|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | HDAC inhibitors improve CRISPR-mediated HDR editing efficiency in iPSCs显示文摘Genome-edited human induced pluripotent stem cells(iPSCs)hold great promise for therapeutic applications.However,low editing efficiency has hampered the applications of CRISPR-Cas9 technology in creating knockout and homology-directed repair(HDR)-edited iPSC lines,particularly for silent genes.This is partially due to chromatin compaction,inevitably limiting Cas9 access to the target DNA.Among the six HDAC inhibitors we examined,vorinostat,or suberoylanilide hydroxamic acid(SAHA),led to the highest HDR efficiency at both open and closed loci,with acceptable toxicity.HDAC inhibitors equally increased non-homologous end joining(NHEJ)editing efficiencies(~50%)at both open and closed loci,due to the considerable HDAC inhibitor-mediated increase in Cas9 and sgRNA expression.However,we observed more substantial HDR efficiency improvement at closed loci relative to open chromatin(2.8 vs.1.7-fold change).These studies provide a new strategy for HDRediting of silent genes in iPSCs. | Jian-Ping Zhang Zhi-Xue Yang Feng Zhang Ya-Wen Fu Xin-Yue Dai Wei Wen Beldon Zhang Hannah Choi Wanqiu Chen Meredith Brown David Baylink Lei Zhang Hongyu Qiu Charles Wang Tao Cheng Xiao-Bing Zhang | 2021 | Science China(Life Sciences)2021，64，9： | 3 |
| 2 | Rapid and efficient reprogramming of human fetal and adult blood CD34~ cells into mesenchymal stem cells with a single factor显示文摘进体的干细胞的皮房间的直接变换在再生药打开了新治疗学的可能性。这里,我们证明人的导致的间充质的干细胞(iMSCs ) 能高效地从绳索血(CB ) 被产生 - 或由有一个单个因素的直接 reprogramming 的成年的外部血(PB )-CD34+ 房间, OCT4。面对一个 GSK3 禁止者, 16% OCT4-transduced CD34 + 房间在 2 个星期以内被变换成 iMSCs。有效直接 reprogramming 与两 episomal 被完成调停向量的短暂 OCT4 表示和 lentiviral 调停向量的 OCT4 transduction。iMSCs 快车 MSC 标记,类似于骨头髓(BM ) 在形态学的 -MSCs,并且在 vitro multilineage 区别能力拥有,还与 BM-MSCs 相比有一个更大的 proliferative 能力。类似于 BM-MSCs,植入的 iMSCs 形式骨头和结缔组织,并且是在老鼠的 non-tumorigenic。然而, BM-MSCs 不而 iMSCs 确实形成肌肉纤维,显示 iMSCs 的一个潜在的功能的优点。另外,我们观察到 OCT4 表示的高水平为起始的 reprogramming 和最佳的 iMSC 自强被要求,当 OCT4 表示的减小为 multilineage 区别被要求时。我们的方法将贡献病人特定的 iMSCs 的产生,它能在再生药有应用。这发现可以也为血房间的直接变换便于策略的发展进临床的重要性的房间的另外的类型。 | Xianmei Meng Rui-Jun Su David J Baylink Amanda Neises Jason B Kiroyan Wayne Yuk-Wai Lee Kimberly J Payne Daila S Gridley Jun Wang K-H William Lau Gang Li Xiao-Bing Zhang | 2013 | Cell Research2013，23，5： | 2 |
| 3 | A time course of bone response to jump exercise in C57BL/6J mice显示文摘 | Yoshihisa Umemura David J. Baylink Jon E. Wergedal Subburaman Mohan Apurva K. Srivastava | 2002 | Journal of Bone and Mineral Metabolism2002，，4： | 1 |
| 4 | Effects of calcitonin on bone quality and osteoblastic function显示文摘 | Stanley Wallach John R. Farley David J. Baylink Leona Brenner-Gati | 1993 | Calcified Tissue International1993，，5： | 1 |
| 5 | Evaluation of the usefulness of serum phosphatases and osteocalcin as serum markers in a calcium depletion-repletion rat model显示文摘 | Hiromichi Tanimoto K. -H. William Lau Satoru K. Nishimoto Jon E. Wergedal David J. Baylink | 1991 | Calcified Tissue International1991，，2： | 1 |
| 6 | Osteocyte-derived insulin-like growth factor I is essential for determining bone mechanosensitivity显示文摘 | K.-H. William Lau David J. Baylink Xiao-Dong Zhou Denise Rodriguez Lynda F. Bonewald Zihui Li Davide Ruffoni Ralph Müller Chandrasekhar Kesavan Matilda H.-C. Sheng | 2013 | AJP: Endocrinology and Metabolism2013，，2： | 1 |
| 7 | A time course of bone response to jump exercise in C57BL/6J mice显示文摘 | Yoshihisa Umemura David J. Baylink Jon E. Wergedal Subburaman Mohan Apurva K. Srivastava | 2002 | Journal of Bone and Mineral Metabolism2002，，4： | 1 |
| 8 | Insulin-like growth binding proteins in serum and other biological fluids:regulation and function显示文摘 | Sujatha R David J Baylink | 1997 | Endocrine1997，18，： | 1 |
| 9 | Effects of calcitonin on bone quality and osteoblastic function显示文摘 | Stanley Wallach John R. Farley David J. Baylink Leona Brenner-Gati | 1993 | Calcified Tissue International1993，，5： | 1 |
| 10 | Synthesis of Magnetic Nanoparticles for Application of Retroviral Vectors Mediated Gene Therapy显示文摘 Successful gene therapy depends on accurate delivery of therapeutic genes to target sites. In this report, we used magnetic nanopartieles to achieve this goal by developing magnetic Moloney leukemia virus-based (MRV) vectors. The vectors are combined by magnetic nanoparticles with the MRV viral vectors and can be guided to a specific site by an external magnetic filed.…… | Huan-Chiu Ku Ming-Fong Tai K.-H. William Lau David Baylink Shin-Tai Chen | 2004 | 过程工程学报2004，4，z1： | 1 |
| 11 | Effect of aging on stem cells显示文摘Pluripotent stem cells have the remarkable self-renewal ability and are capable of differentiating into multiple diverse cells. There is increasing evidence that the aging process can have adverse effects on stem cells. As stem cells age, their renewal ability deteriorates and their ability to differentiate into the various cell types is altered. Accordingly, it is suggested aging-induced deterioration of stem cell functions may play a key role in the pathophysiology of the various aging-associated disorders. Understanding the role of the aging process in deterioration of stem cell function is crucial, not only in understanding the pathophysiology of agingassociated disorders, but also in future development of novel effective stem cell-based therapies to treat agingassociated diseases. This review article first focuses on the basis of the various aging disease-related stem cell dysfunction. It then addresses the several concepts on the potential mechanism that causes aging-related stem cell dysfunction. It also briefly discusses the current potential therapies under development for aging-associated stem cell defects. | Abu Shufian Ishtiaq Ahmed Matilda HC Sheng Samiksha Wasnik David J Baylink Kin-Hing William Lau | 2017 | World Journal of Experimental Medicine2017，7，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员