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| 1 | HDAC inhibitors improve CRISPR-mediated HDR editing efficiency in iPSCs显示文摘Genome-edited human induced pluripotent stem cells(iPSCs)hold great promise for therapeutic applications.However,low editing efficiency has hampered the applications of CRISPR-Cas9 technology in creating knockout and homology-directed repair(HDR)-edited iPSC lines,particularly for silent genes.This is partially due to chromatin compaction,inevitably limiting Cas9 access to the target DNA.Among the six HDAC inhibitors we examined,vorinostat,or suberoylanilide hydroxamic acid(SAHA),led to the highest HDR efficiency at both open and closed loci,with acceptable toxicity.HDAC inhibitors equally increased non-homologous end joining(NHEJ)editing efficiencies(~50%)at both open and closed loci,due to the considerable HDAC inhibitor-mediated increase in Cas9 and sgRNA expression.However,we observed more substantial HDR efficiency improvement at closed loci relative to open chromatin(2.8 vs.1.7-fold change).These studies provide a new strategy for HDRediting of silent genes in iPSCs. | Jian-Ping Zhang Zhi-Xue Yang Feng Zhang Ya-Wen Fu Xin-Yue Dai Wei Wen Beldon Zhang Hannah Choi Wanqiu Chen Meredith Brown David Baylink Lei Zhang Hongyu Qiu Charles Wang Tao Cheng Xiao-Bing Zhang | 2021 | Science China(Life Sciences)2021，64，9： | 3 |
| 2 | Rapid and efficient reprogramming of human fetal and adult blood CD34~ cells into mesenchymal stem cells with a single factor显示文摘进体的干细胞的皮房间的直接变换在再生药打开了新治疗学的可能性。这里,我们证明人的导致的间充质的干细胞(iMSCs ) 能高效地从绳索血(CB ) 被产生 - 或由有一个单个因素的直接 reprogramming 的成年的外部血(PB )-CD34+ 房间, OCT4。面对一个 GSK3 禁止者, 16% OCT4-transduced CD34 + 房间在 2 个星期以内被变换成 iMSCs。有效直接 reprogramming 与两 episomal 被完成调停向量的短暂 OCT4 表示和 lentiviral 调停向量的 OCT4 transduction。iMSCs 快车 MSC 标记,类似于骨头髓(BM ) 在形态学的 -MSCs,并且在 vitro multilineage 区别能力拥有,还与 BM-MSCs 相比有一个更大的 proliferative 能力。类似于 BM-MSCs,植入的 iMSCs 形式骨头和结缔组织,并且是在老鼠的 non-tumorigenic。然而, BM-MSCs 不而 iMSCs 确实形成肌肉纤维,显示 iMSCs 的一个潜在的功能的优点。另外,我们观察到 OCT4 表示的高水平为起始的 reprogramming 和最佳的 iMSC 自强被要求,当 OCT4 表示的减小为 multilineage 区别被要求时。我们的方法将贡献病人特定的 iMSCs 的产生,它能在再生药有应用。这发现可以也为血房间的直接变换便于策略的发展进临床的重要性的房间的另外的类型。 | Xianmei Meng Rui-Jun Su David J Baylink Amanda Neises Jason B Kiroyan Wayne Yuk-Wai Lee Kimberly J Payne Daila S Gridley Jun Wang K-H William Lau Gang Li Xiao-Bing Zhang | 2013 | Cell Research2013，23，5： | 2 |
| 3 | Fluid flow shear stress stimulates human osteoblast proliferation and differentiation through multiple interacting and competing signal transduction pathways显示文摘 | Kapur S Baylink DJ Lau KHW | 2003 | Bone2003，32，： | 1 |
| 4 | Characterization of cells isolated and cultured from human bone显示文摘 | Wergedal JE Baylink DJ | 1984 | Proc Soc Exp Biol Med1984，176，1： | 1 |
| 5 | Fluid flow shear stress stimulates human osteoblast proliferation and differentiation through multiple interacting and competing signal transduction pathways 显示文摘 | Kapur S Baylink DJ Lau KH | 2003 | Bone2003，32，3： | 1 |
| 6 | Fluid flow shear stress stimulates human osteoblast proliferation and differentiation through multiple interacting and competing signal transduction pathways 显示文摘 | Kapur S Baylink DJ Lau KH | 2003 | Bone2003，32，3： | 1 |
| 7 | Growth factorsfor bone growth and repair:IGF,TGF-β and BMP显示文摘 | Linkham TA Mohan S Baylink DJ | 1996 | Bone1996，，： | 1 |
| 8 | Fliud flow shear stress stimulatease human osteoblast proliferation and differentiation through multiple interacting and competing signal transduction pmhways显示文摘 | Kapur S Baylink DJ Lau KH | 2003 | Bone2003，32，3： | 1 |
| 9 | Osteoblastic tartrate-resistant acid phosphatase: its potential role in the molecular mechanism of osteo genic action of fluoride显示文摘 | Lau KHW Baylink DJ | 2003 | Journal of Bone and Mineral Research2003，18，10： | 1 |
| 10 | Differential effects of two protein tyrosine kinase inhibitors, tyrphostin and genistein,on human bone cell proliferation as compared with differentiation显示文摘 | Chen K Baylink DJ | 1998 | Calcif Tissue Int1998，63，3： | 1 |
| 11 | Phenytoin at micromolar concentration is an osteogenic agent for human-mandible-derived bone cells in vitro显示文摘 | Nakade O Baylink DJ Lau KHW | 1995 | J Dent Res1995，74，： | 1 |
| 12 | Fluoride directly stimulates proliferation and alkaline phosphatase activity of boneforming cells显示文摘 | FARLEY JR WERGEDAL JE BAYLINK DJ | 1983 | Science1983，222，： | 1 |
| 13 | Fluid flow shear stress stimulates human osteoblast proliferation and differentiation through multiple interacting and competing signal transduction pathways 显示文摘 | Kapur S Baylink D J Lau KH | 2003 | Bone2003，32，3： | 1 |
| 14 | Molecular mechanism of action of fluoride on bone cells 显示文摘 | Lau KH Baylink DJ | 1998 | J Bone Miner Res1998，13，11： | 1 |
| 15 | Inhibition of bone formation during space flight显示文摘 | Morey ER Baylink DJ | 1978 | Science1978，201，4361： | 1 |
| 16 | A time course of bone response to jump exercise in C57BL/6J mice显示文摘 | Yoshihisa Umemura David J. Baylink Jon E. Wergedal Subburaman Mohan Apurva K. Srivastava | 2002 | Journal of Bone and Mineral Metabolism2002，，4： | 1 |
| 17 | The diagnosis and management of osteoporosis显示文摘 | Baylink DJ | 2000 | Z Rheumatol2000，59，1： | 1 |
| 18 | Fluid flow shear stress stimulates human osteoblast proliferation and differentiation through multiple interacting and competing signal transduction pathways显示文摘 | Kapur S Baylink DJ Lau K H | 2003 | Bone2003，32，3： | 1 |
| 19 | Skeletal alkaline phosphatase ac- tivity in serum显示文摘 | Farley J Baylink D J | 1995 | Clinical chemistry1995，41，11： | 1 |
| 20 | Evidence that IGF-binding protein-5 functions as a growth factor显示文摘 | Miyakoshi N Richman C Kasukawa Y Linkhart TA Baylink DJ Mohan S | | 0，，： | 1 |
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