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| 1 | Pancreatic regenerating protein (regⅠ) and regⅠreceptor mRNA are upregulated in rat pancreas after induction of acute pancreatitis显示文摘AIM: Pancreatic regenerating protein (regⅠ) stimulates pancreatic regeneration after pancreatectomy and is mitogenic to ductal andβ-cells. This suggests that regⅠand its receptor may play a role in recovery after pancreatic injury. We hypothesized that regⅠand its receptor are induced in acute pancreatitis. METHODS: Acute pancreatitis was induced in male Wistar rats by retrograde injection of 3% sodium taurocholate into the pancreatic duct. Pancreata and serum were collected 12, 24, and 36 hours after injection and from normal controls (4 rats/group). RegⅠreceptor mRNA, serum regⅠprotein, and tissue regⅠprotein levels were determined by Northern analysis, enzyme-linked immunosorbent assay (ELISA), and Western analysis, respectively. Immunohistochemistry was used to localize changes in regⅠand its receptor. RESULTS: Serum amylase levels and histology confirmed necrotizing pancreatitis in taurocholate treated rats. There was no statistically significant change in serum regⅠconcentrations from controls. However, Western blot demonstrated increased tissue levels of regⅠat 24 and 36 h. This increase was localized primarily to the acinar cells and the ductal cells by immunohistochemistry. Northern blot demonstrated a significant increase in regⅠreceptor mRNA expression with pancreatitis. Immunohistochemistry localized this increase to the ductal cells, islets, and acinar cells. CONCLUSION: Acute pancreatitis results in increased tissue regⅠprotein levels localized to the acinar and ductal cells, and a parallel threefold induction of regⅠreceptor in the ductal cells, islets, and acinar cells. These changes suggest that induction of reg I and its receptor may be important for recovery from acute pancreatitis. | Martin H Bluth Sameer A Patel Brian K Dieckgraefe Hiroshi Okamoto Michael E Zenilman | 2006 | World Journal of Gastroenterology2006，12，28： | 19 |
| 2 | Acute pancreatitis in aging animals:Loss of pancreatitis-associated protein protection?显示文摘AIM:To investigate the effect of age on severity of acute pancreatitis(AP) using biochemical markers,histology and expression of the protective pancreatitisassociated proteins(PAPs).METHODS:AP was induced via intraductal injection of 4% sodium taurocholate in young and old rats.Sera and pancreata were assayed at 24 h for the parameters listed above;we also employed a novel molecular technique to assess bacterial infiltration using polymerase chain reaction to measure bacterial genomic ribosomal RNA.RESULTS:At 24 h after induction of AP,the pancreata of older animals had less edema(mean ± SE histologic score of young vs old:3.11 ± 0.16 vs 2.50 ±-0.11,P < 0.05),decreased local inflammatory response(histologic score of stromal infiltrate:3.11 ± 0.27 vs 2.00 ± 0.17,P < 0.05) and increased bacterial infiltration(174% ± 52% increase from sham vs 377% ± 4%,P < 0.05).A decreased expression of PAP1 and PAP2 was demonstrated by Western blotting analysis and immunohistochemical staining.There were no differences in serum amylase and lipase activity,or tissue myeloperoxidase or monocyte chemotactic protein-1 levels.However,in the most-aged group,serum C-reactive protein levels were higher(young vs old:0.249 ± 0.04 mg/dL vs 2.45 ± 0.68 mg/dL,P < 0.05).CONCLUSION:In older animals,there is depressed PAP expression related to a blunted inflammatory response in AP which is associated with worsened bacterial infiltration and higher C-reactive protein level;this may explain the more aggressive clinical course. | Sophia Fu Albert Stanek Cathy M Mueller Nefertti A Brown Chongmin Huan Martin H Bluth Michael E Zenilman | 2012 | World Journal of Gastroenterology2012，18，26： | 5 |
| 3 | Quantitative analysis ofgenetically modified organisms (GMO) in processed food by PCR- based methods显示文摘 | Wurz A Bluth A Zeltz P etal | 1999 | Food Control1999，10，： | 1 |
| 4 | Quantitative Analysis of Genetically Modified Organisms(GMO) in Processed Food by PCR-Based Methods显示文摘 | A Bluth | 1999 | Food Control1999，10，： | 1 |
| 5 | Plasmacytoma of the testis显示文摘 | Walker FB Bluth EI Kenney A | 2005 | J Ultrasound Med2005，24，12： | 1 |
| 6 | Validation of a method based on polymerase chain reaction for the detection of genetically modified organisms in various processed foodstuffs显示文摘 | Lipp M Bluth A Eyquem F | 2001 | Eur Food Res Technol2001，212，： | 1 |
| 7 | 2012 key data elements and definitions for peripheral atherosclcrotic vascular disease: a report of the American College of Cardiology Foundafion/Amedcan Heart Association Task Force on Clinical Data Standards (Writing Committee to Develop Clinical Data Standards for Peripheral Atherosclerotic Vascular Disease) 显示文摘 | Creagcr MA Belkin M Bluth EI ACCF/AHA/A | 2012 | Circulation2012，125，2： | 1 |
| 8 | Transport of brain-derived neurotrophic factor across the blood–brain barrier显示文摘 | Weihong Pan William A Banks Melita B Fasold Jonathan Bluth Abba J Kastin | 1998 | Neuropharmacology1998，，12： | 1 |
| 9 | Equation of State Calculations by Fast Computing Machines显示文摘 | METROPOLIS N ROSENBLUTH A W ROSEN- BLUTH M N | 1953 | Journal of Chemi- cal Physics1953，21，6： | 1 |
| 10 | Mechanisms of the behavioural effects of cytokines显示文摘 | Dantzer R Aubert A Bluthe RM | 1999 | Adv Exp Med Biol1999，461，： | 1 |
| 11 | Equation of state calculations by fast computing machines 显示文摘 | METROPOLIS N ROSENBLUTH A ROSEN- BLUTH M | 1953 | Journal of Chemical Physics1953，2，： | 1 |
| 12 | Anesthesia in endourological and robot-assisted interventions显示文摘 | Kiss T Bluth T Heller A | 2012 | Anaesthesist2012，61，8： | 1 |
| 13 | Central injection of interleukin-13 potentiates LPS induced sickness behavior in rats显示文摘 | Bluthe R M Bristow A Lestage J | 2001 | Neuroreport2001，12，： | 1 |
| 14 | Cancer/testis antigens,game to genesis and cancer显示文摘 | Simpson AJ Caballero OL Jung bluth A | | 0，，： | 1 |
| 15 | Quantitative analysis of genetically modified organisms (GMO) in processed food by PCR-based methods 显示文摘 | Wurz A Bluth A Zeltz P | 1999 | Food Contr1999，10，： | 1 |
| 16 | Experimental invesigations of steady flow in rigid models of abdominal aortic aneurysms显示文摘 | Asbury C L Ruberti J W Bluth E I Peattie R A | 1995 | Journal of Biomedical Engineering1995，23，： | 1 |
| 17 | Quantitative analysis of genetically modified organisms (GMO) in processed food by PCR-based methods显示文摘 | Wurz A Bluth P Zeltz C | 1999 | Food Control1999，10，： | 1 |
| 18 | Validation of a method based on polymerase chain reaction for the detection of genetically modified organisms in various processed foodstuffs 显示文摘 | Lipp M Bluth A Eyquem F | 2006 | Eur Food Res Technol2006，212，4： | 1 |
| 19 | Plasmacyoma of the testis显示文摘 | Walker F B Bluth E L Kenney A | 2005 | J Ultrasound Med2005，24，12： | 1 |
| 20 | Effects of ultraprotective ventilation, extracorporeal Carbon dioxide removal, and spontaneous breathing on lung morphofunction and inflammation in experimental severe acute respiratory distress syndrome显示文摘 | Güldner A Kiss T Bluth T | 2015 | Anesthesiology2015，122，3： | 1 |
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