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2339篇 您的检索式:作者名="ZOU C"
    题名 作者 年代 出处 被引量
1虹鳟肿瘤坏死因子(TNFα)基因体外表达与纯化的研究显示文摘将虹鳟两种肿瘤坏死因子TNFα1和TNFα2基因的成熟肽编码区域 ,用带有BamHI和HindIII酶切位点的基因特异性引物进行PCR扩增。扩增片段用限制性内切酶消化并连接到pQE30表达载体上 ,连接产物转化到大肠杆菌JM10 9感受态细菌中。转化子经PCR筛选 ,质粒测序 ,完成了虹鳟两种TNFα基因重组子的构建。重组子经体外培养和诱导后 ,获得了高效表达的TNFα重组蛋白。高效表达的重组TNFα不受诱导剂IPTG的影响 ,并且由于重组子高效表达而形成了包涵体 ;重组蛋白产量约占菌体蛋白总量的 2 5 %— 30 %。应用Ni NTA和固定金属亲和层析 (IM PC)技术 ,在变性条件下获得了高度纯化的重组蛋白 ,纯化重组蛋白的产量约为 0 5— 1mg L。蔡中华 宋林生 ZOU Jun C J SECOMBES 董双林 2003水生生物学报2003,27,6:7
2A naturally occurring CD8^+CD122^+ T-cell subset as a memory-like Treg family显示文摘尽管有 CD4 + CD25 + 规章的 T 房间(Tregs ) 在过去的十年期间,在他们的临床的翻译以后的进步仍然保持停滞。增长证据建议那自然地发生的 CD8 + CD122 + T 房间也是有能力的 Tregs 禁止 T 房间回答并且象 alloimmunity 一样压制 autoimmunity。事实上,他们是像记忆的 Tregs 类似于一个中央记忆 T 房间(T 厘米 ) 显型。位于他们的抑制下面的机制仍然不好被理解,尽管他们可以包括 IL-10 生产。我们最近证明了那个规划 death-1 (PD-1 ) 表达式区分在之间规章并且存储器 CD8 + CD122 + T 房间和那 CD8 + CD122 + Tregs 经历更快的 homeostatic 增长并且比常规 CD4 + CD25 + Tregs。这些调查结果可以为在诊所加速有效 Treg 治疗打开调查的一根新线。在这评论,我们在 CD8 + CD122 + Treg 在诊所研究并且讨论他们的显型,在 autoimmunity 和 alloimmunity 的镇压角色,功能的要求,行动的机制和潜在的应用。Shanshan Li Qingfeng Xie Yuqun Zeng Chuan Zou Xusheng Liu Shouhai Wu Haixia Deng Yang Xu Xian C Li Zhenhua Dai 2014Cellular & Molecular Immunology2014,11,4:6
3A NEW CYCLOPEPTIDE FROM VACCARIA SEGETALIS显示文摘ANEWCYCLOPEPTIDEFROMVACCARIASEGETALIS¥RongPingZHANG;ChengZOU;YuKunCHAI;JunZHOU(LaboratoryofPhytochemistry,KunmingInstituteoFb...Zhang, RP Zou, C Chai, YK Zhou, J 1995Chinese Chemical Letters1995,6,8:6
4High-fat emulsion-induced rat model of nonalcoholic steatohepatitis显示文摘Zou Y Li J Lu C 2006Life Sci2006,79,:1
5High-pressure high-temperature X-ray diffraction of β-boron to 30GPa显示文摘Ma Y Z Prewitt C T Zou G T 2003Phys Rev B2003,67,17:1
6Abeta(25-35)-induced memory impairment,axonal atrophy,and synaptic loss are ameliorated by M1,A metabolite of protopanaxadiol-type saponins显示文摘Tohda C Matsumoto N Zou K 2004Neuropsychopharmacology2004,29,:1
7显示文摘Zou X C Fu X K Li Y D 2010Adv Synth Catal2010,352,:1
8Bacterial roles in the forma- tion of high molecular weight dissolved organic matter in estuarine and coastal waters: Evidence from lipids and the compound-specificisotopic ratios 显示文摘Zou L Wang X C Callahan J 2004Limnology and Oceanography2004,49,1:1
9Optimization of die profile for improving die life in the hot extrusion process显示文摘ZOU L XIA J C WANG X Y 2003Journal of Materials Processing Technology2003,142,:1
10Metabolites of Colletotrichum gloeosporioides,an eddophytic fungus in Artemisia mongolica显示文摘Zou W X Meng J C Lu H 2000J Nat Prod2000,63,:1
11Mechanical properties of single crystal tungsten microwhiskers characterized by nanoindentation 显示文摘HUANG H WU Y Q WANG S L HE Y H ZOU J HUANG B Y LIU C T 2009Materials Science and Engeering A2009,53,12:1
12Effect of irradiation time on riboflavin-ultraviolet-A collagen crosslinking in rabbit sclera显示文摘Zhang Y Zou C Liu L 2013J Cataract Refract Surg2013,39,:1
13Detection of Fabric Defects with Fuzzy Label Co-occurrence Matrix Set显示文摘Zou C Wang B W Sun Z G 2009Journal of Donghua University(English Version)2009,26,5:1
14A semi-fragile lossless digital watermarking scheme based on integer wavelet transform 显示文摘Zou D SHX Y Q NI Z C 2006IEEE Transactions on Circuits Systems for Video Technology2006,16,10:1
15Sensitive and rapid detection of the new Delhi metallo-beta- lactamase gene by loop-mediated isothermal amplification 显示文摘Liu W Zou D Li Y Wang X He X Wei X Shao C Li X Shang W Yu K Liu D Li Y Guo J Yin Z Yuan J 2012J Clin Microbiol2012,50,5:1
16Efficient inscription of Bragg gratings in As2S3 fibers using near bandgap light显示文摘ZOU L E KABAKOVA I V MAGI E C 2013Optics Letters2013,38,19:1
17Stochastic differential delay equations in population dynamics显示文摘Mao X R Yuan C Zou J 2005J Math Anal Appl2005,304,:1
18Synthesis of Bi6Mo2015sub-microwires via a molten salt method and enhancing thephotocatalytic reduction of CO2 into solar fuel through tuning the surface oxide vacancies by simple post-heating treatment显示文摘Li P Zhou Y Tu W Wang R Zhang C Liu Q Li H Li Z Dai H Wang J Yan S Zou Z 2013CrystEngComm2013,15,46:1
19Effects of a pain education program on nurses' pain knowledge, attitudes and pain as- sessment practices in China 显示文摘Zhang C Hsu L Zou B 2008J Pain Symptom Manage2008,36,6:1
20Study of a Three-stage reconnection electromagnetic launcher using triggered vacuum switches 显示文摘ZHAO C ZOU J Y LI X P 2007IEEE Transactions on Magnetics2007,43,1:1
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