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| 1 | Regulatory effects of antitumor agent matrine on FOXO and PI3K-AKT pathway in castration-resistant prostate cancer cells显示文摘We previously demonstrated that matrine could inhibit the proliferating, migrating, as well as invading processes of both PC-3 and DU145 cells. However, the underlying molecular mechanisms have not yet been clearly defined. In this study, using various techniques such as high throughput sequencing technology, bioinformatics, quantitative real-time PCR, and immunoblot analysis,we aimed to understand whether matrine serves as a novel regulator of FOXO and PI3K-AKT signaling pathway. DU145 and PC-3 cell lines were cultured for 24 h in vitro. Cells were treated with either matrine or control serum for 48 h, followed by extraction of total RNA. The RNA was sequenced using HiSeq 2500 high-throughput sequencing platform (Illumina). A gene library was established and quality analysis of read data carried out. Integrated database from the website DAVID was used to analyze Gene Ontology (GO), and Kyoto encyclopedia of genes and genomes (KEGG) pathway of differential genes was used for pathway analysis, screening for fold differences of more than two times. The FOXO and PI3K-AKT signaling pathways were screened, and expression levels of mRNA and core protein detected by real-time PCR and immunoblotting, respectively. High throughput sequencing and GO analysis revealed that differentially expressed genes before and after treatment played an important role in cell metabolic process, growth process, anatomical structure formation, cellular component organization, and biological regulation. KEGG signal pathway analysis revealed that FOXO and PI3K-AKT signal pathways had a significant difference between before and after matrine-treated androgen-independent prostate cancer cells PC-3 and DU145. Real-time PCR showed that matrine treatment led to a significant increase in the expression levels of FOXO1A, FOXO3A, FOXO4, and FOXO6 in DU145 and PC-3 cells (P<0.01 or P<0.05), whereas the PI3K expression levels decreased (P<0.01). Similarly, immunoblotting revealed a significant increase (P<0.05) in the expression levels of FOXO1A FOXO3A, FOXO4, and FOXO6 in both PC-3 and DU145 cells, whereas PI3K expression levels decreased (P<0.05). Matrine had a broad regulating effect on the mRNA expression profiles of both PC-3 and DU145 cells. Matrine may inhibit cell proliferation, migration, as well as invasion, and induce apoptosis in both PC-3 and DU145 cells through FOXO and PI3K-AKT signaling pathways. Matrine could therefore be used as a complementary drug to present chemotherapeutic agents, for treating androgen-independent prostate cancer. | Qi Li HaiH uang Zheng He Yi Sun Yufeng Tang Xiaohong Shang Chengbin Wang | 2018 | Science China(Life Sciences)2018，61，5： | 9 |
| 2 | Extracting DC bus current information for optimal phase correction and current ripple in sensorless brushless DC motor drive显示文摘Brushless DC motor(BLDCM) sensorless driving technology is becoming increasingly established. However, optimal phase correction still relies on complex calculations or algorithms. In finding the correct commutation point, the problem of phase lag is introduced. In this paper, we extract DC bus current information for auto-calibrating the phase shift to obtain the correct commutation point and optimize the control of BLDC sensorless driving. As we capture only DC bus current information, the original shunt resistor is used in the BLDCM driver and there is no need to add further current sensor components. Software processing using only simple arithmetic operations successfully accomplishes the phase correction. Experimental results show that the proposed method can operate accurately and stably at low or high speed, with light or heavy load, and is suitable for practical applications. This approach will not increase cost but will achieve the best performance/cost ratio and meet market expectations. | Zu-sheng HO Chii-maw UANG Ping-chieh WANG | 2014 | Journal of Zhejiang University-Science C(Computers and Electronics)2014，15，4： | 3 |
| 3 | Single Cr atom catalytic growth of graphene显示文摘 | Huy Q. Ta Uang Zhao Wanjian Yin Darius Pohl Bemd Rellinghaus Thomas Gemming Barbara Trzebicka Justinas Palisaitis Gao Jing Per O. A. Persson Zhongfan Liu Alicja Bachmatiuk Mark H. Rummeli | 2018 | Nano Research2018，11，5： | 3 |
| 4 | Posttensioned Energy Dissipating Connections for Moment-Resisting Steel Frames显示文摘 | Constantin Christopoulos Andre Filiatrault Chia-Ming Uang Bryan Folz | 2002 | Journal of Structural Engineering2002，，9： | 2 |
| 5 | Lyapunov stability and generalized invariance principle for nonconvex differential inclusions显示文摘这份报纸学习 nonconvex 的一个班的系统稳定性问题微分包括。起初,基本稳定性结果被优点获得局部地, Lipschitz 连续 Lyapunov 工作。而且,一个概括不变性原则和相关吸引力条件被建议并且证明了由于凸状的缺席克服技术困难。在技术分析,新奇珍视集合的衍生物被建议处理 nonsmooth 系统和 nonsmooth Lyapunov 功能。另外,获得的结果与在有常规 Lyapunov 功能的凸的微分包括的情况中的存在的一致。最后,解说性的例子被给显示出方法的有效性。 | Shu UANG Xianlin ZENG Yiguang HONG | 2016 | Control Theory and Technology2016，14，2： | 2 |
| 6 | Cyclic response and design recommendations of reduced beam section moment connections with deep columns 显示文摘 | Brandon C Uang Chia-Ming | 2002 | Journal of Structural Engineering2002，128，4： | 1 |
| 7 | Fuzzy Tracking Control Design for Nonlinear Dynamic Systems via T - S fuzzy Model 显示文摘 | Tseng C S Chen B S Uang H | 2001 | IEEE Transactions on Fuzzy Systems2001，9，3： | 1 |
| 8 | Heterogeneous Photopolymerization of Methyl Methacrylate Initiated by Small ZnO Parti- cles 显示文摘 | H uang Z Y Barber T Mills G | 1994 | J Phys Chem1994，98，12： | 1 |
| 9 | Robust Adaptive Optimal Tracking Design for Uncertain Missile Systems : a Fuzzy Approach显示文摘 | Uang Hueyjian Chen Borsen | 2002 | Fuzzy Sects and Systems2002，126，： | 1 |
| 10 | Activation of peroxisome prolif- erator-activated receptor-7 coactivator is ameliorates mitochon- drial dysfunction and protects podocytes from aldosterone-indueed injury显示文摘 | Yuan Y H uang S Wang W | 2012 | Kidney Int2012，82，7： | 1 |
| 11 | Clinical and virological characteristics of hepatitis B virus subgenotypes Ba,C1,and C2 in China显示文摘 | Wang Z Tanaka Y H uang Y | | 0，，05： | 1 |
| 12 | Mechanism of chronic aristolo- chic acid nephropathy= role of Smad3显示文摘 | Zhou L Fu P H uang XR | 2010 | Am J Physiol Renal Physiol2010，298，4： | 1 |
| 13 | Fuzzy tracking control design for nonlinear dynamic systems via T-S fuzzy model 显示文摘 | Tseng C S Chen B S Uang H J | 2001 | IEEE Trans on Fuzzy Systems2001，9，3： | 1 |
| 14 | Comparision of germination strategies of Artemisia ordosica with its two congeners from deserts of China and Israel显示文摘 | Uang Z Y Gutterman Y | 2000 | Acta Bot Sin2000，42，1： | 1 |
| 15 | Tuning ionic liquids for high gas solubility and reversible gas sorption显示文摘 | H UANG J IISAGER A R BERG R W | 2008 | Molecular Catalysis2008，279，2： | 1 |
| 16 | Fuzzy Tracking Control Design for Nonlinear Dynamic Systems Via T-S Fuzzy Model显示文摘 | Tseng C S Chen B S Uang H J | 2001 | IEEE Trans on Fuzzy Systems2001，9，3： | 1 |
| 17 | Immunogenicity and efficacy of three recombinant subunit PasteureZla multoci- da toxin vaccines against progressive atrophic rhinitis in pigs 显示文摘 | LIAO C M H UANG C J HSUAN S L | 2006 | Vaccine2006，24，1： | 1 |
| 18 | Analysis of a viral infection model with delayed immune response显示文摘 | XIE Qizhi H UANG Dongwei ZHANG Shuangde | 2010 | Applied Mathematical ModeUing2010，34，9： | 1 |
| 19 | Rational syntheses, characterization, crystal structure, and replacement reactions of coordinated water molecules of (M = Cd, Co, Cu, Fe, Mn, Ni or Zn) 显示文摘 | BI L H H UANG R D PENG J | 2001 | J Chem Soc Dalton Trans2001，，： | 1 |
| 20 | Cytokine-stimulated GTP cyclohydrolase I expression in endothelial cells requires coordinated activation of nuclear faetor-kappaB and Statl / StatS显示文摘 | H uang A Zhang YY Chen K | 2005 | Circ Res2005，96，2： | 1 |
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