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| 1 | Genetic diversity of Plasmodium falciparum isolates from naturally infected children in north-central Nigeria using the merozoite surface protein-2 as molecular marker显示文摘Objective: To characterize the genetic diversity of Plasmodium falciparum ( P. falciparum ) field isolates in children from Lafia, North-central Nigeria, using the highly polymorphic P. falciparum merozoite surface protein 2 (MSP-2) gene as molecular marker. Methods: Three hundred and twenty children were enrolled into the study between 2005 and 2006. These included 140 children who presented with uncomplicated malaria at the Dalhatu Araf Specialist Hospital, Lafia and another 180 children from the study area with asymptomatic infection. DNA was extracted from blood spot on filter paper and MSP-2 genes were genotyped using allele-specific nested PCR in order to analyze the genetic diversity of parasite isolates. Results: A total of 31 and 34 distinct MSP-2 alleles were identified in the asymptomatic and uncomplicated malaria groups respectively. No difference was found between the multiplicity of infection in the asymptomatic group and that of the uncomplicated malaria group ( P >0.05). However, isolates of the FC27 allele type were dominant in the asymptomatic group whereas isolates of the 3D7 allele type were dominant in the uncomplicated malaria group. Conclusions: This study showed a high genetic diversity of P. falciparum isolates in North-central Nigeria and is comparable to reports from similar areas with high malaria transmission intensity. | Segun Isaac Oyedeji Henrietta Oluwatoyin Awobode Chiaka Anumudu Jrgen Kun | 2013 | Asian Pacific Journal of Tropical Medicine2013，6，8： | 0 |
| 2 | Effect of insulin on Blattela germanica Linnacus显示文摘Objective:To determine the sensitivity of Blattela germanica L(B.germanica L) to differenct doses of insulin.Methods:B.germanica were reared in laboratory conditions at(25±2) ℃ and(50±5)%relative humidity(RH),and exposure period of 12:12 L/D.Different concentrations,viz.5,10,15,20 and 25 μ of insulin N,R,(N+R) were prepared and injected to 10 treated cockroaches with another 10 cockroaches which were injected with normal saline as control group.Results:Insulin N with a dose of 20 μ caused more than 70%mortality of B.germanica in this study.There was a significant difference between 20 μ of insulin N with other doses of 5,10,15 and 25 μ,and its comparison with other forms of medication also showed obvious difference(P<0.05).Conclusions:It can be concluded that effective drug doses of insulin which can be used as posion bait or gel against German cockroaches could be utilized in the control of B.germanica in the future field studies. | Abolghasemi E Moosa Kazem SH Abolhasani M Davoudi M | 2011 | Asian Pacific Journal of Tropical Biomedicine2011，1，4： | 0 |
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