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| 1 | Aging related methylation influences the gene expression of key control genes in colorectal cancer and adenoma显示文摘AIM To analyze colorectal carcinogenesis and age-related DNA methylation alterations of gene sequences associated with epigenetic clock CpG sites. METHODS In silico DNA methylation analysis of 353 epigenetic clock Cp G sites published by Steve Horvath was performed using methylation array data for a set of 123 colonic tissue samples [64 colorectal cancer(CRC), 42 adenoma, 17 normal; GEO accession number: GSE48684]. Among the differentially methylated agerelated genes, secreted frizzled related protein 1(SFRP1) promoter methylation was further investigated in colonic tissue from 8 healthy adults, 19 normal children, 20 adenoma and 8 CRC patients using bisulfite-specific PCR followed by methylation-specific high resolution melting(MS-HRM) analysis. m RNA expression of age-related 'epigenetic clock' genes was studied using Affymetrix HGU133 Plus2.0 whole transcriptome data of 153 colonic biopsy samples(49 healthy adult, 49 adenoma, 49 CRC, 6 healthy children)(GEO accession numbers: GSE37364, GSE10714, GSE4183, GSE37267). Whole promoter methylation analysis of genes showing inverse DNA methylationgene expression data was performed on 30 colonic samples using methyl capture sequencing.RESULTS Fifty-seven age-related Cp G sites including hypermethylated PPP1R16 B, SFRP1, SYNE1 and hypomethylated MGP, PIPOX were differentially methylated between CRC and normal tissues(P < 0.05, ?β≥ 10%). In the adenoma vs normal comparison, 70 CpG sites differed significantly, including hypermethylated DKK3, SDC2, SFRP1, SYNE1 and hypomethylated CEMIP, SPATA18(P < 0.05, ?β≥ 10%). In MS-HRM analysis, the SFRP1 promoter region was significantly hypermethylated in CRC(55.0% ± 8.4 %) and adenoma tissue samples(49.9% ± 18.1%) compared to normal adult(5.2% ± 2.7%) and young(2.2% ± 0.7%) colonic tissue(P < 0.0001). DNA methylation of SFRP1 promoter was slightly, but significantly increased in healthy adults compared to normal young samples(P < 0.02). This correlated with significantly increased SFRP1 m RNA levels in children compared to normal adult samples(P < 0.05). In CRC tissue the mR NA expression of 117 agerelated genes were changed, while in adenoma samples 102 genes showed differential expression compared with normal colonic tissue(P < 0.05, logF C > 0.5). The change of expression for several genes including SYNE1, CLEC3 B, LTBP3 and SFRP1, followed the same pattern in aging and carcinogenesis, though not for all genes(e.g., MGP). CONCLUSION Several age-related DNA methylation alterations can be observed during CRC development and progression affecting the m RNA expression of certain CRC- and adenoma-related key control genes. | Orsolya Galamb Alexandra Kalmár Barbara Kinga Barták árpád V Patai Katalin Leiszter Bálint Péterfia Barnabás Wichmann Gábor Valcz Gábor Veres Zsolt Tulassay Béla Molnár | 2016 | World Journal of Gastroenterology2016，22，47： | 7 |
| 2 | 高温环境中训练和比赛的共识性建议显示文摘高温环境中运动会引起体温调节和其它生理压力,继而可导致耐力运动能力的损害。本共识性声明的目的是提供最新的建议以使热环境中体育活动时的运动能力最优化。可用于降低热应激压力和优化运动能力的最重要干预方式是热习服,其应包括1~2周以上反复的运动—高温环境暴露。此外,运动员应在正常水合状态下开始比赛和训练,并将运动中的脱水最小化。随着商用降温系统(如降温背心)的发展,在高温环境中训练或比赛前,运动员可以采取降温策略来促进热的散发或提高蓄热能力。而且,赛事组织者应该设计大面积的遮阳区域,并提供降温和补水设施,按照最小化运动员的健康风险来安排赛事,尤其是在大众参与的赛事中及一年之中炎热天气开始之初。以最近的2008年奥运会和2014年国际足联世界杯为例,当比赛在高温环境中举行时,赛事主管机构应考虑在比赛中或比赛之间允许额外的(或更长的)恢复期以提供补水和降温的时机。 | Sébastien Racinais Juan-Manuel Alonso Aaron J.Coutts Andreas D.Flouris Olivier Girard José González -Alonso Christophe Hausswirth Ollie Jay Jason K.W.Lee Nigel Mitchell George P.Nassis Lars Nybo Babette M.Pluim Bart Roelands Michael N.Sawka Jonathan Wingo Julien D.Périard. 徐金成 高璨 赵杰修 | 2016 | 中国运动医学杂志2016，35，2： | 4 |
| 3 | Coupled package-device nxxtding for microelectromechanical systems 显示文摘 | Bart S F Zhang Su-san Rablnovich V L | 2000 | Microelectronics and Rdiability2000，40，： | 1 |
| 4 | Electric micromotor dynamics显示文摘 | Bart S F Mehregany M Tavrow L S | 1992 | IEEE Transactions on Electron Devices1992，39，3： | 1 |
| 5 | computer simulation of bony tissue response to a partial removable denture fitted to a lower jaw显示文摘 | BARTKOV S SUCHNEK J MIULKA J | 2003 | Scripta medica (BRNO)2003，76，1： | 1 |
| 6 | A genetic input selection methodology for identification of the cleaning process on a combine harvester,Part Ⅱ:selection of relevant input variables for identification of material other than grain (MOG)content in the grain bin显示文摘 | Geert C Wouter S Bart M | | 0，，： | 1 |
| 7 | Identification of the cleaning process on combine harvesters,part II:a fuzzy model for prediction of the sieve losses显示文摘 | Geert C Wouter S Bart M | | 0，，02： | 1 |
| 8 | The Decreased Apical Dominance1/Petunia Hybrida Carotenoid Cleavage Dioxygenase8 Gene Affects Branch Production and Plays a Role in Leaf Senescence,Root Growth and Flower Development显示文摘 | KIMBERLEY C S ANDREW J S BART J J | 2005 | The Plant Cell2005，17，： | 1 |
| 9 | Cryosurgery in aggressive,benign,and low-grade malignant bone tumours显示文摘 | RenéV Bart S Hermanvan B | 2005 | Lancet Oncol2005，6，： | 1 |
| 10 | A genetic input selection methodology for identification of the cleaning process on a combine harvester,Part I:Selection of relevant input variables for identification of the sieve losses显示文摘 | Geert C Wouter S Bart M | | 0，，02： | 1 |
| 11 | Use of an alpha- galactosidase gene as a food-grade selection marker for Streptococcus thermophilus显示文摘 | LABRIE S BART C VADEBONCOEUR C | 2005 | Journal of Dairy Science2005，88，7： | 1 |
| 12 | Fibrates downregulate apolipoprotein C-Ⅲ expression independent of induction of peroxisomal aeyl coenzyme A oxidase: a potential mechanism for the hypolipidemic action of drugs显示文摘 | BART S NGOC VD | 1995 | J Clin Invest1995，95，2： | 1 |
| 13 | Use of an alpha-galactosidase gene as a food-grade selection marker for Streptococcus thermophilus显示文摘 | Labrie S Bart C Vadeboncoeur C | 2005 | J Dairy Sci2005，88，7： | 1 |
| 14 | Assisted Reproductive Technologies Are Associated With Cardiovascular Remodeling In Utero That Persists Postnatally显示文摘 | Brenda Valenzuela-Alcaraz Fàtima Crispi Bart Bijnens Monica Cruz-Lemini Montserrat Creus Marta Sitges Joaquim Bartrons Salvadora Civico Juan Balasch Eduard Gratacós | 2013 | Circulation2013，，13： | 1 |
| 15 | Stress-indueed immune changes in the oyslcr Crassostrea gigas 显示文摘 | Lacoste A Malham S K Gélébart F | 2001 | Developmental and Comparative Immunology2001，26，： | 1 |
| 16 | Cardiorenalrescue study in acute decompensated heart failure : rationaleand design of CARRESS-HF, for the Heart Failure ClinicalResearch Network显示文摘 | BART B A GOLDSMITH S R LEE K L | 2012 | J Card Fail2012，18，3： | 1 |
| 17 | Chemisorptive catalytic oxidation process for SO2 from smelting waste gases by Fe(I)显示文摘 | Bart H J Ning P Sun P S | 1996 | Separations Technology61996，6，4： | 1 |
| 18 | Role of the PPAR in mediating the effects of fibrates and fatty acids on gene expression显示文摘 | Kristina S Bart S | 1996 | J Lipid Res1996，37，： | 1 |
| 19 | Transoral Incisionless Fundoplication: 2-year Results from the Prospective Multicenter U.S. Study显示文摘 | Bell Reginald C W Barnes William E Carter Bart J Sewell Robert W Mavrelis Peter G Ihde Glenn M Hoddinott Kevin M Fox Mark A Freeman Katherine D Gunsberger Tanja Hausmann Mark G Dargis David Gill Brian DaCosta Wilson Erik Trad Karim S | 2014 | The American Surgeon2014，，11： | 1 |
| 20 | An HIV-1 clade C DNA prime,NYVAC boost vaccine regimen induces reliable,polyfunctional,and long-lasting T cell responses显示文摘 | Harari A Bart PA St(o)hr W Tapia G Garcia M Medjitna-Rais E Burnet S Cellerai C Erlwein O Barber T Moog C Liljestrom P Wagner R Wolf H Kraehenbuhl JP Esteban M Heeney J Frachette MJ Tartaglia J McCormack S Babiker A Weber J Pantaleo G | 2008 | J Exp Med2008，205，1： | 1 |
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