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| 1 | Identification of colorectal cancer metastasis markers by an angiogenesis-related cytokine-antibody array显示文摘AIM:To investigate the angiogenesis-related protein expression profile characterizing metastatic colorectal cancer (mCRC) with the aim of identifying prognostic markers.METHODS:The expression of 44 angiogenesissecreted factors was measured by a novel cytokine antibody array methodology.The study evaluated vascular endothelial growth factor (VEGF) and its soluble vascular endothelial growth factor receptor (sVEGFR)-1 protein levels by enzyme immunoassay (EIA) in a panel of 16 CRC cell lines.mRNA VEGF and VEGF-A isoforms were quantified by quantitative reverse-transcription polymerase chain reaction (Q-RT-PCR) and vascular endothelial growth factor receptor (VEGFR)-2 expression was analyzed by flow cytometry.RESULTS:Metastasis-derived CRC cell lines expressed a distinctive molecular profile as compared with those isolated from a primary tumor site.Metastatic CRC cell lines were characterized by higher expression of angiogenin-2 (Ang-2),macrophage chemoattractant proteins-3/4 (MCP-3/4),matrix metalloproteinase-1 (MMP-1),and the chemokines interferon γ inducible T cell α chemoattractant protein (I-TAC),monocyte chemoattractant protein I-309,and interleukins interleukin (IL)-2 and IL-1α,as compared to primary tumor cell lines.In contrast,primary CRC cell lines expressed higher levels of interferon γ (IFN-γ),insulin-like growth factor-1 (IGF-1),IL-6,leptin,epidermal growth factor (EGF),placental growth factor (PlGF),thrombopoietin,transforming growth factor β1 (TGF-β1) and VEGF-D,as compared with the metastatic cell lines.VEGF expression does not significantly differ according to the CRC cellular origin in normoxia.Severe hypoxia induced VEGF expression up-regulation but contrary to expectations,metastatic CRC cell lines did not respond as much as primary cell lines to the hypoxic stimulus.In CRC primary-derived cell lines,we observed a twofold increase in VEGF expression between normoxia and hypoxia as compared to metastatic cell lines.CRC cell lines express a similar pattern of VEGF isoforms (VEGF 121,VEGF 165 and VEGF 189) despite variability in VEGF expression,where the major transcript was VEGF 121.No relevant expression of VEGFR-2 was found in CRC cell lines,as compared to that of human umbilical vein endothelial cells and sVEGFR-1 expression did not depend on the CRC cellular origin.CONCLUSION:A distinct angiogenesis-related expression pattern characterizes metastatic CRC cell lines.Factors other than VEGF appear as prognostic markers and intervention targets in the metastatic CRC setting. | Ana Abajo Nerea Bitarte Ruth Zarate Valentina Boni Ines Lopez Marisol Gonzalez-Huarriz Javier Rodriguez Eva Bandres Jesus Garcia-Foncillas | 2012 | World Journal of Gastroenterology2012，18，7： | 8 |
| 2 | Physiology, Injury, and Recovery of Interstitial Cells of Cajal: Basic and Clinical Science显示文摘 | Jan D. Huizinga Natalia Zarate Gianrico Farrugia | 2009 | Gastroenterology2009，，5： | 2 |
| 3 | Effects of feeding frequency and rate of stomach evacuation on utilization of dietary free and protein-bound lysine for growth by channel catfish Ictalurus punctatus显示文摘 | ZARATE D D LOVELL R T PAYNE M | 1999 | Aquaculture Nutrition1999，5，： | 1 |
| 4 | A randomized trial of an N-methyl-D-aspartate antagonist in treatment-resistant major depression 显示文摘 | Zarate C A Singh J B Carlson P J | 2006 | Arch Gen Psychiatry2006，63，： | 1 |
| 5 | Antimicrobial activity of lactic acid bacteria isolated from Tenerife cheese:initial characterization of plantaricin TF711,a bacteriocin-like substance produced by Lactobacillus plantarum TF711显示文摘 | HEMANDEZ D CARDLE E E ZARATE V | 2005 | J Appl Microbiol2005，99，1： | 1 |
| 6 | Terpenoid indole alkaloid profile changes in Catharanthus pusillus during development显示文摘 | Zarate R Dirks C van der Heijden R Verpoorte R | 2001 | Plant Sci2001，160，5： | 1 |
| 7 | Structural analysis of nanocrystalline BaTiO3显示文摘 | CHA VEZ E FUENTES S ZARATE R A | 2010 | Journal of Molecular Structure2010，984，123： | 1 |
| 8 | Silverleaf whitefly in- duces salicylic acid defenses and suppresses effectual jasmonic acid defenses 显示文摘 | Zarate S I Kempema L A Walling L L | 2007 | Plant Physiology2007，143，： | 1 |
| 9 | Cellular mechanisms underlying the antidepressant effects of ketamine;role of alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptors显示文摘 | Maeng S Zarate CA Jr Du J | | 0，，04： | 1 |
| 10 | Neutron and gamma dose equivalent rates in the vicinity of a self-shielded PET cyclotron after an upgrade with increased beam current显示文摘 | AVILARODRIGUEZ M AVENDANESTRADA A ZARAT EMORALES A | 2011 | J Nucl Med Meeting Abstracts2011，52，1： | 1 |
| 11 | Relationship of ghrelin,acid uric and proinflammatory adipocytokines in different degrees of obesity or diabetes显示文摘 | Cruz-Domínguez MP Cortés DH Zarate A | 2014 | Int J Clin Exp Med2014，7，5： | 1 |
| 12 | Recent advances in social skills training for schizophrenia显示文摘 | Kopelowicz A Liberman RP Zarate R | 2006 | Schizophr Bull2006，32，1： | 1 |
| 13 | Growth and electron field-emission of single-crystal line ZnO nanowires显示文摘 | MOSQUERA E BERNAL J ZARATE R A | 2013 | Mater Lett2013，93，： | 1 |
| 14 | Targeting the glutamatergic system to treat major depressive disorder: rationale and progress to date显示文摘 | Mathews DC Henter ID Zarate CA | 2012 | Drugs2012，72，10： | 1 |
| 15 | Defining anxious depression: a review of the literature显示文摘 | Dawn F. Ionescu Mark J. Niciu Ioline D. Henter Carlos A. Zarate | 2013 | CNS Spectrums2013，，5： | 1 |
| 16 | Reproducible measurement of single-molecule conductivity显示文摘 | Cui X D Primak A Zarate X | 2001 | Science2001，294，： | 1 |
| 17 | Oxaliplatin, irinotecan and capecitabine as first-line therapy in metastatic colorectal cancer (mCRC): a dose-finding study and pharmacogenomic analysis显示文摘 | Zarate R Rodriguez J Bandres E | 2010 | Br J Cancer2010，102，6： | 1 |
| 18 | Novel insights into lithium's mechanism of action: neurotrophic and neuroprotective effects 显示文摘 | Quiroz JA Machado-Vieira R Zarate Jr CA | 2010 | Neuropsvehobiolog32010，62，1： | 1 |
| 19 | Recent advances in socialskills training for schizophrenia显示文摘 | Kopelowicz A Liberman RP Zarate R | 2006 | Schizophr Bull2006，32，： | 1 |
| 20 | A randomized trial of an N-methyl-D-Aspartate antagonist in treatmentresistant major depression显示文摘 | Zarate CA Jr Singh JB Carlson PJ | 2006 | Arch Gen Psychiatry2006，63，8： | 1 |
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