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| 1 | Antibiotic sensitivity pattern of bacterial pathogens in the intensive care unit of Fatmawati Hospital,Indonesia显示文摘Objective:To evaluate the sensitivity pattern of bacterial pathogens in the intensive care unit(ICU) of a tertiary care of Falmawati Hospital Jakarta Indonesia.Methods:A cross sectional retrospective study of bacterial pathogen was carried out on a total of 722 patients that were admitted to the ICU of Fatmawati Hospital Jakarta Indonesia during January 2009 to March 2010. All bacteria were identified by standard microbiologic methods,and(heir antibiotic susceptibility testing was performed using disk diffusion method.Results:Specimens were collected from 385 patients who were given antimicrobial treatment,of which 249(64.68%) were cultured positive and 136(35.32%) were negative.The most predominant isolate was Pseudomonas aeruginosa(P.aeruginosa)(26.5%) followed by Klebsiella pneumoniae(K.pneumoniae)(15.3%) and Staphylococcus epidermidis(14.9%).P.aeruginosa isolates showed high rate of resistance to cephalexin(95.3%),cefotaxime(64.1%),and ceftriaxone(60.9%).Amikacin was the most effective(84.4%) antibiotic against P.aeruginosa followed by imipenem(81.2%),and meropenem(75.0%).K.pneumoniae showed resistance to cephalexin(86.5%),ceftriaxone(75.7%),ceftazidime(73.0%),cefpirome(73.0%) and cefotaxime(67.9%),respectively.Conclusions:Most bacteria isolated from ICU of Fatmawati Hospital Jakarta Indonesia were resistant to the third generation of cephalosporins,and quinolone antibiotics.Regular surveillance of antibiotic susceptibility pallerns is very important for setting orders to guide the clinician in choosing empirical or directed therapy of infected patients. | Maksum Radji Siti Fauziah Nurgani Aribinuko | 2011 | Asian Pacific Journal of Tropical Biomedicine2011，1，1： | 7 |
| 2 | Microbiological assessment of indoor air of a teaching hospital in Nigeria显示文摘Objective:To investigate the quality of indoor air of different wards and units of Olabisi Onabanjo University Teaching Hospital, Sagamu, to ascertain their contribution to infection rate in the hospital.Methods:The microbial quality of indoor air of nine wards/units of Olabisi Onabanjo University Teaching Hospital, Sagamu, Nigeria was conducted. Sedimentation technique using open Petri-dishes containing different culture media was employed and samplings were done twice daily, one in the morning shortly after cleaning and before influx of people/patients into the wards/units and the other in the evening when a lot of activities would have taken place in these wards. Isolates were identified according to standard methods.Results:Results showed that there was a statistically significant difference(氈2= 6.0167) in the bacteria population of the different sampling time whereas it was not so for fungi population(氈2= 0.2857). Male medical ward(MMW) and male surgical general(MSG) recorded the highest bacterial and fungal growth while the operating theatre(OT) was almost free of microbial burden. The bacteria isolates were Staphylococcus aureus, Klebsiellasp., Bacillus cereus, Bacillus subtilis, Streptococcus pyogenes andSerratia marscenceswhile the fungi isolates includedAspergillus flavus, Penicilliumsp.,Fusariumsp.,Candida albicansandAlternariasp.Staphylococcus aureuswas the predominantly isolated bacterium whilePenicilliumsp. was the most isolated fungus.Conclusions:Though most of the microbial isolates were potential and or opportunistic pathogens, there was no correlation between the isolates in this study and the surveillance report of nosocomial infection during the period of study, hence the contribution of the indoor air cannot be established. From the reduction noticed in the morning samples, stringent measures such as proper disinfection and regular cleaning, restriction of patient relatives' movement in and out of the wards/units need to be enforced so as to improve the quality of indoor air of our hospital wards/units. | Awosika SA Olajubu FA Amusa NA | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，6： | 6 |
| 3 | Antibacterial activity of Lawsonia inermis Linn(Henna) against Pseudomonas aeruginosa显示文摘Objective:To investigate the antibacterial activity of henna(Lawsonia inermis Linn) obtained from different regions of Oman against a wide array of micro-organisms.Methods:fresh henna samples were obtained from different regions of Oman as leaves and seeds,100 g fresh and dry leaves and SO g of fresh and dry seeds were separately soaked in 500 mL of ethanol for three days,respectively,with frequent agitation.The mixture was filtered,and the crude extract was collected.The crude extract was then heated,at 48 ℃ in a water bath to evaporate its liquid content.The dry crude henna extract was then tested for its antibacterial activity using well-diffusion antibiotic susceptibility technique.Henna extracts were investigated for their antibacterial activity at different concentrations against a wide array of different micro-organisms including a laboratory standard bacterial strain of Pseudomonas aeruginosa(NCTC 10662)(A aeruginosa) and eleven fresh clinical isolates of P.aeruginosa obtained from patients attending the Sultan Qaboos University Hospital(SQUH).2-Hydroxy-p-Nathoqinone-Tech(2-HPNT, MW=174.16,C_(10)H_40_3) was included as control(at 50%concentration) along with the henna samples tested.Results:Henna samples demonstrated antibacterial activity against all isolates but the highest susceptibility was against P.aeruginosa with henna samples obtained from Al-sharqyia region.Conclusions:Omani henna from Al-sharqyia region demonstrates high in vitro anti-P. aeruginosa activity compared with many henna samples from different regions of Oman. | Habbal O Hasson SS El-Hag AH Al-Mahrooqi Z Al-Hashmi N Al-Bimani Z MS Al-Balushi Al-Jabri AA | 2011 | Asian Pacific Journal of Tropical Biomedicine2011，1，3： | 3 |
| 4 | Fox- and raccoon-dog–associated rabies outbreaks in northern China显示文摘Dear Editor,Rabies is a generally fatal disease caused by the rabies virus(RABV),and is transmitted mainly by Carnivora and Chiroptera(Fooks A R,et al.,2014;Tao X,et al.,2013).In China,stray dogs and some wild animals(e.g.,Chinese ferret badgers,foxes,and raccoon dogs)are the principal reservoirs for RABV(Hu R L,et al.,2009).Historically,rabies in wild foxes and raccoon dogs(Nyctereutes procyonoides)was recorded in the | Ye Liu Shoufeng Zhang Jinghui Zhao Fei Zhang Nan Li Hai Lian Wurengege Shiyu Guo Rongliang Hu | 2014 | Virologica Sinica2014，29，5： | 3 |
| 5 | Assessment of in vitro sensitivity of Plasmodium vivax fresh isolates显示文摘Objective:To compare the applicability of the SYBK Grcen-Ⅰ assay with the standard schizont maturalion assay,for determination of sensitivity of Plasmodium vivax(P.vivax) to chloroquine and a new antifolale WR 99210.Methods:The study was conducted at Mae Tao Clinic for migrant workers,Tak Province during April 2009 to July 2010.A total of 64 blood samples(1 mL blood collected into sodium heparinized plastic tube) were collected from patients with monoinfection with P.vivax malaria prior to treatment with standard regimen of a 3-day chloroquine. In vitro sensitivity of P.vivax isolates was evaluated by schizont maturation inhibition and SYBR Green-Ⅰ assays.Results:A total of 30 out of 64 blood samples collected from patients with P.vivax malaria were successfully analyzed using both the microscopic schizont maturation inhibition and SYBR Green-I assays.The failure rates of the schizont maturation inhibition assay(50%) and the SYBR Green-I assay(54%) were similar(P=0.51).The median IC_(10)s,IC_(50)s and IC_(90)s of both chloroquine and WR99210 were not significantly different from the clinical isolates of P.vivax tested.Based on the cut-off of 100 nM,the prevalences of chloroquine resistance determined by schizont maturation inhibition and SYBR Green-I assays were 19 and 11 isolates,respectively.The strength of agreement between the two methods was very poor for both chloroquine and WR992I0.Conclusions:On the basis of this condition and its superior sensitivity,the microscopic method appears better than the SYUK Green-I Green assay for assessing in vitro sensitivity of fresh P.vivax isolates to antimalarial drugs. | Poonuch Muhamad Wanna Chacharoenkul Kanchana Rungsihirunrat Ronnatrai Ruengweerayut Kesara NaBangchang | 2011 | Asian Pacific Journal of Tropical Biomedicine2011，1，1： | 2 |
| 6 | Positional cloning of PmCH1357 reveals the origin and allelic variation of the Pm2 gene for powdery mildew resistance in wheat显示文摘Powdery mildew, caused by the biotrophic fungus Blumeria graminis f. sp. tritici(Bgt), is a prevalent disease in common wheat(Triticum aestivum L.) and causes serious yield losses worldwide. We used a map-based approach to clone the major broad-spectrum powdery mildew resistance gene Pm CH1357 from wheat breeding line CH1357. Pm CH1357 was mapped to a 526 kb region containing only Traes CS5 D01 G044600. The Traes CS5 D01 G044600 sequence of the susceptibility allele in Taichung 29(TC29) was identical to that in Chinese Spring, whereas the sequence of the resistance allele in CH1357 was identical to Pm2a previously cloned from the germplasm Ulka/*8Cc. The susceptibility allele in TC29 contained a 7 bp deletion in exon 1, resulting in loss of 856 of the 1277 amino acids in the predicted nucleotide-binding domain leucine-rich repeat containing Pm2a protein.Pm CH1357/Pm2a sequence was also isolated from the Chinese wheat landraces and cultivars that were previously reported to possess the resistance gene Pm2b, Pm2c,PmLX66, or PmND399. The Pm CH1357/Pm2a resistance allele was present in 10 of 495 accessions in core germplasm and contemporary cultivars from China and the USA. A newly developed diagnostic marker for the 7 bp In Del in the resistance gene can be used to eliminate the susceptibility allele in wheat breeding programs. | Fang Chen Haiyan Jia Xiaojun Zhang Linyi Qiao Xin Li Jun Zheng Huijuan Guo Carol Powers Liuling Yan Zhijian Chang | 2019 | The Crop Journal2019，7，6： | 2 |
| 7 | Succinylated whey protein isolate as a sustained-release excipient of puerarin derivative oral tablets:Preparation,optimization and pharmacokinetics显示文摘This work was done to investigate succinylated commercial whey protein isolate(S-WPI)as an oral sustained-release delivery carrier for puerarin 5(PR-5). The succinylation condi-tions were established for S-WPIs by optimization of single factor study and Box–Beehnkendesign. The effect of succinylation degree on S-WPIs solubility was evaluated. Physicochem-ical properties of S-WPIs dried by different three methods on their flow ability, particle size,morphology and in vitro release behavior were studied. After preparing PR-5 sustained re-lease protein tablets with S-WPIs as the carrier by direct powder compression method, thedrug release were studied in vitro and the oral pharmacokinetics and bioavailability wasevaluated using in vivo dog model. It was observed that concentration of substrate has asignificant effect on succinylation. Release behavior in vitro showed spry dried S-WPIs with100% succinylation rate and 30% drug loading would be applied to the preparation of PR-5 sustained-release protein tablets based on the swelling mechanism(protein loss). Comparedwith PR-5 conventional tablet with oral administration, T max value of PR-5 sustained-releaseprotein tablets was approximately 1.58 fold greater than those of the conventional tablets asfurther evidenced by the significantly prolonged MRT and T 1/2. The findings demonstratedthat spray-dried S-WPIs has potential as a promising functional excipient for the design of PR-5 oral sustained-release tablets which can fully improve sustained-release effect and oral bioavailability. | Rui Zhang Yu Zhang Yue Wu Jun Liu Tiantian Ye Shujun Wang | 2018 | Asian Journal of Pharmaceutical Sciences2018，13，4： | 2 |
| 8 | Degradation of Phenolic Compounds in Coal Gasification Wastewater by Biofilm Reactor with Isolated Klebsiella sp.显示文摘This study was conducted to evaluate the degradation of phenolic compounds by one strain isolated from coal gasification wastewater( CGW). 16S rRNA gene sequences homology and phylogenetic analysis showed that the isolate is belonged to the genus Klebsiella sp. The effect of different phenolic compounds on the isolate was investigated by determining OD600and phenoloxidase activity,of which the results showed that the isolate can utilize phenol,4-methyl phenol,3,5-dimethyl phenol and resorcinol as carbon resources. The biofilm reactor( formed by the isolate) can resist the influent concentration of phenolic compounds as high as750 mg /L when fed with synthetic CGW and incubated at optimum conditions. The capacity of improving the biodegradability of CGW through degrading phenolic compounds was testified with fed the biofilm reactor with real CGW. Thus,it might be an effective strain for bioaugmentation of CGW treatment. | Fang Fang Hong-Jun Han Chun-Yan Xu Qian Zhao Ling-Han Zhang | 2014 | Journal of Harbin Institute of Technology(New Series)2014，21，3： | 2 |
| 9 | Preparation and Properties of Plywood Adhesives with Soy Protein Isolate Modified by SDS显示文摘The effects of mass concentration,the reaction temperature and the reaction time of soy protein isolate(SPI) and sodium dodecyl sulfate (SDS) on the adhesive strength of the modified SPI adhesives has been studied through orthogonal experiments.And the adhesive mechanism of the modified SPI adhesives was discussed.The results show that the optimum formula conditions are as follows:mass concentration of SPI was 14%,that of SDS 1%,reaction temperature 35℃and the reaction time 3 h.Under the optimum conditions, the dry adhesive strength of the modified SPI adhesives was 1.82 MPa,the wet adhesive strength was 0.82 MPa,the viscosity was 5.8 Pa·s,the solid content was 12.96%.After SDS was added into SPI, the composites of SDS-SPI were formed,the internal hydrophobic groups among the SPI molecule structure were turned out and the water resistance of the modified SPI adhesives was enhanced with an increase of the modification time.When the concentration of SDS was over a defined value,the disulfide bond was broken and the modification effect of SDS went bad. | Li Na Xie Jianjun Zeng Nian Ding Chu Ran Delong | 2012 | Chinese Forestry Science and Technology2012，11，3： | 1 |
| 10 | NEW ANTITUMOR GLYCOCYCLOHEXAPEPTIDE FROM RUBIA YUNNANENSIS显示文摘A new antitumor glycocyclohexapcptide,RY-III,was isolated from Rubia yunnanensis.The structure of RY-III was elucidated by means of spectral methods. | Min HE Cheng ZOU Xiao Jiang HAO Jun ZHOU Laboratory of Phytochemistry.Kunming Institute of Botany.Academia Sinica.Kunming,650204 | 1993 | Chinese Chemical Letters1993，4，12： | 1 |
| 11 | PREPARATION AND CHARACTERIZATION OF SOY PROTEIN ISOLATE(SPI)/MONTMORILLONITE(MMT) BIONANOCOMPOSITES显示文摘The bionanocomposites of soy protein isolate(SPI)/montmorillonite(MMT) have been prepared successfully via simple melt mixing,in which MMT was used as nanofiller and glycerol was used as plasticizer.Their structures and properties were characterized with X-ray diffraction(XRD),differential scanning calorimetry(DSC),scanning electron microscopy(SEM),thermogravimetric analysis and tensile testing.XRD、TEM and SEM results indicated that the MMT layers could be easily intercalated by the SPI matrix even by si... | 傅强 | 2009 | Chinese Journal of Polymer Science2009，27，6： | 1 |
| 12 | DISTRIBUTION AND CHARACTERIZATION OF NON-VOLATILE CHEMICAL MUTAGENS IN SEDIMRNTS OF AN INDUSTRIALLY IMPACTED RIVER显示文摘A coupled bioassay/analytical fractionation approachwas used to isolate genotoxic contaminants from riversediments collected at points upstream,adjacent to,and downstream from centers of major industrial andmunicipal activities.Sediments were milled withsodium sulfate and sequentially extracted with anonpolar to polar solvent series;extracts | M.Wilson Tabor Marcus C.Waldron Alan R.White | 1991 | 癌变.畸变.突变1991，3，S1： | 0 |
| 13 | ISOLATION AND STRUCTURE OF A NATURAL METALLIC COMPOUND—ZINCPOLYANEMINE显示文摘The Chinese medicinal plant Polyalthia nemoralis A. DC. (Anonaceae) grows in the south of Guangdong Province and Hainan Island. Its roots are often used by the natives of Hainan as a traditional cure for malaria and hepatitis. Since our attempt to isolate the effective component of the plant began in 1972 we have obtained | 徐炳祥 韩公羽 王肖鹏 刘明珠 许学余 孟利宁 陈仲良 朱大元 | 1980 | Chinese Science Bulletin1980，25，5： | 0 |
| 14 | A NEW METHOD OF ISOLATING KUPFFER CELLS FROM BIOPSY TISSUE OF RAT LIVER显示文摘The isolation of a high yield and purity of Kupffer cells has been reported in detail.1 This paper reports into the research about isolation Kupffer cells from biopsy tissue of liver. This method includes 5 important steps: (1) take fresh liver tissue, and mince with scissors. (2) spin at low speed to wash off red blood cells. (3) digest in collagenase for suitable time. (4) isolate Kupffer cells on a percoll density gradient. (5) cell charaterization was observed by N.S.E stain and peroxidatic activity with lumino-meter measurement and phagocytosis with latex beads.2. | 张力健 | 1990 | Chinese Journal of Cancer Research1990，2，3： | 0 |
| 15 | Genetic modification of T cell clones for therapy of human viral and malignant diseases显示文摘Our laboratory has pursued T cell therapy withantigen-specific T cell clones as means to treat humandisease. Our studies in the prevention of CMV | Philip Greenberg C.Yee M.Gilbert B.Nelson J.Lord A.Sing M.Kalos S.Riddell | 1997 | 中国实验血液学杂志1997，5，3： | 0 |
| 16 | ISOLATION, CULTIVATION AND INFECTIVITY OF ACTINORHIZAL ENDOPHYTE OF Casuarina equisetifolia显示文摘Since actinorhizal endophyte was first successfully isolated in 1978, various strains of Frankia have been isolated from nodules of Comptonia, Alnus, Elaeagnus, Hippophae, Myrica, etc. by using different techniques. But still many other attempts to isolate Fran- | 蒋建德 朱宝琴 | 1985 | Chinese Science Bulletin1985，30，6： | 0 |
| 17 | Study on Extraction Separation of ^(142)Ba-^(142)La With HDEHP显示文摘In order to accurately measure the decay data of short-life nuclide 142La, it is necessary to isolate and prepare radiochemical pure 142La solution from fission products. For the aim, the plan is made for isolating Ba from fission products first, and then isolating La that grows from Ba. So the separation of La-Ba becomes the key for solving above problem.A convenient method that realizes the separation of high-valent ionic La3+and low-valent | CUI An-zhi, DING You-qian, YANG Zhi-hong, YU Wei-xiang, ZHONG Qi-ping, Guo Jing-ru, ZHANG Sheng-dong, LI Da-ming | 2001 | Annual Report of China Institute of Atomic Energy2001，，0： | 0 |
| 18 | Identification of human genomic DNA from suppressed metastatic phenotype cells of mouse lung adenocarcinoma显示文摘Objectives:To isolate tumor metastaticsuppressing genes or relating human DNA sequencesand to study the molecular biological regulatingmechanism of tumor metastasis. Methods: HumanDNA fragments were amplified by Inter Alu PCRtechnique from normal human genomic DNAtransfected mouse tumor cell clones of which themetastatic phenotype had been suppressed. Thehuman origin of the amplified DNA was furtherconfirmed by PCR in situ hybridization. One | Xueming Ge, Yinglin Lu, Shengfa Fu, Kun Chen, Wenhong Fan, Shuang LiuDepartment of Molecular Pathologv, Beijing Institute of Basic Medical Sciences, Beijing 100850 | 1997 | 中国实验血液学杂志1997，5，3： | 0 |
| 19 | A STUDY OF CUTTING PROPAGATION OF RAMIE SHOOT显示文摘Underground stems of ramie are usually used as materials for vegetative propagation. The extention of good varieties is limited owing to its low propagation coefficient. Especially, it is difficult to isolate and propagate | 管和 李人圭 | 1984 | Chinese Science Bulletin1984，29，5： | 0 |
| 20 | Correlation of Inhibitor Proteinase in Varieties and Lines of Cotton(Gossypium hirsutum L.) to Different Geographic Population of Verticillium dathliae Klebahn显示文摘Breeding for wilt resistance and its theoretical basis are primarily responsible for increases in cotton yield and fiber quality. Breeding for immunity is the most efficient method in our struggle with infectious diseases. | KIM Robert AMANTURDIEV Alisher MEJLUMYAN Larisa BABAYEV Yashen KIM Michael | 2008 | 棉花学报2008，20，S1： | 0 |
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