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| 1 | Plant Genome Editing Using FnCpfl and LbCpf Nucleases at Redefined and Altered PAM-Sites显示文摘 | ZhaohuiZhong Yingxiao Zhang Qi You XU Tang Qiurong Ren Shishi Liu Lijia Yang Yan Wang Xiaopei Liu Binglin Liu Tao Zhang Xuelian Zheng Ysa Le Yong Zhang Yiping Qi | 2018 | Molecular Plant2018，11，7： | 10 |
| 2 | Targeted Mutagenesis of NAC Transcription Factor Gene,OsN AC041,Leading to Salt Sensitivity in Rice显示文摘Salinity is a major abiotic stress factor that seriously affects plant growth. Many genes are involved in the response to salt stress with various metabolism pathways. A number of plant transcription factor family genes have been found to be involved in the salt stress response, and NAM, ATAF and CUC(NAC) transcription factors are thought to act as active regulators during abiotic stress, especially salt stress. In this study, we detected a rice NAC transcription factor coding gene, OsNAC041, and confirmed that it influenced the germination of seeds under salt stress and salt tolerance of plants. OsNAC041 was primarily expressed in the leaves and located in the nucleus. Furthermore, the CRISPR/Cas9 method was used to obtain a targeted osnac041 mutant, of which the plant height was higher than that of the wild-type, showing increased salt sensitivity. Moreover, RNA-seq analysis revealed a number of differentially expressed genes(DEGs) involved in several important signaling pathways in the osnac041 mutant. Subsequently, Kyoto Encyclopedia of Genes and Genomes annotation also revealed differential expression of DEGs associated with mitogen-activated protein kinase signaling, peroxisome, eukaryotictype ABC transporters, photosynthesis and plant hormones, which are involved in stress-related signaling pathways. Overall, our study suggested that OsNAC041 was involved in the salt stress response in rice. These findings not only provide empirical evidence of OsNAC041 function, but also provide new insight into its potential application in rice resistance breeding. | WANG Bo ZHONG Zhaohui ZHANG Huanhuan WANG Xia LIU Binglin YANG Lijia HAN Xiangyan YU Deshui ZHENG Xuelian WANG Chunguo SONG Wenqin CHEN Chengbin ZHANG Yong | 2019 | Rice science2019，26，2： | 8 |
| 3 | Efficient elimination of multidrug-resistant bacteria using copper sulfide nanozymes anchored to graphene oxide nanosheets显示文摘Antibacterial nanomaterials have attracted growing interest for bacterial infection therapy.However,most nanomaterials eliminate bacteria either physically or chemically,which hampers their efficacy when dealing with multidrug-resistant bacteria.To overcome this,we integrated copper sulfide(CuS)nanoparticles with active graphene oxide nanosheets(GO NSs)to synthesize a superior nanocomposite(CuS/GO NC)that acts both physically and chemically on the bacteria.CuS/GO NC was produced using a facile hydrothermal method,whereby the CuS nanoparticles grew and were uniformly dispersed on the GO NSs in situ.We found that the CuS/GO NC possesses a unique needle-like morphology that physically damages the bacterial cell membrane.CuS/GO NC also exhibits high oxidase-and peroxidase-like activity,ensuring efficient generation of the reactive oxygen species•OH from H2O2,which kills bacteria chemically.These features endow the CuS/GO NC with excellent antibacterial capabilities to kill multidrug-resistant bacteria such as methicillin-resistant Staphylococcus aureus(MRSA)with only a single dose.Additionally,it was found that the CuS/GO NC accelerated the healing of infected wounds in vivo owing to its good biocompatibility as well as facilitation of cell migration and collagen secretion.This study provides a new strategy to combine the physical and chemical antibacterial modes of nanomaterials to develop more effective therapies to combat multidrug-resistant bacterial infections. | Wanshun Wang Binglin Li Huili Yang Zefeng Lin Lingling Chen Zhan Li Jiayuan Ge Tao Zhang Hong Xia Lihua Li Yao Lu | 2020 | Nano Research2020，13，8： | 8 |
| 4 | Effect of N fertilization rate on soil alkalihydrolyzable N, subtending leaf N concentration,fiber yield, and quality of cotton显示文摘Soil alkali-hydrolyzable nitrogen, which is sensitive to N fertilization rate, is one of the indicators of soil nitrogen supplying capacity. Two field experiments were conducted in Dongtai(120°19″ E, 32°52″ N), Jiangsu, China in 2009 and Dafeng(120°28″ E, 33°12″ N), Jiangsu province, China in 2010. Six nitrogen rates(0, 150, 300, 375, 450, and 600 kg ha^(-1)) were used to study the effect of N fertilization rate on soil alkali-hydrolyzable nitrogen content(SAHNC), subtending leaf nitrogen concentration(SLNC), yield, and fiber quality. In both Dongtai and Dafeng experiment station, the highest yield(1709 kg ha^(-1)), best quality(fiber length 30.6 mm, fiber strength 31.6 c N tex^(-1), micronaire 4.82), and highest N agronomic efficiency(2.03 kg kg^(-1)) were achieved at the nitrogen fertilization rate of 375 kg ha^(-1). The dynamics of SAHNC and SLNC could be simulated with a cubic and an exponential function,respectively. The changes in SAHNC were consistent with the changes in SLNC. Optimal average rate(0.276 mg day^(-1)) and duration(51.8 days) of SAHNC rapid decline were similar to the values obtained at the nitrogen rate of 375 kg ha^(-1)at which cotton showed highest fiber yield, quality, and N agronomic efficiency. Thus, the levels and strategies of nitrogen fertilization can affect SAHNC dynamics. The N fertilization rate that optimizes soil alkali-hydrolyzable nitrogen content would optimize the subtending leaf nitrogen concentration and thereby increase the yield and quality of the cotton fiber. | Binglin Chen Hongkun Yang Weichao Song Chunyu Liu Jiao Xu Wenqing Zhao Zhiguo Zhou | 2016 | The Crop Journal2016，4，4： | 8 |
| 5 | New Method to Measure the Fill Level of the Ball Mill I——Theoretical Analysis and DEM Simulation显示文摘The accurate measurement of the fill level in the ball mill has not been resolved because of the interplay of many variable factors, which led the mill to be operated under the uneconomical condition and lost a lot of energy. At present, some methods, such as vibration method and acoustic method, have been applied for measuring the fill level by the researchers. Aiming at the problem of the traditional methods for measuring the fill level, that is, the feature variables of the fill level suffer the influences of the ball load and the water content of the coal, a novel method to measure the fill level is proposed and a possible relation between the fill level and the angular position of the maximum vibration point on the mill shell is investigated. The angular positions of the maximum vibration point on the mill shell for different fill level cases are calculated theoretically under two assumptions, respectively. Meanwhile the charge motions of the mill for different fill level cases are simulated with the discrete element method (DEM). And the simulation results are verified by comparing the motion trajectories of steel balls and power draft of the mill. The simulated movement trajectories of the outmost layer steel balls in the mill are monitored and analyzed to obtain the angular positions of the maximum vibration point on the mill shell. Both the results of the theoretical calculation and the 3D DEM simulation show that the position of the maximum vibration point on the mill shell moves to a lower angular positions as the fill level decreasing, which provides a new idea for measuring the fill level accurately. | HUANG Peng JIA Minping ZHONG Binglin | 2010 | Chinese Journal of Mechanical Engineering2010，23，4： | 4 |
| 6 | Assessment of organic compost and biochar in promoting phytoremediation of crude-oil contaminated soil using Calendula officinalis in the Loess Plateau, China显示文摘The Loess Plateau,located in Gansu Province,is an important energy base in China because most of the oil and gas resources are distributed in Gansu Province.In the last 40 a,ecological environment in this region has been extremely destroyed due to the over-exploitation of crude-oil resources.Remediation of crude-oil contaminated soil in this area remains to be a challenging task.In this study,in order to elucidate the effects of organic compost and biochar on phytoremediation of crude-oil contaminated soil(20 g/kg)by Calendula officinalis,we designed five treatments,i.e.,natural attenuation(CK),planted C.officinalis only(P),planted C.officinalis with biochar amendment(PB),planted C.officinalis with organic compost amendment(PC),and planted C.officinalis with co-amendment of biochar and organic compost(PBC).After 152 d of cultivation,total petroleum hydrocarbons(TPH)removal rates of CK,P,PB,PC and PBC were 6.36%,50.08%,39.58%,73.10%and 59.87%,respectively.Shoot and root dry weights of C.officinalis significantly increased by 172.31%and 80.96%under PC and 311.61%and 145.43%under PBC,respectively as compared with P(P<0.05).Total chlorophyll contents in leaves of C.officinalis under P,PC and PBC significantly increased by 77.36%,125.50%and 79.80%,respectively(P<0.05)as compared with PB.Physical-chemical characteristics and enzymatic activity of soil in different treatments were also assessed.The highest total N,total P,available N,available P and SOM(soil organic matter)occurred in PC,followed by PBC(P<0.05).C.officinalis rhizospheric soil dehydrogenase(DHA)and polyphenol oxidase(PPO)activities in PB were lower than those of other treatments(P<0.05).The values of ACE(abundance-based coverage estimators)and Chao 1 indices for rhizospheric bacteria were the highest under PC followed by PBC,P,PB and CK(P<0.05).However,the Shannon index for bacteria was the highest under PC and PBC,followed by P,PB and CK(P<0.05).In terms of soil microbial community composition,Proteiniphilum,Immundisolibacteraceae and Solimonadaceae were relatively more abundant under PC and PBC.Relative abundances of Pseudallescheria,Ochroconis,Fusarium,Sarocladium,Podospora,Apodus,Pyrenochaetopsis and Schizothecium under PC and PBC were higher,while relative abundances of Gliomastix,Aspergillus and Alternaria were lower under PC and PBC.As per the nonmetric multidimensional scaling(NMDS)analysis,application of organic compost significantly promoted soil N and P contents,shoot length,root vitality,chlorophyll ratio,total chlorophyll,abundance and diversity of rhizospheric soil microbial community in C.officinalis.A high p H value and lower soil N and P contents induced by biochar,altered C.officinalis rhizospheric soil microbial community composition,which might have restrained its phytoremediation efficiency.The results suggest that organic compost-assisted C.officinalis phytoremediation for crude-oil contaminated soil was highly effective in the Loess Plateau,China. | WANG Jincheng JING Mingbo ZHANG Wei ZHANG Gaosen ZHANG Binglin LIU Guangxiu CHEN Tuo ZHAO Zhiguang | 2021 | Journal of Arid Land2021，13，6： | 4 |
| 7 | Classifying wakes produced by self-propelled fish-like swimmers using neural networks显示文摘We consider the classification of wake structures produced by self-propelled fish-like swimmers based on local measurements of flow variables.This problem is inspired by the extraordinary capability of animal swimmers in perceiving their hydrodynamic environments under dark condition.We train different neural networks to classify wake structures by using the streamwise velocity component,the crosswise velocity component,the vorticity and the combination of three flow variables,respectively.It is found that the neural networks trained using the two velocity components perform well in identifying the wake types,whereas the neural network trained using the vorticity suffers from a high rate of misclassification.When the neural network is trained using the combination of all three flow variables,a remarkably high accuracy in wake classification can be achieved.The results of this study can be helpful to the design of flow sensory systems in robotic underwater vehicles. | Binglin Li Xiang Zhang Xing Zhang | 2020 | Theoretical & Applied Mechanics Letters2020，10，3： | 4 |
| 8 | Effects of mepiquat chloride on yield and main properties of cottonseed under different plant densities显示文摘Background:Cottonseed oil and protein content as well as germination traits are major indicators of seed quality.However,the responses of these indicators to plant density and mepiquat chloride(MC)are still uncertain.To investigate plant density and MC effects on cotto nseed yield and main quality parameters,we con ducted a twoyear field experiment including four plant densities(1.35,2.55,3.75 and 4.95 plants·m^-2)and two closes of MC(0 and 135g·hm^-2)in Dafeng,Jiangsu Province,in 2013 and 2014.Results:The application of MC reduced plant height,fruit branch length and fruiting branch number under different plant densities,resulting in a lower and more compact plant canopy.Cottonseed yield showed a nonlinear increase as plant density increasing and achieved the highest value at 3.75 plants·m^-2,regardless of MC application.No significant interactio ns were found between plant density and MC for cotton seed yield and quality parameters.The 100-seed weight,cottonseed oil content and vigor index significantly decreased as plant density increased,while these parameters significantly increased with MC applying under different plant densities.Seed vigor index was positively correlated with 100-seed weight and seed oil con tent across different plant densities and MC treatments.Conclusions:Thus,application of MC could realize a win-win situation between cottonseed yield and main quality parameters under various densities;and plant density of 3.75 plants·m^-2 combined with 135 g·hm^-2 of MC applying is optimal for high cottonseed yield and quality in this cotton production area. | ZHAO Wenqing YAN Qiang YANG Hongkun YANG Xiaoni WANG Leran CHEN Binglin MENG Yali ZHOU Zhiguo | 2019 | Journal of Cotton Research2019，2，2： | 3 |
| 9 | Optimization Algorithms for Predictive Control Approach to Networked Bilinear Systems显示文摘 | Binglin Wang Yu Kang Jiahu Qin Yanmei Li | 2017 | 自动化学报2017，43，7： | 3 |
| 10 | Accelerated design and characterization of non-uniform cellular materials via a machine-learning based framework显示文摘Cellular materials,widely found in engineered and nature systems,are highly dependent on their geometric arrangement.A nonuniform arrangement could lead to a significant variation of mechanical properties while bringing challenges in material design.Here,this proof-of-concept study demonstrates a machine-learning based framework with the capability of accelerated characterization and pattern generation.Results showed that the proposed framework is capable of predicting the mechanical response curve of any given geometric pattern within the design domain under appropriate neural network architecture and parameters.Additionally,the framework is capable of generating matching geometric patterns for a targeted response through a databank constructed from our machine learning model.The accuracy of the predictions was verified with finite element simulations and the sources of errors were identified.Overall,our machine-learning based framework can boost the design efficiency of cellular materials at unit level,and open new avenues for the programmability of function at system level. | Chunping Ma Zhiwei Zhang Benjamin Luce Simon Pusateri Binglin Xie Mohammad H.Rafiei Nan Hu | 2020 | npj Computational Materials2020，，1： | 3 |
| 11 | Blue-Green Electroluminescence of Free-Standing Diamond Thin Films显示文摘Blue-green electroluminescence has been observed in free-standing diamond films which were deposited by microwave plasma assisted CVD on silicon substrates.The electroluminescence device is driven by a 60 Hz power supply.The threshold voltage was about 112 V peak-to-peak.The electroluminescence spectrum at room temperature,showed a blue-green band with the peak centered at 485nm suggesting band A type emission.Electroluminescence was also observed at 77K. | ZHANG Binglin SHEN Shupo WANG Jian’en HE Jintian Howard R.Shanks Moeljanto W.Leksono Robert Girvan | 1994 | Chinese Physics Letters1994，11，4： | 3 |
| 12 | THE PREPARATION OF CROSSLINKED N,O-CARBOXYMETHYL CHITOSAN RESINSAND THEIR ADSORPTION PROPERTIESFOR TRIGLYCERIDE IN SERUM显示文摘Crosslinked N, O-carboxymethyl chitosan resins which can selectively adsorb triglyceride, were prepared by the reaction of N, O- carboxymethyl chitosan with glutaraldehyde solution. Adsorption experiments were performed by adding the resins to the serum. The results showed that this type of adsorbent could cut down the concentration of triglyceride in serum by 56. 4% (3. 35mg/gresin ) at most, while concentration of the total protein (TP ) decreased only by 10. 9% at least, so this novel adsorbent can be used to cure hypertriglyceridemia by hemoperfusion in the future. | Yu Yihau He Binglin(Institute of Polymer Chemistry, The State Key Laboratory of Functional Polymer Materials for Adsorption and Separation, Nankai University, Tianjin 300071, P. R. China)Gu Hanqing(Tianjin Institute of Urological Surgery) | 1997 | Chinese Journal of Reactive Polymers1997，6，1： | 2 |
| 13 | Evolution Rules and Spatial Features of Traditional Settlements in Fukou Xing Region显示文摘As one of the eight Taihang passes,Fukou Xing is located in the south of the Taihang Mountains and has been an important passage for Shanxi and Hebei in history.Taking traditional settlements in Fukou Xing Region as research object,using the Advanced Spaceborne Thermal Emission and Reflection Radiometer(ASTER) Global Digital Elevation Model(GDEM)(remote sensing measurement of elevation data) and GIS platform,this paper made a quantitative study on traditional settlement space in mountain environment of this region,and studied space parameters including elevation,terrain,aspect,and boundary,observed and summarized the spatial features.In addition,based on the local chronicles of Ming and Qing dynasties,it mutually verified the quantitative conclusions and qualitative cognition,analyzed the evolution rules of traditional settlements in Fukou Xing region,and finally obtained new understandings of spatial features of traditional settlements in Fukou Xing region. | YUAN Lin LIU Gongxue XUE Binglin | 2018 | Journal of Landscape Research2018，10，5： | 2 |
| 14 | Effect of the organic additions on crystal growth behavior of ZrO 2 nanocrystals prepared via sol–gel process显示文摘 | Wenzhi Huang Jili Yang Xiangsheng Meng Yongliang Cheng Chunjie Wang Binglin Zou Zuhair Khan Zhen Wang Xueqiang Cao | 2011 | Chemical Engineering Journal2011，，3： | 2 |
| 15 | SWELLING AND WETTABILITY OF LIGHT-CURED METHACRYLATE-BASED DENTAL RESINS PREPARED FROM CHOLIC ACID显示文摘2′-methacryloxy-3α, 7α, 12α - trimethacryloyl cholic acid ethyl ester (CAGE4MA) has been prepared from cholic acid. Photo-polymeric resins were prepared from CAGE4MA. 2,2-bis4-(2-hydroxy-3-methacrylyloxypropoxy)phenylpropane (bis-GMA) was used for comparison, triethyleneglycol dimethacrylate (TEGDMA) was used as diluent. The polymerization was initiated by camphoroquinone (CQ)N, N-dimethylaminoethyl methacrylate (DMAEMA) system. The conversion of CAGE4MA was 39% when the reaction time is 60s, which is lower than bis-GMA and TEGDMA. The swelling value of CAGE4MA resin was 0.41% in distilled water, which is much lower than those of bis-GMA resin (2.04%) and TEGDMA resin (4.77%) under the same conditions. Copolymers from CAGE4MA and TEGDMA have been prepared. With the increase of TEGDMA in mixture, the degree of conversion of CAGE4MA and swelling value increased. The swelling values of photocured resins in 0.1molL HCl were also measured. | HU Xiangzheng ZHANG Xu WANG Zheng HE Binglin | 2005 | Chinese Journal of Reactive Polymers2005，14，1： | 2 |
| 16 | THE SYNTHESIS AND CHARACTERIZATION OFPOLY-L-LACTIDE USED FOR THE INTERNALFIXATION OF BONE FRACTURE显示文摘A high Mw of PLLA was synthesized, it intended to be used in the manufacture ofbioabsorbable screw applied in bone fracture internal fixation. The optical reactionconditions have been discussed. | Zhang Zhengpu Lu ge Wu Zhizhong He Binglin(State key Laboratory of Functional Polymer Materials for Adsorption and Separation,Institute of Polymer Chemistry, Nankai University,Tianjin 300071 ) | 1998 | Chinese Journal of Reactive Polymers1998，7，2： | 2 |
| 17 | The conceptual alternative design for ITER magnet gravity support using clamp bolt 显示文摘 | Hou Binglin Li Pengyuan Remy Gallix | 2010 | Fusion Engineering and Design2010，85，： | 1 |
| 18 | Investigations on the adsorbents for uremic middle molecular toxins (II)显示文摘 | Hong Wang Zhi Yuan Xiaohang Liu Xinlian Teng Binglin He | 2001 | Science in China Series B: Chemistry2001，，5： | 1 |
| 19 | Thermal cycling behavior and failure mechanism of La 2 (Zr 0.7 Ce 0.3 ) 2 O 7 /Eu 3+ -doped 8YSZ thermal barrier coating prepared by atmospheric plasma spraying显示文摘 | Sumei Zhao Lijian Gu Yu Zhao Wenzhi Huang Ling Zhu Xizhi Fan Binglin Zou Xueqiang Cao | 2013 | Journal of Alloys and Compounds2013，，： | 1 |
| 20 | 显示文摘 | Fan Zhiqin Zhang Binglin | 2003 | Chin Phys Lett2003，20，11： | 1 |
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