in Food Science & Technology2009，，2： | 3 |
| 12 | Serum miR-200c Is a Novel Prognostic and Metastasis-Predictive Biomarker in Patients With Colorectal Cancer显示文摘 | Yuji Toiyama Keun Hur Koji Tanaka Yasuhiro Inoue Masato Kusunoki C. Richard Boland Ajay Goel | 2014 | Annals of Surgery2014，，4： | 3 |
| 13 | 乳腺癌发生模型MCF10抑癌基因NOEY2启动子区甲基化及mRNA表达显示文摘 | 杨举伦 David Klinkebiel Michael J Boland Lin Tang Judith K Christman | 2005 | 中华病理学杂志2005，34，3： | 3 |
| 14 | Towards a standard diet-induced and biopsy-confirmed mouse model of non-alcoholic steatohepatitis: Impact of dietary fat source显示文摘BACKGROUND The trans-fat containing AMLN(amylin liver non-alcoholic steatohepatitis,NASH)diet has been extensively validated in C57BL/6J mice with or without the Lep^ob/Lep^ob(ob/ob)mutation in the leptin gene for reliably inducing metabolic and liver histopathological changes recapitulating hallmarks of NASH.Due to a recent ban on trans-fats as food additive,there is a marked need for developing a new diet capable of promoting a compatible level of disease in ob/ob and C57BL/6J mice.AIM To develop a biopsy-confirmed mouse model of NASH based on an obesogenic diet with trans-fat substituted by saturated fat.METHODS Male ob/ob mice were fed AMLN diet or a modified AMLN diet with trans-fat(Primex shortening)substituted by equivalent amounts of palm oil[Gubra amylin NASH,(GAN)diet]for 8,12 and 16 wk.C57BL/6J mice were fed the same diets for 28 wk.AMLN and GAN diets had similar caloric content(40%fat kcal),fructose(22%)and cholesterol(2%)level.RESULTS The GAN diet was more obesogenic compared to the AMLN diet and impaired glucose tolerance.Biopsy-confirmed steatosis,lobular inflammation,hepatocyte ballooning,fibrotic liver lesions and hepatic transcriptome changes were similar in ob/ob mice fed the GAN or AMLN diet.C57BL/6J mice developed a mild to moderate fibrotic NASH phenotype when fed the same diets.CONCLUSION Substitution of Primex with palm oil promotes a similar phenotype of biopsyconfirmed NASH in ob/ob and C57BL/6J mice,making GAN diet-induced obese mouse models suitable for characterizing novel NASH treatments. | Michelle L Boland Denise Oro Kirstine S T■lb■l Sebastian T Thrane Jens Christian Nielsen Taylor S Cohen David E Tabor Fiona Fernandes Andrey Tovchigrechko Sanne S Veidal Paul Warrener Bret R Sellman Jacob Jelsing Michael Feigh Niels Vrang James L Trevaskis Henrik H Hansen | 2019 | World Journal of Gastroenterology2019，25，33： | 3 |
| 15 | Adrenal masses:characterization with delayed contrast-enhanced CT 显示文摘 | Boland GWL Hahn PF Pena C | 1997 | Radiology1997，202，： | 2 |
| 16 | The History of Lynch Syndrome显示文摘 | C. Richard Boland Henry T. Lynch | 2013 | Familial Cancer2013，，2： | 2 |
| 17 | 智慧城市:数字技术打造宜居家园显示文摘历经十年的探索之后,智慧城市的发展走进了一个新阶段。尽管数字技术只是打造美好城市的其中一项工具,但它的确是近年来最有成效的办法之一。本报告分析了当前全球智慧城市采用的近60项应用,发现这些应用可将城市生活质量的指标提高10%~30%。报告同时发现,即便是走在世界最前沿的智慧城市也仍然具有巨大的进步空间。 | 华强森 吕文博 Jaana Remes Brodie Boland | 2018 | 科技中国2018，，10： | 2 |
| 18 | Neurologic and neuropathologic outcome after middle cerebral artery occlusion in rats显示文摘 | Persson L Hardemark HG Bolander HG | 1989 | Stroke1989，20，5： | 2 |
| 19 | Chest compression force of trained and untrained CPR rescuers显示文摘 | GEDDES L A BOLAND M K TALEYARKHAN P R | 2007 | Cardiovasc Eng2007，7，： | 1 |
| 20 | The relationship between syntactic processes in sentence comprehension显示文摘 | | 1997 | Language and Cognitive Processes1997，，4： | 1 |
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