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| 1 | Hepatogenic differentiation of human mesenchymal stem cells from adipose tissue in comparison with bone marrow mesenchymal stem cells显示文摘AIM: To investigate and compare the hepatogenic transdifferentiation of adipose tissue-derived stem cells (ADSC) and bone marrow-derived mesenchymal stem cells (BMSC) in vitro. Transdifferentiation of BMSC into hepatic cells in vivo has been described. Adipose tissue represents an accessible source of ADSC, with similar characteristics to BMSC.METHODS: BMSCs were obtained from patients under-going total hip arthroplasty and ADSC from human adi-pose tissue obtained from lipectomy. Cells were grown in medium containing 15% human serum. Cultures were serum deprived for 2 d before cultivating under similar pro-hepatogenic conditions to those of liver develop-ment using a 2-step protocol with sequential addition of growth factors, cytokines and hormones. Hepatic differ-entiation was RT-PCR-assessed and liver-marker genes were immunohistochemically analysed. RESULTS: BMSC and ADSC exhibited a fibroblastic mor-phology that changed to a polygonal shape when cells differentiated. Expression of stem cell marker Thy1 de-creased in differentiated ADSC and BMSC. However, the expression of the hepatic markers, albumin and CYPs increased to a similar extent in differentiated BMSC and ADSC. Hepatic gene activation could be attributed to in-creased liver-enriched transcription factors (C/EBPβ and HNF4α), as demonstrated by adenoviral expression vec-tors.CONCLUSION: Mesenchymal stem cells can be induced to hepatogenic transdifferentiation in vitro. ADSCs have a similar hepatogenic differentiation potential to BMSC, but a longer culture period and higher proliferation ca-pacity. Therefore, adipose tissue may be an ideal source of large amounts of autologous stem cells, and may become an alternative for hepatocyte regeneration, liver cell transplantation or preclinical drug testing. | Raquel Taléns-Visconti Ana Bonora Ramiro Jover Vicente Mirabet Francisco Carbonell José Vicente Castell María José Gómez-Lechón | 2006 | World Journal of Gastroenterology2006，12，36： | 35 |
| 2 | Intraductal endoscopic radiofrequency ablation for the treatment of hilar non-resectable malignant bile duct obstruction显示文摘AIM: To evaluate the safety and technical success of endoscopic radiofrequency ablation(RFA) for palliative treatment of malignant hilar bile duct obstruction. METHODS: In this study, a recently CE and FDA-approved endoscopic RFA catheter was first tested in an ex vivo pig liver model to study the effect of electrosurgical variables on the extent of the area of induced necrosis. Subsequently, a retrospective analysis was conducted of all patients treated with endoscopic RFA for malignant biliary obstruction at our center between February 2012 and April 2013. All patients received an additional plastic stent implantation into the biliary tree following RFA. RESULTS: In the pig model, ablation time of 60-90 seconds using the bipolar soft coagulation mode at 8-10 watts with an effect of 8 was found to be the most feasible setting. Twelve patients(5 females, 7 males; mean age, 70 years) underwent 19 endoscopic RFA(range, 1-5) sessions. Deployment of RFA was successful in all patients. Systemic chemotherapy was administered in four patients. We observed biliary bleeding 4-6 wk after the intervention in three cases and two of these patients died: in one patient, spontaneous hemobilia occurred, whereas bleeding started during stent extraction in the other. In the third patient, bleeding was stopped by insertion of a non-covered self-expanding metal stent. Another three patients developed cholangitis during follow-up. Seven patients died during follow-up and median survival was 6.4 mo(95%CI: 0.05-12.7) from the time of the first RFA. CONCLUSION: Endoscopic RFA is an easy to perform and technically highly successful procedure. However, hemobilia possibly associated with RFA occurred in three of our patients. Therefore, larger prospective studies are needed to further evaluate the safety and efficacy of this promising new method. | Andrea Oliver Tal Johannes Vermehren Mireen FriedrichRust Jrg Bojunga Christoph Sarrazin Stefan Zeuzem Jrg Trojan Jrg Gerhard Albert | 2014 | World Journal of Gastrointestinal Endoscopy2014，6，1： | 18 |
| 3 | 4种栓塞材料行子宫动脉栓塞术后增强MRI的比较显示文摘目的 对子宫动脉栓塞术治疗后的子宫肌瘤病人行MR增强检查,验证并非所有栓塞材料具有等同效果的假设。方法 本研究获机构审查委员会批准,免除知情同意,符合HIPAA法案。84例行6个月MRI随访的女性病人被纳入本回顾性研究,分别采用Contour PVA颗粒(25例)、Contour SE颗粒(23例)、Embosphere微球(19例)、Bead Block微球(17例)治疗。 | S.D.Abramowitz G.M.Israel S.M.McCarthy J.S.Pollak R.I.White M.G. Tal 朱玉兆 | 2009 | 国际医学放射学杂志2009，32，2： | 11 |
| 4 | Obesity, fatty liver and liver cancer显示文摘BACKGROUND: It has been suggested that obesity and fat- ty liver may be associated with the morbidity and mortality of liver cancer, and the early diagnosis and effective treat- ment of fatty liver coupled with liver cancer are supposed to improve the prognosis of obese patients. This review was attempted to understand the relationship between obesity, fatty liver and liver cancer. DATA RESOURCES: An English-language literature search using PUBMED (1990-2004) on obesity, fatty liver and liv- er cancer and other related articles in Chinese. RESULTS: Obesity is associated with the risk of death from all cancers and from cancers at individual sites including liv- er cancer, and it is an independent risk factor for hepatocel- lular carcinoma (HCC) in patients with alcoholic cirrhosis and cryptogenic cirrhosis. Because nonalcoholic steatohe- patitis has been implicated as a major cause of cryptogenic cirrhosis, the development of HCC may be part of progres- sive nature of this condition. CONCLUSIONS: Obesity is associated with the incidence and mortality of HCC. More frequent surveillance for HCC may be warranted in obese patients with fatty liver and at- tempts should be made to interrupt the progression from simple hepatic steatosis to steatohepatitis, cirrhosis and ulti- mately HCC. | Yan Qian and Jian-Gao Fan Shanghai, China Center for Fatty Liver Disease, First People’ s Hospi- tal , Shanghai Jiaotong University, Shanghai 200080, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，2： | 11 |
| 5 | Multiphase hepatic scans with multirow- detector helical CT in detection of hypervascular hepatocellular carcinoma显示文摘BACKGROUND: Multirow-detector helical CT (MDCT) allows faster Z-axis coverage and improves longitudinal re- solution to scan the entire liver. This study was to evaluate the value of multiphase hepatic CT scans using MDCT in diagnosing hypervascular hepatocellular carcinoma (HCC). METHODS: Multiphase hepatic CT scans in 40 patients were carried out with a Marconi Mx8000 MDCT scanner. The scans of early arterial phase (EAP), late arterial phase (LAP) and portal venous phase (PVP) were started at 21, 34 and 85 seconds after injection of contrast medium, re- spectively. The number of detected lesions was calculated in each phase. The density of the liver and tumor was great- er than 1 cm for HCC, and the density of the liver and tumor in each phase was statistically calculated. RESULTS: A total of 61 lesions were found in the 40 pa- tients , and lesions greater than 1 cm were seen in 47 cases. The density differences between the liver and tumor were statistically significant (P<0.05) at the LAP and EAP and between the LAP, EAP and PVP. In the 61 lesions, the de- tectability in the EAP, LAP and the double arterial phases (DAP) was 32%, 87%, and 94%, respectively. Significant difference was found between the LAP plus PVP and the EAP plus PVP; but no significant difference was observed between the DAP plus PVP and the LAP plus PVP. CONCLUSIONS: The utility of MDCT scan in the liver has optimized the protocol of arterial phase scan. MDCT is possible to scan the entire liver in a real arterial phase and it is very valuable in the detection of small HCC. | Hong Zhao, Jin-Lin Yao, Ming-Jun Han, Kang-Rong Zhou and Fu-Hua Yan Zhuhai, ChinaDepartment of Radiology , and Department of Surgery Fifth Hospital, Zhongshan Universi- ty, Zhuhai 519000, China Department of Radiology, Zhongshan Hospi- tal, Fudan University, Shanghai 200032, China | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，2： | 10 |
| 6 | Relations of Budd-Chiari syndrome to prothrombin gene mutation显示文摘BACKGROUND: Budd-Chiari syndrome (BCS) is a type of disease characterized by portal hypertension and/or hy- pertension of the inferior vena cava (IVC) due to the ob- struction of the hepatic veins (HV) and/or intrahepatic IVC outlet. Being etiologically complicated and obscure, BCS can be acquired or idiopathic and several gene muta- tions may be contributable. This study was to explore whether prothrombin gene mutation (F G20210A) takes part in the pathogenesis of BCS and to investigate their cor- relativity. METHODS: In 38 proven BCS patients and 70 controls, polymerase chain reaction-restriction fragment length poly- morphism (PCR-RFLP) was used to find F G20210A mutation. To detect whether there are any mutations, four steps were taken: purification of genome DNA from whole blood, amplification of special fragment by polymerase chain reaction, digestion of the fragment via restriction en- donuclease, and analysis of results by polyacrylamide gel electrophoresis. RESULTS: F G20210A mutation was not detected in all patients and controls. CONCLUSIONS: No F G20210A mutation exists in Chi- nese patients with BCS, nor correlativity between the oc- currence of BCS and F G20210A mutation. The etiology of BCS in the Chinese needs further investigation. | Guo-Ling Lin, Pei-Qin Xu, Hua Qi, Jian-Hua Lian, Hong Zheng and Xiao-Wei Dang Zhengzhou, ChinaDepartment of General Surgery, First Affiliated Hospi- tal of Zhengzhou University the Faculty of Cytobiology and Medical Genetics, Medical School, Zhengzhou Univer- sity , Zhengzhou 450052, China | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，2： | 9 |
| 7 | Triplex operation for portal hypertension with esophageal variceal bleeding:report of 140 cases显示文摘BACKGROUND:Portal hypertension is a common dis ease. The surgical therapy of this disease focuses on the re sultant upper digestive tract bleeding, which can imperi patients’ life directly. This study was to evaluate the effect of triplex operation ( mesocaval C shunt with artificia graft, ligation of the coronary vein and splenic artery) on portal hypertension and its associated upper digestive tract bleeding. METHODS: A retrospective study was made on clinical da- ta of 140 patients undergoing triplex operation, who had suffered from portal hypertension and upper digestive tract bleeding. RESULTS: Postoperative portal pressure was 25-43 cmH2 O ( preoperative portal pressure 27-45 cmH2 O ) with the average reduction of 10 cmH2O. One patient (0.7%) died of cerebrovascular disease. Five patients (3.5%) suffered from mild hepatic encephalopathy, which was ameliorated through conservative treatment. Lymphatic fistula occurred in 3 patients (2.1% ) who recovered without treatment 5, 10 days and 3 months after operation respectively. One hundred patients were followed up for 1 month to 6 years without recurrent hemorrhage or hepatic encephalopathy. Hypersplenism and ascites disappeared in 70 patients (70% ) and 80 patients (80% ) respectively. A significant reduction of ascites was seen in 12 patients(12% ). The arti- ficial vessels remained unblocking detected by B type ultra- sonography and Doppler sonography in 95 patients (95% ). CONCLUSION: Triplex operation is suitable for patients with the following portal hypertensions; portal hyperten- sion caused by simple occlusion of the hepatic vein (a patho- logical type of Budd-Chiari syndrome); thrombosis of the portal vein or prehepatic portal hypertension because of cavernous transformation; intrahepatic portal hypertension with rebleeding after splenectomy or non-operation, and those patients with liver function in grade A or B according to the Child-Pugh classification. | Liu-Shun Feng, Ke Li, Qi-Ping Peng, Xiu-Xian Ma, Yong-Fu Zhao, Pei-Qin Xu and Xiao-Ping Chen Zhengzhou, China Department of General Surgery, First Affiliated Hospi- tal , Zhengzhou University, Zhengzhou 450052 , China Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，4： | 9 |
| 8 | Correlation of glycosyltransferases mRNA expression in extrahepatic bile duct carcinoma with clinical pathological characteristics显示文摘BACKGROUND: The incidence of extrahepatic bile duct carcinoma (EBDC) has been increasing, especially in aged people, but the glycobiology of the tumor is not elucida- ted. In this study we investigated the expressions of three glycosyltransferases in 35 patients with EBDC and 35 pa- tients with benign biliary duct disease (BBDD) as well as their clinicopathological significance. METHOD: The patients were divided into several sub- groups by tumor differentiation, TNM stage, and invasion by the standards recommended by UICC. Tumor samples were immediately frozen in liquid nitrogen after resection, followed by mRNA determination of enzymes in the tissue using a mRNA selective reverse trancriptase-polymerase chain reaction kit. The mRNA levels of different groups were semi-quantitatively compared. RESULTS: The mRNA levels of N-acetylglucosaminyltrans- ferase V (GnT-V) and a subtype of α2,3 sialyltransferases for N-glycans, ST3Gal- were elevated 7.75 and 5.39 times in EBDC as compared with BBDD, respectively, and they were correlated to several clinicopathological factors including tumor advancement, differentiation, metastasis, and invasiveness. The mRNA expression of another sialyl- transferase, ST6Gal- , was also 0.63-fold higher in EBDC than in BBDD, but not involved in the clinicopathological characteristics. CONCLUSION: The elevated expression of these three gly- cosyltransferases can be considered as an important molecu- lar event in the occurrence and progression of EBDC. | Xiao-Ling Jin, Shu-Sen Zheng, Bing-Sheng Wang and Hui-Li Chen Nanjing, China Department of General Surgery, Nanjing First Hospi- tal Affiliated to Nanjing Medical University, Nanjing 210006, China Department of Hepatobiliary Surgery, First Affiliated Hospital, Zhe- jiang University School of Medicine, Hangzhou 310003, China Department of General Surgery, Zhongshan Hospital, Fudan Universi- ty, Shanghai 200032 , China and Key Laboratory of Glycocon- jugate Research, Ministry of Health, Department of Biochemistry, Fudan University School of Medicine, Shanghai 200032, China | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，2： | 6 |
| 9 | Detection of Repair of the Zone of Calcified Cartilage with Osteoarthritis through Mesenchymal Stem Cells by Ultrashort Echo Time Magnetic Resonance Imaging显示文摘 | Quan Zhou Shao-Lin Li Ya-Jun Ma Vicki de Tal Wei Li Ying-Hua Zhao | 2018 | Chinese Medical Journal2018，，9： | 5 |
| 10 | A BFG model for calculation of tidal current and diffusion of pollutants in nearshore areas显示文摘ABFGmodelforcalculationoftidalcurrentanddiffusionofpollutantsinnearshoreareas￥ShiFengyan;andZheng;Lianyuan(StateKeyLaboratory... | Shi Fengyan and Zheng Lianyuan(State Key Laboratory of Estuarine and ￣tal Research, Institute of Estuarine and Coastal Research, East China NOrmalUniversity, Shanghai 200062, China)(Department of Marine audiences, the University of georgia. Athens. GA | 1996 | Acta Oceanologica Sinica1996，15，3： | 3 |
| 11 | Treatment of membranous Budd-Chiari syndrome: analysis of 480 cases显示文摘BACKGROUND: Budd-Chiari syndrome (BCS) presents a kind of disease resulted from the occlusion of hepatic vein and/or the intrahepatic inferior vena cava. Its different pathological types were proposed. According to our expe- rience , the membranous type takes a large part of it, and we tried to explore the best treatment of membranous BCS through the analysis of 480 cases retrospectively. METHOD: The operative results of 480 patients with mem- branous BCS were analysed retrospectively. RESULTS: Patients after Kimura’s finger rupture, inter- ventional treatment and membrane resection were followed up with rates of 84.62%, 86.55%, and 87.37%, respective- ly. The effective rates of the three methods were 61.4%, 91.7%, and 90.4%, respectively, and the recurrence rates of the disease after the 3 procedures were 38.6%, 8.3% and 9.6%, respectively. The long-term effects of interventional treatment and resection were significantly better than those of Kimura’ s finger rupture (P <0.05). CONCLUSION: Balloon dilatation is the choice for mem- branous BCS. Patients with extensive lesion, thick mem- brane or recurrence after percutaneous transhepatic angio- graphy should undergo membrane resection. | Pei-Qin Xu and Xiao-Wei Dang Zhengzhou, China Department of General Surgery, First A ffiliated Hospi- tal of Zhengzhou University, Zhengzhou 450052 , China | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，1： | 3 |
| 12 | Narrow-band imaging without high magnification to differentiate polyps during real-time colonoscopy: improvement with experience显示文摘 | Jason N. Rogart Dhanpat Jain Uzma D. Siddiqui Tal Oren Joseph Lim Priya Jamidar Harry Aslanian | 2008 | Gastrointestinal Endoscopy2008，，6： | 2 |
| 13 | Denitrification in recirculating systems: Theory and applications显示文摘 | Jaap van Rijn Yossi Tal Harold J. Schreier | 2005 | Aquacultural Engineering2005，，3： | 2 |
| 14 | Cavity optomechanics on a microfluidic resonator with water and viscous liquids显示文摘Currently,optical or mechanical resonances are commonly used in microfluidic research.However,optomechanical oscillations by light pressure were not shown with liquids.This is because replacing the surrounding air with water inherently increases the acoustical impedance and hence,the associated acoustical radiation losses.Here,we bridge between microfluidics and optomechanics by fabricating a hollow-bubble resonator with liquid inside and optically exciting vibrations with 100 MHz rates using only mW optical-input power.This constitutes the first time that any microfluidic system is optomechanically actuated.We further prove the feasibility of microfluidic optomechanics on liquids by demonstrating vibrations on organic fluids with viscous dissipation higher than blood viscosity while measuring density changes in the liquid via the vibration frequency shift.Our device will enable using cavity optomechanics for studying non-solid phases of matter,while light is easily coupled from the outer dry side of the capillary and fluid is provided using a standard syringe pump. | Kyu Hyun Kim Gaurav Bahl Wonsuk Lee Jing Liu Matthew Tomes Xudong Fan Tal Carmon | 2013 | Light(Science & Applications)2013，2，1： | 2 |
| 15 | Management of severe Budd-Chiari syndrome:report of 147 cases显示文摘BACKGROUND:Budd-Chiari syndrome (BCS) is an un- common disorder caused by the obstruction of hepatic ve- nous outflow and/or the inferior vena cava. Major thera- peutic approaches include operation and radiological inter- vention. This study was conducted to investigate the treat- ment of severe BCS. METHODS: The clinical data of 147 patients with severe BCS who had been treated at our hospital from November 1994 to December 2003 were retrospectively analyzed. RESULTS: One hundred twenty-one patients with BCS un- derwent surgery, including mesocaval C type shunt with ar- tificial graft (82 patients), splenojugular shunt (37), meso- jugular shunt (2), percutaneous transhepatic recanalization and dilatation and/or stent placement of the main hepatic vein (MHV) (12), and combined percutaneous transhepa- tic angioplasty (PTA) and stent placement of the inferior vena cava and mesocaval shunt (14). Follow-up for 6-108 months showed excellent results in 102 patients (69.4%), good results in 40 (27.2% ), and 5 deaths. CONCLUSION: Good results could be obtained in most of patients with BCS after different surgical treatments accord- ing to the pathological changes of the IVC and MHV. | Liu-Shun Feng, Qi-Ping Peng , Ke Li, Xiu-Xian Ma, Yong-Fu Zhao, Xue-Xiang Ye, Pei-Qin Xu and Xiao-Ping Chen Zhengzhou, China Department of General Surgery, First Affiliated Hospi- tal , Zhengzhou University, Zhengzhou 450052 , China and Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，4： | 2 |
| 16 | Gene delivery by lentivirus vectors显示文摘 | Adam S. Cockrell Tal Kafri | 2007 | Molecular Biotechnology2007，，3： | 2 |
| 17 | From Density to Destiny: Using Spatial Dimension of Sales Data for Early Prediction of New Product Success显示文摘 | Tal Garber Jacob Goldenberg Barak Libai Eitan Muller | 2004 | Marketing Science2004，，3： | 2 |
| 18 | 通过训练增加头胎牛产奶量并延长奶牛使用寿命显示文摘中国奶业发展非常迅速,新建牧场(通常是大牧场)的数量在逐年增加。通常牧场建好之后首先要买牛,饲养到一定时候,进行人工授精,一年之后开始挤奶。 | Amotz Tal | 2014 | 中国乳业2014，，10： | 2 |
| 19 | Mesh segmentation using feature point and core extraction显示文摘 | Sagi Katz George Leifman Ayellet Tal | 2005 | The Visual Computer (-)2005，，8： | 2 |
| 20 | Protein-Protein interactions: structurally conserved residues distinguish between binding sites and exposed protein surfaces显示文摘 | Buyong M Tal E Haim W | 2003 | Proc Natl Acad Sci USA2003，100，10： | 1 |
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