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| 1 | Regulatory effect and mechanism of gastrin and its antagonists on colorectal carcinoma显示文摘AIM To explore the effect and mechanism ofgastrin and its antagonists proglumide and somatostatin on coIorectal carcinoma and their clinical significance.METHODS A model of transplanted human colonic carcinoma was established from SW480cell line in gymnomouse body. The volume andweight of transplanted carcinoma was observedunder the effect of pentagatrin (PG), proglumide (PGL) and octapeptide somotostatin (SMS201-995, SMS). The cAMP content ot carcinoma cell was determined by redioimmunoassay and the DNA, protein content and cell cycle were determined by flow-cytometry. The amount of viabIe cells was determined by MTT colorimetric analysis, lP3 content was determined by radioimmunoassay, Ca2+ concentration in cell by fluorometry and PKC activity by isotopic enzymolysis. The expression of gastrin, c-myc,C-fos and rasP21 in 48 cases of colorectal carcinoma tissue was detected by the immunocytochemistry SP method. Argyrophilianucleolar organizer regions was determined withargyrophilia stain.RESULTS The volume, weight, cAMP, DNAand protein content in carcinoma cell, cellamount and proliferation index of S and G2Mphase in PG group were all significantly higher than those of control group. When PG was at theconcentration of 25 mg/ L, the amount of viablecells, lP3 content and Ca2+ concentration in celland membrane PKC activity in PG group weresigniticantly higher than those in control group;when PGL was at a concentration ot 32 mg/L,they dropped to the lowest level in PG (25 mg/L)+ PGL group, but without significant differencefrom the control group. The positive expression rate of gastrin, c-myc, c-fos and rasp21 in carcinoma tissue was 39 .6%, 54 .2%, 47. 9% and54 .2% resPectively and significantly higher than that in mucosa 3 cm and 6 cm adjacent tocarcinoma tissue and normal colorectal mucosa.The positive expression rate of gastrin of highlydifferentiated adenocarcinoma group was significantly higher than that of poorly differentiated and mucinous adenocarcinoma groups. The AgNORs count of carcinoma tissue was significantly higher than that in mucosa 3 cm and 6 cm adjacent to carcinoma tissue and normal colorectal mucosa; and the positive expression of c-myc and c-fos and the AgNORs count in gastrin-positive group was significantly higher than those in gastrin-negative group.CONCLUSION Pentagastrin has a promoting effect on the growth of transplanted human colonic carcinoma from SW480 cell line. PGL hasno obvious effect on the growth of human colonic carcinoma SW480 cell line, but couldinhibit the growth-promoting effect of PG on transplanted carcinoma. Somatostatin can not only inhibit the growth of transplanted human colonic carcinoma from SW480 cell line directlybut also depress the growth-promoting effect ofgastrin on the transplanted carcinoma. Somecolorectal carcinoma cells can produce and secrete gastrin through autocrine, highly-differentiated adenocarcinoma express the highest level gastrin. Endogenous gastrin can stimulate the cell division and proliferation of carcinoma cell and promote the growth of colorectal carcinoma regulating the expression of oncogene omyc, c-fos. Our study has provided experimental basis for the adjuvant treatment using gastrin antagonist such as PGL,somatostatin of patients with colorectalcarcinoma. | He SW Shen KQ He YJ Xie B Zhao YM | 1999 | World Journal of Gastroenterology1999，5，5： | 20 |
| 2 | 心脏病及非心脏病患者降低舒张压的效果:收缩压干预试验结果分析显示文摘收缩压和舒张压低于何种阈值时心血管事件增多仍然不明确。应用收缩压干预试验(systolic blood pressure intervention trial,SPRINT)的数据,研究者评估了1519例心血管病患者和7574位非心血管病者的收缩压和舒张压与心血管事件之间的关系。 | 刘青 叶鹏 Khan NA Rabkin SW Zhao Y McAlister FA Park JE Guan M Chan S Humphries KH | 2018 | 中华高血压杂志2018，26，6： | 3 |
| 3 | ELECTROWINNING ZINC FROM CHLORIDE MELTS IN BIPOLAR CELL显示文摘ELECTROWINNINGZINCFROMCHLORIDEMELTSINBIPOLARCELLZhao;Guangwen(DepartmentofPhysicalChemistry,UniversityofScienceandTechnoloygB... | Zhao Guangwen(Department of Physical Chemistry ,University of Science and Technoloyg Beijing , Beijing 100083) Inman D(Imperial College, London SW 7 2BP) | 1995 | 中国有色金属学会会刊:英文版1995，5，1： | 1 |
| 4 | Hepatitis C virus F protein up-regulates c-myc and down-regulates p53 in human hepatoma hepG(2)cells显示文摘 | WU WB SHAO SW ZHAO LJ | 2007 | Intervirology2007，50，5： | 1 |
| 5 | Theaflavin derivatives in black tea and catechin derivatives in green tea inhibit HIV - 1 entry by targeting gp41显示文摘 | Liu SW Lu H Zhao Q | 2005 | Biochimica et Biophysica Acta2005，1723，13： | 1 |
| 6 | Preparation of protein conjugates via intermolecular hydrazone linkage显示文摘 | King TP Zhao SW Lam T | 1986 | Biochemistry1986，25，19： | 1 |
| 7 | EGFR mutations are associated with prognosis but not with the response to front-line chemothera- py in the Chinese patients with advanced non-small cell lung cancer 显示文摘 | Wu M Zhao J Song SW | 2010 | Lung Cancer2010，67，3： | 1 |
| 8 | EGFR mutations are associated with prognosis but not with the response to front-line chemotherapy in the Chinese patients with advanced non-small cell lung cancer显示文摘 | Wu M Zhao J Song SW | 2010 | Lung Cancer2010，67，3： | 1 |
| 9 | Effects of cyanobacteria bloom extract on some parameters of immune function in mice 显示文摘 | Shen PP Zhao SW Zheng WJ | 2003 | Toxicology Letters2003，143，： | 1 |
| 10 | BJU Int,Efficacy and safety of avanafil for treating erectile dysfunction:results of a multicentre,randomized,double-blind,placebo-controlled trial显示文摘 | Zhao C Kim SW Yang DY | 2012 | 110(11):1801-18062012，110，11： | 1 |
| 11 | Effects of cyanobacteria bloom extract on some parameters of immune function in mice 显示文摘 | Shen PP Zhao SW Zheng WJ | 2003 | Toxicol Lett2003，143，1： | 1 |
| 12 | PARP1 poly(ADP-ribosyl)ates Sox2 to control Sox2 protein levels and FGF4 expression during embryonic stem cell differentiation显示文摘 | Gao FR Kwon SW Zhao YM | | 0，，33： | 1 |
| 13 | Hepatitis C virus F protein up-regulates c-myc and down-regulates p53 in human hepatoma hepG2 cells 显示文摘 | Wu WB Shao SW Zhao LJ etal | 2007 | Intervirology2007，50，5： | 1 |
| 14 | Resonance Raman measurement of macular carotenoids in normal subjects and in age-related macular degeneration patients显示文摘 | Bernstein PS Zhao DY Wintch SW | 2002 | Ophthalmology2002，109，： | 1 |
| 15 | Theaflavin Derivatives in Black Tea and Catechin Derivatives in Green Tea Inhibit HIV-1 Entry by Targeting Gp41显示文摘 | Liu SW Lu H Zhao Q | 2005 | BBA2005，1723，13： | 1 |
| 16 | Hepatitis C virus F protein up- regulates e-myc and down-regulates p53 in human hepatoma HepG2 ceils 显示文摘 | Wu WB Shao SW Zhao LJ | 2007 | Intervirology2007，50，5： | 1 |
| 17 | Research on the Anti--aging Effects of Wild Purslane on Rabbits显示文摘 | He SW Zhao RH He SP | 1997 | Chinese Journal of Integrated Tradi- tional and Western medicine1997，17，7： | 1 |
| 18 | Vasculogenic mimicry is associated with shorter survival in hepatocellular carcinomas显示文摘 | Sun BC Zhang SW Zhao XL | | Laboratory Investigation0，，： | 1 |
| 19 | Lysine-5 acetylation negatively regulates lactate dehydrogenase A and is decreased in pancreatic cancer显示文摘 | Zhao D Zou SW Liu Y | 2013 | Cancer Cell2013，23，4： | 1 |
| 20 | Pax6 regulates proliferation and apoptosis of human retinoblastoma cells 显示文摘 | Li B Bai SW Zhang H Jonas JB Zhao BW Shen L | 2011 | Invest Ophthalmol Vis Sci2011，52，7： | 1 |
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