|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Efficient Gene Targeting in Zebrafish Mediated by a Zebrafish-Codon-Optimized Cas9 and Evaluation of Off-Targeting Effect显示文摘Targeted genome modifications with the Cas9/gRNA system derived from the clustered regularly interspaced short palindromic repeat/CRISPR-associated(CRISPR/Cas)system have been successfully used in cultured human cells as well as in most model organisms,including zebrafish(Danio rerio),mouse,and fruit fly(Chang et al.,2013;Cong et al.,2013; | Da Liu Zhanxiang Wang An Xiao Yutian Zhang Wenyuan Li Yao Zu Shaohua Yao Shuo Lin Bo Zhang | 2014 | Journal of Genetics and Genomics2014，41，1： | 18 |
| 2 | Rapid quantification of hepatitis B virus DNA by real-time PCR using efficient TaqMan probe and extraction of virus DNA显示文摘AIM: To rapidly quantify hepatitis B virus (HBV) DNA by real-time PCR using efficient TaqMan probe and extraction methods of virus DNA. METHODS: Three standards were prepared by cloning PCR products which targeted S, C and X region of HBV genome into pGEM-T vector respectively. A pair of primers and matched TaqMan probe were selected by comparing the copy number and the Ct values of HBV serum samples derived from the three different standard curves using certain serum DNA. Then the efficiency of six HBV DNA extraction methods including guanidinium isothiocyanate, proteinase K, NaI, NaOH lysis, alkaline lysis and simple boiling was analyzed in sample A, B and C by real-time PCR. Meanwhile, 8 clinical HBV serum samples were quantified. RESULTS: The copy number of the same HBV serum sample originated from the standard curve of S, C and X regions was 5.7 × 104/mL, 6.3 × 102/mL and 1.6 × 103/ mL respectively. The relative Ct value was 26.6, 31.8 and 29.5 respectively. Therefore, primers and matched probe from S region were chosen for further optimization of six extraction methods. The copy number of HBV serum samples A, B and C was 3.49 × 109/mL, 2.08 × 106/mL and 4.40 × 107/mL respectively, the relative Ct valuewas 19.9, 30 and 26.2 in the method of NaOH lysis, which was the efficientest among six methods. Simple boiling showed a slightly lower efficiency than NaOH lysis. Guanidinium isothiocyanate, proteinase K and NaI displayed that the copy number of HBV serum sample A, B and C was around 105/mL, meanwhile the Ct value was about 30. Alkaline failed to quantify the copy number of three HBV serum samples. Standard deviation (SD) and coefficient variation (CV) were very low in all 8 clinical HBV serum samples, showing that quantification of HBV DNA in triplicate was reliable and accurate. CONCLUSION: Real-time PCR based on optimized primers and TaqMan probe from S region in combination with NaOH lysis is a simple, rapid and accurate method for quantification of HBV serum DNA. | Yan-Qin Lu Jin-Xiang Han Peng Qi Wei Xu Yan-Hui Zu Bo Zhu | 2006 | World Journal of Gastroenterology2006，12，45： | 8 |
| 3 | Lipid nanoparticle-encapsulated mRNA antibody provides longterm protection against SARS-CoV-2 in mice and hamsters显示文摘Monoclonal antibodies represent important weapons in our arsenal to against the CO VID-19 pandemic.However,this potential is severely limited by the time-consuming process of developing effective antibodies and the relative high cost of manufacturing.Herein,we present a rapid and cost-effective lipid nanoparticle(LNP)encapsulated-mRNA platform for in vivo delivery of SARS-CoV-2 neutralization antibodies.Two mRNAs encoding the light and heavy chains of a potent SARS-CoV-2 neutralizing antibody HB27,which is currently being evaluated in clinical trials,were encapsulated into clinical grade LNP formulations(named as mRNA-HB27-LNP).I n vivo characterizatio n dem on strated that intravenous administratio n of mRNA-HB27-LNP in mice resulted in a longer circulating half-life compared with the original HB27 antibody in protein format.More importantly,a single prophylactic administration of mRNA-HB27-LNP provided protection against SARS-CoV-2 challenge in mice at 1,7 and even 63 days post administration.In a close contact transmission model,prophylactic administration of mRNA-HB27-LNP prevented SARS-CoV-2 in fecti on betwee n hamsters in a dose-depende nt mariner.Overall,our results dem on strate a superior Ion g-term protect!on agai nst SARS-CoV-2 conferred by a single administration of this unique mRNA antibody,highlight!ng the potential of this universal platform for antibody-based disease prevention and therapy against COVID-19 as well as a variety of other infectious diseases. | Yong-Qiang Deng Na-Na Zhang Yi-Fei Zhang Xia Zhong Sue Xu Hong-Ying Qiu Tie-Cheng Wang Hui Zhao Chao Zhou Shu-Long Zu Qi Chen Tian-Shu Cao Qing Ye Hang Chi Xiang-Hui Duan Dan-Dan Lin Xiao-Jing Zhang Liang-Zhi Xie Yu-Wei Gao Bo Ying Cheng-Feng Qin | 2022 | Cell Research2022，32，4： | 5 |
| 4 | Natural exosome-like nanovesicles from edible tea flowers suppress metastatic breast cancer via ROS generation and microbiota modulation显示文摘Although several artificial nanotherapeutics have been approved for practical treatment of metastatic breast cancer,their inefficient therapeutic outcomes,serious adverse effects,and high cost of mass production remain crucial challenges.Herein,we developed an alternative strategy to specifically trigger apoptosis of breast tumors and inhibit their lung metastasis by using natural nanovehicles from tea flowers(TFENs).These nanovehicles had desirable particle sizes(131 nm),exosome-like morphology,and negative zeta potentials.Furthermore,TFENs were found to contain large amounts of polyphenols,flavonoids,functional proteins,and lipids.Cell experiments revealed that TFENs showed strong cytotoxicities against cancer cells due to the stimulation of reactive oxygen species(ROS)amplification.The increased intracellular ROS amounts could not only trigger mitochondrial damage,but also arrest cell cycle,resulting in the in vitro anti-proliferation,anti-migration,and anti-invasion activities against breast cancer cells.Further mice investigations demonstrated that TFENs after intravenous(i.v.)injection or oral administration could accumulate in breast tumors and lung metastatic sites,inhibit the growth and metastasis of breast cancer,and modulate gut microbiota.This study brings new insights to the green production of natural exosome-like nanoplatform for the inhibition of breast cancer and its lung metastasis via i.v.and oral routes. | Qiubing Chen Qian Li Yuqi Liang Menghang Zu Nanxi Chen Brandon S.B.Canup Liyong Luo Chenhui Wang Liang Zeng Bo Xiao | 2022 | Acta Pharmaceutica Sinica B2022，12，2： | 4 |
| 5 | Room-temperature production of silver-nanofiber film for large-area, transparent and flexible surface electromagnetic interference shielding显示文摘A kind of pollution known as electromagnetic interference(EMI),which results from ubiquitous usage of various electronic communication and military radar equipment,has been receiving increasing attention recently.However,large-area EMI shielding on transparent and/or curved surfaces,including building windows,curved glass wall,and special requirements spaces(SRSs),remains hard to achieve.In this paper,a silver nanofiber(AgNF)based flexible and transparent EMI shielding film was successfully assembled via a room-temperature roll-to-roll production method.For transparent application scenario,AgNF with 89%transmittance in visible range and 1μm thickness shows~20 dB shielding efficiency(EMI SE).On the other hand,total shielding(>50 dB)is obtained when the thickness of AgNF increases to 10μm,while its transmittance in visible range remains higher than 75%.Considering the facile and scale-free production technology,this material can be readily applied in large-scale,transparent,and/or SRSs EMI shielding. | Sen Lin Haiyang Wang Fan Wu Qingmin Wang Xiaopeng Bai Di Zu Jianan Song Dong Wang Zhenglian Liu Ziwei Li Nian Tao Kai Huang Ming Lei Bo Li Hui Wu | 2019 | npj Flexible Electronics2019，3，1： | 4 |
| 6 | Chelating agents role on phase formation and surface morphology of single orthorhombic YMn_2O_5 nanorods via modified polyacrylamide gel route显示文摘YMn2O5nanorods were synthesized through a modified polyacrylamide gel route.The synthesis strategy in this work is based on a sol-gel process using a polyacrylamide gel method in which oxalic acid,citric acid or tartaric acid is employed as the chelating agent.In the gel routes,oxalic acid was used as a carboxyl chelating agent,while citric acid or tartaric acid was a carboxyl and hydroxyl chelating agent.The as-prepared samples were characterized by means of techniques such as X-ray powder diffraction(XRD)measurement,thermogravimetric analysis(TG),differential scanning calorimetry analysis(DSC),Fourier transform infrared spectroscopy(FTIR),X-ray photoelectron spectroscopy(XPS),thermal expansion measurement and field-emission scanning electron microscopy(SEM)investigations.It was found that oxalic acid is the best chelating agent with Y(NO3)3·6H2O and Mn(CH3COO)2·4H2O as precursors to prepare a single orthorhombic YMn2O5nanorods at 1000°C.Scanning electron microscope observation shows that the morphology of YMn2O5powders is significantly dependent on the chelating agent.The peaks(single orthorhombic YMn2O5nanorods)at 642,600,573,546,521,493,486,468,448 and 400cm?1were observed from FTIR spectra.The phase,surface morphology and chelation mechanisms of YMn2O5samples have been discussed on the basis of the experimental results. | WANG ShiFa ZHANG ChuanFei SUN GuangAi CHEN Bo XIANG Xia DING QingPing ZU XiaoTao | 2014 | Science China Chemistry2014，57，3： | 4 |
| 7 | Two New Compounds from Melanosciadum pimpinelloideum H. Boiss显示文摘Two new compounds, melanochromone and 2-ethoxyl-2-(4-hydroxyphenyl)ethanol, were isolated from the whole plants of Melanosoiadum pimpinelloideum H. Boiss. The known compounds isolated were 1-(4-hydroxyphenyl)-1,2-ethanediol, tymine, cimifugin, umtatin, bergenin, daucosterol and stigmasterol. Their structures were determined on the basis of spectral data. | Yi ZHOU2, Guo Lin ZHANG1*, Bo Gang LI1, Yao Zu CHEN2, 3 1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041 2Chemistry Department, Zhejiang University, Hangzhou 310027 3National Key Laboratory of Applied Organic Chemistry, Lanzhou | 2001 | Chinese Chemical Letters2001，12，4： | 3 |
| 8 | A New Flavonol Glycoside from Anaphalis sinica Hance显示文摘A new flavonol glycoside, 5,7,3,4 tetrahydroxy 3 methoxyflavonol 3 O β D glucopyranoside(1) was isolated along with quercetin(2), quercetin 3 O α L rhamnopyranoside(3), quercetin 3 O β D glucopyranoside(4) and kaempferol 3 O β D (6' O p coumaroyl) glucopyranoside(5) from Anaphalis sinica Hance. Their structures were determined on the basis of spectral data(MS, UV, IR, 1H and 13 C NMR). The structure of compound 1 was confirmed by X ray crystallographic analysis. | ZHOU Yi 1, ZHANG Guo lin 2,** , LI Bo gang 2 and CHEN Yao zu 3(1. Department of Chemistry, Zhejiang University, Hangzhou 310027, P.R. China 2. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, P.R. China 3. National | 2001 | Chemical Research in Chinese Universities2001，17，1： | 2 |
| 9 | Multi-responsive nanotheranostics with enhanced tumor penetration and oxygen self-producing capacities for multimodal synergistic cancer therapy显示文摘Incorporation of multiple functions into one nanoplatform can improve cancer diagnostic efficacy and enhance anti-cancer outcomes. Here, we constructed doxorubicin(DOX)-loaded silk fibroinbased nanoparticles(NPs) with surface functionalization by photosensitizer(N770). The obtained nanotheranostics(N770-DOX@NPs) had desirable particle size(157 nm) and negative surface charge(-25 m V). These NPs presented excellent oxygen-generating capacity and responded to a quadruple of stimuli(acidic solution, reactive oxygen species, glutathione, and hyperthermia). Surface functionalization of DOX@NPs with N770 could endow them with active internalization by cancerous cell lines, but not by normal cells. Furthermore, the intracellular NPs were found to be preferentially retained in mitochondria, which were also efficient for near-infrared(NIR) fluorescence imaging, photothermal imaging,and photoacoustic imaging. Meanwhile, DOX could spontaneously accumulate in the nucleus. Importantly, a mouse test group treated with N770-DOX@NPs plus NIR irradiation achieved the best tumorretardation effect among all treatment groups based on tumor-bearing mouse models and a patientderived xenograft model, demonstrating the unprecedented therapeutic effects of trimodal imagingguided mitochondrial phototherapy(photothermal therapy and photodynamic therapy) and chemotherapy.Therefore, the present study brings new insight into the exploitation of an easy-to-use, versatile, and robust nanoplatform for programmable targeting, imaging, and applying synergistic therapy to tumors. | Shuangquan Gou Nanxi Chen Xiaoai Wu Menghang Zu Shixiong Yi Binwu Ying Fangyin Dai Bowen Ke Bo Xiao | 2022 | Acta Pharmaceutica Sinica B2022，12，1： | 2 |
| 10 | Surface modification on nanoscale titanium dioxide by radiation: preparation and characterization 显示文摘 | JIANG Bo ZU Xiaotao | 2006 | J Appl Polym Sci2006，100，5： | 1 |
| 11 | Post-melting anomaly of Pb-Bi alloys observed by internal friction technique显示文摘 | ZU Fang-qiu ZHU Zhen-gang ZHANG Bo FENG Yi SHUI Jia-peng | 2001 | Journal of Physics: Condensed Matter2001，13，11： | 1 |
| 12 | Enrichmentcultivation of ammonia oxidizer from normal activa-ted sludge 显示文摘 | Zu Bo Zhang Daijun Lu Peili | 2005 | Journal of Chongqing University2005，28，2： | 1 |
| 13 | Single-drop microextraction combined with low-temperature electrothermal vaporization ICPMS for the determination of trace Be,Co,Pd,and Cd in biological samples显示文摘 | XIA Lin Bo HU Bin JIANG Zu Cheng | 2004 | Anal Chem2004，76，10： | 1 |
| 14 | Surface modification on nanoscale titanium dioxide by radiation: preparation and characterization显示文摘 | Jiang Bo Zu Xiaotao Tang Fangyuan | 2006 | Journal of Applied Polymer Science2006，100，05： | 1 |
| 15 | Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafish显示文摘 | An Xiao Zhanxiang Wang Yingying Hu Yingdan Wu Zhou Luo Zhipeng Yang Yao Zu Wenyuan Li Peng Huang Xiangjun Tong Zuoyan Zhu Shuo Lin Bo Zhang | 2013 | Nucleic Acids Research2013，，14： | 1 |
| 16 | Underlying Mechanism of Aconitum Lizhong Acting on Experimental Hypothermia with Indigestion in Rats: Role of Ghrelin显示文摘 | Xin Zhao Yong Wang Shijun Yang Wentong Zhang Chengzhe Zu Taotao Zhang Binghua Tang Baochun Zhang Guozhang Li Bo Deng Dayong Cai Bhushan Patwardhan | 2012 | Evidence-Based Complementary and Alternative Medicine2012，，： | 1 |
| 17 | Fast algorithm based on LBP texture histogram for background modeling on CUDA 显示文摘 | Zu Zhong-lin Li Bo Chen Qi-mei | 2011 | Journal of Wuhan University of Technology:Transpor- tation Science Engineering2011，，： | 1 |
| 18 | Effects of heat shock protein-antigen peptide complexes (HACs) in melanoma B16 cell line on transplanted tumor development in mice显示文摘目的 :建立黑色素瘤 B16细胞热休克蛋白 -抗原肽复合物 (HACs)的制备方法并测其抑瘤效应。方法 :应用 Sephacryl S- 2 0 0凝胶过滤制备 HAC粗提物 ,应用 SDS- PAGE纯化 HACs,并测其抑瘤效应。结果 :应用 SDS- PAGE纯化的 HAC6 0、 HAC75和 HAC97不同程度地降低肿瘤发生率 ,延迟肿瘤发生时间和减慢肿瘤生长速度。结论 :6 0 0 0 0~ 970 0 0 | GONG Shou liang,YANG Ying,LI Xiu juan,FU Shi bo,SUN Zu yue,CHEN Sha li,LI Xiu yi (MH Radiobiology Research Unit,Jilin University,Changchun 130021 China) | 2001 | 白求恩医科大学学报2001，27，5： | 0 |
| 19 | Extraction kinetics of phenol with N, N-di (1-methyl-heptyl)acetamide-kerosene using hollow fiber membrane extractor显示文摘Extraction kinetics of phenol with N,N di(1 methyl heptyl) acetamide kerosene using hollow fiber membrane extractor has been studied. The rate regularities and kinetic types of forward and backward extraction were obtained respectively by determining the forward and backward extraction rate under various experimental conditions. The mass transfer mechanism was discussed. Both the forward and backward extraction of phenol might be controlled by diffusion processes, and the diffusion resistance for both forward and backward extraction mainly exists in aqueous phase. In addition, ways to optimize the extraction process of phenol were discussed as well. | YANG Zhu xian, DU Hui fang, SUN Xian bo, XIA Ye, ZHOU Zu ming(Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China. | 2001 | Journal of Environmental Sciences2001，13，3： | 0 |
| 20 | Oligostilbentes from Vitis DavidII显示文摘A novel tetrastilbene, davidol A, was isolated from the stems of Vitis davidii together with the previously known resveratrol, (+)-ε-viniferin; ampelopsin C; ampelopsin E; vitisin A and hopeaphenol. Its structUre was elucidated mainly by 1D and 2D NMR analyses. | Wen Wu LI Bo Gang LI Yao Zu CHEN(Chengdu Institute of Biology, Academia Sinica, Chengdu 610041)(Department of Chemistry, Zhejiang University, Hangzhou 310027) | 1998 | Chinese Chemical Letters1998，9，8： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员