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| 1 | Targeted Mutagenesis in Zea mays Using TALENs and the CRISPR/Cas System显示文摘Transcription activator-like effector nucleases(TALENs) and clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated(Cas) systems have emerged as powerful tools for genome editing in a variety of species. Here, we report, for the first time, targeted mutagenesis in Zea mays using TALENs and the CRISPR/Cas system. We designed five TALENs targeting 4 genes, namely ZmPDS, ZmIPK1A, ZmIPK, ZmMRP4, and obtained targeting efficiencies of up to 23.1% in protoplasts, and about 13.3% to 39.1% of the transgenic plants were somatic mutations. Also, we constructed two gRNAs targeting the ZmIPK gene in maize protoplasts, at frequencies of 16.4% and 19.1%, respectively. In addition, the CRISPR/Cas system induced targeted mutations in Z. mays protoplasts with efficiencies(13.1%) similar to those obtained with TALENs(9.1%). Our results show that both TALENs and the CRISPR/Cas system can be used for genome modification in maize. | Zhen Liang Kang Zhang Kunling Chen Caixia Gao | 2014 | Journal of Genetics and Genomics2014，41，2： | 136 |
| 2 | Genome-wide analysis of heat shock transcription factor families in rice and Arabidopsis显示文摘The heat shock transcription factors (HSFs) are the major heat shock factors regulating the heat stress response. They participate in regulating the expression of heat shock proteins (HSPs), which are critical in the protection against stress damage and many other important biological processes. Study of the HSF gene family is important for understanding the mechanism by which plants respond to stress. The completed genome sequences of rice (Oryza sativa) and Arabidopsis (Arabidopsis thaliana) constitute a valuable resource for comparative genomic analysis, as they are representatives of the two major evolutionary lineages within the angiosperms: the monocotyledons and the dicotyledons. The identification of phylogenetic relationships among HSF proteins in these species is a fundamental step to unravel the functionality of new and yet uncharacterized genes belonging to this family.In this study, the full complement of HSF genes in rice and Arabidopsis has probably been identified through the genome-wide scan. Phylogenetic analyses resulted in the identification of three major clusters of orthologous genes that contain members belonging to both species, which must have been represented in their common ancestor before the taxonomic splitting of the angiosperms. Further analysis of the phylogenetic tree reveals a possible dicot specific gene group. We also identified nine pairs of paralogs, as evidence for studies on the evolution history of rice HSF family and rice genome evolution. Expression data analysis indicates that HSF proteins are widely expressed in plants. These results provide a solid base for future functional genomic studies of the HSF gene family in rice and Arabidopsis. | Jingkang Guo Jian Wu Qian Ji Chao Wang Lei Luo Yi Yuan Yonghua Wang Jian Wang | 2008 | Journal of Genetics and Genomics2008，35，2： | 52 |
| 3 | Cytological analysis and genetic control of rice anther development显示文摘Microsporogenesis and male gametogenesis are essential for the alternating life cycle of flowering plants between diploid sporophyte and haploid gametophyte generations.Rice(Oryza saliva) is the world's major staple food,and manipulation of pollen fertility is particularly important for the demands to increase rice grain yield.Towards a better understanding of the mechanisms controlling rice male reproductive development,we describe here the cytological changes of anther development through 14 stages,including cell division,differentiation and degeneration of somatic tissues consisting of four concentric cell layers surrounding and supporting reproductive cells as they form mature pollen grains through meiosis and mitosis.Furthermore,we compare the morphological difference of anthers and pollen grains in both monocot rice and eudicot Arabidopsis thaliana.Additionally,we describe the key genes identified to date critical for rice anther development and pollen formation. | Dabing Zhang Xue Luo Lu Zhu | 2011 | Journal of Genetics and Genomics2011，38，9： | 56 |
| 4 | TALEN or Cas9-Rapid,Efficient and Specific Choices for Genome Modifications显示文摘Precise modifications of complex genomes at the single nucleotide level have been one of the big goals for scientists working in basic and applied genetics,including biotechnology,drug development,gene therapy and synthetic biology.However,the relevant techniques for making these manipulations in model organisms and human cells have been lagging behind the rapid high throughput studies in the post-genomic era with a bottleneck of low efficiency,time consuming and laborious manipulation,and off-targeting problems.Recent discoveries of TALEs(transcription activator-like effectors) coding system and CRISPR(clusters of regularly interspaced short palindromic repeats) immune system in bacteria have enabled the development of customized TALENs(transcription activator-like effector nucleases) and CRISPR/Cas9 to rapidly edit genomic DNA in a variety of cell types,including human cells,and different model organisms at a very high efficiency and specificity.In this review,we first briefly summarize the development and applications of TALENs and CRISPR/Cas9-mediated genome editing technologies;compare the advantages and constraints of each method;particularly,discuss the expected applications of both techniques in the field of site-specific genome modification and stem cell based gene therapy;finally, propose the future directions and perspectives for readers to make the choices. | Chuanxian Wei Jiyong Liu Zhongsheng Yu Bo Zhang Guanjun Gao Renjie Jiao | 2013 | Journal of Genetics and Genomics2013，40，6： | 52 |
| 5 | Transgenic expression of MYB15 confers enhanced sensitivity to abscisic acid and improved drought tolerance in Arabidopsis thaliana显示文摘Abiotic stresses cause serious crop losses. Knowledge on genes functioning in plant responses to adverse growth conditions is essential for developing stress tolerant crops. Here we report that transgenic expression of MYB15, encoding a R2R3 MYB transcription factor in Arabidopsis thaliana, conferred hypersensitivity to exogenous abscisic acid (ABA) and improved tolerance to drought and salt stresses. The promoter of MYB15 was active in not only vegetative and reproductive organs but also the guard cells of stomata. Its transcript level was substantially upregulated by ABA, drought or salt treatments. Compared with wild type (WT) control, MYB15 overexpression lines were hypersensitive to ABA in germination assays, more susceptible to ABA-elicited inhibition of root elongation, and more sensitive to ABA-induced stomatal closure. In line with the above findings, the transcript levels of ABA biosynthesis (ABA1, ABA2), signaling (ABI3), and responsive genes (AtADH1, RD22, RD29B, AtEM6) were generally higher in MYB15 overexpression seedlings than in WT controls after treatment with ABA. MYB15 overexpression lines displayed improved survival and reduced water loss rates than WT control under water deficiency conditions. These overexpression lines also displayed higher tolerance to NaCl stress. Collectively, our data suggest that overexpression of MYB15 improves drought and salt tolerance in Arabidopsis possibly by enhancing the expression levels of the genes involved in ABA biosynthesis and signaling, and those encoding the stress-protective proteins. | Zhenhua Ding Shiming Li Xueli An Xin Liu Huanju Qin Daowen Wang | 2009 | Journal of Genetics and Genomics2009，36，1： | 42 |
| 6 | Assessment of genetic diversity in broomcorn millet (Panicum miliaceum L.) using SSR markers显示文摘The genetic diversity of 118 accessions of broomcorn millet(Panicum miliaceum L.),collected from various ecological areas,was analyzed.Using 46 SSR(Simple Sequence Repeat) polymorphic markers from rice,wheat,oat and barley,a total of 226 alleles were found,which exhibited moderate level of diversity.The number of alleles per primer ranged from two to nine,with an average of 4.91.The range of polymorphism information content(PIC) was 0.284-0.980(average,0.793).The expected heterozygosity(He) varied from 0.346 to 0.989,with an average of 0.834.The average coefficient of the genetic similarity of SSR markers among the 118 accessions was 0.609,and it ranged from 0.461 to 0.851.The UPGMA(Unweight Pair Group Method with Arithmetic Mean) clustering analysis at the genetic similarity value of 0.609 grouped the 118 accessions into five groups.Mantel test meant that geographical origin and genetic distance presented positive correlation.The clustering results were consistent with known information on ecological growing areas.The genetic similarity coefficient of the accessions in the Loess Plateau ecotype was significantly lower than those in the other ecotypes.It indicates that the highest level of genetic diversity occurred in the Loess Plateau,which is probably the original site of Panicum miliaceum. | Xingyu Hu Jianfei Wang Ping Lu Hongsheng Zhang | 2009 | Journal of Genetics and Genomics2009，36，8： | 44 |
| 7 | A Pid3 allele from rice cultivar Gumei2 confers resistance to Magnaporthe oryzae显示文摘瑞斯强风,由 Magnaporthe oryzae 引起了,是大多数破坏疾病之一。用基于地图的策略并且在 silico 途径,我们孤立新米饭(Oryza sativa L.) Pid3 的强风抵抗等位基因,指明的 Pi25,从稳定的强风抵抗栽培变种 Gumei2。在表示上分析和互补测试证明 Pi25 授与强风抵抗到 M。oryzae 孤立 js001-20。顺序分析证明 Pi25 是有与在核苷酸位置的 Pid3 相比的单个核苷酸替换的 2772 核苷酸的 intronless 基因 459 并且述词地编码了典型卷的 coilnucleotide 绑定 siteleucine 有到 Pid3 的 100% 身份的 924 氨基酸剩余的富有的重复(CCNBSLRR ) 蛋白质通常认为的蛋白质。在 Nipponbare 的易受影响的等位基因 pi25 在核苷酸位置包含了一个胡说八道变化 2209 与 736 氨基酸剩余导致截断的蛋白质。另外,导致 10 氨基酸替换的 14 核苷酸替换在 Pi25 和在上游的 pi25 之间被识别在易受影响的等位基因的早熟的站 codon。尽管 Pi25/Pid3-mediated 抵抗的机制需要进一步被调查,等位基因的隔离将在经由转基因的途径引起节目的米饭强风抵抗便于 Pi25/Pid3 的利用,标记帮助选择。 | Jie Chen Yongfeng Shi Wenzheng Liu Rongyao Chai Yaping Fu Jieyun Zhuang Jianli Wu | 2011 | Journal of Genetics and Genomics2011，38，5： | 45 |
| 8 | Efficient and Specific Modifications of the Drosophila Genome by Means of an Easy TALEN Strategy显示文摘技术开发总是是在生物医学的研究驾驶突破的力量之一。自从托马斯·摩根的时间起,一步一步地, Drosophilists 为操作并且机能上地把开发了强大的基因工具为改进这些技术并且开发新技术的果蝇染色体,而是房间仍然大,今天特别作为生物学家开始系统地学习不同模型有机体的功能的 genomics ,包括人,以一种高产量的方式。这里,我们报导,第一次在果蝇,为借助于改进抄写在很高的效率修改果蝇染色体的一个快速、容易、高度特定的方法像使活跃之物的受动器核酸酶(TALEN ) 策略。我们利用了最近发达的单位集会策略装配设计修改黄基因的二特定的 TALEN (在性染色体上) 并且新奇正染色体的基因。TALEN 的 mRNAs 随后被注入果蝇胚胎。从 31.2% 注射 F0 肥沃的苍蝇,我们检测了包含黄基因的可继承的修正。从到可继承的修正的察觉的特定的 TALEN 的建设的全部过程能在一个月以内被完成。在果蝇的这个调停 TALEN 的染色体修正方法的潜在的应用程序被讨论。 | Jiyong Liu Changqing Li Zhongsheng Yu Peng Huang Honggang Wu Chuanxian Wei Nannan Zhu Yan Shen Yixu Chen Bo Zhang Wu-Min Deng Renjie Jiao | 2012 | Journal of Genetics and Genomics2012，39，5： | 43 |
| 9 | Rapid improvement of grain weight via highly efficient CRISPR/Cas9-mediated multiplex genome editing in rice显示文摘Most of the important agronomic traits in crop plants,such as yield,quality and stress response,are quantitative and jointly controlled by many genomic loci or major genes.Improving these complex traits depends on the combination of beneficial alleles at the quantitative trait loci(QTLs).However,the conventional cross breeding method is extremely time-consuming and laborious for pyramiding multiple QTLs.In certain cases,this approach | Rongfang Xu Yachun Yang Ruiying Qin Hao Li Chunhong Qiu Li Li Pengcheng Wei Jianbo Yang | 2016 | Journal of Genetics and Genomics2016，43，8： | 47 |
| 10 | Mapping QTLs with epistatic effects and QTL×environment interactions for plant height using a doubled haploid population in cultivated wheat显示文摘Quantitative trait loci (QTLs) for plant height in wheat (Triticum aestivum L.) were studied using a set of 168 doubled haploid (DH) lines, which were derived from the cross Huapei 3/Yumai 57. A genetic linkage map was constructed using 283 SSR and 22 EST-SSR markers. The DH population and the parents were evaluated for wheat plant height in 2005 and 2006 in Tai’an and 2006 in Suzhou. QTL analyses were performed using the software of QTLNetwork version 2.0 based on the mixed linear model. Four additive QTLs and five pairs of epistatic effects were detected, which were distributed on chromosomes 3A, 4B, 4D, 5A, 6A, 7B, and 7D. Among them, three additive QTLs and three pairs of epistatic QTLs showed QTL×environment interactions (QEs). Two major QTLs, Qph4B and Qph4D, which accounted for 14.51% and 20.22% of the phenotypic variation, were located similar to the reported locations of the dwarfing genes Rht1 and Rht2, respectively. The Qph3A-2 with additive effect was not reported in previous linkage mapping studies. The total QTL effects detected for the plant height explained 85.04% of the phenotypic variation, with additive effects 46.07%, epistatic effects 19.89%, and QEs 19.09%. The results showed that both additive effects and epistatic effects were important genetic bases of wheat plant height, which were subjected to environmental modifications, and caused dramatic changes in phenotypic effects. The information obtained in this study will be useful for manipulating the QTLs for wheat plant height by molecular marker-assisted selection (MAS). | Kunpu Zhang Jichun Tian Liang Zhao Shanshan Wang | 2008 | Journal of Genetics and Genomics2008，35，2： | 36 |
| 11 | Thinopyrum ponticum and Th. intermedium: the promising source of resistance to fungal and viral diseases of wheat显示文摘Thinopyrum ponticum and Th. intermedium provide superior resistance against various diseases in wheat (Ttricum aestivum). Because of their readily crossing with wheat, many genes for disease resistance have been introduced from the wheatgrasses into wheat. Genes for resistance to leaf rust, stem rust, powdery mildew, Barley yellow dwarf virus, Wheat streak mosaic virus, and its vector, the wheat curl mite, have been transferred into wheat by producing chromosome translocations. These genes offer an opportunity to improve resistance of wheat to the diseases; some of them have been extensively used in protecting wheat from damage of the diseases. Moreover, new resistance to diseases is continuously detected in the progenies of wheat-Thinopyrum derivatives. The present article summaries characteri-zation and application of the genes for fungal and viral diseaseresistance derived from Th. ponticum and Th. intermedium. | Hongjie Li Xiaoming Wang | 2009 | Journal of Genetics and Genomics2009，36，9： | 35 |
| 12 | Activation of gibberellin 2-oxidase 6 decreases active gibberellin levels and creates a dominant semi-dwarf phenotype in rice (Oryza sativa L.)显示文摘Gibberellin (GA) 2-oxidase plays a key role in the GA catabolic pathway through 2β-hydroxylation.In the present study,we isolated a CaMV 35S-enhancer activation tagged mutant,H032.This mutant exhibited a dominant dwarf and GA-deficient phenotype,with a final stature that was less than half of its wild-type counterpart.The endogenous bioactive GAs are markedly decreased in the H032 mutant,and application of bioactive GAs (GA3 or GA4) can reverse the dwarf phenotype.The integrated T-DNA was detected 12.8 kb upstream of the OsGA2ox6 in the H032 genome by TAIL-PCR.An increased level of OsGA2ox6 mRNA was detected at a high level in the H032 mutant,which might be due to the enhancer role of the CaMV 35S promoter.RNAi and ectopic expression analysis of OsGA2ox6 indicated that the dwarf trait and the decreased levels of bioactive GAs in the H032 mutant were a result of the up-regulation of the OsGA2ox6 gene.BLASTP analysis revealed that OsGA2ox6 belongs to the class III of GA 2-oxidases,which is a novel type of GA2ox that uses C20-GAs (GA12 and/or GA53) as the substrates.Interestingly,we found that a GA biosynthesis inhibitor,paclobutrazol,positively regulated the OsGA2ox6 gene.Unlike the over-expression of OsGA2ox1,which led to a high rate of seed abortion,the H032 mutant retained normal flowering and seed production.These results indicate that OsGA2ox6 mainly affects plant stature,and the dominant dwarf trait of the H032 mutant can be used as an efficient dwarf resource in rice breeding. | Jian Huang Ding Tang Yi Shen Baoxiang Qin Lilan Hong Aiqing You Ming Li Xin Wang Hengxiu Yu Minghong Gu Zhukuan Cheng | 2010 | Journal of Genetics and Genomics2010，37，1： | 36 |
| 13 | Polymorphisms in PPARD,PPARG and APM1 associated with four types of Traditional Chinese Medicine constitutions显示文摘Based on the theory of constitution of Traditional Chinese Medicine (TCM), the human population is divided into nine constitutions including one balanced constitution (Normality) and eight unbalanced constitutions (Yang-deficiency, Yin-deficiency, Phlegm-wetness, Qi-deficiency, Wetness-heat, Blood stasis, Depressed constitution, and Inherited special constitution). Different constitutions have specific metabolic features and different susceptibility to certain diseases. However, whether a genetic basis accounts for such constitution classifica-tion is yet to be determined. Here we performed a genetic study to assess the association between genetic variations of metabolic genes in-cluding PPARD, PPARG and APM1 and the constitutions. A total of 233 individuals of the Han population in China were classified into four groups, Normality, Yang-deficiency, Yin-deficiency and Phlegm-wetness with whom 23 single nucleotide polymorphisms (SNPs) in the three genes were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Biased distri-bution of PPARD rs2267669 and rs2076167, APM1 rs7627128 and rs1063539 in Yang-deficiency, PPARG Pro12Ala in Yin-deficiency and PPARD rs2076167, APM1 rs266729 and rs7627128 in Phlegm-wetness were observed. The frequencies of Haplotype13 (Hap13) of PPARG in Yin-deficiency, Hap25 of APM1 in Yang-deficiency and Hap2 of PPARD and Hap14 of PPARG in Phlegm-wetness, were significantly different from those in Normality, suggesting those might be group-associated haplotypes. These results suggested that single SNP and hap-lotypes of PPARD, PPARG and APM1 may underlie the genetic basis of the constitutions classified in TCM. | Yanrui Wu Yina Cun Jing Dong Jingru Shao Shengjun Luo Shengjie Nie Haijing Yu Bingrong Zheng Qi Wang Chunjie Xiao | 2010 | Journal of Genetics and Genomics2010，37，6： | 36 |
| 14 | Identification and characterization of putative CIPK genes in maize显示文摘当在细胞内部的发信号的第二个送信人由发展、环境的暗示得到了,钙(Ca ) 起一个关键作用。Calcineurin 象 B 一样蛋白质(CBL ) 和他们的目标蛋白质,交往 CBL 蛋白质 kinases (CIPK ) 在植物响应压力作为一个关键调停 Ca2+ 的发信号网络出现了。生物信息的分析被用来识别 43 通常认为的 ZmCIPK (Zea mays CIPK ) 在玉米的染色体的基因生来的线 B73。基于基因结构,这些 ZmCIPKs 被划分成 intron 富有、 intron 差的组。种系发生的分析显示 ZmCIPK 家庭与 30 个成员的米饭 CIPK 家庭一起有一种高进化的关系。Microarray 数据和 RT-PCR 试金显示出基因 transcriptionally 回答了到不能生活的压力,和基因是的那 24, 31, 20 和 19 ZmCIPK 的那 ZmCIPK 由盐,干旱,热和冷压力起来调整分别地。在冷应力下面有在寒冷容忍的生来的线 B73 和寒冷敏感的生来的线 Mo17 之间的 ZmCIPKs 的不同表示模式。我们的调查结果将在玉米帮助 CIPK 的生物功能的进一步分子的解剖,并且提供新卓见进在植物表明网络的 CBLCIPK。 | Xifeng Chen Zhimin Gu Dedong Xin Liang Hao Chengjie Liu Ji Huang Bojun Ma Hongsheng Zhang | 2011 | Journal of Genetics and Genomics2011，38，2： | 40 |
| 15 | Molecular Cytogenetic Characterization of Wheat-Thinopyrum elongatum Addition,Substitution and Translocation Lines with a Novel Source of Resistance to Wheat Fusarium Head Blight显示文摘Thinopyrum elongatum(2n = 2x = 14,EE),a wild relative of wheat,has been suggested as a potentially novel source of resistance to several major wheat diseases including Fusarium Head Blight(FHB).In this study,a series of wheat(cv.Chinese Spring,CS) substitution and ditelosomic lines,including Th.elongatum additions,were assessed for TypeⅡresistance to FHB.Results indicated that the lines containing chromosome 7E of Th.elongatum gave a high level of resistance to FHB,wherein the infection did not spread beyond the inoculated floret.Furthermore,it was determined that the novel resistance gene(s) of 7E was located on the short-arm(7ES) based on sharp difference in FHB resistance between the two 7E ditelosomic lines for each arm.On the other hand,Th.elongatum chromosomes 5E and 6E likely contain gene(s) for susceptibility to FHB because the disease spreads rapidly within the inoculated spikes of these lines. Genomic in situ hybridization(GISH) analysis revealed that the alien chromosomes in the addition and substitution lines were intact,and the lines did not contain discernible genomic aberrations.GISH and multicolor-GISH analyses were further performed on three translocation lines that also showed high levels of resistance to FHB.Lines TA3499 and TA3695 were shown to contain one pair of wheat-Th. elongatum translocated chromosomes involving fragments of 7D plus a segment of the 7E,while line TA3493 was found to contain one pair of wheat-Th.elongatum translocated chromosomes involving the D- and A-genome chromosomes of wheat.Thus,this study has established that the short-arm of chromosome 7E of Th.elongatum harbors gene(s) highly resistant to the spreading of FHB,and chromatin of 7E introgressed into wheat chromosomes largely retained the resistance,implicating the feasibility of using these lines as novel material for breeding FHB-resistant wheat cultivars. | Shulan Fu Zhenling Lv Bao Qi Xiang Guo Jun Li Bao Liu Fangpu Han | 2012 | Journal of Genetics and Genomics2012，39，2： | 35 |
| 16 | The PI3K/AKT Pathway and Renal Cell Carcinoma显示文摘The phosphatidylinositol 3 kinase(PI3K)/AKT pathway is genetically targeted in more pathway components and in more tumor types than any other growth factor signaling pathway,and thus is frequently activated as a cancer driver.More importantly,the PI3K/AKT pathway is composed of multiple bifurcating and converging kinase cascades,providing many potential targets for cancer therapy.Renal cell carcinoma(RCC) is a high-risk and high-mortality cancer that is notoriously resistant to traditional chemotherapies or radiotherapies.The PI3K/AKT pathway is modestly mutated but highly activated in RCC,representing a promising drug target.Indeed,PI3 K pathway inhibitors of the rapalog family are approved for use in RCC.Recent large-scale integrated analyses of a large number of patients have provided a molecular basis for RCC,reiterating the critical role of the PI3K/AKT pathway in this cancer.In this review,we summarize the genetic alterations of the PI3K/AKT pathway in RCC as indicated in the latest large-scale genome sequencing data,as well as treatments for RCC that target the aberrant activated PI3K/AKT pathway. | Huifang Guo Peter German Shanshan Bai Sean Barnes Wei Guo Xiangjie Qi Hongxiang Lou Jiyong Liang Eric Jonasch Gordon B.Mills Zhiyong Ding | 2015 | Journal of Genetics and Genomics2015，42，7： | 39 |
| 17 | Fine mapping and candidate gene analysis of a green-revertible albino gene gra(t) in rice显示文摘Green-revertible albino is a novel type of chlorophyll deficiency in rice(Oryza sativa L.), which is helpful for further research in chlorophyll synthesis and chloroplast development to illuminate their molecular mechanism.In the previous study, we had reported a single recessive gene, gra(t), controlling this trait on the long arm of chromosome 2.In this paper, we mapped the gra(t) gene using 1,936 recessive individuals with albino phenotype in the F2 population derived from the cross between themo-photoperiod-sensitive genic male-sterile(T/PGMS) line Pei'ai 64S and the spontaneous mutant Qiufeng M.Eventually, it was located to a confined region of 42.4 kb flanked by two microsatellite markers RM2-97 and RM13553.Based on the annotation results of RiceGAAS system, 11 open reading frames(ORFs) were predicted in this region.Among them, ORF6 was the most possible gene related to chloroplast development, which encoded the chloroplast protein synthesis elongation factor Tu in rice.Therefore, we designated it as the candidate gene of gra(t).Sequence analysis indicated that only one base substitution C to T occurred in the coding region, which caused a missense mutation(Thr to Ile) in gra(t) mutant.These results are very valuable for further study on gra(t) gene. | Tao Chen Yadong Zhang Ling Zhao Zhen Zhu Jing Lin Suobing Zhang Cailin Wang | 2009 | Journal of Genetics and Genomics2009，36，2： | 31 |
| 18 | RLINl,encoding a putative coproporphyrinogen Ⅲ oxidase,is involved in lesion initiation in rice显示文摘Lesion mimic is necrotic lesions on plant leaf or stem in the absence of pathogenic infection,and its exact biological mechanism is varied.By a large-scale screening of our T-DNA mutant population,we identified a mutant rice lesion initiation 1(rlin1),which was controlled by a single nuclear recessive gene.Map-based cloning revealed that RLIN1 encoded a putative coproporphyrinogen Ⅲ oxidase in tetrapyrrole biosynthesis pathway.Sequencing results showed that a G to T substitution occurred in the second exon of RLIN1 and led to a missense mutation from Asp to Tyr.Ectopic expression of RLIN1 could rescue rlin1 lesion mimic phenotype.Histochemical analysis demonstrated that lesion formation in rlin1 was light-dependent accompanied by reactive oxygen species accumulated.These results suggest that tetrapyrrole participates in lesion formation in rice. | Changhui Sun Linchuan Liu Jiuyou Tang Aihong Lin Fantao Zhang Jun Fang Genfa Zhang Chengcai Chu | 2011 | Journal of Genetics and Genomics2011，38，1： | 31 |
| 19 | CRISPR/Cas9 and Genome Editing in Drosophila显示文摘Recent advances in our ability to design DNA binding factors with specificity for desired sequences have resulted in a revolution in genetic engineering, enabling directed changes to the genome to be made relatively easily. Traditional techniques for generating genetic mutations in most organisms have relied on selection from large pools of randomly induced mutations for those of particular interest, or time-consuming gene targeting by homologous recombination. Drosophila melanogaster has always been at the forefront of genetic analysis, and application of these new genome editing techniques to this organism will revolutionise our approach to performing analysis of gene function in the future. We discuss the recent techniques that apply the CRISPR/Cas9 system to Drosophila, highlight potential uses for this technology and speculate upon the future of genome engineering in this model organism. | Andrew R.Bassett Ji-Long Liu | 2014 | Journal of Genetics and Genomics2014，41，1： | 32 |
| 20 | 中国新疆野苹果[Malus sieversii(Lebed.) Roem.]群体遗传结构的SSR分析(英文)显示文摘以中国新疆伊犁地区的巩留县莫合镇库尔德宁、新源县交吾托海、霍城县大西沟和塔城地区的裕民县巴尔鲁克山4个种下居群的109个新疆野苹果实生株系为材料,利用8对苹果SSR引物进行群体遗传结构的研究。结果表明:8对SSR引物在4个居群中可平均扩增出16条带,其中巩留县居群多态性带数百分比最高为89.06%,各位点平均Nei基因多样度为0.257;4个群体共扩增出128个位点,在种级水平及巩留县、新源县、霍城县和裕民县4个居群水平多态性位点百分比分别为100%、88.28%、84.38%、87.50%、78.12%,种级水平Nei基因多样度(H=0.2619)和香农信息指数(I=0.4082)大于种下居群,4个种下居群Nei基因多样度和香农信息指数比较巩留县>霍城县>新源县>裕民县;巩留县居群和新源县居群遗传一致度最大,遗传距离最近;根据基因分化系数(GST=0.064)值,测得的基因流Nm为7.265。UPGMA聚类分析结果表明,巩留县和新源县居群遗传关系最近,霍城县居群次之,裕民县居群远离其他3个居群,巩留县、新源县、霍城县和裕民县4个居群是相对独立的群体,但同时存在部分基因交流。所有参数分析表明,巩留县遗传多样性最丰富,故在制定原位种质保护计划时应优先考虑巩留县居群。 | 张春雨 陈学森 何天明 刘晓丽 冯涛 苑兆和 | 2007 | Journal of Genetics and Genomics2007，34，10： | 30 |
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