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| 1 | Spatial-resolved metabolomics reveals tissue-specific metabolic reprogramming in diabetic nephropathy by using mass spectrometry imaging显示文摘Detailed knowledge on tissue-specific metabolic reprogramming in diabetic nephropathy(DN)is vital for more accurate understanding the molecular pathological signature and developing novel therapeutic strategies.In the present study,a spatial-resolved metabolomics approach based on air flowassisted desorption electrospray ionization(AFADESI)and matrix-assisted laser desorption ionization(MALDI)integrated mass spectrometry imaging(MSI)was proposed to investigate tissue-specific metabolic alterations in the kidneys of high-fat diet-fed and streptozotocin(STZ)-treated DN rats and the therapeutic effect of astragalosideⅣ,a potential anti-diabetic drug,against DN.As a result,a wide range of functional metabolites including sugars,amino acids,nucleotides and their derivatives,fatty acids,phospholipids,sphingolipids,glycerides,carnitine and its derivatives,vitamins,peptides,and metal ions associated with DN were identified and their unique distribution patterns in the rat kidney were visualized with high chemical specificity and high spatial resolution.These region-specific metabolic disturbances were ameliorated by repeated oral administration of astragaloside Ⅳ(100 mg/kg)for 12 weeks.This study provided more comprehensive and detailed information about the tissue-specific metabolic reprogramming and molecular pathological signature in the kidney of diabetic rats.These findings highlighted the promising potential of AFADESI and MALDI integrated MSI based metabolomics approach for application in metabolic kidney diseases. | Zhonghua Wang Wenqing Fu Meiling Huo Bingshu He Yaqi Liu Lu Tian Wanfang Li Zhi Zhou Baili Wang Jianzhen Xia Yanhua Chen Jinfeng Wei Zeper Abliz | 2021 | Acta Pharmaceutica Sinica B2021，11，11： | 7 |
| 2 | Rapid and sensitive liquid chromatography–tandem mass spectrometric method for the quantitative determination of potentially harmful substance 5,5′-oxydimethylenebis (2-furfural) in traditional Chinese medicine injections显示文摘With the rapid development and wide application of traditional Chinese medicine injection(TCMI), a number of adverse events of some TCMIs have incessantly been reported and have drawn broad attention in recent years. Establishing effective and practical analytical methods for safety evaluation and quality control of TCMI can help to improve the safety of TCMIs in clinical applications. In this study, a sensitive and rapid high-performance liquid chromatography–tandem mass spectrometry(HPLC–MS/MS)method has been developed and validated for the quantitative determination of potentially harmful substance5,5′-oxydimethylenebis(2-furfural, OMBF) in TCMI samples. Chromatographic separation was performed on a C18 reversed-phase column(150 mm × 2.1 mm, 5 μm) by gradient elution, using methanol–water containing 0.1% formic acid as mobile phase at the flow rate of 0.3 m L/min. MS/MS detection was performed on a triple quadrupole mass spectrometer with positive electrospray ionization in the multiple reaction-monitoring mode. The method was sensitive with a limit of quantification of 0.3 ng/m L and linear over the range of 0.3–30 ng/m L(r = 0.9998). Intra-and inter-day precision for analyte was o9.52% RSD withrecoveries in the range 88.0–109.67% at three concentration levels. The validated method was successfully applied to quantitatively determine the compound OMBF in TCMIs and glucose injections. Our study indicates that this method is simple, sensitive, practicable and reliable, and could be applied for safety evaluation and quality control of TCMIs and glucose injections. | Qingce Zang Yang Gao Luojiao Huang Jiuming He Sheng Lin Hongtao Jin Ruiping Zhang Zeper Abliz | 2018 | Acta Pharmaceutica Sinica B2018，8，2： | 4 |
| 3 | A high-performance bio-tissue imaging method using air flow-assisted desorption electrospray ionization coupled with a high-resolution mass spectrometer显示文摘Mass spectrometry imaging (MSI) technology can simultaneously obtain the spatial distribution of thousands of chemical compounds and has unique advantages compared to other techniques that allow mapping the surface of bio-tissue. Here, we combined an air flow-assisted desorption electrospray ionization (AFADESI) MSI device with a high-resolution mass spectrometer to optimize the system parameters and achieve more accurate spatial distribution characteristics for compounds of interest while investigating bio-tissue sections. The platform set-up, required instrumentation, sample pretreatment, parameter optimization and bio-tissue characterization are described and discussed.Finally, the parameter conditions that can provide optimal ionic intensity and enhanced resolution were confirmed. The reasonable resolution and sensitivity improvements of AFADESI-MSI have been achieved through tandem a high-resolution mass spectrometer system, therefore, it would be a promising technique for the bio-tissue imaging analysis. | Yiwei Lv Tiegang Li Chengan Guo Chenglong Sun Fei Tang Luojiao Huang Zhigang Luo Xin Li Ruiping Zhang Qingce Zang Jiuming He Zeper AbliZ | 2019 | Chinese Chemical Letters2019，30，2： | 2 |
| 4 | Spatiotemporal pharmacometabolomics based on ambient mass spectrometry imaging to evaluate the metabolism and hepatotoxicity of amiodarone in HepG2 spheroids显示文摘Three-dimensional(3D)cell spheroid models combined with mass spectrometry imaging(MSI)enables innovative investigation of in vivo-like biological processes under different physiological and pathological conditions.Herein,airflow-assisted desorption electrospray ionization-MSI(AFADESI-MSI)was coupled with 3D HepG2 spheroids to assess the metabolism and hepatotoxicity of amiodarone(AMI).High-coverage imaging of>1100 endogenous metabolites in hepatocyte spheroids was achieved using AFADESI-MSI.Following AMI treatment at different times,15 metabolites of AMI involved in Ndesethylation,hydroxylation,deiodination,and desaturation metabolic reactions were identified,and according to their spatiotemporal dynamics features,the metabolic pathways of AMI were proposed.Subsequently,the temporal and spatial changes in metabolic disturbance within spheroids caused by drug exposure were obtained via metabolomic analysis.The main dysregulated metabolic pathways included arachidonic acid and glycerophospholipid metabolism,providing considerable evidence for the mechanism of AMI hepatotoxicity.In addition,a biomarker group of eight fatty acids was selected that provided improved indication of cell viability and could characterize the hepatotoxicity of AMI.The combination of AFADESI-MSI and HepG2 spheroids can simultaneously obtain spatiotemporal information for drugs,drug metabolites,and endogenous metabolites after AMI treatment,providing an effective tool for in vitro drug hepatotoxicity evaluation. | Limei Li Qingce Zang Xinzhu Li Ying Zhu Shanjing Wen Jiuming He Ruiping Zhang Zeper Abliz | 2023 | Journal of Pharmaceutical Analysis2023，13，5： | 2 |
| 5 | A rapid and sensitive UPLC–MS/MS method for quantitative determination of arformoterol in rat plasma, lung and trachea tissues显示文摘A rapid and sensitive ultra-performance liquid chromatography-tandem mass spectrometry(UPLC–MS/MS) method was developed and fully validated for determination of arformoterol in rat plasma, lung and trachea tissues. The chromatographic separation was performed on an Agilent XDB C8 column with gradient elution by using acetonitrile and 0.1% formic acid water. The method presented high sensitivity(LLOQ of 1.83 pg/mL for plasma and 3.90 pg/mL for lung and trachea tissue homogenates) and great linearity over the range of 1.83–458 pg/mL for plasma, 13.9–1560 pg/mL for lung and trachea tissue homogenates. No carry over effect and matrix effect were observed. The intra-and inter-day precision/accuracy were within ±15% at three quality control concentration levels. This robust method was successfully applied to the pharmacokinetic, lung and trachea tissue distribution study after inhalation administration of arformoterol tartrate inhalation solution(50 m g/kg). The in vivo information indicate that arformoterol can be rapidly absorbed into blood through respiratory systems, lung and trachea tissue maintain a certain amount of arformoterol in 1 h after dosing. | Chenglong Sun Jiuming He Hongbo Wu Xiaowei Song Jiangshuo Li Luojiao Huang Xiaoyu Xu Manjiangcuo Wang Ruiping Zhang Zeper Abliz | 2018 | Chinese Chemical Letters2018，29，8： | 2 |
| 6 | Design and performance of air flow-assisted ionization imaging mass spectrometry system显示文摘The imaging mass spectrometry(IMS) technology has experienced a rapid development in recent years.A new IMS technology which is based on air flow assisted ionization(AFAI) was reported.It allows for the convenient pretreatment of the samples and can image a large area of sample in a single measurement with high sensitivity.The AFAI in DESI mode was used as the ion source in this paper.The new IMS method is named AFADESI-IMS.The adoption of assisted air flow makes the sample pretreatment easy and convenient.An optimization of the distance between the ion transport tube and MS orifice increases the sensitivity of the system.For data processing,a program based on MATLAB with the function of numerical analysis was developed.A theoretical imaging resolution of a few hundred microns can be achieved.The composite AFAI-IMS images of different target analytes were imaged with high sensitivity.A typical AFAI-IMS image of the whole-body section of a rat was obtained in a single analytical measurement.The ability to image a large area for relevant samples in a single measurement with high sensitivity and repeatability is a significant advantage.The method has enormous potentials in the MS imaging of large and complicated samples. | Fei Tang Yi Chen Jiu-Ming He Zhi-Gang Luo Zeper Abliz Xiao-Hao Wang | 2014 | Chinese Chemical Letters2014，25，5： | 2 |
| 7 | A quick and effective multivariate statistical strategy for imaging mass spectrometry显示文摘A new multivariate statistical strategy for analyzing large datasets that are produced by imaging mass spectrometry(IMS) techniques is reported.The strategy divides the whole datacube of the sample into several subsets and analyses them one by one to obtain the results.Instead of analyzing the whole datacube at one time,the strategy makes the analysis easier and decreases the computation time greatly.In this report,the IMS data are produced by the air flow-assisted ionization IMS(AFAI-IMS).The strategy can be used in combination with most multivariate statistical analysis methods.In this paper,the strategy was combined with the principal component analysis(PCA) and partial least square analysis(PLS).It was proven to be effective by analyzing the handwriting sample.By using the strategy,the m/z corresponding to the specific lipids in rat brain tissue were distinguished successfully.Moreover the analysis time grew linearly instead of exponentially as the size of sample increased.The strategy developed in this study has enormous potential for searching for the mjz of potential biomarkers quickly and effectively. | Fei Tang Yi Chen Tie-Gang Li Jiu-Ming He Zeper Abliz Gang Huang Xiao-Hao Wang | 2014 | Chinese Chemical Letters2014，25，10： | 1 |
| 8 | Structural characterization of flavonol 3,7-di-O-glycosides and determination of the glycosylation position by using negative ion electrospray i- onization tandem mass spectrometry 显示文摘 | Keyume Ablajan Zeper Abliz Xiao-Ya Shang | 2006 | J Mass Speetrom2006，41，： | 1 |
| 9 | Using Nuclear Magnetic Resonance and MS/MS Spectroscopy for the Identification of Brodimoprim Metabolites in Rat Urine显示文摘Eight metabolites of brodimoprim (BDP) in rat urine were detected by NMR and ESI-MS/MS. They were demethyl-BDP glucuronide, demethyl-BDP sulfurate, demethyl-BDP glucuronide sulfurate, (-hydroxyl-BDP, (-hydroxyl-BDP glucuronide, BDP sulfurate, N-oxide-BDP sulfurate, and (-hydroxyl-N-oxide-BDP sulfurate. All the sulfurates are reported for the first time. | Chun YANG, Wen Yi HE , Li Jun LI, Rui Ming XU, Shou Ren ZHANG, Zeper ABLIZ, Yi Kang SI* Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050 | 2001 | Chinese Chemical Letters2001，12，6： | 1 |
| 10 | Structural characteriza-tion of steriodal saponins by electrospray ionization and fast - atombombardment tandem mass spectrometry 显示文摘 | Feng Liang Li - jun Li Zeper Abliz | 2002 | Rapid communicationsin mass spectrometry2002，16，： | 1 |
| 11 | Acta Pharmaceutica Sinica显示文摘 | Chang Yan Abliz Zeper Wang Muzou | 2000 | 35 (1) : 732000，35，1： | 1 |
| 12 | Study on fragmentation behavior of taxoids by tandem mass spectrometry显示文摘We have studied the fragmentation behavior of positive and negative ions of taxoi and 6/8/6 type taxoids, the influence of different substituents on fragmentation, and the correlativity between fragmentation patterns and structure by MS/MS technique with different ionization methods such as FAB-MS, ESI-MS, ete. We have also investigated in detail the fragmentation of various molecular-related ions, such as [M+H]+, [M+Na]+ and [M-H] ions, and the formation pathways of characteristic fragment ions. It has been found that there exists some competing reaction between the loss of C-13 side chain and decomposition by loss of acetic acid. In addition, by comparing CID spectra obtained with low- and high-energy collision, it is seen that CID-MS/MS with low-energy collision is more suitable for the study of the structural analysis of small molecules and drug metabolites. The experimental results demonstrate that MS/MS spectra can reflect more effectively the slight difference of structure between the related | Zeper Abliz Mitsuo Takayama | 2000 | Chinese Science Bulletin2000，45，8： | 1 |
| 13 | Self-assembly of Pyrene-modified Rhomboidal Metalloden- drimers via Directional Metal-ligand Bonding Approach显示文摘设计并且经由方向性的 metal-ligand 的修改分核的长斜方形的 metallodendrimers R1R6 自己组装结合的途径被描述。由采用包含分核 120 敺搠獩牴扩瑵潩 ? 景 ? 偄华 ? 敷敲搠瑥牥業敮 ? 祢搠湹浡捩氠杩? | Menglan He Qing Han Jiuming He Quanjie Li Zeper Abliz Hongwei Tan Lin Xu Haibo Yang | 2013 | Chinese Journal of Chemistry2013，31，5： | 1 |
| 14 | On-tissue chemical derivatization enables spatiotemporal heterogeneity visualization of oxylipins in esophageal cancer xenograft via ambient mass spectrometry imaging显示文摘On-tissue chemical derivatization(OTCD)effectively enhances ionization efficiency of low abundant and poorly ionized functional molecules to improve detection sensitivity and coverage of mass spectrometry imaging(MSI).Combination OTCD and MSI provides a novel strategy for visualizing previously undisclosed metabolic heterogeneity in tumor.Herein,we present a method to visualize heterogeneous metabolism of oxylipins within tumor by coupling OTCD with airflow-assisted desorption electrospray ionization(AFADESI)-MSI.Taking Girard’s P as a derivatization reagent,easily ionized hydrazide and quaternary amine groups were introduced into the structure of carbonyl metabolites via condensation reaction.Oxylipins,including 127 fatty aldehydes(FALs)and 71 oxo fatty acids(FAs),were detected and imaged in esophageal cancer xenograft with AFADESI-MSI after OTCD.Then t-distributed stochastic neighbor embedding and random forest were exploited to precisely locate the distribution of oxylipins in heterogeneous tumor tissue.With this method,we surprisingly found almost all FALs and oxo FAs significantly accumulated in the core region of tumor,and exhibited a gradual increase trend in tumor over time.These results reveal spatiotemporal heterogeneity of oxylipins in tumor progression,highlighting the value of OTCD combined with MSI to gain deeper insights into understanding tumor metabolism. | Jialin Liu Qingce Zang Xinzhu Li Xinyi Tu Ying Zhu Lingzhi Wang Zitong Zhao Yongmei Song Ruiping Zhang Zeper Abliz | 2023 | Chinese Chemical Letters2023，34，11： | 0 |
| 15 | Direct determination of trace amounts of acetic acid using a novel ambient glow discharge ion source显示文摘A novel ambient glow discharge ion source with improved line-cylinder electrodes is put forward and designed in this paper.The diameters of inner and outer electrodes are 0.16 and 4 mm respectively.With a special assembly method,a perfect coaxiality of the two electrodes is obtained.From the gas discharge experiment,it can be seen that the discharge can stably work in normal glow discharge mode.The operating currents of the ion source are in an order of milliamperes and can generate a much larger number and wider variety of reagent ions.The MS experiment shows that the ion source has higher detection sensitivity. | Xiao Hao Wang Kun Liu Fei Tang Jiu Ming He Xue Ye Wei Zeper Abliz | 2010 | Chinese Chemical Letters2010，21，10： | 0 |
| 16 | EVIDENCE FOR AROMATIC sp^2 C-H…O=C INTRAMOLECULAR INTERACTION IN PYRIDINO AND BENZOBENZANTHRONE SKELETONS显示文摘EVIDENCEFORAROMATICsp~2C-H…O=CINTRAMOLECULARINTERACTIONINPYRIDINOANDBENZOBENZANTHRONESKELETONS￥ZeperABLIZ(DepartmentofChemist?.. | Zeper ABLIZ (Department of Chemistry, Xin Jiang University, Urumchi 830046) Hiroshi MORIYAMA and Junji AOKI (Department of Chemistry, Toho University, Funabashi, Chiba 274, Japan) Toyotoshi UEDA (Department of Chemistry, Meisei University, Hino, Tokyo 19 | 1995 | Chinese Chemical Letters1995，6，5： | 0 |
| 17 | Integrated mass spectrometry imaging reveals spatial-metabolic alteration in diabetic cardiomyopathy and the intervention effects of ferulic acid显示文摘Diabetic cardiomyopathy(DCM)is a metabolic disease and a leading cause of heart failure among people with diabetes.Mass spectrometry imaging(MSI)is a versatile technique capable of combining the molecular specificity of mass spectrometry(MS)with the spatial information of imaging.In this study,we used MSI to visualize metabolites in the rat heart with high spatial resolution and sensitivity.We optimized the air flow-assisted desorption electrospray ionization(AFADESI)-MSI platform to detect a wide range of metabolites,and then used matrix-assisted laser desorption ionization(MALDI)-MSI for increasing metabolic coverage and improving localization resolution.AFADESI-MSI detected 214 and 149 metabolites in positive and negative analyses of rat heart sections,respectively,while MALDI-MSI detected 61 metabolites in negative analysis.Our study revealed the heterogenous metabolic profile of the heart in a DCM model,with over 105 region-specific changes in the levels of a wide range of metabolite classes,including carbohydrates,amino acids,nucleotides,and their derivatives,fatty acids,glycerol phospholipids,carnitines,and metal ions.The repeated oral administration of ferulic acid during 20 weeks significantly improved most of the metabolic disorders in the DCM model.Our findings provide novel insights into the molecular mechanisms underlying DCM and the potential of ferulic acid as a therapeutic agent for treating this condition. | Yanhua Liu Xin Zhang Shu Yang Zhi Zhou Lu Tian Wanfang Li Jinfeng Wei Zeper Abliz Zhonghua Wang | 2023 | Journal of Pharmaceutical Analysis2023，13，12： | 0 |
| 18 | Existence of aromatic sp^2 C-H…O = C intramolecular interaction similar to hydrogen bond显示文摘A ’H NMR signal shifted drastically to down field (δ - 10.0) at the bay area and in dose proximity to C = O group for H-1 was observed through complete assignments of 1H NMR spectra for pyridino- and benzobenzanthrones. It is concluded that this phenomenon is due not only to the anisotropy effect of C=O plus aromatic ring current effect, but also to the electrostatic attraction of C-H (δ+)…O(δ-)=C interaction. The evidence for the sp2C-H…O = C intramolecular interaction similar to hydrogen bond has also been given by EI-MS and MS/MS (CID) spectroscopy and IR spectroscopy, as well as MNDO-PM3 calculations. This new kind of interaction might be called ’quasi-hydrogen bond’. | Zeper Abliz Hiroshi Moriyama Junji Aoki Toyotoshi Ueda | 1996 | Science China Chemistry1996，39，3： | 0 |
| 19 | Discrimination of Structural Isomers of Aromatic Compounds with EI Mass Spectra and Their Ionization Efficiency Curves of Dehydrogenated Fragment Ions显示文摘The mechanism of ionization and fragmentation for terphenyl (diphenylbenzene) withthree structural ring isomers (ortho-, meta- and para-), and stilbene (1,2-diphenylethylene) with two geometrical isomers (trans- and ets-) by EI mass spectrometry and ionizstion efficiency curves are investigation. | Zeper ABLIZ Li Jun LI Teruo KAN and Toyotoshi UEDA(Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050Tokyo Metropolitan Research Laboratory of Public Health, Shinjuku-ku, Tokyo 160, JapanDepa | 1998 | Chinese Chemical Letters1998，9，10： | 0 |
| 20 | Spatiotemporally resolved metabolomics and isotope tracing reveal CNS drug targets显示文摘Deconvolution of potential drug targets of the central nervous system(CNS)is particularly challenging because of the complicated structure and function of the brain.Here,a spatiotemporally resolved metabolomics and isotope tracing strategy was proposed and demonstrated to be powerful for deconvoluting and localizing potential targets of CNS drugs by using ambient mass spectrometry imaging.This strategy can map various substances including exogenous drugs,isotopically labeled metabolites,and various types of endogenous metabolites in the brain tissue sections to illustrate their microregional distribution pattern in the brain and locate drug action-related metabolic nodes and pathways.The strategy revealed that the sedative-hypnotic drug candidate YZG-331 was prominently distributed in the pineal gland and entered the thalamus and hypothalamus in relatively small amounts,and can increase glutamate decarboxylase activity to elevateγ-aminobutyric acid(GABA)levels in the hypothalamus,agonize organic cation transporter 3 to release extracellular histamine into peripheral circulation.These findings emphasize the promising capability of spatiotemporally resolved metabolomics and isotope tracing to help elucidate the multiple targets and the mechanisms of action of CNS drugs. | Bo Jin Xuechao Pang Qingce Zang Man Ga Jing Xu Zhigang Luo Ruiping Zhang Jiangong Shi Jiuming He Zeper Abliz | 2023 | Acta Pharmaceutica Sinica B2023，13，4： | 0 |
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