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3篇 您的检索式:作者名="Poonuch Muhamad"
    题名 作者 年代 出处 被引量
1Assessment of in vitro sensitivity of Plasmodium vivax fresh isolates显示文摘Objective:To compare the applicability of the SYBK Grcen-Ⅰ assay with the standard schizont maturalion assay,for determination of sensitivity of Plasmodium vivax(P.vivax) to chloroquine and a new antifolale WR 99210.Methods:The study was conducted at Mae Tao Clinic for migrant workers,Tak Province during April 2009 to July 2010.A total of 64 blood samples(1 mL blood collected into sodium heparinized plastic tube) were collected from patients with monoinfection with P.vivax malaria prior to treatment with standard regimen of a 3-day chloroquine. In vitro sensitivity of P.vivax isolates was evaluated by schizont maturation inhibition and SYBR Green-Ⅰ assays.Results:A total of 30 out of 64 blood samples collected from patients with P.vivax malaria were successfully analyzed using both the microscopic schizont maturation inhibition and SYBR Green-I assays.The failure rates of the schizont maturation inhibition assay(50%) and the SYBR Green-I assay(54%) were similar(P=0.51).The median IC_(10)s,IC_(50)s and IC_(90)s of both chloroquine and WR99210 were not significantly different from the clinical isolates of P.vivax tested.Based on the cut-off of 100 nM,the prevalences of chloroquine resistance determined by schizont maturation inhibition and SYBR Green-I assays were 19 and 11 isolates,respectively.The strength of agreement between the two methods was very poor for both chloroquine and WR992I0.Conclusions:On the basis of this condition and its superior sensitivity,the microscopic method appears better than the SYUK Green-I Green assay for assessing in vitro sensitivity of fresh P.vivax isolates to antimalarial drugs.Poonuch Muhamad Wanna Chacharoenkul Kanchana Rungsihirunrat Ronnatrai Ruengweerayut Kesara NaBangchang 2011Asian Pacific Journal of Tropical Biomedicine2011,1,1:2
2SYBR GREEN I AND TAQMAN QUANTITATIVE REAL-TIME POLYMERASE CHAIN REACTION METHODS FOR THE DETERMINATION OF AMPLIFICATION OF PLASMODIUM FALCIPARUM MULTIDRUG RESISTANCE-1 GENE (PFMDR1)显示文摘Muhamad Poonuch Chaijaroenkul Wanna Congpuong Kanungnit Na-Bangchang Kesara 2011EN2011,,5:1
3Molecular analysis of pfatp6 and pfmdr1 polymorphisms and their association with in vitro sensitivity in Plasmodium falciparum isolates from the Thai-Myanmar border显示文摘Papichaya Phompradit Raewadee Wisedpanichkij Poonuch Muhamad Wanna Chaijaroenkul Kesara Na-Bangchang 2011Acta Tropica2011,,1:1
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