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| 1 | MAPK signal pathways in the regulation of cell proliferation in mammalian cells显示文摘MAPK families play an important role in complex cellular programs like proliferation, differentiation, development, transformation, and apoptosis. At least three MAPI(families have been characterized: extracellular signal-regulated kinase (ERK), Jun kinase (JNK/SAPK) and p38 MAPK. The above effects are fulfilled by regulation of cell cycle engine and other cell proliferation related proteins. In this paper we discussed their functions and cooperation with other signal pathways in regulation of cell proliferation. | WEI ZHANG, Hui Tu LIU The Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Sciences, Beijing Normal University, Beijing 100875, China | 2002 | Cell Research2002，12，1： | 107 |
| 2 | Discovery of laryngeal carcinoma by serum proteomic pattern analysis显示文摘Laryngeal carcinoma is the most common malignancy among head and neck tu-mors. The purpose of this study is to find biomarkers for laryngeal carcinoma in patient blood serum using the Surface Enhanced Laser Desorption/Ionization (SELDI) technique. Serum samples from 33 laryngeal carcinoma (12 cases of glottis, 18 of supraglottis and 3 of subglottis) patients and 31 age- and sex-matched healthy people were analyzed by SELDI-TOF on a Pro-teinChip reader, PBSII-C. Protein profiles were generated using WCX2 protein chips. Protein peak clustering and classification analyses were performed utilizing the Biomarker Wizard and Biomarker Pattern software packages, respectively. The results showed that sixteen peaks had significant difference between laryngeal cancer patients and healthy group, eight of which were up-regulated in the patient samples, and the others were down-regulated. Two protein peaks 8153 Da and 2035 Da were automatically chosen for the system training and development of a classification tree. The analysis yielded a correct percentage of 96.9% for patients and 96.7% for control. The results suggest that serum is a useful resource for the detection of specific bio-markers for laryngeal carcinoma. Proteinchip Array System was a useful tool for a high throughput screening of large-sized serum samples to discover potential biomarkers for carci-noma. | XIAO Xueyuan1, ZHAO Xiaodong2, LIU Jiankai3, GUO Fuzheng1, LIU Danhui1 & HE Dacheng1 1. Key Laboratory for Cell Proliferation and Regulation Biology, Ministry of Education, Beijing Normal University, Universities?Confeder-ated Institute of Proteomics, Beijing 100875, China 2. Department of Otolaryngology-Head and Neck Surgery, Third Affiliated Hospital, Jilin University, Changchun 130031, China 3. Department of Biochemistry, Jilin University, Changchun 130021, China | 2004 | Science China(Life Sciences)2004，47，3： | 22 |
| 3 | Discovery and identification of Serum Amyloid A protein elevated in lung cancer serum显示文摘Two hundred and eighteen serum samples from 175 lung cancer patients and 43 healthy individuals were analyzed by using Surface Enhaced Laser Desorption/Ionization Time of Flight Mass Spectrome- try (SELDI-TOF-MS). The data analyzed by both Biomarker Wizard? and Biomarker Patterns? software showed that a protein peak with the molecular weight of 11.6 kDa significantly increased in lung cancer. Meanwhile,the level of this biomarker was progressively increased with the clinical stages of lung cancer. The candidate biomarker was then obtained from tricine one-dimensional sodium dodecyl sul- fate-polyacrylamide gel electrophoresis by matching the molecular weight with peaks on WCX2 chips and was identified as Serum Amyloid A protein (SAA) by MALDI/MS-MS and database searching. It was further validated in the same serum samples by immunoprecipitation with commercial SAA antibody. To confirm the SAA differential expression in lung cancer patients, the same set of serum samples was measured by ELISA assay. The result showed that at the cutoff point 0.446(OD value)on the Receiver Operating Characteristic (ROC) curve, SAA could better discriminate lung cancer from healthy indi- viduals with sensitivity of 84.1% and specificity of 80%. These findings demonstrated that SAA could be characterized as a biomarker related to pathological stages of lung cancer. | DAI SongWei1,2, WANG XiaoMin1,2, LIU LiYun1,2, LIU JiFu3, WU ShanShan3, HUANG LingYun1,2, XIAO XueYuan1,2 & HE DaCheng1,2 1 Key Laboratory of Cell Proliferation and Regulation of Ministry of Education, Beijing Normal University, Beijing 100875, China 2 Universities’ Confederated Institute of Proteomics, Beijing 100875, China 3 Department of Thoracic Surgery, General Hospital of Beijing Unit, PLA, Beijing 100500, China | 2007 | Science China(Life Sciences)2007，50，3： | 10 |
| 4 | Senescence-like changes induced by expression of p21^(Waf1/Cip1) in NIH3T3 cell line显示文摘P21Waf1/Cip1 is a potent cyclin-dependent kinase inhibitor. As a downstream mediator of p53, p21Waf1/cip1involves in cell cycle arrest, differentiation and apoptosis. Previous studies in human cells provided evidencefor a link between p21Waf1/cip1 and cellular senescence. While in murine cells, the role of p21Waf1/Cip1is indefinite. We explored this issue using NIH3T3 cells with inducible p21Waf1/cip1 expression. Induc-tion of p21Waf1/Cip1 triggered G1 growth arrest, and NIH3T3-p21 cells exhibited morphologic features,such as enlarged and flattened cellular shape, specific to the senescence phenotype. We also showed thatp21Waf1/Cip1-transduced NIH3T3 cells expressedβ-galactosidase activity at pH 6.0, which is known to bea marker of senescence. Our results suggest that p21Waf1/cipx can also induce senescence-like changes inmurine cells. | XI CHEN1, WEI ZHANG2, YUN FEI GAO1, XIAO QIN SU1, ZHONG HE ZHAI1,*1 College of Life Sciences, Peking University, Beijing 100871, China2 The Key Laboratory of Cell Proliferation and Regulation Biology of the Ministry of Education, College of Life Sciences, | 2002 | Cell Research2002，12，4： | 9 |
| 5 | Generation of pancreatic islet cells from human embryonic stem cells显示文摘Efficiently obtaining functional pancreatic islet cells derived from human embryonic stem(hES) cells not only provides great potential to solve the shortage of islets sources for type I diabetes cell therapy,but also benefits the study of the development of the human pancreas and diabetes pathology.In 2001,hES cells were reported to have the capacity to generate insulin-producing cells by spontaneous differentiation in vitro.Since then,many strategies(such as overexpression of key transcription factors,delivery of key proteins for pancreatic development,co-transplantation of differentiated hES cells along with fetal pancreas,stepwise differentiation by mimicking in vivo pancreatic development) have been employed in order to induce the differentiation of pancreatic islet cells from hES cells.Moreover,patient-specific induced pluripotent stem(iPS) cells can be generated by reprogramming somatic cells.iPS cells have characteristics similar to those of ES cells and offer a new cell source for type I diabetes cell therapy that reduces the risk of immunologic rejection.In this review,we summarize the recent progress made in the differentiation of hES and iPS cells into functional pancreatic islet cells and discuss the challenges for their future study. | ZHANG DongHui1,2,JIANG Wei1,SHI Yan2 & DENG HongKui1,2 1 Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education,College of Life Sciences,Peking University,Beijing 100871,China 2 Laboratory of Chemical Genomics,Shenzhen Graduate School of Peking University,Shenzhen 518055,China | 2009 | Science China(Life Sciences)2009，52，7： | 6 |
| 6 | Effects of nitrogen ion implantation on Ca^(2+) concentration and membrane potential of pollen cell显示文摘The effects of low energy nitrogen ion implantation on Ca2+ concentration and membrane potential of illy (Mum davidii Duch) pollen cell have been studied. The results showed that the Ca2+ concentration was increased when pollen grain was implanted by nitrogen ion with energy 100 keV and dose 1013 ions/cm2. However, the increase of Ca2+ concentration was partly inhibited by the addition of Ca2+ channel inhibitor depending on dose. And nitrogen ion implantation caused depolarization of pollen cell membrane potential. In other words, membrane potential was increased, but the effect decreased by adding Ca2+ channel inhibitor. However, it was still significantly higher than the membrane potential of control cells. It was indicated that the depolarization of cell membrane potential opened the calcium channel on the membrane that caused the increasing of intracel-lular calcium concentration. This might be an earlier step of the effect of low energy nitrogen ion implantation on pollen germination. | HUANG Zhonglian, JING Yanping, ZHU Guoli, LU Ting, ZHOU Hongyu & REN Haiyun1. Key Laboratory of Cell Proliferation and Regulation Biology of the Ministry of Education, Beijing Normal University, Beijing 100875, China 2. Key Laboratory of Plant Physiology and Biochemistry of the Ministry of Agriculture, China Agriculture University, Beijing 100094, China 3. Institution of Low Energy Nuclear Physics, Beijing Normal University, Beijing 100875, China | 2001 | Chinese Science Bulletin2001，46，20： | 5 |
| 7 | The Villin/Gelsolin/Fragmin Superfamily Proteins in Plants显示文摘viliin/gelsolin/fragmin 总科两个都是广泛地在场的在的调整肌动朊的蛋白质 -dependentfamily 是保存 Ca^(2+) 哺乳动物并且 non-mammalianorganisms。他们被一样的英国管文件结束传统地描绘了三或六 tandemgelsolin 子域。在脊椎动物和更低的真核细胞的房间的学习表明了蛋白质的 thattheviliin/gelsolin/fragmin 总科包括切断有万用的功能,盖住,起核心作用或捆绑肌动朊细丝。在植物,令人鼓舞的进步在最近的年里在研究的这个领域里被取得了。这评论将总结识别植物相当或相同的事物 ofviliin/gelsolin/fragmin 总科,因此在植物在 theirbiochemicalactivities 和功能上为思考提供一个基础。 | Hui Su Ting Wang Huaijian Dong Haiyun Ren (Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education and College of Life Sciences Beijing Normal University Beijing 100875 China | 2007 | Journal of Integrative Plant Biology2007，49，8： | 3 |
| 8 | Short exposure to paclitaxel induces multipolar spindle formation and aneuploidy through promotion of acentrosomal pole assembly显示文摘Paclitaxel is a widely used microtubule drug and cancer medicine. Here we report that by short exposure to paclitaxel at a low dose, multipolar spindles were induced in mitotic cells without centrosome amplification. Both TPX2 depletion and Aurora-A overexpression antagonized the multipolarity. Live cell imaging showed that some paclitaxel-treated cells accomplished multipolar cell division and a portion of the daughter cells went on to the next round of mitosis. The surviving cells grew into clones with varied genome content. The results indicated that an aneuploidy population could be induced by short exposure to paclitaxel at a low dose, implicating potential side effects of paclitaxel. | BIAN MingLei1, FU JingYan1, YAN Yan1, CHEN Qiang1, YANG Chao2, SHI QingHua2,JIANG Qing1 & ZHANG ChuanMao1 1MOE Key Laboratory of Cell Proliferation and Differentiation and the State Key Laboratory of Bio-membrane and Membrane Bio-engineering, College of Life Sciences, Peking University, Beijing 100871, China 2Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei 230026, China | 2010 | Science China(Life Sciences)2010，53，11： | 3 |
| 9 | Effect of PKCα on the gene expression related with proliferation and activity of transcription factor AP-1显示文摘PROTEIN kinase C (PKC) is a serine/thronine kinase, which plays a major role in cell sig-nalling, growth control and tumorigenesis. PKC is encoded by a multigene family, which havebeen identified as consisting of at least 12 members in mammalian tissues. PKC isoforms differin their biochemical properties, tissue-specific expression and intracellular localization. Thedifferences imply that the individual PKC isoforms have a distinct physiological function. Inorder to study the role of specific PKC subtype in cell proliferation and transformation, weconstructed the human embryonic lung cell (2BS) model stably overexpressing PKC_α andfound that the overexpression of PKC_α promoted the growth rate and caused the transform- | WANG Xiangyang and LIU HuituKey Laboratory of Cell Proliferation and Regulation Biology, Department of Biology, Beijing Normal University, Beijing 100875, China | 1997 | Chinese Science Bulletin1997，42，23： | 3 |
| 10 | Identification and functional analysis of a CDE/CHR element in the POLD1 promoter显示文摘DNA polymerase delta is encoded by the POLD1 gene,the transcription of which is strictly cell cycle-dependent.However,the means by which POLD1 transcription is regulated by the cell cycle mechanism is currently unknown.We discovered a novel element in the POLD1 promoter known as a CDE(cell cycle-dependent element)/CHR(cell cycle gene homology region) element.A series of luciferase reporter constructs containing various POLD1 promoter mutations were used to investigate the role of the CDE/CHR element in POLD1 transcription.When the CDE/CHR element was mutated,the promoter activity was up-regulated,and the cell-cycle related factors E2F1 and p21 stopped regulating the promoter.Furthermore,cell cycle-dependent changes in the promoter activity required the integrative CDE/CHR element.Electrophoretic mobility shift assay(EMSA) revealed the presence of at least three types of DNA/protein complexes binding to the CDE/CHR element.Our findings provide strong evidence that the CDE/CHR-like sequence is an active functional element in the POLD1 promoter,which is important for the cell cycle regulation of the POLD1 gene. | SONG NanMeng1,ZHU XiaoYu1,2,SHI Lei1,AN Jing1,WU YanWei1 & SANG JianLi1 1 Key Laboratory of Cell Proliferation and Regulation of Ministry of Education,Beijing Normal University,Beijing 100875,China 2 State Key Laboratory for Infectious Disease Prevention and Control,Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China | 2009 | Science China(Life Sciences)2009，52，6： | 2 |
| 11 | in diabetic caucasians: Identification of a P12A PPARγ2 Missense Mutation 显示文摘 | Yen CJ Beamer BA Negri C Molecular scanning of the human peroxisome proliferator activated receptorγ gene 8 | 1997 | Biochem Biophys Res Commun1997，241，： | 1 |
| 12 | Effect of dynamic 3-D culture osteogenic differentiation of human mesenchymal stem cells 显示文摘 | Stiehler M Bunger C Baatrup A on proliferation distribution and | 2009 | J Biomed Mater Res A2009，89，1： | 1 |
| 13 | Endothelin-1 Stimulates Trophoblasts via the A- and B-Type Receptor and Invasion via the B-Type Receptor显示文摘 | Cervar Z M Dieber R M Barth Proliferation of First-Trimester S | 2011 | J Clin Endocrinol Metab2011，96，11： | 1 |
| 14 | Acetylcholine-induced of fibroblasts and myofibroblasts in vitro is tiotropium bromide 显示文摘 | Pieper M P proliferation inhibited by Chaudhary N I Park J E | 2007 | Life Sci2007，80，2425： | 1 |
| 15 | TIMP-3 promotes apoptosis显示文摘 | Baker AH Zaltsman AB George SJ Divergent effects of tissue inhibitor of metalloproteinase-1 -2 or 3 overexpression on rat vascular smooth muscle cell invasion proliferation and death in vitro | 1998 | J Clin Invest1998，101，6： | 1 |
| 16 | Toxicol显示文摘 | McCabe MJ Singh KP Reiners JJ Low level lead exposure in vitro stimulates the proliferation and expansion of alloantigen-reactive CD4high T cells | 2001 | Appl Pharmacol2001，177，： | 1 |
| 17 | Convergence at the cyclin E/p27(Kip1) regulatory checkpoint显示文摘 | Antonov AS Munn DH Kolodgie FD Aortic endo- thelial cells regulate proliferation of human monocytes in vitro via a mechanism synergistic with macrophage colo- ny-stimulating factor | 1997 | J Clin Invest1997，99，12： | 1 |
| 18 | Markers Apoptosis, and Angiogenesis in Thyroid Adeno- and Genetic Investigation of Functioning and Nonfunctioning Nodules显示文摘 | Paolo Viacava of Cell Proliferation mas: A Comparative Guido Bocci Massimo Tonaechera | 2007 | THYROID2007，17，3： | 1 |
| 19 | Noninvasive imaging of cell extracellular kinase inhibition byPD0325901 显示文摘 | J Leyton G Smith M Lees proliferation following mitogenic | 2008 | Mol Cancer Ther2008，7，9： | 1 |
| 20 | Apoptosis, Bcl-2 and Bax expres- sion in obstructed opossum early metanephroi 显示文摘 | Liapis H Yu H proliferation SC | 2000 | J Urol2000，164，2： | 1 |
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