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| 1 | Synthesis of surface imprinted polymers based on wrinkled flower-like magnetic graphene microspheres with favorable recognition ability for BSA显示文摘Nowadays,the employing of molecular imprinting technique in the analysis and separation of proteins from complex biological samples has been widely favored by researchers.To enrich the types of surface protein imprinted materials and expand the application fields of graphene materials,novel surface molecular imprinted polymers(MIPs)based on magnetic graphene microspheres Fe_(3)O_(4)@r GO@MIPs are first synthesized in this paper.Fe_(3)O_(4)@r GO@MIPs are prepared by oxidative self-polymerization of dopamine on the surface of magnetic graphene(Fe_(3)O_(4)@r GO)composite microspheres.Bovine serum albumin(BSA)is selected as protein template.Fe_(3)O_(4)@r GO microspheres with wrinkled flower-like structure are obtained by compounding Fe_(3)O_(4)and graphene oxide in an appropriate ratio via the method of high-temperature reduction self-assembly.The microspheres exhibit promising dispersibility,high external surface area,rich pore structure,and sufficient magnetic properties.These advantages not only prevent the agglomeration of imprinted microspheres in the aqueous phase,which is conducive to contact and static adsorption,but also increase the amount of protein imprinting.Additionally,sufficient magnetic properties ensure fast and effective separation of the adsorbents.While the adsorption capacity is increased,the separation procedure becomes simple.The binding capacity of Fe_(3)O_(4)@r GO@MIPs for BSA can reach 317.58 mg/g within 60 min,and the imprinting factor(IF)is 4.24.More importantly,Fe_(3)O_(4)@r GO@MIPs can specifically recognize the target BSA from the mixed proteins and the actual sample.There is no significant decrease in the adsorption amount,IF,and magnetic properties after eight runs.It is promising to be used in the separation of proteins from the actual biological samples. | Zuoting Yang Ke Yang Yuhong Cui Tariq Shah Mudasir Ahmad Qiuyu Zhang Baoliang Zhang | 2021 | Journal of Materials Science & Technology2021，，15： | 2 |
| 2 | Changesofhigherorderaberrationwithvariouspupilsizesinthemyopiceye显示文摘 | WangY ZhaoK JinY NiuY ZuoT | 2003 | JRefractSurg2003，19，2： | 1 |
| 3 | Ocularhigherorderaberrationsfeaturesanalysisaftercornealrefractivesurgery显示文摘 | WangY ZhaoKX HeJC JinY ZuoT | 2007 | ChinMedJ(Engl)2007，120，4： | 1 |
| 4 | SWOTanalysisofGuangxiseedindustrydevelopmentbasedonbackgroundofChina-ASEANFreeTradeArea(中国-东盟自由贸易区背景下广西种业发展的SWOT分析)显示文摘 | HELJ(何礼健) ZUOT(左停) | 2011 | SouthwestChinaJournalofAgriculturalSciences2011，24，2： | 1 |
| 5 | FOXP3 is an X-linked breast cancer suppressor gene and an important represser of the HER-2/ErbB2 oncogene显示文摘 | Wang L Morrison C | 2007 | Cell2007，129，7： | 1 |
| 6 | A Novel Approach of Network Coding in Wireless Networks Overhearing显示文摘Network coding is proved to improve the throughput, decrease network latency and balance the network overhead. Combined with wireless overhearing, some proposed representative schemes on network coding, such as linear network coding and Completely opportunity encoding(COPE). The former scheme is based on complicated linear operation, while the latter is implemented by the way of pairwise XOR-ing which is simple and low efficiency. In order to resolve these problems, we put forward a group-based XOR-ing network coding scheme, which has the characteristics of high efficiency and low complexity.Experimental results and theoretical analysis demonstrate that our scheme has higher throughput gains and lower delay than traditional schemes. In addition, our proposed scheme has the best characteristic of fairness among the state-of-the-art approaches. | NING Zuoting ZHANG Dafang LIU Xuchong | 2018 | Chinese Journal of Electronics2018，27，6： | 0 |
| 7 | Sulforaphane prevents LPS‑induced inflammation by regulating the Nrf2‑mediated autophagy pathway in goat mammary epithelial cells and a mouse model of mastitis显示文摘Background Mastitis not only deteriorates the composition or quality of milk,but also damages the health and pro-ductivity of dairy goats.Sulforaphane(SFN)is a phytochemical isothiocyanate compound with various pharmacologi-cal effects such as anti-oxidant and anti-inflammatory.However,the effect of SFN on mastitis has yet to be elucidated.This study aimed to explore the anti-oxidant and anti-inflammatory effects and potential molecular mechanisms of SFN in lipopolysaccharide(LPS)-induced primary goat mammary epithelial cells(GMECs)and a mouse model of mastitis.Results In vitro,SFN downregulated the mRNA expression of inflammatory factors(tumor necrosis factor-α(TNF-α),interleukin(IL)-1βand IL-6),inhibited the protein expression of inflammatory mediators(cyclooxygenase-2(COX2),and inducible nitric oxide synthase(iNOS))while suppressing nuclear factor kappa-B(NF-κB)activation in LPS-induced GMECs.Additionally,SFN exhibited an antioxidant effect by increasing Nrf2 expression and nuclear translocation,up-regulating antioxidant enzymes expression,and decreasing LPS-induced reactive oxygen species(ROS)produc-tion in GMECs.Furthermore,SFN pretreatment promoted the autophagy pathway,which was dependent on the increased Nrf2 level,and contributed significantly to the improved LPS-induced oxidative stress and inflammatory response.In vivo,SFN effectively alleviated histopathological lesions,suppressed the expression of inflammatory factors,enhanced immunohistochemistry staining of Nrf2,and amplified of LC3 puncta LPS-induced mastitis in mice.Mechanically,the in vitro and in vivo study showed that the anti-inflammatory and anti-oxidative stress effects of SFN were mediated by the Nrf2-mediated autophagy pathway in GMECs and a mouse model of mastitis.Conclusions These results indicate that the natural compound SFN has a preventive effect on LPS-induced inflam-mation through by regulating the Nrf2-mediated autophagy pathway in primary goat mammary epithelial cells and a mouse model of mastitis,which may improve prevention strategies for mastitis in dairy goats. | Dan Shao Wenxiang Shen Yuyang Miao Zhen Gao Menghao Pan Qiang Wei Zuoting Yan Xiaoe Zhao Baohua Ma | 2023 | Journal of Animal Science and Biotechnology2023，14，5： | 0 |
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