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| 1 | Design of 16S rRNA gene primers for 454 pyrosequencing of the human foregut microbiome显示文摘AIM:To design and validate broad-range 16S rRNA primers for use in high throughput sequencing to classify bacteria isolated from the human foregut microbiome.METHODS:A foregut microbiome dataset was constructed using 16S rRNA gene sequences obtained from oral,esophageal,and gastric microbiomes produced by Sanger sequencing in previous studies represented by 219 bacterial species.Candidate primers evaluated were from the European rRNA database.To assess the effect of sequence length on accuracy of classification,16S rRNA genes of various lengths were created by trimming the full length sequences.Sequences spanning various hypervariable regions were selected to simulate the amplicons that would be obtained using possible primer pairs.The sequences were compared with full length 16S rRNA genes for accuracy in taxonomic classification using online software at the Ribosomal Database Project (RDP).The universality of the primer set was evaluated using the RDP 16S rRNA database which is comprised of 433 306 16S rRNA genes,represented by 36 phyla.RESULTS:Truncation to 100 nucleotides(nt)downstream from the position corresponding to base 28 in the Escherichia coli 16S rRNA gene caused misclassification of 87(39.7%)of the 219 sequences,compared with misclassification of only 29(13.2%)sequences with truncation to 350 nt.Among 350-nt sequence reads within various regions of the 16S rRNA gene,the reverse read of an amplicon generated using the 343F/798R primers had the least(8.2%)effect on classification.In comparison,truncation to 900 nt mimicking single pass Sanger reads misclassified 5.0%of the 219 sequences.The 343F/798R amplicon accurately assigned 91.8%of the 219 sequences at the species level.Weighted by abundance of the species in the esophageal dataset,the 343F/798R amplicon yielded similar classification accuracy without a significant loss in species coverage(92%).Modification of the 343F/798R primers to 347F/803R increased their universality among foregut species.Assuming that a typicalpolymerase chain reaction can tolerate 2 mismatches between a primer and a template,the modified 347F and 803R primers should be able to anneal 98%and 99.6%of all 16S rRNA genes in the RDP database.CONCLUSION:347F/803R is the most suitable pair of primers for classification of foregut 16S rRNA genes but also possess universality suitable for analyses of other complex microbiomes. | Carlos W Nossa William E Oberdorf Jφrn A Aas Bruce J Paster Todd Z DeSantis Eoin L Brodie Daniel Malamud Michael A Poles Zhiheng Pei | 2010 | World Journal of Gastroenterology2010，16，33： | 16 |
| 2 | Risk factors for post-ERCP pancreatitis: A prospective, multicenter study显示文摘 | Martin L. Freeman James A. DiSario Douglas B. Nelson M.Brian Fennerty John G. Lee David J. Bjorkman Carol S. Overby Johannes Aas Michael E. Ryan Gary S. Bochna Michael J. Shaw Harry W. Snady Robert V. Erickson Joseph P. Moore Joseph P. Roel | 2001 | Gastrointestinal Endoscopy2001，，4： | 5 |
| 3 | Pair-copula constructions of multiple dependence显示文摘 | Kjersti Aas Claudia Czado Arnoldo Frigessi Henrik Bakken | 2007 | Insurance Mathematics and Economics2007，，2： | 4 |
| 4 | Long-term prognosis in patients with severe late radiation enteropathy:A prospective cohort study显示文摘AIM: To assess persistent symptoms and mortality in a cohort of patients with severe (grade 3-4) radiation enteropathy,59 patients were followed up after 15-18 years. METHODS: Fifty-nine patients were prospectively enrolled by twelve surgical departments. Primary malignant disease,radiation therapy and surgical management were recorded at inclusion. The cause of death or persistence of symptoms was examined in public death records or by interview of survivors. RESULTS: Thirty-nine patients had received radiation therapy for gynaecological cancers,twelve for urological cancers,four for gastrointestinal cancers and four for other malignancies. Forty-five patients (76%) required surgical intervention. Complications occurred in 11 (25%) operated patients. Forty-seven patients had died at the time of follow-up,seven (12%) died as a direct result of radiation enteropathy,while radiation enteropathy contributed to death in an additional seven patients. Four of the twelve surviving patients suffered from chronic debilitating symptoms of radiation enteropathy,while three had moderate symptoms. CONCLUSION: Patients with severe delayed radiation enteropathy have a high risk of persistence of symptoms after surgery. At least one in ten patients dies from radiation-induced bowel injury. | Annette Larsen Jon B Reitan Steinar T Aase Martin Hauer-Jensen | 2007 | World Journal of Gastroenterology2007，13，26： | 4 |
| 5 | Wheat response and residual soil properties following subsoiling of a sandy loa in eastern Montana 显示文摘 | Pikol J L Aase J K | 1999 | Soil & Tillage Research1999，51，1： | 2 |
| 6 | Wheat response and residual soil properties following subsoiling of a sandy loam in eastern Montana显示文摘 | J.L Pikul J.K Aase | 1999 | Soil & Tillage Research1999，，1： | 2 |
| 7 | Risk factors for post-ERCP pancreatitis: A prospective, multicenter study显示文摘 | Martin L. Freeman James A. DiSario Douglas B. Nelson M.Brian Fennerty John G. Lee David J. Bjorkman Carol S. Overby Johannes Aas Michael E. Ryan Gary S. Bochna Michael J. Shaw Harry W. Snady Robert V. Erickson Joseph P. Moore Joseph P. Roel | 2001 | Gastrointestinal Endoscopy2001，，4： | 2 |
| 8 | Laboratory determination of strength properties of frozen salt marine clay显示文摘 | AAS G | 1981 | Engineering Geology1981，18，： | 1 |
| 9 | Vascular endothelial growth factor B (VEGF-B) binds to VEGF receptor-1 and regulates plasminogen activator activity in endothelial cells显示文摘 | Olofsson B Korpelainen E Pepper MS Mandriota SJ Aase K Kumar V | 1998 | Proc Natl Acad Sci USA1998，95，11： | 1 |
| 10 | Biochemical analysis ofblood-reference values for sows 显示文摘 | Framstad T Morberg H Aas R A | 1991 | Nor Vet Tidsskr1991，103，： | 1 |
| 11 | Tissue-engineered conduit using urine-derived stem cells seeded bacterial cellulose polymer in urinary reconstruction and diversion显示文摘 | Aase Bodin Shantaram Bharadwaj Shaofeng Wu Paul Gatenholm Anthony Atala Yuanyuan Zhang | 2010 | Biomaterials2010，，： | 1 |
| 12 | Multisite analyses of spectral- biophysical data for wheat 显示文摘 | Wiegand C L Maas S J Aase J K | 1992 | Remote Sensing of Environment1992，42，： | 1 |
| 13 | PDGF-C is a new protease-activated ligand for the PDGF alpha-receptor 显示文摘 | Li X Ponten A Aase K | 2000 | Nat Cell Biol2000，2，5： | 1 |
| 14 | PDGF-C is a new protease-activated ligand for the PDGF α-receptor显示文摘 | Li X Ponten A Aase K | 2003 | Nat Cell Biol2003，2，5： | 1 |
| 15 | PDGF-C is a new protease-activated ligand for the PDGF a-receptor显示文摘 | Li X Ponten A Aase K | 2000 | Nat Cell Biol2000，2，5： | 1 |
| 16 | The effect of grain- coating microquartz on preservation of reservoir porosity显示文摘 | Aase N E Bjorkum A Nadeau P H | 1996 | AAPG Bulletin1996，80，： | 1 |
| 17 | Water infiltration and storage affected by subsoiling and subseauent tillage 显示文摘 | Pikul Jr J L Aase J K | 2003 | Soil Science Society of American Joranal2003，67，3： | 1 |
| 18 | Proton magnetic resonce spectroscopy of brain biopsies from patients with intractable epilepsy显示文摘 | Aasly J Silfzenius H Aas TC | 1999 | Epilepsy Res1999，35，： | 1 |
| 19 | PAH metabolites in bile, cytochrome P4501A and DNA addncts as environmental risk parameters for chronic oil exposure: a laboratory experiment with Atlantic cod显示文摘 | AAS E BAUSSANT T BALK L | 2000 | Aquat Toxicol2000，51，2： | 1 |
| 20 | Skeletal muscle lipid accumulation in type 2 diabetes may involve the liver X receptor pathway显示文摘 | Kase ET Wensaas A J Aas V | | 0，，4： | 1 |
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