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| 1 | Mapping of metastasis suppressor genes for prostate cancer by microcell-mediated chromosome transfer显示文摘Aim: To identify the metastasis suppressor genes for prostate cancer. Methods: A copy of human chromosomes was introduced into the highly metastatic Dunning R-3327 rat prostate cancer cells by the use of microcell-mediated chromosome transfer. Relationships between the size of human chromosomes introduced into microcell hybrid clones and the number of lung metastases produced by the clones were analyzed to determine which part of human chromosomes contained the metastasis suppressor gene (s) for prostate cancer. To determine portions of human chromosomes introduced, G-banding chromosomal analysis, fluorescence in situ hybridization analysis, and polymerase chain reaction analysis were performed. Results: Each of microcell hybrid clones containing human chromosomes 7, 8, 10, 11, 12, or 17 showed decreased ability to metastasize to the lung without any loss of ttmaorigenicity. This demonstrates that these human chromosomes contain metastasis suppressor genes for prostate cancer. Spontaneous deletion of portions of human chromosomes was observed in the human chromosome 7, 10, 11, 12, and 17 studies. In the human chromosome 8 study, irradiated microcell-mediated chromosome transfer was performed to enrich chromosomal ann deletions of human chromosome 8. Molecular and cytogenetic analyses of microcell hybrid clones demonstrated that metastasis suppressor genes on human chromosomes were located on 7q21-22, 7q31.2-32, 8p21-12, 10q11-22, 11p13-11.2, 12p11-q13, 12q24-ter, and 17pter-q23. KAI1 and MKK4/SEKI were identified as metastasis suppressor genes from 11p11.2 and 17p12, respectively. Conclusion: This assay system is useful to identify metastasis suppressor gene (s) for prostate cancer. | Tomohiko ICHIKAWA Shigeru HOSOKI Hiroyoshi SUZUKI Koichiro AKAKURA Tatsuo IGARASHI Yuzo FURUYA Mitsuo OSHIMURA Carrie W.RINKER-SCHAEFFER Naoki NIHEI J.Carl BARRETT John T.ISAACS Haruo ITO | 2000 | Asian Journal of Andrology2000，2，3： | 2 |
| 2 | Loss of the SSeCKS/ Gravin/AKAP12 gene results in prostatic hyperplasia 显示文摘 | Akakura S Huang C Nelson PJ | 2008 | Cancer Res2008，68，13： | 1 |
| 3 | Effects of intermittent androgen suppression on the stem cell composition and the expression of the TRPM-2 (elusterin) gene in the Shionogi careinoma显示文摘 | Akakura K Bruchovsky N Rennie PS | 1996 | J Steroid Bioehem Mol Biol1996，59，56： | 1 |
| 4 | Constitutive expression of hypoxia- inducible factor- 1 alpha renders pancreatic cancer cells resistant to apoptosis induced by hypoxia and nutrient deprivation 显示文摘 | Akakura N Kobayashi M Horiuchi I | 2001 | Cancer Res2001，61，17： | 1 |
| 5 | Effects of intermittent androgen suppression on androgen-dependent tumors: apoptosis and serum prostate-specific antigen显示文摘 | Akakura K Bruchovsky N Goldenberg SL | 1993 | Cancer1993，71，9： | 1 |
| 6 | Value of f/tPSA ratio for detection and staging of PCa显示文摘 | furuya Y Akakura k lto H | 2000 | International Joumal of Clinical Oncology2000，5，1： | 1 |
| 7 | Constitutive expression of hypoxia-inducible factor 1 alpha renders pancreatic cancer cells resistant to apoptosis induced by hypoxia and nutrient deprivation显示文摘 | Akakura N Kobayashi M Horiuchi I | 2001 | Cancer Res2001，61，17： | 1 |
| 8 | Effects of temperature and inoculum on the degradability of poly(e-caprolactone)during compoating显示文摘 | Akihito Ohtaki Naoki Akakura Kiyohiko Nakasaki | 1998 | Polym Degrad Stab1998，62，1： | 1 |
| 9 | Stereose1ective ring?opening polymerization of racemic lac tide usingaluminum -achiral ligand complexes: exploration of a chain -end control mechanism显示文摘 | NOMURA N ISHII R AKAKURA M | 2002 | Journal of the American Chemical Society2002，124，： | 1 |
| 10 | Clinical usefulness of serum antip53 antibodies for prostate cancer detection: a comparative studywith prostate specific antigen parameters 显示文摘 | Suzuki H Akakura K Igarashi T | 2004 | J Urol2004，171，1： | 1 |
| 11 | The opsonin MFG-E8 is a ligand for the alphavbeta5 integrin and triggers DOCK180-dependent Racl activation for the phagocytosis of apoptotic cells 显示文摘 | Akakura S Singh S Spataro M | 2004 | Exp Cell Res2004，292，2： | 1 |
| 12 | A role for schwann cells in the neuroregenerative effects of a non-immunosuppressive FK506 derivative, JNJ460 显示文摘 | Birge RB Wadsworth S Akakura R | 2004 | Neuroscience2004，124，2： | 1 |
| 13 | Efficacy of Covered Metallic Stents in the Treatment of Unresectable Malignant Biliary Obstruction显示文摘 | Shiro Miyayama Osamu Matsui Yukari Akakura Toru Yamamoto Hiroto Nishida Kenji Yoneda Keiichi Kawai Daisyu Toya Nobuyoshi Tanaka Takeshi Mitsui Yasuyuki Asada | 2004 | CardioVascular and Interventional Radiology2004，，： | 1 |
| 14 | Outcomes of shockwave lithotripsy for upper urinary-tract stones: a large-scale study at a single institution显示文摘 | Abe T Akakura K Kawaguchi M | 2005 | J Endourol2005，19，7： | 1 |
| 15 | Outcomes of shockwavelithotripsy for upper urinary-tract stones:a large-scale study at asingle institution显示文摘 | Abe T Akakura K Kawaguchi M | 2005 | J Endourol Sep2005，19，7： | 1 |
| 16 | Pretreatment serum level ofneuron specific enolase (NSE) as a prognostic factor in metastatic prostate cancer patients treated with endocrine therapy显示文摘 | Kamiya N Akakura K Suzuki H | 2003 | European Urology2003，44，3： | 1 |
| 17 | Pro-inflammatory secretory phospholipase A2 type ⅡA binds to intepins αvβ3 and α4β1 and induces proliferation of monocytic cells in an integrin dependent manner显示文摘 | Saequsa J Akakura N Wu CY | 2008 | Biology Chemical2008，283，26： | 1 |
| 18 | Effectsof temperature and inoculum on the degradability ofpolycaprolactone during composting显示文摘 | Akihito Ohtaki Naoki Akakura Kiyohiko Nakasaki | 1998 | Polymer Degradation and Stability1998，62，： | 1 |
| 19 | Consti- tutive expression of hypoxia-inducible factor-lalpha renders pancreatic cancer cells resistant to apoptosis induced by hypoxia and nutrient deprivation显示文摘 | Akakura N Kobayashi M Horiuehi I et ol | 2001 | Cancer Res2001，61，17： | 1 |
| 20 | Constitutive ex- pression of hypoxia - inducible factor - 1 alpha renders pan- creatic cancer cells resistant to apoptosis induced by hypoxia and nutrient deprivation 显示文摘 | Akakura N Kobayashi M Horiuehi I | 2001 | Cancer Res2001，61，17： | 1 |
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