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| 1 | Relevance of α-defensins(HNP1-3) and defensin β-1 in diabetes显示文摘AIM: To investigate the genetic background of human defensin expression in type 1 and 2 diabetes.METHODS: Associations between DEFA1/DEFA3 gene copy number polymorphism and diabetes as well as between the promoter polymorphisms of DEFB1 and diabetes were studied. The copy number variation of the DEFA1/DEFA3 genes was determined in 257 diabetic patients(117 patients with type 1 and 140 with type 2 diabetes). The control group consisted of 221 age- and gender-matched healthy blood donors. The cumulative copy numbers of the DEFA1/DEFA3 genes were detected by using quantitative PCR analysis. To evaluate the HNP 1-3(human neutrophil peptide 1-3 or α-defensin) levels in the circulation, plasma HNP 1-3 concentrations were measured by ELISA. The expression of DEFA1/A3 in peripheral leukocytes of the diabetic patients was measured by quantitative RT PCR analysis. Three SNPs of the human DEFB1(human defensin β-1) gene: DEFB1 G-20A(rs11362), DEFB1 C-44G(rs1800972) and DEFB1 G-52A(rs1799946) were genotyped by Custom TaqMan? Real Time PCR assay.RESULTS: Significant differences were observed in HNP1-3 levels between the healthy subjects and both groups of diabetic patients. The mean ± SE was 28.78 ± 4.2 ng/mL in type 1 diabetes, and 29.82 ± 5.36 ng/mL in type 2 diabetes, vs 11.94 ± 2.96 ng/mL in controls; P < 0.01 respectively. There was no significant difference between patients with type 1 and type 2 diabetes in the high plasma concentrations of HNP1-3. The highest concentrations of α-defensin were found in diabetic patients with nephropathy(49.4 ± 4.8 ng/mL), neuropathy(38.7 ± 4.8 ng/mL) or cardiovascular complications(45.6 ± 1.45 ng/L). There was no significant difference in the cumulative copy numbers of DEFA1/DEFA3 genes between controls and patients, or between patients with the two types of diabetes. Comparisons of HNP 1-3 plasma level and DEFA1/A3 copy number of the same patient did not reveal significant relationship between defensin-α levels and the gene copy numbers(r2 = 0.01). Similarly, no positive correlation was observed between the copy numbers and the mRNA expression levels of DEFA1/A3. Regarding the C-44G polymorphism of DEFB1, the GG 'protective' genotype was much less frequent(1%-2%) among both groups of patients than among controls(9%).CONCLUSION: Elevated HNP1-3 levels in diabetes are independent of DEFA1/DEFA3 copy numbers, but GG genotype of C-44G SNP in DEFB1 gene may result in decreased defensin β-1 production. | Balázs Csaba Németh Tamás Várkonyi Ferenc Somogyvári Csaba Lengyel Katalin Fehértemplomi Szabolcs Nyiraty Péter Kempler Yvette Mándi | 2014 | World Journal of Gastroenterology2014，20，27： | 4 |
| 2 | A role for the Hsp90 molecular chaperone family in antigen presentation to T lymphocytes via major histocompatibility complex class II molecules显示文摘 | Rajagopal D Bal V Mayor S George A Rath S | 2006 | Eur J Immunol2006，36，4： | 1 |
| 3 | Psychological contract breach and job attitudes:A meta-analysis of age as a moderator 显示文摘 | Bal P M De Lange A H Jansen P G W Der Velde M E G V | 2008 | Journal of Vocational Behavior2008，72，1： | 1 |
| 4 | Intravitalconfocal and two-photon imaging of dual-color cellsand extracellular matrix mimics 显示文摘 | Bal U Andresen V Baggett B et al | 2013 | MicroscMicroanal2013，19，1： | 1 |
| 5 | Lipid oxidation and cholesterol oxidation in mutton during cooking and storage 显示文摘 | Bal Kowale N Kesava Rao V Pedda Babu N | 1996 | Meat Science1996，43，2： | 1 |
| 6 | Stress proteins may provide a link between the immune response to infection and autoimmunity显示文摘 | Lamb JR Bal V Mendez Samperio A | 1989 | Int Immunol1989，1，： | 1 |
| 7 | Combined treatment with verapamil,a calcium channel blocker,and B428,a synthetic uPA inhibitor,impairs the metastatic ability of a murine mammary carcinoma显示文摘 | Todaro LB Ladeda V Bal de Kier Joffe E | 2003 | Oncol Rep2003，10，3： | 1 |
| 8 | Comments on the definition of the Q2 parameter for QSAR validation显示文摘 | Consonni V Bal abio D Todeschini R | | 0，，： | 1 |
| 9 | Reduced antioxidant capaci- ties in platelets from patients with autoimmune thromboeytopenia pur- pura (ITP) 显示文摘 | Kamhieh-Milz J Bal G Sterzer V | 2012 | Platelets2012，23，3： | 1 |
| 10 | Process technology for production of soluble tea powder显示文摘 | V R Sinija H N Mishra S Bal | 2007 | Journal of Food Engineering2007，，82： | 1 |
| 11 | Aluminium-induced oxi- dative DNA damage recognition and cell-cycle dis- ruption in different regions of rat brain 显示文摘 | Kumar V Bal A Gill KD | 2009 | Toxi- cology2009，264，3： | 1 |
| 12 | Can the C-14 Urea Breath Test Replace Follow-up Endoscopic Biopsies in Patients Treated for Helicobacter Pylori Infection? 显示文摘 | Ahuja V Bal CS Sharma MP | 1998 | Clin Nucl Med1998，23，12： | 1 |
| 13 | Process technology for production of soluble tea powder显示文摘 | Sinija V R Mishra H N Bal S | 2007 | Journal of Food Engineering2007，82，3： | 1 |
| 14 | Production of the postharvest biocontrol agent Bacillus subtilis CPA-8 using low cost commercial products and by-products显示文摘 | Y(A)NEZ-MENDIZ(A)BAL V VI(N)AS I USALL J | | 0，，： | 1 |
| 15 | Fluorescent microscopy and Ziehl-Neelsen staining of bronchoalveolar lavage, bronchial washings, bronchoscopic brushing and post bronchoscopic sputum along with cytological examination in cases of suspected tuberculosis显示文摘 | Bodal V K Bal M S Bhagat S | 2015 | Indian J Pathol Microbiol2015，58，4： | 1 |
| 16 | Methamphetamine in hair and interpretation of forensic findings in a fatal case显示文摘 | BERNKOVK HABRDOVV BALKOVM | 2005 | Forensic Sci Int2005，153，1： | 1 |
| 17 | The Mining Industry in the European Union:Analysis of Inter-Industry Linkages Using Input-Output Analysis显示文摘 | San Cristóbal J R Biezma M V | 2006 | Resources Policy2006，，1： | 1 |
| 18 | Integrating alkaline extraction of proteins with enzymatic hydrolysis of cellulose from wet distiller's grains and solubles显示文摘 | Bals B Balan V Dale B | 2009 | Bioresource Technology2009，100，23： | 1 |
| 19 | Synthesis and biological properties of triiodothyrocarboxylic acids 显示文摘 | BAL'ON Y G KORPACHEV V V SIMUROV O V | 2003 | Farmatsevtichnii Zhurnal (Kiev)2003，6，： | 1 |
| 20 | Interactions between soil development, vegetation and soil fauna during spontaneous succession in post mining sites 显示文摘 | Frouz J Prach K Pi?l V Háněl L Stary J Tajovsky K Materna J Balík V Kal?ík J ?ehounková K | 2008 | Eur J Soil Biol2008，44，1： | 1 |
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