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| 1 | Pancreatic regenerating protein (regⅠ) and regⅠreceptor mRNA are upregulated in rat pancreas after induction of acute pancreatitis显示文摘AIM: Pancreatic regenerating protein (regⅠ) stimulates pancreatic regeneration after pancreatectomy and is mitogenic to ductal andβ-cells. This suggests that regⅠand its receptor may play a role in recovery after pancreatic injury. We hypothesized that regⅠand its receptor are induced in acute pancreatitis. METHODS: Acute pancreatitis was induced in male Wistar rats by retrograde injection of 3% sodium taurocholate into the pancreatic duct. Pancreata and serum were collected 12, 24, and 36 hours after injection and from normal controls (4 rats/group). RegⅠreceptor mRNA, serum regⅠprotein, and tissue regⅠprotein levels were determined by Northern analysis, enzyme-linked immunosorbent assay (ELISA), and Western analysis, respectively. Immunohistochemistry was used to localize changes in regⅠand its receptor. RESULTS: Serum amylase levels and histology confirmed necrotizing pancreatitis in taurocholate treated rats. There was no statistically significant change in serum regⅠconcentrations from controls. However, Western blot demonstrated increased tissue levels of regⅠat 24 and 36 h. This increase was localized primarily to the acinar cells and the ductal cells by immunohistochemistry. Northern blot demonstrated a significant increase in regⅠreceptor mRNA expression with pancreatitis. Immunohistochemistry localized this increase to the ductal cells, islets, and acinar cells. CONCLUSION: Acute pancreatitis results in increased tissue regⅠprotein levels localized to the acinar and ductal cells, and a parallel threefold induction of regⅠreceptor in the ductal cells, islets, and acinar cells. These changes suggest that induction of reg I and its receptor may be important for recovery from acute pancreatitis. | Martin H Bluth Sameer A Patel Brian K Dieckgraefe Hiroshi Okamoto Michael E Zenilman | 2006 | World Journal of Gastroenterology2006，12，28： | 19 |
| 2 | Acute pancreatitis in aging animals:Loss of pancreatitis-associated protein protection?显示文摘AIM:To investigate the effect of age on severity of acute pancreatitis(AP) using biochemical markers,histology and expression of the protective pancreatitisassociated proteins(PAPs).METHODS:AP was induced via intraductal injection of 4% sodium taurocholate in young and old rats.Sera and pancreata were assayed at 24 h for the parameters listed above;we also employed a novel molecular technique to assess bacterial infiltration using polymerase chain reaction to measure bacterial genomic ribosomal RNA.RESULTS:At 24 h after induction of AP,the pancreata of older animals had less edema(mean ± SE histologic score of young vs old:3.11 ± 0.16 vs 2.50 ±-0.11,P < 0.05),decreased local inflammatory response(histologic score of stromal infiltrate:3.11 ± 0.27 vs 2.00 ± 0.17,P < 0.05) and increased bacterial infiltration(174% ± 52% increase from sham vs 377% ± 4%,P < 0.05).A decreased expression of PAP1 and PAP2 was demonstrated by Western blotting analysis and immunohistochemical staining.There were no differences in serum amylase and lipase activity,or tissue myeloperoxidase or monocyte chemotactic protein-1 levels.However,in the most-aged group,serum C-reactive protein levels were higher(young vs old:0.249 ± 0.04 mg/dL vs 2.45 ± 0.68 mg/dL,P < 0.05).CONCLUSION:In older animals,there is depressed PAP expression related to a blunted inflammatory response in AP which is associated with worsened bacterial infiltration and higher C-reactive protein level;this may explain the more aggressive clinical course. | Sophia Fu Albert Stanek Cathy M Mueller Nefertti A Brown Chongmin Huan Martin H Bluth Michael E Zenilman | 2012 | World Journal of Gastroenterology2012，18，26： | 5 |
| 3 | Dexamethasone mediates protection against acute pancreatitis via upregulation of pancreatitis-associated proteins显示文摘AIM: To examine the influence of dexamethasone on pancreatitis-associated protein (PAP) gene expression using both in vitro and in vivo models of acute pancreati- tis and to study how PAP gene expression correlates with severity of pancreatitis. METHODS: In vitro, IL-6 stimulated pancreas acinar AR42J cells were cultured with increasing concentrations of dexamethasone and assayed for PAP expression (RT-PCR). In vivo , pancreatitis was induced in rats by retrograde injection of 40 g/L taurocholate into the pancreatic duct. Animals were pretreated with dexamethasone (2 mg/kg) daily or saline for 4 d. Pancreata and serum were harvested after 24 h and gene expression levels of PAPⅠ, Ⅱ and Ⅲ were measured by RT-PCR. Severity of pancreatitis was based on serum amylase, pancreatic wet weight, and histopathological score. RESULTS: In vitro, dexamethasone and IL-6 induced a marked transcription of PAPⅠ, Ⅱ and Ⅲ genes in AR42J cells at 24 h (P < 0.05 for all comparisons). In vivo, pancreas mRNA levels of PAPⅠ, Ⅱ or Ⅲ increased by 2.6-fold, 1.9-fold, and 1.3-fold respectively after dexa- methasone treatment, compared with saline treated ani- mals. Serum amylase levels and edema were significantly lower in the dexamethasone group compared with the saline group. Histopathologic evaluation revealed less inflammation and necrosis in pancreata obtained from dexamethasone treated animals (P < 0.05). CONCLUSION: Dexamethasone significantly decreases the severity of pancreatitis. The protective mechanism ofdexamethasone may be via upregulating PAP gene ex- pression during injury. | Emad Kandil Yin-Yao Lin Martin H Bluth Hong Zhang Gabriel Levi Michael E Zenilman | 2006 | World Journal of Gastroenterology2006，12，42： | 3 |
| 4 | 冷能利用片冰机海水淡化动态仿真显示文摘介绍了一种新型的冷冻海水淡化技术。到2019年末,液化天然气(LNG)生产能力已达4.3亿吨/年。LNG从再气化过程中释放出的巨大冷能量可用于冷冻脱盐过程,以最大限度地降低总能耗。本文采用HYSYS软件设计并仿真了利用LNG冷能的片冰机冷冻海水淡化(FD)过程。采用片冰机上的制冰桶作为海水结晶器,主要是由于其连续制冰和除冰无热源。利用gPROMS软件建立了冻结段的动态模型并进行了仿真。结果表明,用1kg当量液化天然气冷能可获得1.9~2.1kg的冰融水,该混合工艺的制冷剂泵功率能耗为3.725Wh/100kg,可忽略不计。 | 曹文胜 徐建壮 郭兆春 林文胜 BLUTH Christoph | 2021 | 化工进展2021，40，S01： | 2 |
| 5 | Evaluation and characterization of carotid plaque显示文摘 | Bluth E | 1977 | Seminars in Ultrasoud CT MRl1977，18，： | 1 |
| 6 | Carotid duplex sonography: a multicenter recommendation for standardized imaging and Doppler criteria显示文摘 | Bluth EI Stavros AT Marich KW | 1988 | Radiographics1988，8，3： | 1 |
| 7 | Use of gene expression profiles in cells of peripheral blood to identify new molecular markers of acute pancreatitis显示文摘 | Bluth M Lin YY Zhang H | 2008 | Arch Surg2008，143，3： | 1 |
| 8 | 2012 ACCF/AHA/ ACR/SCAI/SIR/STS/SVM/SVN/SVS Key data elements and definitions for peripheral atherosclerotic vascular disease : a report of the American College of Cardiology Foundation/American Heart Association Task Force on Clinical Data Standards (Writing Committee to develop Clinical Data Standards for peripheral atherosclerotic vascular disease) 显示文摘 | Creager MA Belkin M Bluth EI | 2012 | J Am Coil Cardiol2012，59，3： | 1 |
| 9 | Evaluation and characterization of carotid plaque显示文摘 | Bluth EI | 1997 | Semin Ultrasound CT MRI1997，18，： | 1 |
| 10 | Chronic treatment with the atypical antidepressant tianeptine attenuates sickness behavior induced by peripheral but not central lipopolysaccharide and interleukin-1beta in the rat显示文摘 | Castanon N Bluthe RM Dantzer R | 2001 | Psychopharmacology (Berl)2001，154，1： | 1 |
| 11 | Delayed posterior dislocation of silicone plate-haptic lenses after neodymium:yag capsulotomy显示文摘 | Petersen AM Bluth LL Campion M | 2000 | Journal of cataract and refractive surgery2000，26，12： | 1 |
| 12 | Mindfulness and self-compassion:Exploring pathways to adolescent emotional well-being显示文摘 | Bluth K Blanton P W | 2014 | Journal of Child and Family Studies2014，23，7： | 1 |
| 13 | Early postoperative hepatic sonography as a predictor of vascular and biliary complications in adult orthotopic liver transplant patients显示文摘 | Uzochukwu LN Bluth EI Smetherman DH | 2005 | AJR2005，185，5： | 1 |
| 14 | Use of top-level structure in text: key for reading comprehension of ninth-grade显示文摘 | MEYER B BRANDT D BLUTH G | 1980 | Reading Research Quarterly1980，15，1： | 1 |
| 15 | The influence of self-compassion on emotional well-being among early and older adolescent males and females显示文摘 | Bluth K Blanton P W | 2015 | The Journal of Positive Psychology2015，10，3： | 1 |
| 16 | Quantitative analysis ofgenetically modified organisms (GMO) in processed food by PCR- based methods显示文摘 | Wurz A Bluth A Zeltz P etal | 1999 | Food Control1999，10，： | 1 |
| 17 | Cytokine effects on behavior显示文摘 | Dantzer R Bluthe RM Castanon N | 2001 | Psychoneuroimmunology2001，3，： | 1 |
| 18 | Lithological and climatological controls of river chemistry显示文摘 | Bluth G J S Kump L R | 1994 | Geochimica et Cosmochimica Acta1994，58，： | 1 |
| 19 | R?ntgenographic measu- rements of expansion of crystalline masses 显示文摘 | BüSSEM W BLUTH M GROCHMANN G | 1935 | Ber Deut Keram Ges1935，16，： | 1 |
| 20 | Sonographic characterization of carotid plaque 显示文摘 | Bluth EL | 1986 | AJNR1986，7，2： | 1 |
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