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| 1 | Matthay proteomics:current techniques and potential applications to lung disease 显示文摘 | Jan Hirsch Hansen K C Alma L | 2004 | Am J Physiol Lung Cell Mol Physiol2004，287，： | 1 |
| 2 | No association between ApoE and schizophrenia: Evidence of systematic review and updated meta-analysis显示文摘 | Thelma Beatriz González-Castro Carlos Alfonso Tovilla-Zárate Yazmín Hernández-Díaz Ana Fresán Isela E. Juárez-Rojop Jorge L. Ble-Castillo Lilia López-Narváez Alma Genis Mervyn Manuel Hernández-Alvarado | 2015 | Schizophrenia Research2015，，： | 1 |
| 3 | HP0953-hypothetical virulence factor overexpresion and localization during Helicobacter pylori infection of gastric epithelium显示文摘BACKGROUND The high prevalence and persistence of Helicobacter pylori(H. pylori) infection, as well as the diversity of pathologies related to it, suggest that the virulence factors used by this microorganism are varied. Moreover, as its proteome contains 340hypothetical proteins, it is important to investigate them to completely understand the mechanisms of its virulence and survival. We have previously reported that the hypothetical protein HP0953 is overexpressed during the first hours of adhesion to inert surfaces, under stress conditions, suggesting its role in the environmental survival of this bacterium and perhaps as a virulence factor.AIM To investigate the expression and localization of HP0953 during adhesion to an inert surface and against gastric(AGS) cells.METHODS Expression analysis was performed for HP0953 during H. pylori adhesion. HP0953 expression at 0,3, 12, 24, and 48 h was evaluated and compared using the Kruskal-Wallis equality-of-populations rank test. Recombinant protein was produced and used to obtain polyclonal antibodies for immunolocalization. Immunogold technique was performed on bacterial sections during adherence to inert surfaces and AGS cells, which was analyzed by transmission electron microscopy. HP0953 protein sequence was analyzed to predict the presence of a signal peptide and transmembrane helices, both provided by the ExPASy platform, and using the GLYCOPP platform for glycosylation sites. Different programs, via, I-TASSER, RaptorX, and HHalign-Kbest, were used to perform three-dimensional modeling.RESULTS HP0953 exhibited its maximum expression at 12 h of infection in gastric epithelium cells.Immunogold technique revealed HP0953 localization in the cytoplasm and accumulation in some peripheral areas of the bacterial body, with greater expression when it is close to AGS cells.Bioinformatics analysis revealed the presence of a signal peptide that interacts with the transmembrane region and then allows the release of the protein to the external environment. The programs also showed a similarity with the Tip-alpha protein of H. pylori. Tip-alpha is an exotoxin that penetrates cells and induces tumor necrosis factor alpha production, and HP0953 could have a similar function as posttranslational modification sites were found;modifications in turn require enzymes located in eukaryotic cells. Thus, to be functional, HP0953 may necessarily need to be translocated inside the cell where it can trigger different mechanisms producing cellular damage.CONCLUSION The location of HP0953 around infected cells, the probable posttranslational modifications, and its similarity to an exotoxin suggest that this protein is a virulence factor. | Nancy K Arteaga-Resendiz Gerardo E Rodea Rosa María Ribas-Aparicio Alma L Olivares-Cervantes Juan Arturo Castelán-Vega Joséde Jesús Olivares-Trejo Sandra Mendoza-Elizalde Edgar O López-Villegas Christian Colín Pamela Aguilar-Rodea Alfonso Reyes-López Marcela Salazar García Norma Velázquez-Guadarrama | 2022 | World Journal of Gastroenterology2022，28，29： | 1 |
| 4 | A reaction- diffusion model of ROS- induced ROS release in a mitochondrial network 显示文摘 | Zhou L Aon MA Almas T | 2010 | PLoS Comput Biol2010，6，10： | 1 |
| 5 | Volatile compo-nents of roasted Peanuts: Basic Fraction显示文摘 | B R Johnson George R Waller Alma L Burlingame | 1971 | Agric food chem1971，19，5： | 1 |
| 6 | Inactivation of Alicyclobacillus acidoterrestris vegetative ceils in model system, apple,orange and tomato juice by high hydrostatic pressures显示文摘 | Alpas H Alma L Bozoglu F | 2003 | World J Microb Biot2003，19，： | 1 |
| 7 | A reaction-diffusion model ofROS-induced ROS release in a mitochondrial network显示文摘 | Zhou L Aon MA Almas T | 2010 | PLoSComput Biol2010，6，10： | 1 |
| 8 | A reaction-diffusion model of ROS-induced ROS releases in a mitochondrial network 显示文摘 | Zhou L Aon MA Almas T | 2010 | PLoSComput Biol2010，6，1： | 1 |
| 9 | A Proteomics Study of Brassinosteroid Response in Arabidopsis显示文摘 | DENG Z P ZHANG X TANG W Q OSES-PRIETO J A SUZUKI N GENDORN J M CHEN H J GUAN S H CHALKLEY R J PETERMAN T K ALMA L B WANG Z Y | 2007 | Molecular & Cellular Proteomics2007，6，： | 1 |
| 10 | Burlingame analysis ofleaf proteome after UV-B irradiation in maize lines differing insensitivity显示文摘 | Casati Paula Zhang Xing L Alma | 2005 | Molecular&Cellular Proteomics2005，4，： | 1 |
| 11 | Explorations of negotiation behaviors in ten foreign cultures using a model developed in the united states 显示文摘 | Graham L John Alma T Mintu Waymond Rodgers | 1994 | Management Science1994，40，1： | 1 |
| 12 | Inactivation of Alicyclobacillusacidoterrestris vegetative cells in model system,apple,orangeand tomato juice by high hydrostaticpressure显示文摘 | Alpsh H Alma L Bozoglu F | 2003 | WorldMicrobiol&Biotech2003，19，： | 1 |
| 13 | Inactivation of Alicyclobacillus acidoterrestris vegetative cells in model system, apple, orange and tomato juices by high hydrostatic pressure显示文摘 | Alpas H Alma L Bozoglu F | 2003 | World J Microbiol Biotechnol2003，19，6： | 1 |
| 14 | Inactivation of Alicyclobacillus acidoterrestris vegetative cells in model system, apple, orange and tomato juices by high hydrostatic pressure 显示文摘 | ALPAS H ALMA L BOZOGLU F | 2003 | World Journal of Microbiology and Biotechnology2003，19，6： | 1 |
| 15 | Interaction between β-lactoglobulin and lactase and its effect on enzyme activity显示文摘 | Jimenez G J Sarabia L C Alma E | 2006 | International Dairy Journal2006，16，10： | 1 |
| 16 | Synthesis, characterization and antibacterial activity of some triasole Mannich bases carrying diphenylsulfone moieties 显示文摘 | Almajan G L Barbuceanu S F Alma jan E R | 2009 | Eur J Med Chem2009，44，7： | 1 |
| 17 | A novel Bacteroidetes symbiont is localized in Scaphoideus titanus, the insect vector of Flavescence dor6e in Vitis vinifer 显示文摘 | Marzorati M Alma A Sacchi L | 2006 | Appl Environ Microbiol2006，72，2： | 1 |
| 18 | Sand and synthetic medium filtration of advanced primary treatment effluent from Mexico city显示文摘 | Blanca J Alma C Alberto L | 2000 | Water Research2000，34，2： | 1 |
| 19 | An efficient microwave - assisted method to obtain 5 - nitrofurfural without solvents on mineral solid supports 显示文摘 | PEREZ E R MARRERO ALMA L | 1995 | Tetrahedron Lett1995，36，11： | 1 |
| 20 | A reaction-diffusion model of ROS-induced ROS release in a mitochondrial network显示文摘 | Zhou L Aon MA Almas T | | 0，，01： | 1 |
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