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| 1 | Characterization of codon usage bias in the dUTPase gene of duck enteritis virus显示文摘A comparative analysis of the codon usage bias in the newly discovered dUTPase gene (Assigned Accession No.:DQ486149) of the duck enteritis virus (DEV) and the dUTPase gene of 32 reference herpesviruses was performed.The results indicated that the DEV dUT-Pase gene encodes a protein of 477 amino acids, which includes five conserved motifs with a 3-1-2-4-5 arrangement.The codon adap-tation index (CAI), efiective number of codons (ENC), and GC3S values indicated synonymous codon usage bias in the dUTPase gene of herpesviruses, and this synonymous bias was correlated with host evolution.The codon usage patterns of the DEV dUTPase gene were phylogenetically conserved and similar to that of the dUTPase genes of the avian alphaherpesvirus.Although codon usage in each micro-organism was difierent, there were no strain-specific difierences among them.Sixty-one codons in the predicted polypeptide, with a strong bias towards A and T at the third codon position, were used.Comparison of the codon usage in the dUTPase gene of difierent organisms revealed that there were 19 codons showing distinct codon usage difierences between the DEV and Escherichia coli dUTPase genes;16 between the DEV and yeast dUTPase genes;and 15 between the DEV and human dUTPase genes.Analysis of variance (ANOVA) showed significant difierences between the DEV and yeast dUTPase genes (r = 0.536, P < 0.01).The extent of codon usage bias in the DEV dUTPase gene was highly correlated with the gene expression level, therefore the results may provide useful information for gene classification and functional studies. | Lichan Zhao Anchun Cheng Mingshu Wang Guiping Yuan Mingsheng Cai | 2008 | Progress in Natural Science:Materials International2008，18，9： | 4 |
| 2 | Host cell apoptosis induced by infection with duck swollen head hemorrhagic disease virus显示文摘This study aimed to examine the host cell apoptosis in the tissues of Peking ducks infected with duck swollen head hemorrhagic disease virus(DSHDV).The dynamic changes associated with apoptosis occurring in the internal tissues were evaluated at different time points postinoculation(PI) by performing hematoxylin and eosin(HE) staining,followed by light microscopy,terminal deoxynucleotidyl transferase dUTP nick-end labeling(TUNEL) assay,and transmission electron microscopy(TEM).The results showed that DSHDV infection could induce apoptosis in host cells,including those of the bursa of Fabricius(BF),thymus,spleen,liver,intestinal tract,kidney,and esophagus.The apoptotic index(AI) values increased with time from 2 to 72 h PI,and the highest values were recorded at 72 h PI.Further,cell death due to classic necrosis was observed in the dying or deceased ducks after 72 h PI.In conclusion,host cell apoptosis can be induced by DSHDV and may play an important role in the pathogenesis of duck viral swollen head hemorrhagic disease(DVSHD). | Chuanfeng Li Anchun Cheng Mingshu Wang Chanjuan Shen Na Zhang Yi Zhou Dekang Zhu Qihui Luo Renyong Jia Xiaoyue Chen | 2009 | Progress in Natural Science:Materials International2009，19，1： | 3 |
| 3 | Nerve growth factor and inducible nitric oxide synthase expression in the mesencephalon and diencephalon, as well as visual-and auditory-related nervous tissues, in a macaque model of type 2 diabetes显示文摘The present study detected distribution and expression of nerve growth factor and inducible nitric oxide synthase in the mesencephalon and diencephalon, as well as visual- and auditory-related nervous tissues, in a macaque model of type 2 diabetes using immunohistochemistry. Results showed that nerve growth factor expression decreased, but inducible nitric oxide synthase expression increased, in the mesencephalon and diencephalon, as well as visual- and auditory- related nervous tissues. These results suggested that nerve growth factor and inducible nitric oxide synthase play an important role in regulating the development of diabetic visual- and auditory-related diseases. | Qihui Luo Wentao Liu Jingyao Chen Mingshu Wang Wen Zeng Zhengli Chen Anchun Cheng | 2012 | Neural Regeneration Research2012，7，4： | 2 |
| 4 | Dynamic changes of apoptosis in duck embryo fibroblasts induced by new type Gosling viral enteritis virus显示文摘The monolayer duck embryo fibroblast (DEF) cells were experimentally infected with new type Gosling viral enteritis virus (NGVEV) and the dynamic changes of apoptosis were detected at different time points after NGVEV infection by transmission electron microscopy (TEM),DNA agarose gel electrophoresis and Annexin V-FITC/PI stained fluorescence-activated cell sorter (FACS). The result shows that NGVEV can induce infected cells undergoing apoptosis and changing regularly. A series of characteristic apoptotic morphological changes including shrinkage of the cells,chromatin condensation and margination,as well as formation of apoptotic bodies,were observed by TEM. The typical ladder pattern of DNA fragmentation was demonstrated by agarose gel electrophoresis. And using flow cytometry analysis of Annexin V-FITC/PI staining,the dead,viable,apoptotic and necrotic cells could be analyzed quantitatively. | Shun Chen Anchun Cheng Mingshu Wang Xiaoyue Chen | 2008 | Progress in Natural Science:Materials International2008，18，2： | 1 |
| 5 | Electron microscopic studies of the morphogenesis of duck enteritis virus显示文摘 | Yuan Guiping Cheng Anchun Wang Mingshu et cd | 2005 | Avian Diseases2005，49，： | 1 |
| 6 | Development and e- valuation of an antigen-capture ELISA for detection of the UL24 antigen of the duck enteritis virus, based on a polyclonal antibody against the UL24 expression protein 显示文摘 | Renyong Jia Anchun Cheng | 2009 | Journal of Virological Methods2009，，161： | 1 |
| 7 | Development and evaluation of an immunoehromatographic strip test based on the recombinant UL51 protein for detecting antibody against duck enteritis virus 显示文摘 | Chanjuan Shen Anchun Cheng | 2010 | Virology Journal2010，7，： | 1 |
| 8 | Innate sensing of viruses by pattern recognition receptors in birds显示文摘 | Shun Chen Anchun Cheng Mingshu Wang | 2013 | Vet Res2013，44，1： | 1 |
| 9 | The Inlay Osteotome Sinus Augmentation Technique for Placing Short Implants Simultaneously with Reduced Crestal Bone Height. A Short‐Term Follow‐Up显示文摘 | Minhua Teng Xing Liang Quan Yuan Jing Nie Jun Ye Qian Cheng Junjiang Zhai Jian Liao Xu Sun Cai Wen Anchun Mo | 2013 | Clinical Implant Dentistry and Related Research2013，，6： | 1 |
| 10 | Development and application of a reverse transcriptase polymerase chain reaction to detect Chinese isolates of duck hepatitis virus type l显示文摘 | CHENG Anchun WANG Mingshu XIN Hongyi | 2009 | Journal of Microbiological Methods2009，76，1： | 1 |
| 11 | Construction of an Infectious Clone for Mosquito-Derived Tembusu Virus Prototypical Strain显示文摘Dear Editor,Duck Tembusu virus(DTMUV),a member of the Flavivirus genus within the Flaviviridae family,has caused huge economic losses to the poultry industry in China and even in Asia since 2010(Zhang et al.2017).The first strain of Tembusu virus(TMUV)MM_1775 was isolated from mosquitoes in 1955 in Malaysia(Platt et al.1975).DTMUV CQW1 strain(GenBank:KM233707.1)was isolated from the liver tissue of Cherry Valley ducks in southwest China in 2015(Zhu et al.2015).The positive control rCQW1 was rescued from an infectious clone that contained the full-length cDNA of CQW1.And the complete cDNA was positioned under the control of the T7 promoter elements for in vitro transcription(Chen et al.2018).The relationship between TMUV evolution and pathogenic variants has not been revealed.The fundamental reason is the lack of the in vitro operation platform for the prototypical strain genome. | Xiaoli Wang Yu He Jiaqi Guo Bowen Jiang Mingshu Wang Renyong Jia Dekang Zhu Mafeng Liu Xinxin Zhao Qiao Yang Ying Wu Shaqiu Zhang Yunya Liu Ling Zhang Yanling Yu Anchun Cheng Shun Chen | 2021 | Virologica Sinica2021，36，6： | 1 |
| 12 | Molecular cloning, characterization and tissue expression of CD4 in Chinese goose显示文摘 | Xiaoling Yan Fei Liu Shun Chen Qiurong Zhao Yulin Qi Mingshu Wang Renyong Jia Dekang Zhu Xiaoyue Chen Anchun Cheng | 2013 | Gene2013，，2： | 1 |
| 13 | Electron microscopic studies of the morphogenesis of duck enteritis virus显示文摘 | YUAN GUIPING CHENG ANCHUN WANG MINGSHU | 2005 | Avian Dis2005，49，1： | 1 |
| 14 | Intestinal mucosal immune response in ducklings following oral immunisation with an attenuated Duck enteritis virus vaccine显示文摘 | Xiaoyan Yang Xuefeng Qi Anchun Cheng Mingshu Wang Dekang Zhu Renyong Jia Xiaoyue Chen | 2009 | The Veterinary Journal2009，，2： | 1 |
| 15 | Embryotoxicity and Teratogenicity of Rhein显示文摘 | Yanchun Hu Chaorong Zeng Fei Liao Hui Tan Lei Wu Xi Liu Zhicai Zuo Junliang Deng Anchun Cheng Yahui Wei | 2014 | Journal of Health Science2014，2，4： | 0 |
| 16 | Expression of estrogen receptor alpha,nerve growth factor,interleukin-2,and androgen receptor in the cerebellum of ovariectomized rats following soybean isoflavone treatment显示文摘BACKGROUND: Studies have shown that estrogen receptor alpha (ERα), nerve growth factor(NGF), interleukin-2 (IL-2), and androgen receptor (AR) expression in the cerebellum decreaseswhen estrogen levels decrease in vivo. Soybean isoflavone, a type of non-steroid estrogen withsimilar molecular structure and function to estradiol, exhibits estrogen-like characteristics.OBJECTIVE: To investigate the effects of various doses of soybean isoflavone on expression ofERα, NGF, IL-2, and AR in the cerebellum of ovariectomized rat, and to determine whether there is adose-dependent effect.DESIGN, TIME AND SETTING: Controlled trial at the cellular and molecular level. The study wasperformed at the Experimental Animal Engineering Center, College of Veterinary Medicine, SichuanAgricultural University from July 2006 to May 2008.MATERIALS: Soybean isoflavone, comprised of daidzin, genistein and isoflavone, was provided byTaiyuan Yuantai Biochemical Industry, China. The ERα, NGF, IL-2, and AR in situ hybridization kit,rabbit anti-rat ERα, NGF, IL-2, and AR monoclonal antibodies, and SABC kit were purchased fromWuhan Boster Biological Technology, China.METHODS: A total of 50 female, Sprague Dawley rats, aged 3 months, were randomly assigned to5 groups, with 10 animals in each group. With the exception of the sham-operation group (abdominalcavity opening alone), all rats underwent bilateral ovariectomy. At 14 days after surgery, rats in thehigh-, middle-, and low-dose soybean isoflavone groups were subcutaneously injected with 1.5, 1.0,and 0.5 mg/kg soybean isoflavone, respectively, every 2 days for 6 consecutive weeks. Rats in thesham-operation and ovariectomized groups were subcutaneously injected with absolute alcohol(0.5 mL/kg).MAIN OUTCOME MEASURES: Expression levels and distribution of ERα, NGF, IL-2, and AR in thecerebellum were detected by immunohistochemistry and in situ hybridization.RESULTS: Compared with the sham-operation group, immunoreactive products and hybridizationsignals of ERα, NGF, IL-2, and AR were significantly decreased in the cerebellar cortex and nuclei ofovariectomized rats (P< 0.05 or P< 0.01), but increased following soybean isoflavone treatment. Inparticular, levels of the high-dose soybean isoflavone group were almost restored to levels of thesham-operation group (P > 0.05). The immunoreactive products were primarily located in thecytoplasm and neurites, and rarely in the cell membrane and nuclei. However, the hybridizationsignals were predominantly located in the nuclei, but rarely in the cytoplasm, cell membrane, orneurites.CONCLUSION: Soybean isofiavone upregulated ERα, NGF, IL-2, and AR protein and geneexpression in a dose-dependent manner, and played an important role in sustaining and protectingstructure and function of cerebellar neurons. Moreover, the similarity of expression patterns of thesemolecules indicated that they were mutually interactive during the regulation of soybean isoflavoneto the cerebellum. | Zhengli Chen Chunmei Zhu Qihui Luo Kangcheng Pan Anchun Cheng | 2009 | Neural Regeneration Research2009，4，12： | 0 |
| 17 | Comparative study of the pathogenicity of the mosquito origin strain and duck origin strain of Tembusu virus in ducklings and three-week-old mice显示文摘Dear Editor,Duck Tembusu virus(DTMUV)is the causative agent of a new,acute and severe infectious disease in ducks(Su et al.,2011).TMUV was first isolated from Culex tritaeniorhynchus in Malaysia in 1955(Platt et al.,1975),and this strain is regarded as a reference strain.In our previous study,an infectious clone for the mosquito-derived Tembusu virus prototypical strain MM_1775(GenBank:JX477685.2)was constructed,and the rMM_1775 virus was rescued successfully(Wang et al.,2021).And the TMUV strain rCQW1 was rescued by a reverse genetic system using the viral RNAs of CQW1(GenBank:KM233707.1)(Chen et al.,2018;Guo et al.,2020),which was isolated from ducks in 2013(Zhu et al.,2015). | Xiaoli Wang Yu He Jiaqi Guo Zhen Wu Andres Merits Mingshu Wang Renyong Jia Dekang Zhu Mafeng Liu Xinxin Zhao Qiao Yang Ying Wu Shaqiu Zhang Juan Huang Xumin Ou Qun Gao Di Sun Anchun Cheng Shun Chen | 2023 | Virologica Sinica2023，38，5： | 0 |
| 18 | Reproductive toxicity study with a novel deoxyguanosine analogue (Metacavir) in pregnant SD rats显示文摘我们的初步的研究证明 Metacavir 有潜力成为一个新 anti-HBV 代理人。在恒河猴, Metacavir 的有毒的效果的主要目标是胃肠的道,肝,血,和肾,它不与 mitochondrial 效果有关。在 Metacavir 的 intragastric 管理以后在怀孕 Sprague-Dawley 老鼠在这研究,母亲的毒性,胚胎胎儿的发展毒性和 teratogenicity 被学习(200, 100, 50, 0 mg/kg 身体重量) 在怀孕的开始的 615 天期间。更慢的重量获得从受到 200 mg/kg 剂量的 21 只老鼠在 5 被观察,以及 2 从 20 使遭到了到 100 mg/kg 剂量。与溶剂控制组相比,在 200 mg/kg 和 100 mg/kg 剂量的刻度重量获得分别地组织,在开始的 620 怀孕的天期间是显著地不同的(P P P < 0.05 ) ,当崽发生没显示出差别时。没有联系 Metacavir 的病理学的变化被观察。现在的研究在 200 mg/ 的剂量显示了那(kg 瀠瑡敩瑮 ? 捡楨癥摥猠' 咨 A 畦 ? 汣獯牵 ? 景琠敨物映獩畴慬琠慲瑣 ? 愠摮爠 ' 糖G镁?耶喩鼡疀 J? 湩漠敮瀠瑡敩瑮。 | Qihui Luo Zhengli Chen Anchun Cheng Mingshu Wang Jing Fang Xi Peng Li Tang | 2015 | Frontiers of Medicine2015，9，1： | 0 |
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